Restriction map for
pF1KF0017
(Linear) (Six-base) MAPSORT of: FLJ00017.seq Check: 9724 from: 1 to: 1182
FLJ00017.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 559 1182
Size: 559 623
AatII G_ACGT'C
Cuts at: 0 278 1182
Size: 278 904
Acc65I G'GTAC_C
Cuts at: 0 334 1182
Size: 334 848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 1182
Size: 127 1055
AfeI AGC'GCT
Cuts at: 0 634 1182
Size: 634 548
AflIII A'CryG_T
Cuts at: 0 557 1182
Size: 557 625
AlwNI CAG_nnn'CTG
Cuts at: 0 87 105 441 1182
Size: 87 18 336 741
Fragments arranged by size:
741 336 87 18
AvaI C'yCGr_G
Cuts at: 0 679 1182
Size: 679 503
BamHI G'GATC_C
Cuts at: 0 354 1182
Size: 354 828
BanI G'GyrC_C
Cuts at: 0 71 334 1182
Size: 71 263 848
Fragments arranged by size:
848 263 71
BanII G_rGCy'C
Cuts at: 0 23 971 1182
Size: 23 948 211
Fragments arranged by size:
948 211 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 525 826 1182
Size: 525 301 356
Fragments arranged by size:
525 356 301
BciVI GTATCCnnnnn_n'
Cuts at: 0 247 1182
Size: 247 935
BlpI GC'TnA_GC
Cuts at: 0 31 1182
Size: 31 1151
BmgBI CAC'GTC
Cuts at: 0 824 1182
Size: 824 358
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 744 939 1182
Size: 744 195 243
Fragments arranged by size:
744 243 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 361 985 1182
Size: 109 252 624 197
Fragments arranged by size:
624 252 197 109
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 934 1182
Size: 784 150 248
Fragments arranged by size:
784 248 150
BsaI GGTCTCn'nnnn_
Cuts at: 0 1152 1182
Size: 1152 30
BsaAI yAC'GTr
Cuts at: 0 558 1182
Size: 558 624
BsaHI Gr'CG_yC
Cuts at: 0 275 530 1182
Size: 275 255 652
Fragments arranged by size:
652 275 255
BsaWI w'CCGG_w
Cuts at: 0 482 1182
Size: 482 700
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 1182
Size: 175 1007
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1182
Size: 949 233
BsiHKAI G_wGCw'C
Cuts at: 0 257 971 1182
Size: 257 714 211
Fragments arranged by size:
714 257 211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 37 1182
Size: 37 1145
Bsp1286I G_dGCh'C
Cuts at: 0 23 257 971 1182
Size: 23 234 714 211
Fragments arranged by size:
714 234 211 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 360 374 559 683 1182
Size: 360 14 185 124 499
Fragments arranged by size:
499 360 185 124 14
BsrDI GCAATG_nn'
Cuts at: 0 284 756 1182
Size: 284 472 426
Fragments arranged by size:
472 426 284
BssSI C'ACGA_G
Cuts at: 0 397 1182
Size: 397 785
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 1182
Size: 261 921
BstYI r'GATC_y
Cuts at: 0 354 1182
Size: 354 828
Bsu36I CC'TnA_GG
Cuts at: 0 430 737 1182
Size: 430 307 445
Fragments arranged by size:
445 430 307
BtgI C'CryG_G
Cuts at: 0 23 651 895 1182
Size: 23 628 244 287
Fragments arranged by size:
628 287 244 23
EarI CTCTTCn'nnn_
Cuts at: 0 44 706 1182
Size: 44 662 476
Fragments arranged by size:
662 476 44
EcoICRI GAG'CTC
Cuts at: 0 969 1182
Size: 969 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 127 361 985 1182
Size: 109 18 234 624 197
Fragments arranged by size:
624 234 197 109 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 421 679 1182
Size: 421 258 503
Fragments arranged by size:
503 421 258
EcoO109I rG'GnC_Cy
Cuts at: 0 312 372 426 1182
Size: 312 60 54 756
Fragments arranged by size:
756 312 60 54
HaeII r_GCGC'y
Cuts at: 0 636 837 1182
Size: 636 201 345
Fragments arranged by size:
636 345 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 500 532 1103 1132 1135 1164 1182
Size: 500 32 571 29 3 29 18
Fragments arranged by size:
571 500 32 29 29 18 3
KpnI G_GTAC'C
Cuts at: 0 338 1182
Size: 338 844
MslI CAynn'nnrTG
Cuts at: 0 577 1182
Size: 577 605
MspA1I CmG'CkG
Cuts at: 0 25 38 653 690 1182
Size: 25 13 615 37 492
Fragments arranged by size:
615 492 37 25 13
PfoI T'CCnGG_A
Cuts at: 0 86 278 611 1182
Size: 86 192 333 571
Fragments arranged by size:
571 333 192 86
PpuMI rG'GwC_Cy
Cuts at: 0 372 1182
Size: 372 810
PstI C_TGCA'G
Cuts at: 0 371 1182
Size: 371 811
PvuII CAG'CTG
Cuts at: 0 690 1182
Size: 690 492
SacI G_AGCT'C
Cuts at: 0 971 1182
Size: 971 211
SacII CC_GC'GG
Cuts at: 0 26 654 1182
Size: 26 628 528
Fragments arranged by size:
628 528 26
SbfI CC_TGCA'GG
Cuts at: 0 371 1182
Size: 371 811
SexAI A'CCwGG_T
Cuts at: 0 1142 1182
Size: 1142 40
SfcI C'TryA_G
Cuts at: 0 367 1182
Size: 367 815
SmlI C'TyrA_G
Cuts at: 0 799 913 1182
Size: 799 114 269
Fragments arranged by size:
799 269 114
StuI AGG'CCT
Cuts at: 0 382 496 1006 1182
Size: 382 114 510 176
Fragments arranged by size:
510 382 176 114
StyI C'CwwG_G
Cuts at: 0 230 1182
Size: 230 952
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 29 865 1030 1182
Size: 29 836 165 152
Fragments arranged by size:
836 165 152 29
Tth111I GACn'n_nGTC
Cuts at: 0 1144 1182
Size: 1144 38
XmnI GAAnn'nnTTC
Cuts at: 0 700 955 1182
Size: 700 255 227
Fragments arranged by size:
700 255 227
ZraI GAC'GTC
Cuts at: 0 276 1182
Size: 276 906
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AlwNI AvaI
BamHI BanI BanII BbsI BciVI BlpI BmgBI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BssSI BstAPI BstYI
Bsu36I BtgI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KpnI MslI MspA1I PfoI PpuMI PstI PvuII
SacI SacII SbfI SexAI SfcI SmlI StuI StyI
TaqII Tth111I XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI AleI AlfI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BbvCI BcgI BclI BfrBI BglI BglII Bme1580I
BmrI BmtI Bpu10I BsaBI BsaXI BseYI BsiEI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SalI SanDI SapI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI TstI XbaI XcmI XhoI XmaI