(Linear) (Six-base) MAPSORT of: FLJ00162.seq Check: 5603 from: 1 to: 2115 FLJ00162.orf With 182 enzymes: SgfI * March 3, 2008 11:13 .. AccI GT'mk_AC Cuts at: 0 1262 2115 Size: 1262 853 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 574 1858 2115 Size: 20 554 1284 257 Fragments arranged by size: 1284 554 257 20 AflIII A'CryG_T Cuts at: 0 1106 1146 2115 Size: 1106 40 969 Fragments arranged by size: 1106 969 40 AhdI GACnn_n'nnGTC Cuts at: 0 1749 2115 Size: 1749 366 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 452 2115 Size: 452 1663 ApaI G_GGCC'C Cuts at: 0 1400 2115 Size: 1400 715 BanI G'GyrC_C Cuts at: 0 1100 1966 2115 Size: 1100 866 149 Fragments arranged by size: 1100 866 149 BanII G_rGCy'C Cuts at: 0 910 1117 1400 1490 1649 1787 2115 Size: 910 207 283 90 159 138 328 Fragments arranged by size: 910 328 283 207 159 138 90 BbsI GAAGACnn'nnnn_ Cuts at: 0 1552 2115 Size: 1552 563 BbvCI CC'TCA_GC Cuts at: 0 1696 2115 Size: 1696 419 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1990 2024 2115 Size: 1990 34 91 Fragments arranged by size: 1990 91 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1363 2115 Size: 1363 752 BglI GCCn_nnn'nGGC Cuts at: 0 1849 2115 Size: 1849 266 BglII A'GATC_T Cuts at: 0 1214 1976 2115 Size: 1214 762 139 Fragments arranged by size: 1214 762 139 BlpI GC'TnA_GC Cuts at: 0 214 2115 Size: 214 1901 Bme1580I G_kGCm'C Cuts at: 0 845 1400 2115 Size: 845 555 715 Fragments arranged by size: 845 715 555 BmgBI CAC'GTC Cuts at: 0 600 1147 2040 2115 Size: 600 547 893 75 Fragments arranged by size: 893 600 547 75 BmrI ACTGGGnnnn_n' Cuts at: 0 549 1123 1536 2115 Size: 549 574 413 579 Fragments arranged by size: 579 574 549 413 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 150 481 2115 Size: 150 331 1634 Fragments arranged by size: 1634 331 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 163 327 894 1321 1645 1786 1969 2115 Size: 163 164 567 427 324 141 183 146 Fragments arranged by size: 567 427 324 183 164 163 146 141 Bpu10I CC'TnA_GC Cuts at: 0 1696 2115 Size: 1696 419 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 458 2115 Size: 458 1657 BsaI GGTCTCn'nnnn_ Cuts at: 0 19 2115 Size: 19 2096 BsaHI Gr'CG_yC Cuts at: 0 758 2115 Size: 758 1357 BsaWI w'CCGG_w Cuts at: 0 98 1208 2115 Size: 98 1110 907 Fragments arranged by size: 1110 907 98 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 160 218 345 1054 1684 2115 Size: 37 123 58 127 709 630 431 Fragments arranged by size: 709 630 431 127 123 58 37 BseYI C'CCAG_C Cuts at: 0 676 1715 2115 Size: 676 1039 400 Fragments arranged by size: 1039 676 400 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 2115 Size: 412 1703 BsiHKAI G_wGCw'C Cuts at: 0 656 674 1490 1787 2115 Size: 656 18 816 297 328 Fragments arranged by size: 816 656 328 297 18 BsmBI CGTCTCn'nnnn_ Cuts at: 0 339 2115 Size: 339 1776 Bsp1286I G_dGCh'C Cuts at: 0 656 674 845 910 1117 1400 1490 1649 Size: 656 18 171 65 207 283 90 159 Cuts at: 1649 1787 2115 Size: 138 328 Fragments arranged by size: 656 328 283 207 171 159 138 90 65 18 BspEI T'CCGG_A Cuts at: 0 98 1208 2115 Size: 98 1110 907 Fragments arranged by size: 1110 907 98 BspHI T'CATG_A Cuts at: 0 890 2115 Size: 890 1225 BsrBI CCG'CTC Cuts at: 0 1564 2115 Size: 1564 551 BsrDI GCAATG_nn' Cuts at: 0 2006 2115 Size: 2006 109 BsrFI r'CCGG_y Cuts at: 0 1789 1824 2115 Size: 1789 35 291 Fragments arranged by size: 1789 291 35 BstAPI GCAn_nnn'nTGC Cuts at: 0 978 1334 2115 Size: 978 356 781 Fragments arranged by size: 978 781 356 BstXI CCAn_nnnn'nTGG Cuts at: 0 381 808 1136 2115 Size: 381 427 328 979 Fragments arranged by size: 979 427 381 328 BstYI r'GATC_y Cuts at: 0 833 1214 1976 2115 Size: 833 381 762 139 Fragments arranged by size: 833 762 381 139 Bsu36I CC'TnA_GG Cuts at: 0 1961 2115 Size: 1961 154 BtgI C'CryG_G Cuts at: 0 847 923 1155 1599 1631 1667 2115 Size: 847 76 232 444 32 36 448 Fragments arranged by size: 847 448 444 232 76 36 32 BtsI GCAGTG_nn' Cuts at: 0 120 129 252 2115 Size: 120 9 123 1863 Fragments arranged by size: 1863 123 120 9 DraIII CAC_nnn'GTG Cuts at: 0 1131 1147 2115 Size: 1131 16 968 Fragments arranged by size: 1131 968 16 DrdI GACnn_nn'nnGTC Cuts at: 0 523 2115 Size: 523 1592 EaeI y'GGCC_r Cuts at: 0 1457 1664 1814 1826 2115 Size: 1457 207 150 12 289 Fragments arranged by size: 1457 289 207 150 12 EarI CTCTTCn'nnn_ Cuts at: 0 47 1246 1443 2021 2115 Size: 47 1199 197 578 94 Fragments arranged by size: 1199 578 197 94 47 EcoICRI GAG'CTC Cuts at: 0 1488 1785 2115 Size: 1488 297 330 Fragments arranged by size: 1488 330 297 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 20 163 327 574 894 1321 1645 1786 Size: 20 143 164 247 320 427 324 141 Cuts at: 1786 1858 1969 2115 Size: 72 111 146 Fragments arranged by size: 427 324 320 247 164 146 143 141 111 72 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 342 430 1069 1924 1948 2115 Size: 342 88 639 855 24 167 Fragments arranged by size: 855 639 342 167 88 24 EcoO109I rG'GnC_Cy Cuts at: 0 1396 1397 2115 Size: 1396 1 718 Fragments arranged by size: 1396 718 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 981 2115 Size: 981 1134 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 16 48 1735 1767 2029 2061 2115 Size: 16 32 1687 32 262 32 54 Fragments arranged by size: 1687 262 54 32 32 32 16 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 383 1964 2115 Size: 383 1581 151 Fragments arranged by size: 1581 383 151 MscI TGG'CCA Cuts at: 0 1666 2115 Size: 1666 449 MslI CAynn'nnrTG Cuts at: 0 940 1021 1027 1165 1336 1660 2011 2115 Size: 940 81 6 138 171 324 351 104 Fragments arranged by size: 940 351 324 171 138 104 81 6 MspA1I CmG'CkG Cuts at: 0 204 262 489 587 1836 2115 Size: 204 58 227 98 1249 279 Fragments arranged by size: 1249 279 227 204 98 58 NcoI C'CATG_G Cuts at: 0 1599 2115 Size: 1599 516 NspI r_CATG'y Cuts at: 0 981 1110 2115 Size: 981 129 1005 Fragments arranged by size: 1005 981 129 PasI CC'CwG_GG Cuts at: 0 220 2115 Size: 220 1895 PciI A'CATG_T Cuts at: 0 1106 2115 Size: 1106 1009 PflMI CCAn_nnn'nTGG Cuts at: 0 812 1131 1600 1740 2115 Size: 812 319 469 140 375 Fragments arranged by size: 812 469 375 319 140 PfoI T'CCnGG_A Cuts at: 0 343 910 1361 2115 Size: 343 567 451 754 Fragments arranged by size: 754 567 451 343 PshAI GACnn'nnGTC Cuts at: 0 1929 2115 Size: 1929 186 PsiI TTA'TAA Cuts at: 0 107 2115 Size: 107 2008 PspOMI G'GGCC_C Cuts at: 0 1396 2115 Size: 1396 719 PstI C_TGCA'G Cuts at: 0 331 2115 Size: 331 1784 PvuII CAG'CTG Cuts at: 0 262 489 587 2115 Size: 262 227 98 1528 Fragments arranged by size: 1528 262 227 98 SacI G_AGCT'C Cuts at: 0 1490 1787 2115 Size: 1490 297 328 Fragments arranged by size: 1490 328 297 SapI GCTCTTCn'nnn_ Cuts at: 0 2021 2115 Size: 2021 94 SfcI C'TryA_G Cuts at: 0 327 2115 Size: 327 1788 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1849 2115 Size: 1849 266 SmlI C'TyrA_G Cuts at: 0 473 2115 Size: 473 1642 SphI G_CATG'C Cuts at: 0 981 2115 Size: 981 1134 StyI C'CwwG_G Cuts at: 0 236 1599 2115 Size: 236 1363 516 Fragments arranged by size: 1363 516 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1142 1728 2115 Size: 1142 586 387 Fragments arranged by size: 1142 586 387 TatI w'GTAC_w Cuts at: 0 686 2115 Size: 686 1429 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 839 871 2115 Size: 839 32 1244 Fragments arranged by size: 1244 839 32 Tth111I GACn'n_nGTC Cuts at: 0 1551 2115 Size: 1551 564 XbaI T'CTAG_A Cuts at: 0 2034 2115 Size: 2034 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1410 2115 Size: 1410 705 Enzymes that do cut: AccI AcuI AflIII AhdI AlfI ApaI BanI BanII BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PshAI PsiI PspOMI PstI PvuII SacI SapI SfcI SfiI SmlI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BclI BfrBI BmtI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EciI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PpiI PpuMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XhoI XmaI XmnI ZraI