The cDNA fragment was inserted at the SalI-NotI site of
the pSPORT1 vector (GenBank Accession No. U12390, AmpR).
The 5' end of the cDNA was repaired and ligated with 
the SalI adapter (5'TCGACCCACGCGTCCG3') and 
inserted at the SalI site of the vector. The 3' end of the 
cDNA was inserted at the NotI site of the vector.