Restriction map for
pF1KSDF0070
(Linear) (Six-base) MAPSORT of: FLJ00070.seq Check: 5509 from: 1 to: 3906
FLJ00070.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 779 3906
Size: 779 3127
AccI GT'mk_AC
Cuts at: 0 2879 3906
Size: 2879 1027
Acc65I G'GTAC_C
Cuts at: 0 445 3906
Size: 445 3461
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2638 3906
Size: 2638 1268
AfeI AGC'GCT
Cuts at: 0 178 1321 3811 3906
Size: 178 1143 2490 95
Fragments arranged by size:
2490 1143 178 95
AflIII A'CryG_T
Cuts at: 0 2375 3404 3906
Size: 2375 1029 502
Fragments arranged by size:
2375 1029 502
AgeI A'CCGG_T
Cuts at: 0 1545 1811 3906
Size: 1545 266 2095
Fragments arranged by size:
2095 1545 266
AleI CACnn'nnGTG
Cuts at: 0 973 2714 2803 3906
Size: 973 1741 89 1103
Fragments arranged by size:
1741 1103 973 89
AlwNI CAG_nnn'CTG
Cuts at: 0 384 771 991 1720 2255 2906 3222 3906
Size: 384 387 220 729 535 651 316 684
Fragments arranged by size:
729 684 651 535 387 384 316 220
ApaI G_GGCC'C
Cuts at: 0 11 486 587 1777 3344 3906
Size: 11 475 101 1190 1567 562
Fragments arranged by size:
1567 1190 562 475 101 11
ApaLI G'TGCA_C
Cuts at: 0 721 3906
Size: 721 3185
AvaI C'yCGr_G
Cuts at: 0 4 11 586 636 703 1615 1875 2161
Size: 4 7 575 50 67 912 260 286
Cuts at: 2161 2283 2616 3337 3367 3906
Size: 122 333 721 30 539
Fragments arranged by size:
912 721 575 539 333 286 260 122
67 50 30 7 4
AvrII C'CTAG_G
Cuts at: 0 630 3906
Size: 630 3276
BanI G'GyrC_C
Cuts at: 0 420 445 624 1336 1951 3333 3508 3697
Size: 420 25 179 712 615 1382 175 189
Cuts at: 3697 3906
Size: 209
Fragments arranged by size:
1382 712 615 420 209 189 179 175
25
BanII G_rGCy'C
Cuts at: 0 11 259 313 486 587 739 1228 1486
Size: 11 248 54 173 101 152 489 258
Cuts at: 1486 1777 1868 2290 2662 3229 3304 3344 3376
Size: 291 91 422 372 567 75 40 32
Cuts at: 3376 3906
Size: 530
Fragments arranged by size:
567 530 489 422 372 291 258 248
173 152 101 91 75 54 40 32
11
BbeI G_GCGC'C
Cuts at: 0 1955 3512 3701 3906
Size: 1955 1557 189 205
Fragments arranged by size:
1955 1557 205 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 2108 3906
Size: 905 1203 1798
Fragments arranged by size:
1798 1203 905
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 396 2976 3010 3906
Size: 362 34 2580 34 896
Fragments arranged by size:
2580 896 362 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1369 3906
Size: 1369 2537
BglI GCCn_nnn'nGGC
Cuts at: 0 110 1145 2141 2210 2435 3278 3494 3906
Size: 110 1035 996 69 225 843 216 412
Fragments arranged by size:
1035 996 843 412 225 216 110 69
BglII A'GATC_T
Cuts at: 0 644 1355 3906
Size: 644 711 2551
Fragments arranged by size:
2551 711 644
BlpI GC'TnA_GC
Cuts at: 0 2886 3906
Size: 2886 1020
Bme1580I G_kGCm'C
Cuts at: 0 11 337 425 486 587 629 725 1777
Size: 11 326 88 61 101 42 96 1052
Cuts at: 1777 2310 3338 3344 3390 3529 3906
Size: 533 1028 6 46 139 377
Fragments arranged by size:
1052 1028 533 377 326 139 101 96
88 61 46 42 11 6
BmgBI CAC'GTC
Cuts at: 0 2229 2376 3105 3243 3405 3906
Size: 2229 147 729 138 162 501
Fragments arranged by size:
2229 729 501 162 147 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 413 2869 3906
Size: 413 2456 1037
Fragments arranged by size:
2456 1037 413
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 543 1641 3906
Size: 543 1098 2265
Fragments arranged by size:
2265 1098 543
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3432 3906
Size: 3432 474
Bpu10I CC'TnA_GC
Cuts at: 0 456 469 1229 3223 3906
Size: 456 13 760 1994 683
Fragments arranged by size:
1994 760 683 456 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 3906
Size: 208 3698
BsaAI yAC'GTr
Cuts at: 0 997 1947 3906
Size: 997 950 1959
Fragments arranged by size:
1959 997 950
BsaHI Gr'CG_yC
Cuts at: 0 1952 3509 3698 3734 3906
Size: 1952 1557 189 36 172
Fragments arranged by size:
1952 1557 189 172 36
BsaWI w'CCGG_w
Cuts at: 0 516 1191 1476 1545 1811 2456 3906
Size: 516 675 285 69 266 645 1450
Fragments arranged by size:
1450 675 645 516 285 266 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1105 1135 1581 1611 1751 1781 3007 3037
Size: 1105 30 446 30 140 30 1226 30
Cuts at: 3037 3906
Size: 869
Fragments arranged by size:
1226 1105 869 446 140 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 357 446 1210 1570 2734 3655 3906
Size: 357 89 764 360 1164 921 251
Fragments arranged by size:
1164 921 764 360 357 251 89
BseYI C'CCAG_C
Cuts at: 0 99 605 764 956 1362 1366 1466 1823
Size: 99 506 159 192 406 4 100 357
Cuts at: 1823 2368 2810 2843 2961 3503 3893 3906
Size: 545 442 33 118 542 390 13
Fragments arranged by size:
545 542 506 442 406 390 357 192
159 118 100 99 33 13 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 997 1426 1588 1927 2283 2625 3165
Size: 679 318 429 162 339 356 342 540
Cuts at: 3165 3906
Size: 741
Fragments arranged by size:
741 679 540 429 356 342 339 318
162
BsiEI CG_ry'CG
Cuts at: 0 134 805 2337 3351 3380 3523 3691 3906
Size: 134 671 1532 1014 29 143 168 215
Fragments arranged by size:
1532 1014 671 215 168 143 134 29
BsiHKAI G_wGCw'C
Cuts at: 0 367 725 739 1235 3906
Size: 367 358 14 496 2671
Fragments arranged by size:
2671 496 367 358 14
BsmI GAATG_Cn'
Cuts at: 0 1990 3100 3906
Size: 1990 1110 806
Fragments arranged by size:
1990 1110 806
Bsp1286I G_dGCh'C
Cuts at: 0 11 259 313 337 367 425 486 587
Size: 11 248 54 24 30 58 61 101
Cuts at: 587 629 725 739 1228 1235 1486 1777 1868
Size: 42 96 14 489 7 251 291 91
Cuts at: 1868 2290 2310 2662 3229 3304 3338 3344 3376
Size: 422 20 352 567 75 34 6 32
Cuts at: 3376 3390 3529 3906
Size: 14 139 377
Fragments arranged by size:
567 489 422 377 352 291 251 248
139 101 96 91 75 61 58 54
42 34 32 30 24 20 14 14
11 7 6
BspEI T'CCGG_A
Cuts at: 0 516 1476 3906
Size: 516 960 2430
Fragments arranged by size:
2430 960 516
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 779 3095 3302 3906
Size: 779 2316 207 604
Fragments arranged by size:
2316 779 604 207
BsrBI CCG'CTC
Cuts at: 0 141 3396 3906
Size: 141 3255 510
Fragments arranged by size:
3255 510 141
BsrDI GCAATG_nn'
Cuts at: 0 1082 1178 3549 3906
Size: 1082 96 2371 357
Fragments arranged by size:
2371 1082 357 96
BsrFI r'CCGG_y
Cuts at: 0 129 133 198 436 448 1545 1811 1900
Size: 129 4 65 238 12 1097 266 89
Cuts at: 1900 2069 2839 3853 3906
Size: 169 770 1014 53
Fragments arranged by size:
1097 1014 770 266 238 169 129 89
65 53 12 4
BssHII G'CGCG_C
Cuts at: 0 1307 3862 3906
Size: 1307 2555 44
Fragments arranged by size:
2555 1307 44
BssSI C'ACGA_G
Cuts at: 0 1965 3906
Size: 1965 1941
BstAPI GCAn_nnn'nTGC
Cuts at: 0 774 1052 2255 2585 3906
Size: 774 278 1203 330 1321
Fragments arranged by size:
1321 1203 774 330 278
BstEII G'GTnAC_C
Cuts at: 0 1530 3906
Size: 1530 2376
BstYI r'GATC_y
Cuts at: 0 644 1355 2410 3906
Size: 644 711 1055 1496
Fragments arranged by size:
1496 1055 711 644
Bsu36I CC'TnA_GG
Cuts at: 0 1505 3906
Size: 1505 2401
BtgI C'CryG_G
Cuts at: 0 674 1095 1535 1755 1929 2634 3498 3685
Size: 674 421 440 220 174 705 864 187
Cuts at: 3685 3906
Size: 221
Fragments arranged by size:
864 705 674 440 421 221 220 187
174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2163 2693 3545 3906
Size: 2163 530 852 361
Fragments arranged by size:
2163 852 530 361
BtsI GCAGTG_nn'
Cuts at: 0 920 1050 1293 1794 1920 2463 2853 3906
Size: 920 130 243 501 126 543 390 1053
Fragments arranged by size:
1053 920 543 501 390 243 130 126
EaeI y'GGCC_r
Cuts at: 0 131 285 357 533 802 1146 1752 2334
Size: 131 154 72 176 269 344 606 582
Cuts at: 2334 2795 3279 3348 3377 3452 3672 3688 3906
Size: 461 484 69 29 75 220 16 218
Fragments arranged by size:
606 582 484 461 344 269 220 218
176 154 131 75 72 69 29 16
EagI C'GGCC_G
Cuts at: 0 131 802 2334 3348 3377 3688 3906
Size: 131 671 1532 1014 29 311 218
Fragments arranged by size:
1532 1014 671 311 218 131 29
EarI CTCTTCn'nnn_
Cuts at: 0 1195 1514 3227 3906
Size: 1195 319 1713 679
Fragments arranged by size:
1713 1195 679 319
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1204 3906
Size: 1204 2702
EcoICRI GAG'CTC
Cuts at: 0 737 3906
Size: 737 3169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2638 3432 3906
Size: 2638 794 474
Fragments arranged by size:
2638 794 474
EcoO109I rG'GnC_Cy
Cuts at: 0 8 460 482 483 595 663 988 1159
Size: 8 452 22 1 112 68 325 171
Cuts at: 1159 1774 2121 2216 2613 2639 3258 3341 3545
Size: 615 347 95 397 26 619 83 204
Cuts at: 3545 3881 3906
Size: 336 25
Fragments arranged by size:
619 615 452 397 347 336 325 204
171 112 95 83 68 26 25 22
8 1
EcoRV GAT'ATC
Cuts at: 0 3759 3906
Size: 3759 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 207 3084 3906
Size: 207 2877 822
Fragments arranged by size:
2877 822 207
FseI GG_CCGG'CC
Cuts at: 0 133 3906
Size: 133 3773
FspI TGC'GCA
Cuts at: 0 1074 3906
Size: 1074 2832
HaeII r_GCGC'y
Cuts at: 0 76 166 180 411 502 1013 1323 1688
Size: 76 90 14 231 91 511 310 365
Cuts at: 1688 1955 1965 3216 3512 3701 3813 3900 3906
Size: 267 10 1251 296 189 112 87 6
Fragments arranged by size:
1251 511 365 310 296 267 231 189
112 91 90 87 76 14 10 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1546 1578 1614 1646 2113 2145 3906
Size: 1546 32 36 32 467 32 1761
Fragments arranged by size:
1761 1546 467 36 32 32 32
HincII GTy'rAC
Cuts at: 0 2949 3291 3906
Size: 2949 342 615
Fragments arranged by size:
2949 615 342
KasI G'GCGC_C
Cuts at: 0 1951 3508 3697 3906
Size: 1951 1557 189 209
Fragments arranged by size:
1951 1557 209 189
KpnI G_GTAC'C
Cuts at: 0 449 3906
Size: 449 3457
MfeI C'AATT_G
Cuts at: 0 1914 3906
Size: 1914 1992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 2981 3152 3906
Size: 284 2697 171 754
Fragments arranged by size:
2697 754 284 171
MscI TGG'CCA
Cuts at: 0 535 1148 1754 2797 3906
Size: 535 613 606 1043 1109
Fragments arranged by size:
1109 1043 613 606 535
MslI CAynn'nnrTG
Cuts at: 0 973 1891 2714 2803 3906
Size: 973 918 823 89 1103
Fragments arranged by size:
1103 973 918 823 89
MspA1I CmG'CkG
Cuts at: 0 830 960 1342 1366 1931 2297 3173 3284
Size: 830 130 382 24 565 366 876 111
Cuts at: 3284 3311 3500 3677 3687 3749 3827 3839 3906
Size: 27 189 177 10 62 78 12 67
Fragments arranged by size:
876 830 565 382 366 189 177 130
111 78 67 62 27 24 12 10
NaeI GCC'GGC
Cuts at: 0 131 135 2841 3855 3906
Size: 131 4 2706 1014 51
Fragments arranged by size:
2706 1014 131 51 4
NarI GG'CG_CC
Cuts at: 0 1952 3509 3698 3906
Size: 1952 1557 189 208
Fragments arranged by size:
1952 1557 208 189
NcoI C'CATG_G
Cuts at: 0 1095 2634 3906
Size: 1095 1539 1272
Fragments arranged by size:
1539 1272 1095
NgoMIV G'CCGG_C
Cuts at: 0 129 133 2839 3853 3906
Size: 129 4 2706 1014 53
Fragments arranged by size:
2706 1014 129 53 4
NruI TCG'CGA
Cuts at: 0 372 2225 3906
Size: 372 1853 1681
Fragments arranged by size:
1853 1681 372
NspI r_CATG'y
Cuts at: 0 2991 3438 3906
Size: 2991 447 468
Fragments arranged by size:
2991 468 447
PasI CC'CwG_GG
Cuts at: 0 822 1023 2310 2556 3329 3906
Size: 822 201 1287 246 773 577
Fragments arranged by size:
1287 822 773 577 246 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 1333 1652 3906
Size: 1333 319 2254
Fragments arranged by size:
2254 1333 319
PmlI CAC'GTG
Cuts at: 0 1947 3906
Size: 1947 1959
PpuMI rG'GwC_Cy
Cuts at: 0 2121 2613 3258 3545 3906
Size: 2121 492 645 287 361
Fragments arranged by size:
2121 645 492 361 287
PshAI GACnn'nnGTC
Cuts at: 0 245 3656 3906
Size: 245 3411 250
Fragments arranged by size:
3411 250 245
PspOMI G'GGCC_C
Cuts at: 0 7 482 583 1773 3340 3906
Size: 7 475 101 1190 1567 566
Fragments arranged by size:
1567 1190 566 475 101 7
PstI C_TGCA'G
Cuts at: 0 965 1288 2398 2499 3906
Size: 965 323 1110 101 1407
Fragments arranged by size:
1407 1110 965 323 101
PvuII CAG'CTG
Cuts at: 0 960 1366 3906
Size: 960 406 2540
Fragments arranged by size:
2540 960 406
SacI G_AGCT'C
Cuts at: 0 739 3906
Size: 739 3167
SacII CC_GC'GG
Cuts at: 0 1932 3501 3688 3906
Size: 1932 1569 187 218
Fragments arranged by size:
1932 1569 218 187
SanDI GG'GwC_CC
Cuts at: 0 2121 3545 3906
Size: 2121 1424 361
Fragments arranged by size:
2121 1424 361
SapI GCTCTTCn'nnn_
Cuts at: 0 1195 3906
Size: 1195 2711
SfcI C'TryA_G
Cuts at: 0 961 1284 2394 2495 2538 2880 3906
Size: 961 323 1110 101 43 342 1026
Fragments arranged by size:
1110 1026 961 342 323 101 43
SfoI GGC'GCC
Cuts at: 0 1953 3510 3699 3906
Size: 1953 1557 189 207
Fragments arranged by size:
1953 1557 207 189
SgrAI Cr'CCGG_yG
Cuts at: 0 198 1545 1900 3906
Size: 198 1347 355 2006
Fragments arranged by size:
2006 1347 355 198
SmaI CCC'GGG
Cuts at: 0 6 588 638 1877 2163 2285 3339 3906
Size: 6 582 50 1239 286 122 1054 567
Fragments arranged by size:
1239 1054 582 567 286 122 50 6
SmlI C'TyrA_G
Cuts at: 0 223 3906
Size: 223 3683
SrfI GCCC'GGGC
Cuts at: 0 6 3339 3906
Size: 6 3333 567
Fragments arranged by size:
3333 567 6
StuI AGG'CCT
Cuts at: 0 709 1300 3906
Size: 709 591 2606
Fragments arranged by size:
2606 709 591
StyI C'CwwG_G
Cuts at: 0 630 1095 2634 3906
Size: 630 465 1539 1272
Fragments arranged by size:
1539 1272 630 465
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 826 1063 1262 1473 1595 2563 2713 2944
Size: 826 237 199 211 122 968 150 231
Cuts at: 2944 3321 3906
Size: 377 585
Fragments arranged by size:
968 826 585 377 237 231 211 199
150 122
TatI w'GTAC_w
Cuts at: 0 965 3197 3906
Size: 965 2232 709
Fragments arranged by size:
2232 965 709
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1581 1613 1751 1783 3007 3039 3421 3453
Size: 1581 32 138 32 1224 32 382 32
Cuts at: 3453 3906
Size: 453
Fragments arranged by size:
1581 1224 453 382 138 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1747 3906
Size: 1747 2159
XmaI C'CCGG_G
Cuts at: 0 4 586 636 1875 2161 2283 3337 3906
Size: 4 582 50 1239 286 122 1054 569
Fragments arranged by size:
1239 1054 582 569 286 122 50 4
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AgeI AleI
AlwNI ApaI ApaLI AvaI AvrII BanI BanII BbeI
BbsI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI FseI
FspI HaeII Hin4I HincII KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PflMI PmlI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SanDI SapI SfcI SfoI SgrAI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII AclI AflII AhdI AlfI AloI ApoI AscI
AseI AsiSI BaeI BamHI BbvCI BclI BfrBI BmtI
BsaI BsaBI BsiWI BsmBI BspHI BsrGI BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EcoNI EcoRI
FspAI HindIII HpaI MluI NdeI NheI NotI NsiI
PacI PciI PfoI PmeI PpiI PsiI PspXI PsrI
PvuI RsrII SalI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SspI SwaI Tth111I XbaI XhoI
XmnI ZraI