Restriction map for
pF1KF0052
(Linear) (Six-base) MAPSORT of: FLJ00052.seq Check: 527 from: 1 to: 984
FLJ00052.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
Acc65I G'GTAC_C
Cuts at: 0 803 984
Size: 803 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 984
Size: 928 56
AflII C'TTAA_G
Cuts at: 0 676 984
Size: 676 308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 740 984
Size: 740 244
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 332 364 984
Size: 332 32 620
Fragments arranged by size:
620 332 32
AlwNI CAG_nnn'CTG
Cuts at: 0 852 984
Size: 852 132
ApoI r'AATT_y
Cuts at: 0 412 984
Size: 412 572
AvaI C'yCGr_G
Cuts at: 0 58 70 984
Size: 58 12 914
Fragments arranged by size:
914 58 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 199 232 984
Size: 199 33 752
Fragments arranged by size:
752 199 33
BanI G'GyrC_C
Cuts at: 0 803 984
Size: 803 181
BanII G_rGCy'C
Cuts at: 0 956 984
Size: 956 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 146 194 425 907 984
Size: 146 48 231 482 77
Fragments arranged by size:
482 231 146 77 48
BciVI GTATCCnnnnn_n'
Cuts at: 0 320 984
Size: 320 664
BclI T'GATC_A
Cuts at: 0 863 984
Size: 863 121
BglII A'GATC_T
Cuts at: 0 887 984
Size: 887 97
BlpI GC'TnA_GC
Cuts at: 0 455 975 984
Size: 455 520 9
Fragments arranged by size:
520 455 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 109 334 533 593 728 850 984
Size: 62 47 225 199 60 135 122 134
Fragments arranged by size:
225 199 135 134 122 62 60 47
Bpu10I CC'TnA_GC
Cuts at: 0 713 984
Size: 713 271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 984
Size: 223 761
BsaI GGTCTCn'nnnn_
Cuts at: 0 684 984
Size: 684 300
BsaBI GATnn'nnATC
Cuts at: 0 925 984
Size: 925 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 332 362 984
Size: 332 30 622
Fragments arranged by size:
622 332 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 609 984
Size: 88 521 375
Fragments arranged by size:
521 375 88
BseYI C'CCAG_C
Cuts at: 0 614 984
Size: 614 370
BsiHKAI G_wGCw'C
Cuts at: 0 719 984
Size: 719 265
BsmI GAATG_Cn'
Cuts at: 0 339 984
Size: 339 645
Bsp1286I G_dGCh'C
Cuts at: 0 719 956 984
Size: 719 237 28
Fragments arranged by size:
719 237 28
BspHI T'CATG_A
Cuts at: 0 539 984
Size: 539 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 612 984
Size: 612 372
BsrFI r'CCGG_y
Cuts at: 0 51 968 984
Size: 51 917 16
Fragments arranged by size:
917 51 16
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 984
Size: 258 726
BstBI TT'CG_AA
Cuts at: 0 758 984
Size: 758 226
BstXI CCAn_nnnn'nTGG
Cuts at: 0 828 984
Size: 828 156
BstYI r'GATC_y
Cuts at: 0 654 887 926 984
Size: 654 233 39 58
Fragments arranged by size:
654 233 58 39
BtgI C'CryG_G
Cuts at: 0 8 734 984
Size: 8 726 250
Fragments arranged by size:
726 250 8
BtsI GCAGTG_nn'
Cuts at: 0 410 984
Size: 410 574
EarI CTCTTCn'nnn_
Cuts at: 0 77 283 349 961 984
Size: 77 206 66 612 23
Fragments arranged by size:
612 206 77 66 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 955 984
Size: 955 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 109 334 533 593 728 850 928
Size: 62 47 225 199 60 135 122 78
Cuts at: 928 984
Size: 56
Fragments arranged by size:
225 199 135 122 78 62 60 56
47
HaeII r_GCGC'y
Cuts at: 0 49 98 975 984
Size: 49 49 877 9
Fragments arranged by size:
877 49 49 9
HincII GTy'rAC
Cuts at: 0 632 984
Size: 632 352
HindIII A'AGCT_T
Cuts at: 0 673 984
Size: 673 311
HpaI GTT'AAC
Cuts at: 0 632 984
Size: 632 352
KpnI G_GTAC'C
Cuts at: 0 807 984
Size: 807 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 984
Size: 113 871
MslI CAynn'nnrTG
Cuts at: 0 919 984
Size: 919 65
MspA1I CmG'CkG
Cuts at: 0 10 116 354 736 979 984
Size: 10 106 238 382 243 5
Fragments arranged by size:
382 243 238 106 10 5
NaeI GCC'GGC
Cuts at: 0 53 970 984
Size: 53 917 14
Fragments arranged by size:
917 53 14
NgoMIV G'CCGG_C
Cuts at: 0 51 968 984
Size: 51 917 16
Fragments arranged by size:
917 51 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 332 364 984
Size: 332 32 620
Fragments arranged by size:
620 332 32
PvuII CAG'CTG
Cuts at: 0 116 354 979 984
Size: 116 238 625 5
Fragments arranged by size:
625 238 116 5
SacII CC_GC'GG
Cuts at: 0 11 737 984
Size: 11 726 247
Fragments arranged by size:
726 247 11
SapI GCTCTTCn'nnn_
Cuts at: 0 77 961 984
Size: 77 884 23
Fragments arranged by size:
884 77 23
ScaI AGT'ACT
Cuts at: 0 64 984
Size: 64 920
SfcI C'TryA_G
Cuts at: 0 872 984
Size: 872 112
SgrAI Cr'CCGG_yG
Cuts at: 0 968 984
Size: 968 16
SmlI C'TyrA_G
Cuts at: 0 238 676 984
Size: 238 438 308
Fragments arranged by size:
438 308 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 699 882 984
Size: 699 183 102
Fragments arranged by size:
699 183 102
TatI w'GTAC_w
Cuts at: 0 62 314 785 984
Size: 62 252 471 199
Fragments arranged by size:
471 252 199 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 891 923 984
Size: 891 32 61
Fragments arranged by size:
891 61 32
Enzymes that do cut:
Acc65I AcuI AflII AlfI AloI AlwNI ApoI AvaI
BaeI BanI BanII BbsI BciVI BclI BglII BlpI
BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BstAPI BstBI
BstXI BstYI BtgI BtsI EarI EciI Eco57MI HaeII
HincII HindIII HpaI KpnI MmeI MslI MspA1I NaeI
NgoMIV PpiI PvuII SacII SapI ScaI SfcI SgrAI
SmlI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI ApaI ApaLI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BfrBI BglI Bme1580I BmgBI BmrI
BmtI BplI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BssSI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I KasI MfeI MluI MscI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SalI SanDI SbfI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI