Restriction map for
pF1KF0156
(Linear) (Six-base) MAPSORT of: FLJ00156.seq Check: 1597 from: 1 to: 5574
FLJ00156.orf
With 182 enzymes: SgfI *
September 4, 2009 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 956 5574
Size: 956 4618
AatII G_ACGT'C
Cuts at: 0 4109 5150 5574
Size: 4109 1041 424
Fragments arranged by size:
4109 1041 424
AccI GT'mk_AC
Cuts at: 0 1196 2308 3335 5574
Size: 1196 1112 1027 2239
Fragments arranged by size:
2239 1196 1112 1027
Acc65I G'GTAC_C
Cuts at: 0 296 5574
Size: 296 5278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 381 569 654 1117 1264 1387 1622 3613
Size: 381 188 85 463 147 123 235 1991
Cuts at: 3613 3929 4743 4764 5010 5574
Size: 316 814 21 246 564
Fragments arranged by size:
1991 814 564 463 381 316 246 235
188 147 123 85 21
AhdI GACnn_n'nnGTC
Cuts at: 0 1797 5574
Size: 1797 3777
AleI CACnn'nnGTG
Cuts at: 0 290 964 4552 4912 5092 5421 5574
Size: 290 674 3588 360 180 329 153
Fragments arranged by size:
3588 674 360 329 290 180 153
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 982 5439 5574
Size: 982 4457 135
Fragments arranged by size:
4457 982 135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 5574
Size: 27 5547
AlwNI CAG_nnn'CTG
Cuts at: 0 376 831 1389 1399 1731 1900 1947 2430
Size: 376 455 558 10 332 169 47 483
Cuts at: 2430 2454 4602 4787 5214 5574
Size: 24 2148 185 427 360
Fragments arranged by size:
2148 558 483 455 427 376 360 332
185 169 47 24 10
ApaI G_GGCC'C
Cuts at: 0 1256 5242 5281 5574
Size: 1256 3986 39 293
Fragments arranged by size:
3986 1256 293 39
ApaLI G'TGCA_C
Cuts at: 0 1332 5574
Size: 1332 4242
ApoI r'AATT_y
Cuts at: 0 350 3874 4246 5574
Size: 350 3524 372 1328
Fragments arranged by size:
3524 1328 372 350
AvaI C'yCGr_G
Cuts at: 0 2671 4950 5574
Size: 2671 2279 624
Fragments arranged by size:
2671 2279 624
BamHI G'GATC_C
Cuts at: 0 1045 2831 5574
Size: 1045 1786 2743
Fragments arranged by size:
2743 1786 1045
BanI G'GyrC_C
Cuts at: 0 296 1298 1342 1408 4470 5155 5574
Size: 296 1002 44 66 3062 685 419
Fragments arranged by size:
3062 1002 685 419 296 66 44
BanII G_rGCy'C
Cuts at: 0 458 488 765 1256 1497 1545 1700 2294
Size: 458 30 277 491 241 48 155 594
Cuts at: 2294 2980 3852 4931 5242 5281 5464 5574
Size: 686 872 1079 311 39 183 110
Fragments arranged by size:
1079 872 686 594 491 458 311 277
241 183 155 110 48 39 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 420 2316 2348 2369 2410 3155 3525 4705
Size: 420 1896 32 21 41 745 370 1180
Cuts at: 4705 5350 5574
Size: 645 224
Fragments arranged by size:
1896 1180 745 645 420 370 224 41
32 21
BbvCI CC'TCA_GC
Cuts at: 0 43 658 1450 1666 1883 2065 2652 3068
Size: 43 615 792 216 217 182 587 416
Cuts at: 3068 4405 4603 5432 5574
Size: 1337 198 829 142
Fragments arranged by size:
1337 829 792 615 587 416 217 216
198 182 142 43
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4941 4975 5574
Size: 4941 34 599
Fragments arranged by size:
4941 599 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 5074 5574
Size: 5074 500
BclI T'GATC_A
Cuts at: 0 3012 5574
Size: 3012 2562
BfrBI ATG'CAT
Cuts at: 0 2238 2761 5574
Size: 2238 523 2813
Fragments arranged by size:
2813 2238 523
BglI GCCn_nnn'nGGC
Cuts at: 0 562 2676 4565 4955 5574
Size: 562 2114 1889 390 619
Fragments arranged by size:
2114 1889 619 562 390
BglII A'GATC_T
Cuts at: 0 623 746 3497 5574
Size: 623 123 2751 2077
Fragments arranged by size:
2751 2077 623 123
BlpI GC'TnA_GC
Cuts at: 0 487 4930 5574
Size: 487 4443 644
Fragments arranged by size:
4443 644 487
Bme1580I G_kGCm'C
Cuts at: 0 1256 1336 1419 4475 4873 5242 5281 5574
Size: 1256 80 83 3056 398 369 39 293
Fragments arranged by size:
3056 1256 398 369 293 83 80 39
BmgBI CAC'GTC
Cuts at: 0 1179 2346 4038 4209 5574
Size: 1179 1167 1692 171 1365
Fragments arranged by size:
1692 1365 1179 1167 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 919 2190 3751 5460 5574
Size: 919 1271 1561 1709 114
Fragments arranged by size:
1709 1561 1271 919 114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2058 4101 5574
Size: 2058 2043 1473
Fragments arranged by size:
2058 2043 1473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 502 853 1303 1492 1612 1698 1745
Size: 114 388 351 450 189 120 86 47
Cuts at: 1745 1759 1771 2032 2476 2513 2934 3514 4071
Size: 14 12 261 444 37 421 580 557
Cuts at: 4071 5574
Size: 1503
Fragments arranged by size:
1503 580 557 450 444 421 388 351
261 189 120 114 86 47 37 14
12
Bpu10I CC'TnA_GC
Cuts at: 0 43 195 658 688 1118 1450 1666 1883
Size: 43 152 463 30 430 332 216 217
Cuts at: 1883 2065 2652 2974 2979 3068 4405 4603 5432
Size: 182 587 322 5 89 1337 198 829
Cuts at: 5432 5458 5574
Size: 26 116
Fragments arranged by size:
1337 829 587 463 430 332 322 217
216 198 182 152 116 89 43 30
26 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1710 2533 5492 5574
Size: 703 1007 823 2959 82
Fragments arranged by size:
2959 1007 823 703 82
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 1630 5574
Size: 77 1553 3944
Fragments arranged by size:
3944 1553 77
BsaAI yAC'GTr
Cuts at: 0 4073 5003 5094 5574
Size: 4073 930 91 480
Fragments arranged by size:
4073 930 480 91
BsaBI GATnn'nnATC
Cuts at: 0 5305 5574
Size: 5305 269
BsaHI Gr'CG_yC
Cuts at: 0 4106 5147 5574
Size: 4106 1041 427
Fragments arranged by size:
4106 1041 427
BsaWI w'CCGG_w
Cuts at: 0 3983 5574
Size: 3983 1591
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1945 1975 3303 3333 5574
Size: 1945 30 1328 30 2241
Fragments arranged by size:
2241 1945 1328 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1342 1675 1990 2551 3942 4730 5574
Size: 1342 333 315 561 1391 788 844
Fragments arranged by size:
1391 1342 844 788 561 333 315
BseYI C'CCAG_C
Cuts at: 0 390 863 1324 1482 2120 2856 3104 3120
Size: 390 473 461 158 638 736 248 16
Cuts at: 3120 3586 3724 4500 4763 5280 5574
Size: 466 138 776 263 517 294
Fragments arranged by size:
776 736 638 517 473 466 461 390
294 263 248 158 138 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1061 1913 2431 3084 3124 3262 3411
Size: 223 838 852 518 653 40 138 149
Cuts at: 3411 4231 4243 5040 5574
Size: 820 12 797 534
Fragments arranged by size:
852 838 820 797 653 534 518 223
149 138 40 12
BsiHKAI G_wGCw'C
Cuts at: 0 458 488 1336 1700 1748 2018 2180 2980
Size: 458 30 848 364 48 270 162 800
Cuts at: 2980 4072 4931 5181 5464 5574
Size: 1092 859 250 283 110
Fragments arranged by size:
1092 859 848 800 458 364 283 270
250 162 110 48 30
BsmI GAATG_Cn'
Cuts at: 0 549 2143 5574
Size: 549 1594 3431
Fragments arranged by size:
3431 1594 549
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1212 1862 2533 4199 5154 5574
Size: 1212 650 671 1666 955 420
Fragments arranged by size:
1666 1212 955 671 650 420
Bsp1286I G_dGCh'C
Cuts at: 0 458 488 765 1256 1336 1419 1497 1545
Size: 458 30 277 491 80 83 78 48
Cuts at: 1545 1700 1748 2018 2180 2294 2980 3852 4072
Size: 155 48 270 162 114 686 872 220
Cuts at: 4072 4475 4873 4931 5181 5242 5281 5464 5574
Size: 403 398 58 250 61 39 183 110
Fragments arranged by size:
872 686 491 458 403 398 277 270
250 220 183 162 155 114 110 83
80 78 61 58 48 48 39 30
BspEI T'CCGG_A
Cuts at: 0 3983 5574
Size: 3983 1591
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 956 1384 3742 4145 5574
Size: 956 428 2358 403 1429
Fragments arranged by size:
2358 1429 956 428 403
BsrBI CCG'CTC
Cuts at: 0 2529 5042 5574
Size: 2529 2513 532
Fragments arranged by size:
2529 2513 532
BsrDI GCAATG_nn'
Cuts at: 0 2238 3744 5574
Size: 2238 1506 1830
Fragments arranged by size:
2238 1830 1506
BsrGI T'GTAC_A
Cuts at: 0 710 1961 5574
Size: 710 1251 3613
Fragments arranged by size:
3613 1251 710
BssSI C'ACGA_G
Cuts at: 0 3420 5574
Size: 3420 2154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1731 1900 4357 5574
Size: 1731 169 2457 1217
Fragments arranged by size:
2457 1731 1217 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 150 180 799 969 3966 4911 5574
Size: 150 30 619 170 2997 945 663
Fragments arranged by size:
2997 945 663 619 170 150 30
BstYI r'GATC_y
Cuts at: 0 623 746 1045 2831 3497 3828 5073 5574
Size: 623 123 299 1786 666 331 1245 501
Fragments arranged by size:
1786 1245 666 623 501 331 299 123
Bsu36I CC'TnA_GG
Cuts at: 0 319 1589 5574
Size: 319 1270 3985
Fragments arranged by size:
3985 1270 319
BtgI C'CryG_G
Cuts at: 0 2899 3323 3719 3842 5554 5574
Size: 2899 424 396 123 1712 20
Fragments arranged by size:
2899 1712 424 396 123 20
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1873 3471 5574
Size: 1873 1598 2103
Fragments arranged by size:
2103 1873 1598
BtsI GCAGTG_nn'
Cuts at: 0 1111 1732 3348 4358 5574
Size: 1111 621 1616 1010 1216
Fragments arranged by size:
1616 1216 1111 1010 621
ClaI AT'CG_AT
Cuts at: 0 2474 5574
Size: 2474 3100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 618 653 5574
Size: 618 35 4921
Fragments arranged by size:
4921 618 35
DraI TTT'AAA
Cuts at: 0 2087 3564 3891 5574
Size: 2087 1477 327 1683
Fragments arranged by size:
2087 1683 1477 327
DraIII CAC_nnn'GTG
Cuts at: 0 2633 3255 4062 5574
Size: 2633 622 807 1512
Fragments arranged by size:
2633 1512 807 622
DrdI GACnn_nn'nnGTC
Cuts at: 0 3922 5329 5574
Size: 3922 1407 245
Fragments arranged by size:
3922 1407 245
EaeI y'GGCC_r
Cuts at: 0 57 1430 1517 3279 3956 4566 4677 5216
Size: 57 1373 87 1762 677 610 111 539
Cuts at: 5216 5557 5574
Size: 341 17
Fragments arranged by size:
1762 1373 677 610 539 341 111 87
57 17
EarI CTCTTCn'nnn_
Cuts at: 0 887 1093 2008 2049 2510 2736 4060 5386
Size: 887 206 915 41 461 226 1324 1326
Cuts at: 5386 5574
Size: 188
Fragments arranged by size:
1326 1324 915 887 461 226 206 188
41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 4040 5574
Size: 4040 1534
EcoICRI GAG'CTC
Cuts at: 0 456 486 1698 2978 4929 5462 5574
Size: 456 30 1212 1280 1951 533 112
Fragments arranged by size:
1951 1280 1212 533 456 112 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 381 502 569 654 853 1117 1264
Size: 114 267 121 67 85 199 264 147
Cuts at: 1264 1303 1387 1492 1612 1622 1698 1745 1759
Size: 39 84 105 120 10 76 47 14
Cuts at: 1759 1771 2032 2476 2513 2934 3514 3613 3929
Size: 12 261 444 37 421 580 99 316
Cuts at: 3929 4071 4743 4764 5010 5574
Size: 142 672 21 246 564
Fragments arranged by size:
672 580 564 444 421 316 267 264
261 246 199 147 142 121 120 114
105 99 85 84 76 67 47 39
37 21 14 12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2826 3478 4969 5272 5574
Size: 2826 652 1491 303 302
Fragments arranged by size:
2826 1491 652 303 302
EcoO109I rG'GnC_Cy
Cuts at: 0 535 913 1479 1806 2688 2970 3267 3771
Size: 535 378 566 327 882 282 297 504
Cuts at: 3771 4236 4269 4607 4754 4815 5238 5277 5454
Size: 465 33 338 147 61 423 39 177
Cuts at: 5454 5487 5516 5574
Size: 33 29 58
Fragments arranged by size:
882 566 535 504 465 423 378 338
327 297 282 177 147 61 58 39
33 33 29
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 603 1282 1809 2116 2344 3214 5574
Size: 603 679 527 307 228 870 2360
Fragments arranged by size:
2360 870 679 603 527 307 228
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1783 1815 2569 2601 2701 2733 3403 3435
Size: 1783 32 754 32 100 32 670 32
Cuts at: 3435 3772 3804 5574
Size: 337 32 1770
Fragments arranged by size:
1783 1770 754 670 337 100 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 3567 4029 5574
Size: 3567 462 1545
Fragments arranged by size:
3567 1545 462
KpnI G_GTAC'C
Cuts at: 0 300 5574
Size: 300 5274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2234 2412 3383 3606 4832 5574
Size: 2234 178 971 223 1226 742
Fragments arranged by size:
2234 1226 971 742 223 178
MscI TGG'CCA
Cuts at: 0 59 1519 3281 4568 4679 5218 5574
Size: 59 1460 1762 1287 111 539 356
Fragments arranged by size:
1762 1460 1287 539 356 111 59
MslI CAynn'nnrTG
Cuts at: 0 31 290 950 964 2023 2183 3964 4552
Size: 31 259 660 14 1059 160 1781 588
Cuts at: 4552 4912 5092 5421 5574
Size: 360 180 329 153
Fragments arranged by size:
1781 1059 660 588 360 329 259 180
160 153 31 14
MspA1I CmG'CkG
Cuts at: 0 360 657 1600 2430 3108 3721 4763 4784
Size: 360 297 943 830 678 613 1042 21
Cuts at: 4784 4853 5574
Size: 69 721
Fragments arranged by size:
1042 943 830 721 678 613 360 297
69 21
NcoI C'CATG_G
Cuts at: 0 2899 3842 5554 5574
Size: 2899 943 1712 20
Fragments arranged by size:
2899 1712 943 20
NsiI A_TGCA'T
Cuts at: 0 2240 2763 5574
Size: 2240 523 2811
Fragments arranged by size:
2811 2240 523
NspI r_CATG'y
Cuts at: 0 955 3450 3750 4185 5574
Size: 955 2495 300 435 1389
Fragments arranged by size:
2495 1389 955 435 300
PasI CC'CwG_GG
Cuts at: 0 1536 3767 4223 5484 5574
Size: 1536 2231 456 1261 90
Fragments arranged by size:
2231 1536 1261 456 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 306 507 831 1389 1515 1727 1947 2822
Size: 306 201 324 558 126 212 220 875
Cuts at: 2822 5234 5574
Size: 2412 340
Fragments arranged by size:
2412 875 558 340 324 306 220 212
201 126
PfoI T'CCnGG_A
Cuts at: 0 1355 1748 2009 2834 3824 5574
Size: 1355 393 261 825 990 1750
Fragments arranged by size:
1750 1355 990 825 393 261
PmlI CAC'GTG
Cuts at: 0 4073 5003 5094 5574
Size: 4073 930 91 480
Fragments arranged by size:
4073 930 480 91
PpuMI rG'GwC_Cy
Cuts at: 0 535 1479 1806 2688 3267 3771 4236 4754
Size: 535 944 327 882 579 504 465 518
Cuts at: 4754 4815 5574
Size: 61 759
Fragments arranged by size:
944 882 759 579 535 518 504 465
327 61
PspOMI G'GGCC_C
Cuts at: 0 1252 5238 5277 5574
Size: 1252 3986 39 297
Fragments arranged by size:
3986 1252 297 39
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3745 3777 5574
Size: 3745 32 1797
Fragments arranged by size:
3745 1797 32
PstI C_TGCA'G
Cuts at: 0 474 941 1226 1295 1445 2174 3482 3665
Size: 474 467 285 69 150 729 1308 183
Cuts at: 3665 4022 4515 4598 5381 5541 5574
Size: 357 493 83 783 160 33
Fragments arranged by size:
1308 783 729 493 474 467 357 285
183 160 150 83 69 33
PvuII CAG'CTG
Cuts at: 0 360 657 1600 2430 3108 4763 4784 5574
Size: 360 297 943 830 678 1655 21 790
Fragments arranged by size:
1655 943 830 790 678 360 297 21
SacI G_AGCT'C
Cuts at: 0 458 488 1700 2980 4931 5464 5574
Size: 458 30 1212 1280 1951 533 110
Fragments arranged by size:
1951 1280 1212 533 458 110 30
SacII CC_GC'GG
Cuts at: 0 3722 5574
Size: 3722 1852
SanDI GG'GwC_CC
Cuts at: 0 4236 5574
Size: 4236 1338
SapI GCTCTTCn'nnn_
Cuts at: 0 1093 4060 5574
Size: 1093 2967 1514
Fragments arranged by size:
2967 1514 1093
SbfI CC_TGCA'GG
Cuts at: 0 941 3482 5574
Size: 941 2541 2092
Fragments arranged by size:
2541 2092 941
SexAI A'CCwGG_T
Cuts at: 0 3251 3977 5574
Size: 3251 726 1597
Fragments arranged by size:
3251 1597 726
SfcI C'TryA_G
Cuts at: 0 470 937 1222 1291 1441 2170 2733 3478
Size: 470 467 285 69 150 729 563 745
Cuts at: 3478 3661 4018 4511 4587 4594 4712 5377 5469
Size: 183 357 493 76 7 118 665 92
Cuts at: 5469 5537 5574
Size: 68 37
Fragments arranged by size:
745 729 665 563 493 470 467 357
285 183 150 118 92 76 69 68
37 7
SmaI CCC'GGG
Cuts at: 0 2673 5574
Size: 2673 2901
SmlI C'TyrA_G
Cuts at: 0 718 1689 2548 5507 5574
Size: 718 971 859 2959 67
Fragments arranged by size:
2959 971 859 718 67
SphI G_CATG'C
Cuts at: 0 955 4185 5574
Size: 955 3230 1389
Fragments arranged by size:
3230 1389 955
SrfI GCCC'GGGC
Cuts at: 0 2673 5574
Size: 2673 2901
StuI AGG'CCT
Cuts at: 0 331 2554 3559 4006 4975 5574
Size: 331 2223 1005 447 969 599
Fragments arranged by size:
2223 1005 969 599 447 331
StyI C'CwwG_G
Cuts at: 0 526 1514 1640 2684 2899 3842 3857 5554
Size: 526 988 126 1044 215 943 15 1697
Cuts at: 5554 5574
Size: 20
Fragments arranged by size:
1697 1044 988 943 526 215 126 20
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 234 691 1041 1954 3118 3958 4015 4514
Size: 234 457 350 913 1164 840 57 499
Cuts at: 4514 4586 5104 5574
Size: 72 518 470
Fragments arranged by size:
1164 913 840 518 499 470 457 350
234 72 57
TatI w'GTAC_w
Cuts at: 0 710 989 1961 5574
Size: 710 279 972 3613
Fragments arranged by size:
3613 972 710 279
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1037 1069 1409 1441 1471 1503 1569 1601
Size: 1037 32 340 32 30 32 66 32
Cuts at: 1601 2680 2712 3303 3335 3340 3372 5574
Size: 1079 32 591 32 5 32 2202
Fragments arranged by size:
2202 1079 1037 591 340 66 32 32
32 32 32 32 32 30 5
Tth111I GACn'n_nGTC
Cuts at: 0 2059 4285 4813 5574
Size: 2059 2226 528 761
Fragments arranged by size:
2226 2059 761 528
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1552 1840 3764 5434 5574
Size: 1552 288 1924 1670 140
Fragments arranged by size:
1924 1670 1552 288 140
XmaI C'CCGG_G
Cuts at: 0 2671 5574
Size: 2671 2903
XmnI GAAnn'nnTTC
Cuts at: 0 515 3820 5574
Size: 515 3305 1754
Fragments arranged by size:
3305 1754 515
ZraI GAC'GTC
Cuts at: 0 4107 5148 5574
Size: 4107 1041 426
Fragments arranged by size:
4107 1041 426
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I HindIII KpnI MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PsrI PstI PvuII SacI SacII SanDI SapI SbfI
SexAI SfcI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AscI AseI AsiSI
AvrII BaeI BbeI BmtI BsiEI BsiWI BspHI BsrFI
BssHII BstBI BstEII BstZ17I EagI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HpaI KasI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PpiI PshAI PsiI PspXI PvuI RsrII
SalI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI