Restriction map for
pF1KF0141
(Linear) (Six-base) MAPSORT of: FLJ00141.seq Check: 9256 from: 1 to: 3957
FLJ00141.orf
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3149 3809 3957
Size: 3149 660 148
Fragments arranged by size:
3149 660 148
Acc65I G'GTAC_C
Cuts at: 0 852 3957
Size: 852 3105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1584 3043 3583 3772 3957
Size: 428 1156 1459 540 189 185
Fragments arranged by size:
1459 1156 540 428 189 185
AfeI AGC'GCT
Cuts at: 0 2041 2880 3685 3957
Size: 2041 839 805 272
Fragments arranged by size:
2041 839 805 272
AflII C'TTAA_G
Cuts at: 0 1377 3957
Size: 1377 2580
AflIII A'CryG_T
Cuts at: 0 3266 3957
Size: 3266 691
AhdI GACnn_n'nnGTC
Cuts at: 0 3267 3957
Size: 3267 690
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2123 3957
Size: 2123 1834
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2410 2442 3957
Size: 2410 32 1515
Fragments arranged by size:
2410 1515 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1012 1105 1165 1839 2169 2220 2487 2637
Size: 1012 93 60 674 330 51 267 150
Cuts at: 2637 3396 3468 3931 3957
Size: 759 72 463 26
Fragments arranged by size:
1012 759 674 463 330 267 150 93
72 60 51 26
ApaI G_GGCC'C
Cuts at: 0 232 461 3097 3957
Size: 232 229 2636 860
Fragments arranged by size:
2636 860 232 229
AvaI C'yCGr_G
Cuts at: 0 2234 2609 3099 3957
Size: 2234 375 490 858
Fragments arranged by size:
2234 858 490 375
BanI G'GyrC_C
Cuts at: 0 274 718 852 1581 1704 3957
Size: 274 444 134 729 123 2253
Fragments arranged by size:
2253 729 444 274 134 123
BanII G_rGCy'C
Cuts at: 0 232 323 453 461 574 736 1048 1404
Size: 232 91 130 8 113 162 312 356
Cuts at: 1404 1555 1618 1636 2163 2580 3097 3208 3823
Size: 151 63 18 527 417 517 111 615
Cuts at: 3823 3957
Size: 134
Fragments arranged by size:
615 527 517 417 356 312 232 162
151 134 130 113 111 91 63 18
8
BbeI G_GCGC'C
Cuts at: 0 722 3957
Size: 722 3235
BbsI GAAGACnn'nnnn_
Cuts at: 0 2719 3571 3957
Size: 2719 852 386
Fragments arranged by size:
2719 852 386
BbvCI CC'TCA_GC
Cuts at: 0 3041 3932 3957
Size: 3041 891 25
Fragments arranged by size:
3041 891 25
BglI GCCn_nnn'nGGC
Cuts at: 0 342 1346 1495 2239 3101 3150 3957
Size: 342 1004 149 744 862 49 807
Fragments arranged by size:
1004 862 807 744 342 149 49
BlpI GC'TnA_GC
Cuts at: 0 937 1612 2504 2839 3017 3619 3957
Size: 937 675 892 335 178 602 338
Fragments arranged by size:
937 892 675 602 338 335 178
Bme1580I G_kGCm'C
Cuts at: 0 232 277 461 845 1533 2177 2259 2292
Size: 232 45 184 384 688 644 82 33
Cuts at: 2292 3097 3456 3957
Size: 805 359 501
Fragments arranged by size:
805 688 644 501 384 359 232 184
82 45 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 809 1576 1652 3957
Size: 809 767 76 2305
Fragments arranged by size:
2305 809 767 76
BmtI G_CTAG'C
Cuts at: 0 2143 3957
Size: 2143 1814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 768 1530 3957
Size: 768 762 2427
Fragments arranged by size:
2427 768 762
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 227 647 720 740 852 1142 1177
Size: 196 31 420 73 20 112 290 35
Cuts at: 1177 1211 1671 1789 1861 2110 2413 3220 3418
Size: 34 460 118 72 249 303 807 198
Cuts at: 3418 3957
Size: 539
Fragments arranged by size:
807 539 460 420 303 290 249 198
196 118 112 73 72 35 34 31
20
Bpu10I CC'TnA_GC
Cuts at: 0 2162 2579 3041 3932 3957
Size: 2162 417 462 891 25
Fragments arranged by size:
2162 891 462 417 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2044 2173 3691 3957
Size: 2044 129 1518 266
Fragments arranged by size:
2044 1518 266 129
BsaI GGTCTCn'nnnn_
Cuts at: 0 1837 2941 3214 3957
Size: 1837 1104 273 743
Fragments arranged by size:
1837 1104 743 273
BsaAI yAC'GTr
Cuts at: 0 2178 3612 3957
Size: 2178 1434 345
Fragments arranged by size:
2178 1434 345
BsaHI Gr'CG_yC
Cuts at: 0 719 3957
Size: 719 3238
BsaWI w'CCGG_w
Cuts at: 0 2492 3542 3957
Size: 2492 1050 415
Fragments arranged by size:
2492 1050 415
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 634 2011 2080 2473 2476 2796 2799 2814
Size: 634 1377 69 393 3 320 3 15
Cuts at: 2814 2817 2820 3181 3319 3415 3418 3957
Size: 3 3 361 138 96 3 539
Fragments arranged by size:
1377 634 539 393 361 320 138 96
69 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 967 1053 1098 1207 1481 1524 1617 1897
Size: 967 86 45 109 274 43 93 280
Cuts at: 1897 1909 2090 2169 2563 2834 3087 3828 3957
Size: 12 181 79 394 271 253 741 129
Fragments arranged by size:
967 741 394 280 274 271 253 181
129 109 93 86 79 45 43 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 291 2091 2608 3592 3830 3957
Size: 76 215 1800 517 984 238 127
Fragments arranged by size:
1800 984 517 238 215 127 76
BsiHKAI G_wGCw'C
Cuts at: 0 251 574 918 1113 1404 2089 2102 3179
Size: 251 323 344 195 291 685 13 1077
Cuts at: 3179 3208 3620 3957
Size: 29 412 337
Fragments arranged by size:
1077 685 412 344 337 323 291 251
195 29 13
BsmI GAATG_Cn'
Cuts at: 0 1130 2625 3957
Size: 1130 1495 1332
Fragments arranged by size:
1495 1332 1130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3736 3957
Size: 3736 221
Bsp1286I G_dGCh'C
Cuts at: 0 232 251 277 323 453 461 574 736
Size: 232 19 26 46 130 8 113 162
Cuts at: 736 845 918 1048 1113 1404 1533 1555 1618
Size: 109 73 130 65 291 129 22 63
Cuts at: 1618 1636 2089 2102 2163 2177 2259 2292 2580
Size: 18 453 13 61 14 82 33 288
Cuts at: 2580 3097 3179 3208 3456 3620 3823 3957
Size: 517 82 29 248 164 203 134
Fragments arranged by size:
517 453 291 288 248 232 203 164
162 134 130 130 129 113 109 82
82 73 65 63 61 46 33 29
26 22 19 18 14 13 8
BspEI T'CCGG_A
Cuts at: 0 2492 3542 3957
Size: 2492 1050 415
Fragments arranged by size:
2492 1050 415
BspHI T'CATG_A
Cuts at: 0 3950 3957
Size: 3950 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 596 701 864 3149 3414 3542 3610
Size: 275 321 105 163 2285 265 128 68
Cuts at: 3610 3809 3957
Size: 199 148
Fragments arranged by size:
2285 321 275 265 199 163 148 128
105 68
BsrBI CCG'CTC
Cuts at: 0 1239 1722 1797 2157 2253 2286 2442 2744
Size: 1239 483 75 360 96 33 156 302
Cuts at: 2744 3369 3408 3957
Size: 625 39 549
Fragments arranged by size:
1239 625 549 483 360 302 156 96
75 39 33
BsrDI GCAATG_nn'
Cuts at: 0 306 3957
Size: 306 3651
BsrFI r'CCGG_y
Cuts at: 0 1340 1778 2476 3347 3701 3860 3957
Size: 1340 438 698 871 354 159 97
Fragments arranged by size:
1340 871 698 438 354 159 97
BssSI C'ACGA_G
Cuts at: 0 1961 3957
Size: 1961 1996
BstAPI GCAn_nnn'nTGC
Cuts at: 0 305 365 2220 3615 3632 3957
Size: 305 60 1855 1395 17 325
Fragments arranged by size:
1855 1395 325 305 60 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 729 3957
Size: 729 3228
BstYI r'GATC_y
Cuts at: 0 1434 2570 3957
Size: 1434 1136 1387
Fragments arranged by size:
1434 1387 1136
Bsu36I CC'TnA_GG
Cuts at: 0 1892 3957
Size: 1892 2065
BtgI C'CryG_G
Cuts at: 0 50 1491 3588 3636 3957
Size: 50 1441 2097 48 321
Fragments arranged by size:
2097 1441 321 50 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2505 3957
Size: 2505 1452
BtsI GCAGTG_nn'
Cuts at: 0 144 249 795 1239 2257 2290 2321 2932
Size: 144 105 546 444 1018 33 31 611
Cuts at: 2932 3813 3957
Size: 881 144
Fragments arranged by size:
1018 881 611 546 444 144 144 105
33 31
DraIII CAC_nnn'GTG
Cuts at: 0 126 817 1570 3615 3957
Size: 126 691 753 2045 342
Fragments arranged by size:
2045 753 691 342 126
EaeI y'GGCC_r
Cuts at: 0 1076 2474 2478 2543 3957
Size: 1076 1398 4 65 1414
Fragments arranged by size:
1414 1398 1076 65 4
EarI CTCTTCn'nnn_
Cuts at: 0 780 2147 2823 2990 3957
Size: 780 1367 676 167 967
Fragments arranged by size:
1367 967 780 676 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 2001 3426 3957
Size: 1036 965 1425 531
Fragments arranged by size:
1425 1036 965 531
EcoICRI GAG'CTC
Cuts at: 0 572 1402 3206 3957
Size: 572 830 1804 751
Fragments arranged by size:
1804 830 751 572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 227 428 647 720 740 852 1142
Size: 196 31 201 219 73 20 112 290
Cuts at: 1142 1177 1211 1584 1671 1789 1861 2110 2413
Size: 35 34 373 87 118 72 249 303
Cuts at: 2413 3043 3220 3418 3583 3772 3957
Size: 630 177 198 165 189 185
Fragments arranged by size:
630 373 303 290 249 219 201 198
196 189 185 177 165 118 112 87
73 72 35 34 31 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2731 3400 3957
Size: 2731 669 557
Fragments arranged by size:
2731 669 557
EcoO109I rG'GnC_Cy
Cuts at: 0 28 64 228 458 535 585 832 1347
Size: 28 36 164 230 77 50 247 515
Cuts at: 1347 1888 1955 1976 2388 3094 3393 3957
Size: 541 67 21 412 706 299 564
Fragments arranged by size:
706 564 541 515 412 299 247 230
164 77 67 50 36 28 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1614 3570 3957
Size: 1614 1956 387
Fragments arranged by size:
1956 1614 387
FseI GG_CCGG'CC
Cuts at: 0 2480 3957
Size: 2480 1477
FspI TGC'GCA
Cuts at: 0 3805 3957
Size: 3805 152
HaeII r_GCGC'y
Cuts at: 0 690 722 1482 1791 1839 2043 2541 2882
Size: 690 32 760 309 48 204 498 341
Cuts at: 2882 2958 3687 3957
Size: 76 729 270
Fragments arranged by size:
760 729 690 498 341 309 270 204
76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2562 2594 3253 3285 3957
Size: 2562 32 659 32 672
Fragments arranged by size:
2562 672 659 32 32
HincII GTy'rAC
Cuts at: 0 1120 3957
Size: 1120 2837
KasI G'GCGC_C
Cuts at: 0 718 3957
Size: 718 3239
KpnI G_GTAC'C
Cuts at: 0 856 3957
Size: 856 3101
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2417 3957
Size: 2417 1540
MscI TGG'CCA
Cuts at: 0 1078 2545 3957
Size: 1078 1467 1412
Fragments arranged by size:
1467 1412 1078
MslI CAynn'nnrTG
Cuts at: 0 727 2105 3182 3957
Size: 727 1378 1077 775
Fragments arranged by size:
1378 1077 775 727
MspA1I CmG'CkG
Cuts at: 0 174 849 1065 1413 1691 2220 2325 2634
Size: 174 675 216 348 278 529 105 309
Cuts at: 2634 2792 3003 3040 3091 3468 3957
Size: 158 211 37 51 377 489
Fragments arranged by size:
675 529 489 377 348 309 278 216
211 174 158 105 51 37
NaeI GCC'GGC
Cuts at: 0 1342 2478 3957
Size: 1342 1136 1479
Fragments arranged by size:
1479 1342 1136
NarI GG'CG_CC
Cuts at: 0 719 3957
Size: 719 3238
NcoI C'CATG_G
Cuts at: 0 50 1491 3588 3957
Size: 50 1441 2097 369
Fragments arranged by size:
2097 1441 369 50
NgoMIV G'CCGG_C
Cuts at: 0 1340 2476 3957
Size: 1340 1136 1481
Fragments arranged by size:
1481 1340 1136
NheI G'CTAG_C
Cuts at: 0 2139 3957
Size: 2139 1818
NspI r_CATG'y
Cuts at: 0 363 3270 3957
Size: 363 2907 687
Fragments arranged by size:
2907 687 363
PasI CC'CwG_GG
Cuts at: 0 454 2853 3957
Size: 454 2399 1104
Fragments arranged by size:
2399 1104 454
PciI A'CATG_T
Cuts at: 0 3266 3957
Size: 3266 691
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 1105 1272 1839 2436 3957
Size: 51 1054 167 567 597 1521
Fragments arranged by size:
1521 1054 597 567 167 51
PfoI T'CCnGG_A
Cuts at: 0 1193 1766 1802 2549 3957
Size: 1193 573 36 747 1408
Fragments arranged by size:
1408 1193 747 573 36
PmlI CAC'GTG
Cuts at: 0 2178 3612 3957
Size: 2178 1434 345
Fragments arranged by size:
2178 1434 345
PpuMI rG'GwC_Cy
Cuts at: 0 64 535 585 832 1888 3957
Size: 64 471 50 247 1056 2069
Fragments arranged by size:
2069 1056 471 247 64 50
PspOMI G'GGCC_C
Cuts at: 0 228 457 3093 3957
Size: 228 229 2636 864
Fragments arranged by size:
2636 864 229 228
PstI C_TGCA'G
Cuts at: 0 207 483 1008 1316 2225 2267 2300 2330
Size: 207 276 525 308 909 42 33 30
Cuts at: 2330 2939 2972 3443 3891 3957
Size: 609 33 471 448 66
Fragments arranged by size:
909 609 525 471 448 308 276 207
66 42 33 33 30
PvuII CAG'CTG
Cuts at: 0 1065 2220 2325 2634 3040 3468 3957
Size: 1065 1155 105 309 406 428 489
Fragments arranged by size:
1155 1065 489 428 406 309 105
SacI G_AGCT'C
Cuts at: 0 574 1404 3208 3957
Size: 574 830 1804 749
Fragments arranged by size:
1804 830 749 574
SanDI GG'GwC_CC
Cuts at: 0 535 3957
Size: 535 3422
SapI GCTCTTCn'nnn_
Cuts at: 0 2147 2823 2990 3957
Size: 2147 676 167 967
Fragments arranged by size:
2147 967 676 167
SexAI A'CCwGG_T
Cuts at: 0 122 3957
Size: 122 3835
SfcI C'TryA_G
Cuts at: 0 203 223 465 479 886 1004 1312 1623
Size: 203 20 242 14 407 118 308 311
Cuts at: 1623 2221 2263 2296 2326 2935 2968 3439 3887
Size: 598 42 33 30 609 33 471 448
Cuts at: 3887 3957
Size: 70
Fragments arranged by size:
609 598 471 448 407 311 308 242
203 118 70 42 33 33 30 20
14
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1346 3957
Size: 1346 2611
SfoI GGC'GCC
Cuts at: 0 720 3957
Size: 720 3237
SgrAI Cr'CCGG_yG
Cuts at: 0 1778 3957
Size: 1778 2179
SmaI CCC'GGG
Cuts at: 0 2611 3957
Size: 2611 1346
SmlI C'TyrA_G
Cuts at: 0 1377 2059 2188 3706 3957
Size: 1377 682 129 1518 251
Fragments arranged by size:
1518 1377 682 251 129
StuI AGG'CCT
Cuts at: 0 236 963 1967 2703 3957
Size: 236 727 1004 736 1254
Fragments arranged by size:
1254 1004 736 727 236
StyI C'CwwG_G
Cuts at: 0 50 68 1491 2345 3588 3957
Size: 50 18 1423 854 1243 369
Fragments arranged by size:
1423 1243 854 369 50 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 828 1045 1558 1575 2029 2104 2905 3957
Size: 828 217 513 17 454 75 801 1052
Fragments arranged by size:
1052 828 801 513 454 217 75 17
TatI w'GTAC_w
Cuts at: 0 3788 3866 3957
Size: 3788 78 91
Fragments arranged by size:
3788 91 78
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 824 856 1116 1148 1419 1451 2972 3004
Size: 824 32 260 32 271 32 1521 32
Cuts at: 3004 3957
Size: 953
Fragments arranged by size:
1521 953 824 271 260 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 28 876 1932 3957
Size: 28 848 1056 2025
Fragments arranged by size:
2025 1056 848 28
XmaI C'CCGG_G
Cuts at: 0 2609 3957
Size: 2609 1348
XmnI GAAnn'nnTTC
Cuts at: 0 1284 3957
Size: 1284 2673
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BglI BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
StuI StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AgeI AleI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglII BmgBI BsaBI BsaXI BsiEI BsiWI BsrGI
BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI
EagI EcoRI EcoRV FspAI HindIII HpaI MfeI MluI
NdeI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SgfI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI ZraI