Restriction map for
pF1KSDF0128
(Linear) (Six-base) MAPSORT of: FLJ00128.seq Check: 1257 from: 1 to: 4557
FLJ00128.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 179 312 2361 4557
Size: 179 133 2049 2196
Fragments arranged by size:
2196 2049 179 133
AatII G_ACGT'C
Cuts at: 0 4370 4557
Size: 4370 187
AccI GT'mk_AC
Cuts at: 0 3143 4557
Size: 3143 1414
Acc65I G'GTAC_C
Cuts at: 0 156 667 810 1108 4557
Size: 156 511 143 298 3449
Fragments arranged by size:
3449 511 298 156 143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 797 1115 1397 1816 3418 3817 3938 4557
Size: 797 318 282 419 1602 399 121 619
Fragments arranged by size:
1602 797 619 419 399 318 282 121
AfeI AGC'GCT
Cuts at: 0 2484 2626 4557
Size: 2484 142 1931
Fragments arranged by size:
2484 1931 142
AflIII A'CryG_T
Cuts at: 0 3168 4557
Size: 3168 1389
AhdI GACnn_n'nnGTC
Cuts at: 0 2439 4557
Size: 2439 2118
AleI CACnn'nnGTG
Cuts at: 0 363 441 3136 4557
Size: 363 78 2695 1421
Fragments arranged by size:
2695 1421 363 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 191 3772 4557
Size: 191 3581 785
Fragments arranged by size:
3581 785 191
AlwNI CAG_nnn'CTG
Cuts at: 0 491 525 775 964 1018 1327 1755 1866
Size: 491 34 250 189 54 309 428 111
Cuts at: 1866 2015 2355 2580 2683 3264 3621 4110 4557
Size: 149 340 225 103 581 357 489 447
Fragments arranged by size:
581 491 489 447 428 357 340 309
250 225 189 149 111 103 54 34
ApaI G_GGCC'C
Cuts at: 0 2395 2613 2733 2772 2934 2945 3061 3705
Size: 2395 218 120 39 162 11 116 644
Cuts at: 3705 3916 4557
Size: 211 641
Fragments arranged by size:
2395 644 641 218 211 162 120 116
39 11
ApaLI G'TGCA_C
Cuts at: 0 559 4034 4557
Size: 559 3475 523
Fragments arranged by size:
3475 559 523
ApoI r'AATT_y
Cuts at: 0 4376 4557
Size: 4376 181
AvaI C'yCGr_G
Cuts at: 0 890 1691 2388 3386 3602 4253 4526 4557
Size: 890 801 697 998 216 651 273 31
Fragments arranged by size:
998 890 801 697 651 273 216 31
BamHI G'GATC_C
Cuts at: 0 2852 4557
Size: 2852 1705
BanI G'GyrC_C
Cuts at: 0 156 327 667 810 1108 1788 2749 2914
Size: 156 171 340 143 298 680 961 165
Cuts at: 2914 3217 3361 4557
Size: 303 144 1196
Fragments arranged by size:
1196 961 680 340 303 298 171 165
156 144 143
BanII G_rGCy'C
Cuts at: 0 542 766 1007 1170 1204 1343 1644 1682
Size: 542 224 241 163 34 139 301 38
Cuts at: 1682 2077 2395 2613 2733 2741 2772 2884 2934
Size: 395 318 218 120 8 31 112 50
Cuts at: 2934 2945 3061 3577 3609 3662 3686 3705 3916
Size: 11 116 516 32 53 24 19 211
Cuts at: 3916 4142 4533 4557
Size: 226 391 24
Fragments arranged by size:
542 516 395 391 318 301 241 226
224 218 211 163 139 120 116 112
53 50 38 34 32 31 24 24
19 11 8
BbvCI CC'TCA_GC
Cuts at: 0 333 965 1367 1922 2486 2965 4557
Size: 333 632 402 555 564 479 1592
Fragments arranged by size:
1592 632 564 555 479 402 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3839 3873 4557
Size: 3839 34 684
Fragments arranged by size:
3839 684 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 62 4557
Size: 62 4495
BclI T'GATC_A
Cuts at: 0 1563 4557
Size: 1563 2994
BglI GCCn_nnn'nGGC
Cuts at: 0 917 1466 2888 3709 3769 4264 4557
Size: 917 549 1422 821 60 495 293
Fragments arranged by size:
1422 917 821 549 495 293 60
BglII A'GATC_T
Cuts at: 0 3792 4557
Size: 3792 765
BlpI GC'TnA_GC
Cuts at: 0 536 4557
Size: 536 4021
Bme1580I G_kGCm'C
Cuts at: 0 204 332 410 563 692 1194 1669 2395
Size: 204 128 78 153 129 502 475 726
Cuts at: 2395 2613 2733 2772 2934 2945 3061 3255 3364
Size: 218 120 39 162 11 116 194 109
Cuts at: 3364 3387 3705 3916 4038 4227 4557
Size: 23 318 211 122 189 330
Fragments arranged by size:
726 502 475 330 318 218 211 204
194 189 162 153 129 128 122 120
116 109 78 39 23 11
BmgBI CAC'GTC
Cuts at: 0 3152 4557
Size: 3152 1405
BmrI ACTGGGnnnn_n'
Cuts at: 0 1765 2003 2025 2735 2928 4557
Size: 1765 238 22 710 193 1629
Fragments arranged by size:
1765 1629 710 238 193 22
BmtI G_CTAG'C
Cuts at: 0 277 4557
Size: 277 4280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4490 4541 4557
Size: 4490 51 16
Fragments arranged by size:
4490 51 16
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 780 851 1441 1519 1787 2122 2269
Size: 611 169 71 590 78 268 335 147
Cuts at: 2269 2350 2551 2602 3056 3139 3556 3673 4430
Size: 81 201 51 454 83 417 117 757
Cuts at: 4430 4557
Size: 127
Fragments arranged by size:
757 611 590 454 417 335 268 201
169 147 127 117 83 81 78 71
51
Bpu10I CC'TnA_GC
Cuts at: 0 8 333 965 1311 1367 1735 1922 2486
Size: 8 325 632 346 56 368 187 564
Cuts at: 2486 2965 3062 3338 3680 4557
Size: 479 97 276 342 877
Fragments arranged by size:
877 632 564 479 368 346 342 325
276 187 97 56 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1842 3330 3549 3892 4066 4557
Size: 346 1496 1488 219 343 174 491
Fragments arranged by size:
1496 1488 491 346 343 219 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 3746 3818 4557
Size: 3746 72 739
Fragments arranged by size:
3746 739 72
BsaAI yAC'GTr
Cuts at: 0 2328 3171 3300 3516 4557
Size: 2328 843 129 216 1041
Fragments arranged by size:
2328 1041 843 216 129
BsaHI Gr'CG_yC
Cuts at: 0 3346 4367 4557
Size: 3346 1021 190
Fragments arranged by size:
3346 1021 190
BsaWI w'CCGG_w
Cuts at: 0 803 848 934 1033 3410 4557
Size: 803 45 86 99 2377 1147
Fragments arranged by size:
2377 1147 803 99 86 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1960 1990 3710 3740 4557
Size: 1960 30 1720 30 817
Fragments arranged by size:
1960 1720 817 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 670 1082 1085 1088 1091 1094 1193 1690
Size: 670 412 3 3 3 3 99 497
Cuts at: 1690 1981 2110 2143 2416 2548 2862 2887 3670
Size: 291 129 33 273 132 314 25 783
Cuts at: 3670 3880 4360 4363 4557
Size: 210 480 3 194
Fragments arranged by size:
783 670 497 480 412 314 291 273
210 194 132 129 99 33 25 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 637 663 1008 2070 2236 2262 2683 2793
Size: 637 26 345 1062 166 26 421 110
Cuts at: 2793 2959 2983 3177 3254 3673 4103 4441 4557
Size: 166 24 194 77 419 430 338 116
Fragments arranged by size:
1062 637 430 421 419 345 338 194
166 166 116 110 77 26 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 289 412 1528 2308 2350 2696 3536
Size: 49 240 123 1116 780 42 346 840
Cuts at: 3536 4168 4557
Size: 632 389
Fragments arranged by size:
1116 840 780 632 389 346 240 123
49 42
BsiHKAI G_wGCw'C
Cuts at: 0 37 542 563 909 1268 1343 1644 3157
Size: 37 505 21 346 359 75 301 1513
Cuts at: 3157 3662 3686 4038 4142 4557
Size: 505 24 352 104 415
Fragments arranged by size:
1513 505 505 415 359 352 346 301
104 75 37 24 21
BsmI GAATG_Cn'
Cuts at: 0 1285 2147 2544 4557
Size: 1285 862 397 2013
Fragments arranged by size:
2013 1285 862 397
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1563 4557
Size: 1563 2994
Bsp1286I G_dGCh'C
Cuts at: 0 37 204 332 410 542 563 692 766
Size: 37 167 128 78 132 21 129 74
Cuts at: 766 909 1007 1170 1194 1204 1268 1343 1644
Size: 143 98 163 24 10 64 75 301
Cuts at: 1644 1669 1682 2077 2395 2613 2733 2741 2772
Size: 25 13 395 318 218 120 8 31
Cuts at: 2772 2884 2934 2945 3061 3157 3255 3364 3387
Size: 112 50 11 116 96 98 109 23
Cuts at: 3387 3577 3609 3662 3686 3705 3916 4038 4142
Size: 190 32 53 24 19 211 122 104
Cuts at: 4142 4227 4533 4557
Size: 85 306 24
Fragments arranged by size:
395 318 306 301 218 211 190 167
163 143 132 129 128 122 120 116
112 109 104 98 98 96 85 78
75 74 64 53 50 37 32 31
25 24 24 24 23 21 19 13
11 10 8
BspEI T'CCGG_A
Cuts at: 0 803 1033 4557
Size: 803 230 3524
Fragments arranged by size:
3524 803 230
BspHI T'CATG_A
Cuts at: 0 1881 4557
Size: 1881 2676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 312 499 1147 2330 2361 2577 3605
Size: 179 133 187 648 1183 31 216 1028
Cuts at: 3605 3764 4557
Size: 159 793
Fragments arranged by size:
1183 1028 793 648 216 187 179 159
133 31
BsrBI CCG'CTC
Cuts at: 0 243 4212 4557
Size: 243 3969 345
Fragments arranged by size:
3969 345 243
BsrDI GCAATG_nn'
Cuts at: 0 3015 4557
Size: 3015 1542
BsrFI r'CCGG_y
Cuts at: 0 467 2746 4288 4557
Size: 467 2279 1542 269
Fragments arranged by size:
2279 1542 467 269
BsrGI T'GTAC_A
Cuts at: 0 3717 4557
Size: 3717 840
BssSI C'ACGA_G
Cuts at: 0 4037 4557
Size: 4037 520
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 327 2163 2355 2376 2419 2914 4557
Size: 297 30 1836 192 21 43 495 1643
Fragments arranged by size:
1836 1643 495 297 192 43 30 21
BstEII G'GTnAC_C
Cuts at: 0 3123 4557
Size: 3123 1434
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 211 4557
Size: 84 127 4346
Fragments arranged by size:
4346 127 84
BstYI r'GATC_y
Cuts at: 0 1036 1845 2172 2852 3792 4359 4496 4557
Size: 1036 809 327 680 940 567 137 61
Fragments arranged by size:
1036 940 809 680 567 327 137 61
Bsu36I CC'TnA_GG
Cuts at: 0 653 713 1048 1673 3664 4557
Size: 653 60 335 625 1991 893
Fragments arranged by size:
1991 893 653 625 335 60
BtgI C'CryG_G
Cuts at: 0 2884 2998 3160 3483 3578 3744 3764 4163
Size: 2884 114 162 323 95 166 20 399
Cuts at: 4163 4262 4557
Size: 99 295
Fragments arranged by size:
2884 399 323 295 166 162 114 99
95 20
BtsI GCAGTG_nn'
Cuts at: 0 598 1729 1795 1981 2141 2152 2188 2356
Size: 598 1131 66 186 160 11 36 168
Cuts at: 2356 2386 2928 4557
Size: 30 542 1629
Fragments arranged by size:
1629 1131 598 542 186 168 160 66
36 30 11
DraIII CAC_nnn'GTG
Cuts at: 0 1261 4557
Size: 1261 3296
EaeI y'GGCC_r
Cuts at: 0 238 469 1955 3001 3302 3761 3846 4265
Size: 238 231 1486 1046 301 459 85 419
Cuts at: 4265 4484 4557
Size: 219 73
Fragments arranged by size:
1486 1046 459 419 301 238 231 219
85 73
EarI CTCTTCn'nnn_
Cuts at: 0 226 1391 1532 1718 2120 3047 3883 4233
Size: 226 1165 141 186 402 927 836 350
Cuts at: 4233 4410 4557
Size: 177 147
Fragments arranged by size:
1165 927 836 402 350 226 186 177
147 141
EcoICRI GAG'CTC
Cuts at: 0 540 1341 1642 3660 3684 4140 4557
Size: 540 801 301 2018 24 456 417
Fragments arranged by size:
2018 801 540 456 417 301 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 780 797 851 1115 1397 1441 1519
Size: 611 169 17 54 264 282 44 78
Cuts at: 1519 1787 1816 2122 2269 2350 2551 2602 3056
Size: 268 29 306 147 81 201 51 454
Cuts at: 3056 3139 3418 3556 3673 3817 3938 4430 4557
Size: 83 279 138 117 144 121 492 127
Fragments arranged by size:
611 492 454 306 282 279 268 264
201 169 147 144 138 127 121 117
83 81 78 54 51 44 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 704 1291 1783 2506 2869 4557
Size: 704 587 492 723 363 1688
Fragments arranged by size:
1688 723 704 587 492 363
EcoO109I rG'GnC_Cy
Cuts at: 0 570 709 879 886 1468 1542 1998 2520
Size: 570 139 170 7 582 74 456 522
Cuts at: 2520 2610 2730 2769 2930 2941 2942 3057 3058
Size: 90 120 39 161 11 1 115 1
Cuts at: 3058 3089 3467 3702 3912 3913 4557
Size: 31 378 235 210 1 644
Fragments arranged by size:
644 582 570 522 456 378 235 210
170 161 139 120 115 90 74 39
31 11 7 1 1 1
EcoRV GAT'ATC
Cuts at: 0 2565 4557
Size: 2565 1992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1255 1561 3969 4557
Size: 1255 306 2408 588
Fragments arranged by size:
2408 1255 588 306
HaeII r_GCGC'y
Cuts at: 0 664 830 2486 2502 2628 4557
Size: 664 166 1656 16 126 1929
Fragments arranged by size:
1929 1656 664 166 126 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 344 376 1592 1624 1915 1947
Size: 102 32 210 32 1216 32 291 32
Cuts at: 1947 2425 2457 4557
Size: 478 32 2100
Fragments arranged by size:
2100 1216 478 291 210 102 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1270 2415 4557
Size: 1270 1145 2142
Fragments arranged by size:
2142 1270 1145
KpnI G_GTAC'C
Cuts at: 0 160 671 814 1112 4557
Size: 160 511 143 298 3445
Fragments arranged by size:
3445 511 298 160 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 561 1510 1918 2342 4557
Size: 561 949 408 424 2215
Fragments arranged by size:
2215 949 561 424 408
MscI TGG'CCA
Cuts at: 0 1957 3304 4486 4557
Size: 1957 1347 1182 71
Fragments arranged by size:
1957 1347 1182 71
MslI CAynn'nnrTG
Cuts at: 0 363 441 1571 2215 2646 3136 4557
Size: 363 78 1130 644 431 490 1421
Fragments arranged by size:
1421 1130 644 490 431 363 78
MspA1I CmG'CkG
Cuts at: 0 337 522 1497 1863 2346 2409 2577 2819
Size: 337 185 975 366 483 63 168 242
Cuts at: 2819 2831 3261 3630 3723 3998 4107 4557
Size: 12 430 369 93 275 109 450
Fragments arranged by size:
975 483 450 430 369 366 337 275
242 185 168 109 93 63 12
NaeI GCC'GGC
Cuts at: 0 2748 4290 4557
Size: 2748 1542 267
Fragments arranged by size:
2748 1542 267
NcoI C'CATG_G
Cuts at: 0 3578 4163 4557
Size: 3578 585 394
Fragments arranged by size:
3578 585 394
NgoMIV G'CCGG_C
Cuts at: 0 2746 4288 4557
Size: 2746 1542 269
Fragments arranged by size:
2746 1542 269
NheI G'CTAG_C
Cuts at: 0 273 4557
Size: 273 4284
NspI r_CATG'y
Cuts at: 0 4288 4557
Size: 4288 269
PasI CC'CwG_GG
Cuts at: 0 376 530 1204 2395 2992 3206 3331 4462
Size: 376 154 674 1191 597 214 125 1131
Cuts at: 4462 4557
Size: 95
Fragments arranged by size:
1191 1131 674 597 376 214 154 125
95
PflMI CCAn_nnn'nTGG
Cuts at: 0 105 2015 2037 3331 4557
Size: 105 1910 22 1294 1226
Fragments arranged by size:
1910 1294 1226 105 22
PfoI T'CCnGG_A
Cuts at: 0 2492 2756 3650 3728 4187 4557
Size: 2492 264 894 78 459 370
Fragments arranged by size:
2492 894 459 370 264 78
PmlI CAC'GTG
Cuts at: 0 2328 3171 4557
Size: 2328 843 1386
Fragments arranged by size:
2328 1386 843
PpuMI rG'GwC_Cy
Cuts at: 0 709 1998 4557
Size: 709 1289 2559
Fragments arranged by size:
2559 1289 709
PshAI GACnn'nnGTC
Cuts at: 0 1781 4557
Size: 1781 2776
PspOMI G'GGCC_C
Cuts at: 0 2391 2609 2729 2768 2930 2941 3057 3701
Size: 2391 218 120 39 162 11 116 644
Cuts at: 3701 3912 4557
Size: 211 645
Fragments arranged by size:
2391 645 644 218 211 162 120 116
39 11
PspXI vC'TCGA_Gb
Cuts at: 0 4526 4557
Size: 4526 31
PstI C_TGCA'G
Cuts at: 0 323 1158 1976 2159 2195 2453 3614 4058
Size: 323 835 818 183 36 258 1161 444
Cuts at: 4058 4557
Size: 499
Fragments arranged by size:
1161 835 818 499 444 323 258 183
36
PvuII CAG'CTG
Cuts at: 0 337 2409 3630 3723 4107 4557
Size: 337 2072 1221 93 384 450
Fragments arranged by size:
2072 1221 450 384 337 93
SacI G_AGCT'C
Cuts at: 0 542 1343 1644 3662 3686 4142 4557
Size: 542 801 301 2018 24 456 415
Fragments arranged by size:
2018 801 542 456 415 301 24
SanDI GG'GwC_CC
Cuts at: 0 1998 4557
Size: 1998 2559
SapI GCTCTTCn'nnn_
Cuts at: 0 4233 4557
Size: 4233 324
SbfI CC_TGCA'GG
Cuts at: 0 2453 4557
Size: 2453 2104
SexAI A'CCwGG_T
Cuts at: 0 1104 1286 4557
Size: 1104 182 3271
Fragments arranged by size:
3271 1104 182
SfcI C'TryA_G
Cuts at: 0 319 1154 1391 1747 1972 2155 2191 2449
Size: 319 835 237 356 225 183 36 258
Cuts at: 2449 2620 3610 4054 4557
Size: 171 990 444 503
Fragments arranged by size:
990 835 503 444 356 319 258 237
225 183 171 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1466 4264 4557
Size: 1466 2798 293
Fragments arranged by size:
2798 1466 293
SmaI CCC'GGG
Cuts at: 0 3388 4255 4557
Size: 3388 867 302
Fragments arranged by size:
3388 867 302
SmlI C'TyrA_G
Cuts at: 0 361 1821 3309 3564 3907 4081 4526 4557
Size: 361 1460 1488 255 343 174 445 31
Fragments arranged by size:
1488 1460 445 361 343 255 174 31
SphI G_CATG'C
Cuts at: 0 4288 4557
Size: 4288 269
StuI AGG'CCT
Cuts at: 0 791 1433 1740 3116 3952 4354 4557
Size: 791 642 307 1376 836 402 203
Fragments arranged by size:
1376 836 791 642 402 307 203
StyI C'CwwG_G
Cuts at: 0 179 1239 2061 2256 3578 3705 4163 4203
Size: 179 1060 822 195 1322 127 458 40
Cuts at: 4203 4557
Size: 354
Fragments arranged by size:
1322 1060 822 458 354 195 179 127
40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2640 3463 4557
Size: 2640 823 1094
Fragments arranged by size:
2640 1094 823
TatI w'GTAC_w
Cuts at: 0 595 3717 4557
Size: 595 3122 840
Fragments arranged by size:
3122 840 595
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1960 1992 4206 4238 4557
Size: 1960 32 2214 32 319
Fragments arranged by size:
2214 1960 319 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4517 4557
Size: 4517 40
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1237 2097 2244 3135 3203 4557
Size: 1237 860 147 891 68 1354
Fragments arranged by size:
1354 1237 891 860 147 68
XhoI C'TCGA_G
Cuts at: 0 4526 4557
Size: 4526 31
XmaI C'CCGG_G
Cuts at: 0 3386 4253 4557
Size: 3386 867 304
Fragments arranged by size:
3386 867 304
XmnI GAAnn'nnTTC
Cuts at: 0 3395 4557
Size: 3395 1162
ZraI GAC'GTC
Cuts at: 0 4368 4557
Size: 4368 189
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI
BanI BanII BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I HindIII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PasI PflMI PfoI PmlI PpuMI PshAI
PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SfiI SmaI SmlI SphI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BfrBI BsaBI BsiEI BsiWI BssHII
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoRI FseI FspI FspAI HincII HpaI KasI
MfeI MluI NarI NdeI NotI NruI NsiI PacI
PciI PmeI PpiI PsiI PsrI PvuI RsrII SacII
SalI ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI XbaI