Restriction map for
pF1KSDF0044
(Linear) (Six-base) MAPSORT of: FLJ00044.seq Check: 1588 from: 1 to: 2967
FLJ00044.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 141 323 780 1079 1210 1527 2016 2967
Size: 141 182 457 299 131 317 489 951
Fragments arranged by size:
951 489 457 317 299 182 141 131
AatII G_ACGT'C
Cuts at: 0 1109 2967
Size: 1109 1858
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1566 2967
Size: 1566 1401
AfeI AGC'GCT
Cuts at: 0 661 2967
Size: 661 2306
AleI CACnn'nnGTG
Cuts at: 0 454 640 1631 2161 2967
Size: 454 186 991 530 806
Fragments arranged by size:
991 806 530 454 186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1649 2967
Size: 1649 1318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 1588 1620 2131 2163 2967
Size: 148 32 1408 32 511 32 804
Fragments arranged by size:
1408 804 511 148 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 804 840 930 1494 1542 2745 2967
Size: 804 36 90 564 48 1203 222
Fragments arranged by size:
1203 804 564 222 90 48 36
ApaI G_GGCC'C
Cuts at: 0 1017 1440 2683 2967
Size: 1017 423 1243 284
Fragments arranged by size:
1243 1017 423 284
ApoI r'AATT_y
Cuts at: 0 2830 2967
Size: 2830 137
AvaI C'yCGr_G
Cuts at: 0 46 94 170 287 403 1327 1366 2846
Size: 46 48 76 117 116 924 39 1480
Cuts at: 2846 2967
Size: 121
Fragments arranged by size:
1480 924 121 117 116 76 48 46
39
BanI G'GyrC_C
Cuts at: 0 174 291 712 761 1232 2194 2667 2716
Size: 174 117 421 49 471 962 473 49
Cuts at: 2716 2727 2865 2876 2967
Size: 11 138 11 91
Fragments arranged by size:
962 473 471 421 174 138 117 91
49 49 11 11
BanII G_rGCy'C
Cuts at: 0 185 404 691 1017 1042 1198 1375 1440
Size: 185 219 287 326 25 156 177 65
Cuts at: 1440 1897 2252 2585 2683 2967
Size: 457 355 333 98 284
Fragments arranged by size:
457 355 333 326 287 284 219 185
177 156 98 65 25
BbeI G_GCGC'C
Cuts at: 0 2671 2731 2869 2967
Size: 2671 60 138 98
Fragments arranged by size:
2671 138 98 60
BbvCI CC'TCA_GC
Cuts at: 0 112 2967
Size: 112 2855
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 299 2569 2603 2967
Size: 265 34 2270 34 364
Fragments arranged by size:
2270 364 265 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1449 2356 2725 2874 2967
Size: 1449 907 369 149 93
Fragments arranged by size:
1449 907 369 149 93
BlpI GC'TnA_GC
Cuts at: 0 656 2494 2967
Size: 656 1838 473
Fragments arranged by size:
1838 656 473
Bme1580I G_kGCm'C
Cuts at: 0 296 498 717 1017 1235 1367 1440 1622
Size: 296 202 219 300 218 132 73 182
Cuts at: 1622 1638 2197 2628 2683 2694 2879 2967
Size: 16 559 431 55 11 185 88
Fragments arranged by size:
559 431 300 296 219 218 202 185
182 132 88 73 55 16 11
BmgBI CAC'GTC
Cuts at: 0 452 2538 2967
Size: 452 2086 429
Fragments arranged by size:
2086 452 429
BmrI ACTGGGnnnn_n'
Cuts at: 0 1900 2722 2967
Size: 1900 822 245
Fragments arranged by size:
1900 822 245
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 591 2967
Size: 183 408 2376
Fragments arranged by size:
2376 408 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 416 1060 1171 1294 1309 1423 1525
Size: 304 112 644 111 123 15 114 102
Cuts at: 1525 1741 1760 1834 2032 2866 2967
Size: 216 19 74 198 834 101
Fragments arranged by size:
834 644 304 216 198 123 114 112
111 102 101 74 19 15
Bpu10I CC'TnA_GC
Cuts at: 0 112 179 364 1036 2967
Size: 112 67 185 672 1931
Fragments arranged by size:
1931 672 185 112 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 1810 1859 2845 2967
Size: 1810 49 986 122
Fragments arranged by size:
1810 986 122 49
BsaAI yAC'GTr
Cuts at: 0 501 1356 2967
Size: 501 855 1611
Fragments arranged by size:
1611 855 501
BsaBI GATnn'nnATC
Cuts at: 0 2128 2967
Size: 2128 839
BsaHI Gr'CG_yC
Cuts at: 0 749 1106 1949 2668 2728 2866 2967
Size: 749 357 843 719 60 138 101
Fragments arranged by size:
843 749 719 357 138 101 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 148 178 406 436 1438 1468 1501 1531
Size: 148 30 228 30 1002 30 33 30
Cuts at: 1531 1588 1618 2967
Size: 57 30 1349
Fragments arranged by size:
1349 1002 228 148 57 33 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 172 787 1306 1309 1498 1522 2260
Size: 64 108 615 519 3 189 24 738
Cuts at: 2260 2967
Size: 707
Fragments arranged by size:
738 707 615 519 189 108 64 24
3
BseYI C'CCAG_C
Cuts at: 0 137 252 684 1008 1147 1416 1422 1753
Size: 137 115 432 324 139 269 6 331
Cuts at: 1753 1998 2016 2967
Size: 245 18 951
Fragments arranged by size:
951 432 331 324 269 245 139 137
115 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 631 1555 1864 2967
Size: 295 336 924 309 1103
Fragments arranged by size:
1103 924 336 309 295
BsiEI CG_ry'CG
Cuts at: 0 1856 2390 2724 2967
Size: 1856 534 334 243
Fragments arranged by size:
1856 534 334 243
BsiHKAI G_wGCw'C
Cuts at: 0 281 2252 2441 2585 2967
Size: 281 1971 189 144 382
Fragments arranged by size:
1971 382 281 189 144
BsmI GAATG_Cn'
Cuts at: 0 524 884 2967
Size: 524 360 2083
Fragments arranged by size:
2083 524 360
BsmBI CGTCTCn'nnnn_
Cuts at: 0 562 2967
Size: 562 2405
Bsp1286I G_dGCh'C
Cuts at: 0 185 281 296 404 498 691 717 1017
Size: 185 96 15 108 94 193 26 300
Cuts at: 1017 1042 1198 1235 1367 1375 1440 1622 1638
Size: 25 156 37 132 8 65 182 16
Cuts at: 1638 1897 2197 2252 2441 2585 2628 2683 2694
Size: 259 300 55 189 144 43 55 11
Cuts at: 2694 2879 2967
Size: 185 88
Fragments arranged by size:
300 300 259 193 189 185 185 182
156 144 132 108 96 94 88 65
55 55 43 37 26 25 16 15
11 8
BspHI T'CATG_A
Cuts at: 0 914 1665 1772 2967
Size: 914 751 107 1195
Fragments arranged by size:
1195 914 751 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 141 323 780 1079 1210 1527 2016 2967
Size: 141 182 457 299 131 317 489 951
Fragments arranged by size:
951 489 457 317 299 182 141 131
BsrBI CCG'CTC
Cuts at: 0 162 326 368 1527 2454 2733 2967
Size: 162 164 42 1159 927 279 234
Fragments arranged by size:
1159 927 279 234 164 162 42
BsrFI r'CCGG_y
Cuts at: 0 89 730 989 2389 2573 2967
Size: 89 641 259 1400 184 394
Fragments arranged by size:
1400 641 394 259 184 89
BssHII G'CGCG_C
Cuts at: 0 898 2967
Size: 898 2069
BssSI C'ACGA_G
Cuts at: 0 2578 2967
Size: 2578 389
BstAPI GCAn_nnn'nTGC
Cuts at: 0 930 1542 1735 2031 2967
Size: 930 612 193 296 936
Fragments arranged by size:
936 930 612 296 193
BstEII G'GTnAC_C
Cuts at: 0 256 2967
Size: 256 2711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1761 1998 2712 2967
Size: 1761 237 714 255
Fragments arranged by size:
1761 714 255 237
BstYI r'GATC_y
Cuts at: 0 674 779 2967
Size: 674 105 2188
Fragments arranged by size:
2188 674 105
Bsu36I CC'TnA_GG
Cuts at: 0 2685 2967
Size: 2685 282
BtgI C'CryG_G
Cuts at: 0 11 38 303 332 1163 2442 2806 2967
Size: 11 27 265 29 831 1279 364 161
Fragments arranged by size:
1279 831 364 265 161 29 27 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 71 1989 2219 2302 2484 2967
Size: 71 1918 230 83 182 483
Fragments arranged by size:
1918 483 230 182 83 71
BtsI GCAGTG_nn'
Cuts at: 0 228 650 1044 1411 1654 2192 2287 2967
Size: 228 422 394 367 243 538 95 680
Fragments arranged by size:
680 538 422 394 367 243 228 95
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2088 2123 2967
Size: 2088 35 844
Fragments arranged by size:
2088 844 35
DraIII CAC_nnn'GTG
Cuts at: 0 223 504 543 2716 2967
Size: 223 281 39 2173 251
Fragments arranged by size:
2173 281 251 223 39
DrdI GACnn_nn'nnGTC
Cuts at: 0 2755 2967
Size: 2755 212
EaeI y'GGCC_r
Cuts at: 0 14 81 1806 1853 2387 2967
Size: 14 67 1725 47 534 580
Fragments arranged by size:
1725 580 534 67 47 14
EagI C'GGCC_G
Cuts at: 0 1853 2387 2967
Size: 1853 534 580
Fragments arranged by size:
1853 580 534
EarI CTCTTCn'nnn_
Cuts at: 0 490 1115 2419 2967
Size: 490 625 1304 548
Fragments arranged by size:
1304 625 548 490
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2079 2967
Size: 2079 888
EcoICRI GAG'CTC
Cuts at: 0 2250 2583 2967
Size: 2250 333 384
Fragments arranged by size:
2250 384 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 416 1060 1171 1294 1309 1423 1525
Size: 304 112 644 111 123 15 114 102
Cuts at: 1525 1566 1741 1760 1834 2032 2866 2967
Size: 41 175 19 74 198 834 101
Fragments arranged by size:
834 644 304 198 175 123 114 112
111 102 101 74 41 19 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 769 1444 1504 2839 2967
Size: 769 675 60 1335 128
Fragments arranged by size:
1335 769 675 128 60
EcoO109I rG'GnC_Cy
Cuts at: 0 43 1013 1014 1324 1437 1576 2295 2680
Size: 43 970 1 310 113 139 719 385
Cuts at: 2680 2967
Size: 287
Fragments arranged by size:
970 719 385 310 287 139 113 43
1
EcoRI G'AATT_C
Cuts at: 0 2830 2967
Size: 2830 137
FseI GG_CCGG'CC
Cuts at: 0 93 2967
Size: 93 2874
FspI TGC'GCA
Cuts at: 0 1477 1546 2967
Size: 1477 69 1421
Fragments arranged by size:
1477 1421 69
HaeII r_GCGC'y
Cuts at: 0 103 119 411 629 663 1270 1402 1979
Size: 103 16 292 218 34 607 132 577
Cuts at: 1979 2671 2731 2869 2967
Size: 692 60 138 98
Fragments arranged by size:
692 607 577 292 218 138 132 103
98 60 34 16
HincII GTy'rAC
Cuts at: 0 1850 2532 2967
Size: 1850 682 435
Fragments arranged by size:
1850 682 435
KasI G'GCGC_C
Cuts at: 0 2667 2727 2865 2967
Size: 2667 60 138 102
Fragments arranged by size:
2667 138 102 60
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1499 2967
Size: 914 585 1468
Fragments arranged by size:
1468 914 585
MscI TGG'CCA
Cuts at: 0 1808 2967
Size: 1808 1159
MslI CAynn'nnrTG
Cuts at: 0 454 640 1631 2161 2967
Size: 454 186 991 530 806
Fragments arranged by size:
991 806 530 454 186
MspA1I CmG'CkG
Cuts at: 0 13 40 305 597 840 1434 1491 1761
Size: 13 27 265 292 243 594 57 270
Cuts at: 1761 1793 2093 2226 2444 2654 2808 2967
Size: 32 300 133 218 210 154 159
Fragments arranged by size:
594 300 292 270 265 243 218 210
159 154 133 57 32 27 13
NaeI GCC'GGC
Cuts at: 0 91 732 991 2391 2575 2967
Size: 91 641 259 1400 184 392
Fragments arranged by size:
1400 641 392 259 184 91
NarI GG'CG_CC
Cuts at: 0 2668 2728 2866 2967
Size: 2668 60 138 101
Fragments arranged by size:
2668 138 101 60
NcoI C'CATG_G
Cuts at: 0 1163 2967
Size: 1163 1804
NgoMIV G'CCGG_C
Cuts at: 0 89 730 989 2389 2573 2967
Size: 89 641 259 1400 184 394
Fragments arranged by size:
1400 641 394 259 184 89
NspI r_CATG'y
Cuts at: 0 906 2967
Size: 906 2061
PasI CC'CwG_GG
Cuts at: 0 1375 2568 2967
Size: 1375 1193 399
Fragments arranged by size:
1375 1193 399
PflMI CCAn_nnn'nTGG
Cuts at: 0 504 543 758 2568 2745 2967
Size: 504 39 215 1810 177 222
Fragments arranged by size:
1810 504 222 215 177 39
PfoI T'CCnGG_A
Cuts at: 0 393 782 1271 1607 2744 2967
Size: 393 389 489 336 1137 223
Fragments arranged by size:
1137 489 393 389 336 223
PmlI CAC'GTG
Cuts at: 0 501 1356 2967
Size: 501 855 1611
Fragments arranged by size:
1611 855 501
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 1588 1620 1900 1932 2967
Size: 148 32 1408 32 280 32 1035
Fragments arranged by size:
1408 1035 280 148 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 43 1576 2295 2967
Size: 43 1533 719 672
Fragments arranged by size:
1533 719 672 43
PspOMI G'GGCC_C
Cuts at: 0 1013 1436 2679 2967
Size: 1013 423 1243 288
Fragments arranged by size:
1243 1013 423 288
PstI C_TGCA'G
Cuts at: 0 602 935 1157 1264 2027 2225 2967
Size: 602 333 222 107 763 198 742
Fragments arranged by size:
763 742 602 333 222 198 107
PvuII CAG'CTG
Cuts at: 0 597 840 1491 2093 2226 2967
Size: 597 243 651 602 133 741
Fragments arranged by size:
741 651 602 597 243 133
SacI G_AGCT'C
Cuts at: 0 2252 2585 2967
Size: 2252 333 382
Fragments arranged by size:
2252 382 333
SacII CC_GC'GG
Cuts at: 0 14 41 306 2445 2809 2967
Size: 14 27 265 2139 364 158
Fragments arranged by size:
2139 364 265 158 27 14
SanDI GG'GwC_CC
Cuts at: 0 43 1576 2967
Size: 43 1533 1391
Fragments arranged by size:
1533 1391 43
SbfI CC_TGCA'GG
Cuts at: 0 935 2967
Size: 935 2032
SexAI A'CCwGG_T
Cuts at: 0 539 2967
Size: 539 2428
SfcI C'TryA_G
Cuts at: 0 598 931 1153 1260 2023 2221 2967
Size: 598 333 222 107 763 198 746
Fragments arranged by size:
763 746 598 333 222 198 107
SfoI GGC'GCC
Cuts at: 0 2669 2729 2867 2967
Size: 2669 60 138 100
Fragments arranged by size:
2669 138 100 60
SgrAI Cr'CCGG_yG
Cuts at: 0 730 2967
Size: 730 2237
SmaI CCC'GGG
Cuts at: 0 172 289 1368 2848 2967
Size: 172 117 1079 1480 119
Fragments arranged by size:
1480 1079 172 119 117
SphI G_CATG'C
Cuts at: 0 906 2967
Size: 906 2061
StuI AGG'CCT
Cuts at: 0 1279 2967
Size: 1279 1688
StyI C'CwwG_G
Cuts at: 0 1163 1803 1859 2967
Size: 1163 640 56 1108
Fragments arranged by size:
1163 1108 640 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 934 1215 2704 2967
Size: 934 281 1489 263
Fragments arranged by size:
1489 934 281 263
TatI w'GTAC_w
Cuts at: 0 242 2967
Size: 242 2725
XmaI C'CCGG_G
Cuts at: 0 170 287 1366 2846 2967
Size: 170 117 1079 1480 121
Fragments arranged by size:
1480 1079 170 121 117
ZraI GAC'GTC
Cuts at: 0 1107 2967
Size: 1107 1860
Enzymes that do cut:
AarI AatII AcuI AfeI AleI AlfI AloI AlwNI
ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
FspI HaeII HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SanDI SbfI SexAI SfcI SfoI SgrAI SmaI SphI
StuI StyI TaqII TatI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AgeI AhdI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglII BmtI BpuEI BsaWI BsiWI BspEI
BsrDI BsrGI BstBI BstZ17I ClaI DraI EcoRV FalI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SalI SapI ScaI
SfiI SgfI SmlI SnaBI SpeI SrfI SspI SwaI
TstI Tth111I XbaI XcmI XhoI XmnI