KIAA0005 sequence was obtained by assembly of
sequences derived from two clones. ha00071 retains
sequence from nt. positions 298 to 2998 of KIAA0005.
The cDNA fragment was cloned by use of ZAP-cDNA
synthesis kit and inserted at the EcoRI-XhoI site of
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted
at the EcoRI site of the vector. The 3' end of the cDNA
was inserted at the XhoI site of the vector. The other
clone a263 harbors sequence from nt. positions 1 to 477
of KIAA0005 which was amplified by RACE. The RACE
product was cloned into the SmaI site of the M13mp18
vector (GenBank Accession No. X02513). Extra 15
residues were present between the SmaI site and
beginning of KIAA0005 sequence.
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