Vector Information

KIAA0005 sequence was obtained by assembly of 
sequences derived from two clones. ha00071 retains 
sequence from nt. positions 298 to 2998 of KIAA0005. 
The cDNA fragment was cloned by use of ZAP-cDNA 
synthesis kit and inserted at the EcoRI-XhoI site of 
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted 
at the EcoRI site of the vector. The 3' end of the cDNA 
was inserted at the XhoI site of the vector. The other 
clone a263 harbors sequence from nt. positions 1 to 477 
of KIAA0005 which was amplified by RACE. The RACE 
product was cloned into the SmaI site of the M13mp18 
vector (GenBank Accession No. X02513). Extra 15 
residues were present between the SmaI site and 
beginning of KIAA0005 sequence.
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