Restriction map for
pF1KF0040
(Linear) (Six-base) MAPSORT of: FLJ00040.seq Check: 4592 from: 1 to: 3150
FLJ00040.orf
With 182 enzymes: SgfI *
March 2, 2010 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2299 3150
Size: 2299 851
AccI GT'mk_AC
Cuts at: 0 145 2639 3150
Size: 145 2494 511
Fragments arranged by size:
2494 511 145
Acc65I G'GTAC_C
Cuts at: 0 2685 3150
Size: 2685 465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 996 1810 2311 2662 3150
Size: 730 266 814 501 351 488
Fragments arranged by size:
814 730 501 488 351 266
AfeI AGC'GCT
Cuts at: 0 1909 2537 3150
Size: 1909 628 613
Fragments arranged by size:
1909 628 613
AflII C'TTAA_G
Cuts at: 0 1147 3150
Size: 1147 2003
AhdI GACnn_n'nnGTC
Cuts at: 0 1659 3150
Size: 1659 1491
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1750 1782 2588 2620 3150
Size: 1750 32 806 32 530
Fragments arranged by size:
1750 806 530 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 567 2032 2613 3002 3150
Size: 567 1465 581 389 148
Fragments arranged by size:
1465 581 567 389 148
ApaI G_GGCC'C
Cuts at: 0 3097 3150
Size: 3097 53
ApoI r'AATT_y
Cuts at: 0 656 990 2125 2655 3150
Size: 656 334 1135 530 495
Fragments arranged by size:
1135 656 530 495 334
BanI G'GyrC_C
Cuts at: 0 381 1456 2311 2685 3150
Size: 381 1075 855 374 465
Fragments arranged by size:
1075 855 465 381 374
BanII G_rGCy'C
Cuts at: 0 3097 3150
Size: 3097 53
BbeI G_GCGC'C
Cuts at: 0 1460 3150
Size: 1460 1690
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 256 444 2559 3150
Size: 23 233 188 2115 591
Fragments arranged by size:
2115 591 233 188 23
BciVI GTATCCnnnnn_n'
Cuts at: 0 1039 3150
Size: 1039 2111
BglI GCCn_nnn'nGGC
Cuts at: 0 2228 2360 2925 3012 3150
Size: 2228 132 565 87 138
Fragments arranged by size:
2228 565 138 132 87
BglII A'GATC_T
Cuts at: 0 741 2040 3150
Size: 741 1299 1110
Fragments arranged by size:
1299 1110 741
BlpI GC'TnA_GC
Cuts at: 0 815 3150
Size: 815 2335
Bme1580I G_kGCm'C
Cuts at: 0 278 1391 2152 2163 3097 3150
Size: 278 1113 761 11 934 53
Fragments arranged by size:
1113 934 761 278 53 11
BmgBI CAC'GTC
Cuts at: 0 1656 2565 3150
Size: 1656 909 585
Fragments arranged by size:
1656 909 585
BmrI ACTGGGnnnn_n'
Cuts at: 0 1051 3150
Size: 1051 2099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 593 2990 3150
Size: 593 2397 160
Fragments arranged by size:
2397 593 160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 931 1356 2107 3150
Size: 541 390 425 751 1043
Fragments arranged by size:
1043 751 541 425 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1789 1956 3150
Size: 1789 167 1194
Fragments arranged by size:
1789 1194 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 907 1978 3150
Size: 907 1071 1172
Fragments arranged by size:
1172 1071 907
BsaAI yAC'GTr
Cuts at: 0 681 3150
Size: 681 2469
BsaBI GATnn'nnATC
Cuts at: 0 2039 3150
Size: 2039 1111
BsaHI Gr'CG_yC
Cuts at: 0 1457 1736 1798 3150
Size: 1457 279 62 1352
Fragments arranged by size:
1457 1352 279 62
BsaWI w'CCGG_w
Cuts at: 0 771 2455 3150
Size: 771 1684 695
Fragments arranged by size:
1684 771 695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 873 903 904 934 1170 1200 2218 2248
Size: 873 30 1 30 236 30 1018 30
Cuts at: 2248 2437 2467 2485 2515 2588 2618 2916 2946
Size: 189 30 18 30 73 30 298 30
Cuts at: 2946 3150
Size: 204
Fragments arranged by size:
1018 873 298 236 204 189 73 30
30 30 30 30 30 30 30 18
1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1881 1944 1996 2092 2223 3150
Size: 1881 63 52 96 131 927
Fragments arranged by size:
1881 927 131 96 63 52
BseYI C'CCAG_C
Cuts at: 0 1721 2163 3150
Size: 1721 442 987
Fragments arranged by size:
1721 987 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 892 1531 1591 1593 1686 1915 2132
Size: 93 799 639 60 2 93 229 217
Cuts at: 2132 2242 2524 3150
Size: 110 282 626
Fragments arranged by size:
799 639 626 282 229 217 110 93
93 60 2
BsiEI CG_ry'CG
Cuts at: 0 2265 3083 3150
Size: 2265 818 67
Fragments arranged by size:
2265 818 67
BsmI GAATG_Cn'
Cuts at: 0 499 540 2403 3150
Size: 499 41 1863 747
Fragments arranged by size:
1863 747 499 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1190 1335 1370 1789 3150
Size: 1190 145 35 419 1361
Fragments arranged by size:
1361 1190 419 145 35
Bsp1286I G_dGCh'C
Cuts at: 0 278 1391 2152 2163 3097 3150
Size: 278 1113 761 11 934 53
Fragments arranged by size:
1113 934 761 278 53 11
BspHI T'CATG_A
Cuts at: 0 651 3150
Size: 651 2499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2299 2963 3150
Size: 2299 664 187
Fragments arranged by size:
2299 664 187
BsrBI CCG'CTC
Cuts at: 0 1497 2340 3150
Size: 1497 843 810
Fragments arranged by size:
1497 843 810
BsrDI GCAATG_nn'
Cuts at: 0 1211 1689 1709 1747 3150
Size: 1211 478 20 38 1403
Fragments arranged by size:
1403 1211 478 38 20
BsrFI r'CCGG_y
Cuts at: 0 1966 3150
Size: 1966 1184
BssSI C'ACGA_G
Cuts at: 0 794 1621 2467 2542 3150
Size: 794 827 846 75 608
Fragments arranged by size:
846 827 794 608 75
BstBI TT'CG_AA
Cuts at: 0 1064 2395 3150
Size: 1064 1331 755
Fragments arranged by size:
1331 1064 755
BstEII G'GTnAC_C
Cuts at: 0 1222 3150
Size: 1222 1928
BstXI CCAn_nnnn'nTGG
Cuts at: 0 94 2833 3150
Size: 94 2739 317
Fragments arranged by size:
2739 317 94
BstYI r'GATC_y
Cuts at: 0 390 741 971 1775 2040 2971 3150
Size: 390 351 230 804 265 931 179
Fragments arranged by size:
931 804 390 351 265 230 179
BtgI C'CryG_G
Cuts at: 0 93 689 1460 1482 1913 2592 3150
Size: 93 596 771 22 431 679 558
Fragments arranged by size:
771 679 596 558 431 93 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 1325 3150
Size: 450 875 1825
Fragments arranged by size:
1825 875 450
BtsI GCAGTG_nn'
Cuts at: 0 638 2479 3150
Size: 638 1841 671
Fragments arranged by size:
1841 671 638
DraI TTT'AAA
Cuts at: 0 675 3150
Size: 675 2475
DrdI GACnn_nn'nnGTC
Cuts at: 0 2332 3150
Size: 2332 818
EaeI y'GGCC_r
Cuts at: 0 1617 2262 2460 3004 3150
Size: 1617 645 198 544 146
Fragments arranged by size:
1617 645 544 198 146
EagI C'GGCC_G
Cuts at: 0 2262 3150
Size: 2262 888
EarI CTCTTCn'nnn_
Cuts at: 0 179 308 365 2167 3150
Size: 179 129 57 1802 983
Fragments arranged by size:
1802 983 179 129 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2269 2284 3150
Size: 2269 15 866
Fragments arranged by size:
2269 866 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 541 730 931 996 1356 1810 2107 2311
Size: 541 189 201 65 360 454 297 204
Cuts at: 2311 2662 3150
Size: 351 488
Fragments arranged by size:
541 488 454 360 351 297 204 201
189 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1863 3150
Size: 1863 1287
EcoO109I rG'GnC_Cy
Cuts at: 0 1887 2082 2418 2498 3093 3150
Size: 1887 195 336 80 595 57
Fragments arranged by size:
1887 595 336 195 80 57
EcoRI G'AATT_C
Cuts at: 0 2125 2655 3150
Size: 2125 530 495
Fragments arranged by size:
2125 530 495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 2583 3150
Size: 234 2349 567
Fragments arranged by size:
2349 567 234
HaeII r_GCGC'y
Cuts at: 0 1460 1911 2539 3150
Size: 1460 451 628 611
Fragments arranged by size:
1460 628 611 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 530 922 954 1170 1202 1645 1677
Size: 498 32 392 32 216 32 443 32
Cuts at: 1677 2218 2250 3150
Size: 541 32 900
Fragments arranged by size:
900 541 498 443 392 216 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 847 3150
Size: 847 2303
KasI G'GCGC_C
Cuts at: 0 1456 3150
Size: 1456 1694
KpnI G_GTAC'C
Cuts at: 0 2689 3150
Size: 2689 461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2045 3150
Size: 2045 1105
MscI TGG'CCA
Cuts at: 0 2462 3006 3150
Size: 2462 544 144
Fragments arranged by size:
2462 544 144
MspA1I CmG'CkG
Cuts at: 0 819 1721 2241 2833 3150
Size: 819 902 520 592 317
Fragments arranged by size:
902 819 592 520 317
NaeI GCC'GGC
Cuts at: 0 1968 3150
Size: 1968 1182
NarI GG'CG_CC
Cuts at: 0 1457 3150
Size: 1457 1693
NcoI C'CATG_G
Cuts at: 0 93 689 1460 1482 3150
Size: 93 596 771 22 1668
Fragments arranged by size:
1668 771 596 93 22
NgoMIV G'CCGG_C
Cuts at: 0 1966 3150
Size: 1966 1184
NspI r_CATG'y
Cuts at: 0 2248 3150
Size: 2248 902
PasI CC'CwG_GG
Cuts at: 0 3090 3150
Size: 3090 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 348 3150
Size: 348 2802
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 348 380 454 486 2588 2620 3150
Size: 348 32 74 32 2102 32 530
Fragments arranged by size:
2102 530 348 74 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 2082 2418 3150
Size: 2082 336 732
Fragments arranged by size:
2082 732 336
PspOMI G'GGCC_C
Cuts at: 0 3093 3150
Size: 3093 57
PstI C_TGCA'G
Cuts at: 0 1757 3150
Size: 1757 1393
PvuII CAG'CTG
Cuts at: 0 819 2833 3150
Size: 819 2014 317
Fragments arranged by size:
2014 819 317
SapI GCTCTTCn'nnn_
Cuts at: 0 179 365 3150
Size: 179 186 2785
Fragments arranged by size:
2785 186 179
SfcI C'TryA_G
Cuts at: 0 1753 1998 2488 2636 3150
Size: 1753 245 490 148 514
Fragments arranged by size:
1753 514 490 245 148
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3012 3150
Size: 3012 138
SfoI GGC'GCC
Cuts at: 0 1458 3150
Size: 1458 1692
SmlI C'TyrA_G
Cuts at: 0 1147 1804 1971 3150
Size: 1147 657 167 1179
Fragments arranged by size:
1179 1147 657 167
StuI AGG'CCT
Cuts at: 0 3015 3150
Size: 3015 135
StyI C'CwwG_G
Cuts at: 0 93 689 1451 1460 1482 1731 3150
Size: 93 596 762 9 22 249 1419
Fragments arranged by size:
1419 762 596 249 93 22 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 904 936 2485 2517 2916 2948 3150
Size: 904 32 1549 32 399 32 202
Fragments arranged by size:
1549 904 399 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1182 2104 3150
Size: 1182 922 1046
Fragments arranged by size:
1182 1046 922
XbaI T'CTAG_A
Cuts at: 0 2043 3150
Size: 2043 1107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 807 1458 3150
Size: 807 651 1692
Fragments arranged by size:
1692 807 651
XmnI GAAnn'nnTTC
Cuts at: 0 1082 2675 2853 3150
Size: 1082 1593 178 297
Fragments arranged by size:
1593 1082 297 178
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AhdI AloI
AlwNI ApaI ApoI BanI BanII BbeI BbsI BciVI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII KasI KpnI MmeI MscI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PflMI PpiI PpuMI PspOMI
PstI PvuII SapI SfcI SfiI SfoI SmlI StuI
StyI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AclI AflIII AgeI AleI AlfI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BcgI
BclI BfrBI BmtI Bpu10I BsiHKAI BsiWI BspEI BsrGI
BssHII BstAPI BstZ17I Bsu36I ClaI CspCI DraIII EcoICRI
EcoRV FseI FspI FspAI HincII HpaI MfeI MluI
MslI NdeI NheI NotI NruI NsiI PacI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII TatI XhoI XmaI ZraI