Restriction map for
pF1KSDF0018
(Linear) (Six-base) MAPSORT of: FLJ00018.seq Check: 1377 from: 1 to: 4158
FLJ00018.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 420 1175 4158
Size: 420 755 2983
Fragments arranged by size:
2983 755 420
AccI GT'mk_AC
Cuts at: 0 2748 4158
Size: 2748 1410
Acc65I G'GTAC_C
Cuts at: 0 3322 4158
Size: 3322 836
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1649 1757 2159 3295 3743 4158
Size: 1649 108 402 1136 448 415
Fragments arranged by size:
1649 1136 448 415 402 108
AflIII A'CryG_T
Cuts at: 0 2619 2653 2751 4158
Size: 2619 34 98 1407
Fragments arranged by size:
2619 1407 98 34
AleI CACnn'nnGTG
Cuts at: 0 3109 4158
Size: 3109 1049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 881 4158
Size: 881 3277
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 270 302 940 972 4158
Size: 270 32 638 32 3186
Fragments arranged by size:
3186 638 270 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 112 196 778 1561 1940 3205 3529 3807
Size: 112 84 582 783 379 1265 324 278
Cuts at: 3807 4158
Size: 351
Fragments arranged by size:
1265 783 582 379 351 324 278 112
84
ApaI G_GGCC'C
Cuts at: 0 203 391 772 1415 3199 3268 3814 3900
Size: 203 188 381 643 1784 69 546 86
Cuts at: 3900 4128 4158
Size: 228 30
Fragments arranged by size:
1784 643 546 381 228 203 188 86
69 30
ApoI r'AATT_y
Cuts at: 0 1901 1928 1946 4158
Size: 1901 27 18 2212
Fragments arranged by size:
2212 1901 27 18
AvaI C'yCGr_G
Cuts at: 0 49 137 320 588 1112 1358 2421 2444
Size: 49 88 183 268 524 246 1063 23
Cuts at: 2444 2507 2540 3911 4158
Size: 63 33 1371 247
Fragments arranged by size:
1371 1063 524 268 247 246 183 88
63 49 33 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3554 3587 4158
Size: 3554 33 571
Fragments arranged by size:
3554 571 33
BamHI G'GATC_C
Cuts at: 0 186 278 2480 3255 3365 4158
Size: 186 92 2202 775 110 793
Fragments arranged by size:
2202 793 775 186 110 92
BanI G'GyrC_C
Cuts at: 0 85 1719 2895 3322 4158
Size: 85 1634 1176 427 836
Fragments arranged by size:
1634 1176 836 427 85
BanII G_rGCy'C
Cuts at: 0 15 154 178 203 236 391 485 595
Size: 15 139 24 25 33 155 94 110
Cuts at: 595 772 923 1328 1415 1853 2421 2603 3148
Size: 177 151 405 87 438 568 182 545
Cuts at: 3148 3199 3268 3404 3814 3900 3919 4128 4137
Size: 51 69 136 410 86 19 209 9
Cuts at: 4137 4158
Size: 21
Fragments arranged by size:
568 545 438 410 405 209 182 177
155 151 139 136 110 94 87 86
69 51 33 25 24 21 19 15
9
BbsI GAAGACnn'nnnn_
Cuts at: 0 3749 4158
Size: 3749 409
BbvCI CC'TCA_GC
Cuts at: 0 2108 4158
Size: 2108 2050
BclI T'GATC_A
Cuts at: 0 2919 4158
Size: 2919 1239
BglI GCCn_nnn'nGGC
Cuts at: 0 2314 2738 4132 4158
Size: 2314 424 1394 26
Fragments arranged by size:
2314 1394 424 26
BlpI GC'TnA_GC
Cuts at: 0 216 418 1487 3997 4158
Size: 216 202 1069 2510 161
Fragments arranged by size:
2510 1069 216 202 161
Bme1580I G_kGCm'C
Cuts at: 0 203 391 439 772 1302 1415 1916 2661
Size: 203 188 48 333 530 113 501 745
Cuts at: 2661 3116 3199 3268 3814 3900 4128 4158
Size: 455 83 69 546 86 228 30
Fragments arranged by size:
745 546 530 501 455 333 228 203
188 113 86 83 69 48 30
BmgBI CAC'GTC
Cuts at: 0 1839 1911 3938 4158
Size: 1839 72 2027 220
Fragments arranged by size:
2027 1839 220 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 768 788 2036 2170 3682 4158
Size: 768 20 1248 134 1512 476
Fragments arranged by size:
1512 1248 768 476 134 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 207 328 508 862 1075 1156 1201
Size: 191 16 121 180 354 213 81 45
Cuts at: 1201 1517 1600 1825 2099 2392 2831 2875 3041
Size: 316 83 225 274 293 439 44 166
Cuts at: 3041 3816 4158
Size: 775 342
Fragments arranged by size:
775 439 354 342 316 293 274 225
213 191 180 166 121 83 81 45
44 16
Bpu10I CC'TnA_GC
Cuts at: 0 163 1093 2108 2768 2816 2966 3017 3729
Size: 163 930 1015 660 48 150 51 712
Cuts at: 3729 4158
Size: 429
Fragments arranged by size:
1015 930 712 660 429 163 150 51
48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 706 4158
Size: 285 421 3452
Fragments arranged by size:
3452 421 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 1109 1352 4158
Size: 1109 243 2806
Fragments arranged by size:
2806 1109 243
BsaAI yAC'GTr
Cuts at: 0 2622 4026 4158
Size: 2622 1404 132
Fragments arranged by size:
2622 1404 132
BsaBI GATnn'nnATC
Cuts at: 0 2381 4158
Size: 2381 1777
BsaHI Gr'CG_yC
Cuts at: 0 2440 3769 4158
Size: 2440 1329 389
Fragments arranged by size:
2440 1329 389
BsaWI w'CCGG_w
Cuts at: 0 270 910 2300 3543 4067 4158
Size: 270 640 1390 1243 524 91
Fragments arranged by size:
1390 1243 640 524 270 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 604 607 634 940 970 1154 1184
Size: 577 27 3 27 306 30 184 30
Cuts at: 1184 1231 1261 4158
Size: 47 30 2897
Fragments arranged by size:
2897 577 306 184 47 30 30 30
27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 558 977 1340 1795 1798 1801 2212 3665
Size: 558 419 363 455 3 3 411 1453
Cuts at: 3665 4158
Size: 493
Fragments arranged by size:
1453 558 493 455 419 411 363 3
3
BseYI C'CCAG_C
Cuts at: 0 662 897 1759 1879 1933 2558 3369 3962
Size: 662 235 862 120 54 625 811 593
Cuts at: 3962 3968 4158
Size: 6 190
Fragments arranged by size:
862 811 662 625 593 235 190 120
54 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 907 1547 2869 3323 3515 4158
Size: 559 348 640 1322 454 192 643
Fragments arranged by size:
1322 643 640 559 454 348 192
BsiHKAI G_wGCw'C
Cuts at: 0 154 169 485 595 923 3404 4158
Size: 154 15 316 110 328 2481 754
Fragments arranged by size:
2481 754 328 316 154 110 15
BsmI GAATG_Cn'
Cuts at: 0 716 1883 2278 4158
Size: 716 1167 395 1880
Fragments arranged by size:
1880 1167 716 395
Bsp1286I G_dGCh'C
Cuts at: 0 15 154 169 178 203 236 391 439
Size: 15 139 15 9 25 33 155 48
Cuts at: 439 485 595 772 923 1302 1328 1415 1853
Size: 46 110 177 151 379 26 87 438
Cuts at: 1853 1916 2421 2603 2661 3116 3148 3199 3268
Size: 63 505 182 58 455 32 51 69
Cuts at: 3268 3404 3814 3900 3919 4128 4137 4158
Size: 136 410 86 19 209 9 21
Fragments arranged by size:
505 455 438 410 379 209 182 177
155 151 139 136 110 87 86 69
63 58 51 48 46 33 32 26
25 21 19 15 15 9 9
BspEI T'CCGG_A
Cuts at: 0 2300 3543 4158
Size: 2300 1243 615
Fragments arranged by size:
2300 1243 615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 420 1175 4158
Size: 420 755 2983
Fragments arranged by size:
2983 755 420
BsrBI CCG'CTC
Cuts at: 0 1002 2285 2405 2478 4158
Size: 1002 1283 120 73 1680
Fragments arranged by size:
1680 1283 1002 120 73
BsrDI GCAATG_nn'
Cuts at: 0 1862 4158
Size: 1862 2296
BsrFI r'CCGG_y
Cuts at: 0 1449 2534 2898 4158
Size: 1449 1085 364 1260
Fragments arranged by size:
1449 1260 1085 364
BssHII G'CGCG_C
Cuts at: 0 871 4158
Size: 871 3287
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1889 4158
Size: 1889 2269
BstEII G'GTnAC_C
Cuts at: 0 3127 4158
Size: 3127 1031
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1723 3840 4158
Size: 1723 2117 318
Fragments arranged by size:
2117 1723 318
BstYI r'GATC_y
Cuts at: 0 186 278 1668 2382 2480 3255 3365 4158
Size: 186 92 1390 714 98 775 110 793
Fragments arranged by size:
1390 793 775 714 186 110 98 92
Bsu36I CC'TnA_GG
Cuts at: 0 5 1571 4158
Size: 5 1566 2587
Fragments arranged by size:
2587 1566 5
BtgI C'CryG_G
Cuts at: 0 125 3701 4158
Size: 125 3576 457
Fragments arranged by size:
3576 457 125
BtsI GCAGTG_nn'
Cuts at: 0 2359 4158
Size: 2359 1799
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3371 3406 4158
Size: 3371 35 752
Fragments arranged by size:
3371 752 35
DraIII CAC_nnn'GTG
Cuts at: 0 92 4158
Size: 92 4066
DrdI GACnn_nn'nnGTC
Cuts at: 0 406 1465 3466 4158
Size: 406 1059 2001 692
Fragments arranged by size:
2001 1059 692 406
EaeI y'GGCC_r
Cuts at: 0 57 1037 2341 2739 4048 4158
Size: 57 980 1304 398 1309 110
Fragments arranged by size:
1309 1304 980 398 110 57
EarI CTCTTCn'nnn_
Cuts at: 0 57 1015 1184 1189 1231 1772 1776 1787
Size: 57 958 169 5 42 541 4 11
Cuts at: 1787 3595 4158
Size: 1808 563
Fragments arranged by size:
1808 958 563 541 169 57 42 11
5 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 617 778 983 2515 4158
Size: 244 373 161 205 1532 1643
Fragments arranged by size:
1643 1532 373 244 205 161
EcoICRI GAG'CTC
Cuts at: 0 152 483 593 921 3402 4158
Size: 152 331 110 328 2481 756
Fragments arranged by size:
2481 756 331 328 152 110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 207 328 508 862 1075 1156 1201
Size: 191 16 121 180 354 213 81 45
Cuts at: 1201 1517 1600 1649 1757 1825 2099 2159 2392
Size: 316 83 49 108 68 274 60 233
Cuts at: 2392 2831 2875 3041 3295 3743 3816 4158
Size: 439 44 166 254 448 73 342
Fragments arranged by size:
448 439 354 342 316 274 254 233
213 191 180 166 121 108 83 81
73 68 60 49 45 44 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 351 1171 2177 2819 3647 3890 4158
Size: 351 820 1006 642 828 243 268
Fragments arranged by size:
1006 828 820 642 351 268 243
EcoO109I rG'GnC_Cy
Cuts at: 0 244 357 388 408 768 1412 1458 1575
Size: 244 113 31 20 360 644 46 117
Cuts at: 1575 1616 1822 2089 2680 2878 3195 3264 3672
Size: 41 206 267 591 198 317 69 408
Cuts at: 3672 3811 3883 3896 3897 4124 4158
Size: 139 72 13 1 227 34
Fragments arranged by size:
644 591 408 360 317 267 244 227
206 198 139 117 113 72 69 46
41 34 31 20 13 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1294 4158
Size: 1294 2864
HaeII r_GCGC'y
Cuts at: 0 515 657 1224 2309 4158
Size: 515 142 567 1085 1849
Fragments arranged by size:
1849 1085 567 515 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 11 43 905 937 1092 1124 1154 1186
Size: 11 32 862 32 155 32 30 32
Cuts at: 1186 1197 1229 3759 3791 4158
Size: 11 32 2530 32 367
Fragments arranged by size:
2530 862 367 155 32 32 32 32
32 32 30 11 11
HincII GTy'rAC
Cuts at: 0 3217 4017 4158
Size: 3217 800 141
Fragments arranged by size:
3217 800 141
HpaI GTT'AAC
Cuts at: 0 4017 4158
Size: 4017 141
KpnI G_GTAC'C
Cuts at: 0 3326 4158
Size: 3326 832
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 3572 3731 4158
Size: 926 2646 159 427
Fragments arranged by size:
2646 926 427 159
MscI TGG'CCA
Cuts at: 0 1039 2741 4158
Size: 1039 1702 1417
Fragments arranged by size:
1702 1417 1039
MslI CAynn'nnrTG
Cuts at: 0 2652 3109 4024 4158
Size: 2652 457 915 134
Fragments arranged by size:
2652 915 457 134
MspA1I CmG'CkG
Cuts at: 0 823 891 1870 1940 2107 2334 2399 2496
Size: 823 68 979 70 167 227 65 97
Cuts at: 2496 2800 3526 3628 3823 4158
Size: 304 726 102 195 335
Fragments arranged by size:
979 823 726 335 304 227 195 167
102 97 70 68 65
NaeI GCC'GGC
Cuts at: 0 2536 2900 4158
Size: 2536 364 1258
Fragments arranged by size:
2536 1258 364
NcoI C'CATG_G
Cuts at: 0 125 3701 4158
Size: 125 3576 457
Fragments arranged by size:
3576 457 125
NgoMIV G'CCGG_C
Cuts at: 0 2534 2898 4158
Size: 2534 364 1260
Fragments arranged by size:
2534 1260 364
NruI TCG'CGA
Cuts at: 0 788 4158
Size: 788 3370
NspI r_CATG'y
Cuts at: 0 868 2657 2755 2839 4158
Size: 868 1789 98 84 1319
Fragments arranged by size:
1789 1319 868 98 84
PasI CC'CwG_GG
Cuts at: 0 196 1381 2676 3261 4128 4158
Size: 196 1185 1295 585 867 30
Fragments arranged by size:
1295 1185 867 585 196 30
PciI A'CATG_T
Cuts at: 0 2653 2751 4158
Size: 2653 98 1407
Fragments arranged by size:
2653 1407 98
PflMI CCAn_nnn'nTGG
Cuts at: 0 196 778 1408 1842 3867 4158
Size: 196 582 630 434 2025 291
Fragments arranged by size:
2025 630 582 434 291 196
PfoI T'CCnGG_A
Cuts at: 0 485 608 878 1637 1841 2369 4158
Size: 485 123 270 759 204 528 1789
Fragments arranged by size:
1789 759 528 485 270 204 123
PmlI CAC'GTG
Cuts at: 0 2622 4026 4158
Size: 2622 1404 132
Fragments arranged by size:
2622 1404 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 940 972 4158
Size: 940 32 3186
Fragments arranged by size:
3186 940 32
PpuMI rG'GwC_Cy
Cuts at: 0 357 408 1458 1575 1616 2680 4158
Size: 357 51 1050 117 41 1064 1478
Fragments arranged by size:
1478 1064 1050 357 117 51 41
PspOMI G'GGCC_C
Cuts at: 0 199 387 768 1411 3195 3264 3810 3896
Size: 199 188 381 643 1784 69 546 86
Cuts at: 3896 4124 4158
Size: 228 34
Fragments arranged by size:
1784 643 546 381 228 199 188 86
69 34
PstI C_TGCA'G
Cuts at: 0 117 650 689 743 2099 3214 4158
Size: 117 533 39 54 1356 1115 944
Fragments arranged by size:
1356 1115 944 533 117 54 39
PvuII CAG'CTG
Cuts at: 0 1870 2107 2334 2800 3628 3823 4158
Size: 1870 237 227 466 828 195 335
Fragments arranged by size:
1870 828 466 335 237 227 195
SacI G_AGCT'C
Cuts at: 0 154 485 595 923 3404 4158
Size: 154 331 110 328 2481 754
Fragments arranged by size:
2481 754 331 328 154 110
SapI GCTCTTCn'nnn_
Cuts at: 0 1015 3595 4158
Size: 1015 2580 563
Fragments arranged by size:
2580 1015 563
SfcI C'TryA_G
Cuts at: 0 113 646 685 739 2095 3210 4158
Size: 113 533 39 54 1356 1115 948
Fragments arranged by size:
1356 1115 948 533 113 54 39
SmaI CCC'GGG
Cuts at: 0 322 3913 4158
Size: 322 3591 245
Fragments arranged by size:
3591 322 245
SmlI C'TyrA_G
Cuts at: 0 300 721 1358 4158
Size: 300 421 637 2800
Fragments arranged by size:
2800 637 421 300
SphI G_CATG'C
Cuts at: 0 868 4158
Size: 868 3290
StuI AGG'CCT
Cuts at: 0 1661 4158
Size: 1661 2497
StyI C'CwwG_G
Cuts at: 0 15 125 1259 2085 3068 3701 4055 4158
Size: 15 110 1134 826 983 633 354 103
Fragments arranged by size:
1134 983 826 633 354 110 103 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 889 1699 2263 2377 2945 3504 3696 4158
Size: 889 810 564 114 568 559 192 462
Fragments arranged by size:
889 810 568 564 559 462 192 114
TatI w'GTAC_w
Cuts at: 0 572 1058 4158
Size: 572 486 3100
Fragments arranged by size:
3100 572 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1231 1263 1823 1855 2351 2383 3402 3434
Size: 1231 32 560 32 496 32 1019 32
Cuts at: 3434 4158
Size: 724
Fragments arranged by size:
1231 1019 724 560 496 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2439 3205 4158
Size: 2439 766 953
Fragments arranged by size:
2439 953 766
XbaI T'CTAG_A
Cuts at: 0 3660 4158
Size: 3660 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 638 4158
Size: 638 3520
XhoI C'TCGA_G
Cuts at: 0 1358 4158
Size: 1358 2800
XmaI C'CCGG_G
Cuts at: 0 320 3911 4158
Size: 320 3591 247
Fragments arranged by size:
3591 320 247
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BclI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HpaI KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NruI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SapI SfcI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI ApaLI AscI
AseI AsiSI AvrII BbeI BcgI BciVI BfrBI BglII
BmtI BplI BsiEI BsiWI BsmBI BspHI BsrGI BssSI
BstBI BstZ17I BtgZI ClaI DraI EagI EcoRI EcoRV
FseI FspI FspAI HindIII KasI MfeI MluI NarI
NdeI NheI NotI NsiI PacI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XmnI ZraI