Restriction map for pF1KF0162
 (Linear) (Six-base) MAPSORT of: FLJ00162.seq  Check: 5603  from: 1  to: 2115



FLJ00162.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:13  ..

AccI GT'mk_AC

Cuts at:      0    1262    2115 
   Size:       1262     853
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     574    1858    2115 
   Size:         20     554    1284     257

  Fragments arranged by size: 

                1284     554     257      20
 
AflIII A'CryG_T

Cuts at:      0    1106    1146    2115 
   Size:       1106      40     969

  Fragments arranged by size: 

                1106     969      40
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1749    2115 
   Size:       1749     366
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     452    2115 
   Size:        452    1663
 
ApaI G_GGCC'C

Cuts at:      0    1400    2115 
   Size:       1400     715
 
BanI G'GyrC_C

Cuts at:      0    1100    1966    2115 
   Size:       1100     866     149

  Fragments arranged by size: 

                1100     866     149
 
BanII G_rGCy'C

Cuts at:      0     910    1117    1400    1490    1649    1787    2115 
   Size:        910     207     283      90     159     138     328

  Fragments arranged by size: 

                 910     328     283     207     159     138      90
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1552    2115 
   Size:       1552     563
 
BbvCI CC'TCA_GC

Cuts at:      0    1696    2115 
   Size:       1696     419
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1990    2024    2115 
   Size:       1990      34      91

  Fragments arranged by size: 

                1990      91      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1363    2115 
   Size:       1363     752
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1849    2115 
   Size:       1849     266
 
BglII A'GATC_T

Cuts at:      0    1214    1976    2115 
   Size:       1214     762     139

  Fragments arranged by size: 

                1214     762     139
 
BlpI GC'TnA_GC

Cuts at:      0     214    2115 
   Size:        214    1901
 
Bme1580I G_kGCm'C

Cuts at:      0     845    1400    2115 
   Size:        845     555     715

  Fragments arranged by size: 

                 845     715     555
 
BmgBI CAC'GTC

Cuts at:      0     600    1147    2040    2115 
   Size:        600     547     893      75

  Fragments arranged by size: 

                 893     600     547      75
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     549    1123    1536    2115 
   Size:        549     574     413     579

  Fragments arranged by size: 

                 579     574     549     413
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     150     481    2115 
   Size:        150     331    1634

  Fragments arranged by size: 

                1634     331     150
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     327     894    1321    1645    1786    1969    2115 
   Size:        163     164     567     427     324     141     183     146

  Fragments arranged by size: 

                 567     427     324     183     164     163     146     141
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1696    2115 
   Size:       1696     419
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     458    2115 
   Size:        458    1657
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      19    2115 
   Size:         19    2096
 
BsaHI Gr'CG_yC

Cuts at:      0     758    2115 
   Size:        758    1357
 
BsaWI w'CCGG_w

Cuts at:      0      98    1208    2115 
   Size:         98    1110     907

  Fragments arranged by size: 

                1110     907      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     160     218     345    1054    1684    2115 
   Size:         37     123      58     127     709     630     431

  Fragments arranged by size: 

                 709     630     431     127     123      58      37
 
BseYI C'CCAG_C

Cuts at:      0     676    1715    2115 
   Size:        676    1039     400

  Fragments arranged by size: 

                1039     676     400
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412    2115 
   Size:        412    1703
 
BsiHKAI G_wGCw'C

Cuts at:      0     656     674    1490    1787    2115 
   Size:        656      18     816     297     328

  Fragments arranged by size: 

                 816     656     328     297      18
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     339    2115 
   Size:        339    1776
 
Bsp1286I G_dGCh'C

Cuts at:      0     656     674     845     910    1117    1400    1490    1649 
   Size:        656      18     171      65     207     283      90     159

Cuts at:   1649    1787    2115 
   Size:        138     328

  Fragments arranged by size: 

                 656     328     283     207     171     159     138      90
                  65      18
 
BspEI T'CCGG_A

Cuts at:      0      98    1208    2115 
   Size:         98    1110     907

  Fragments arranged by size: 

                1110     907      98
 
BspHI T'CATG_A

Cuts at:      0     890    2115 
   Size:        890    1225
 
BsrBI CCG'CTC

Cuts at:      0    1564    2115 
   Size:       1564     551
 
BsrDI GCAATG_nn'

Cuts at:      0    2006    2115 
   Size:       2006     109
 
BsrFI r'CCGG_y

Cuts at:      0    1789    1824    2115 
   Size:       1789      35     291

  Fragments arranged by size: 

                1789     291      35
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     978    1334    2115 
   Size:        978     356     781

  Fragments arranged by size: 

                 978     781     356
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     381     808    1136    2115 
   Size:        381     427     328     979

  Fragments arranged by size: 

                 979     427     381     328
 
BstYI r'GATC_y

Cuts at:      0     833    1214    1976    2115 
   Size:        833     381     762     139

  Fragments arranged by size: 

                 833     762     381     139
 
Bsu36I CC'TnA_GG

Cuts at:      0    1961    2115 
   Size:       1961     154
 
BtgI C'CryG_G

Cuts at:      0     847     923    1155    1599    1631    1667    2115 
   Size:        847      76     232     444      32      36     448

  Fragments arranged by size: 

                 847     448     444     232      76      36      32
 
BtsI GCAGTG_nn'

Cuts at:      0     120     129     252    2115 
   Size:        120       9     123    1863

  Fragments arranged by size: 

                1863     123     120       9
 
DraIII CAC_nnn'GTG

Cuts at:      0    1131    1147    2115 
   Size:       1131      16     968

  Fragments arranged by size: 

                1131     968      16
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     523    2115 
   Size:        523    1592
 
EaeI y'GGCC_r

Cuts at:      0    1457    1664    1814    1826    2115 
   Size:       1457     207     150      12     289

  Fragments arranged by size: 

                1457     289     207     150      12
 
EarI CTCTTCn'nnn_

Cuts at:      0      47    1246    1443    2021    2115 
   Size:         47    1199     197     578      94

  Fragments arranged by size: 

                1199     578     197      94      47
 
EcoICRI GAG'CTC

Cuts at:      0    1488    1785    2115 
   Size:       1488     297     330

  Fragments arranged by size: 

                1488     330     297
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     163     327     574     894    1321    1645    1786 
   Size:         20     143     164     247     320     427     324     141

Cuts at:   1786    1858    1969    2115 
   Size:         72     111     146

  Fragments arranged by size: 

                 427     324     320     247     164     146     143     141
                 111      72      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     342     430    1069    1924    1948    2115 
   Size:        342      88     639     855      24     167

  Fragments arranged by size: 

                 855     639     342     167      88      24
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1396    1397    2115 
   Size:       1396       1     718

  Fragments arranged by size: 

                1396     718       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     981    2115 
   Size:        981    1134
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48    1735    1767    2029    2061    2115 
   Size:         16      32    1687      32     262      32      54

  Fragments arranged by size: 

                1687     262      54      32      32      32      16
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     383    1964    2115 
   Size:        383    1581     151

  Fragments arranged by size: 

                1581     383     151
 
MscI TGG'CCA

Cuts at:      0    1666    2115 
   Size:       1666     449
 
MslI CAynn'nnrTG

Cuts at:      0     940    1021    1027    1165    1336    1660    2011    2115 
   Size:        940      81       6     138     171     324     351     104

  Fragments arranged by size: 

                 940     351     324     171     138     104      81       6
            
 
MspA1I CmG'CkG

Cuts at:      0     204     262     489     587    1836    2115 
   Size:        204      58     227      98    1249     279

  Fragments arranged by size: 

                1249     279     227     204      98      58
 
NcoI C'CATG_G

Cuts at:      0    1599    2115 
   Size:       1599     516
 
NspI r_CATG'y

Cuts at:      0     981    1110    2115 
   Size:        981     129    1005

  Fragments arranged by size: 

                1005     981     129
 
PasI CC'CwG_GG

Cuts at:      0     220    2115 
   Size:        220    1895
 
PciI A'CATG_T

Cuts at:      0    1106    2115 
   Size:       1106    1009
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     812    1131    1600    1740    2115 
   Size:        812     319     469     140     375

  Fragments arranged by size: 

                 812     469     375     319     140
 
PfoI T'CCnGG_A

Cuts at:      0     343     910    1361    2115 
   Size:        343     567     451     754

  Fragments arranged by size: 

                 754     567     451     343
 
PshAI GACnn'nnGTC

Cuts at:      0    1929    2115 
   Size:       1929     186
 
PsiI TTA'TAA

Cuts at:      0     107    2115 
   Size:        107    2008
 
PspOMI G'GGCC_C

Cuts at:      0    1396    2115 
   Size:       1396     719
 
PstI C_TGCA'G

Cuts at:      0     331    2115 
   Size:        331    1784
 
PvuII CAG'CTG

Cuts at:      0     262     489     587    2115 
   Size:        262     227      98    1528

  Fragments arranged by size: 

                1528     262     227      98
 
SacI G_AGCT'C

Cuts at:      0    1490    1787    2115 
   Size:       1490     297     328

  Fragments arranged by size: 

                1490     328     297
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2021    2115 
   Size:       2021      94
 
SfcI C'TryA_G

Cuts at:      0     327    2115 
   Size:        327    1788
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1849    2115 
   Size:       1849     266
 
SmlI C'TyrA_G

Cuts at:      0     473    2115 
   Size:        473    1642
 
SphI G_CATG'C

Cuts at:      0     981    2115 
   Size:        981    1134
 
StyI C'CwwG_G

Cuts at:      0     236    1599    2115 
   Size:        236    1363     516

  Fragments arranged by size: 

                1363     516     236
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1142    1728    2115 
   Size:       1142     586     387

  Fragments arranged by size: 

                1142     586     387
 
TatI w'GTAC_w

Cuts at:      0     686    2115 
   Size:        686    1429
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     839     871    2115 
   Size:        839      32    1244

  Fragments arranged by size: 

                1244     839      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1551    2115 
   Size:       1551     564
 
XbaI T'CTAG_A

Cuts at:      0    2034    2115 
   Size:       2034      81
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1410    2115 
   Size:       1410     705
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AlfI     ApaI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I
     BtgI     BtsI   DraIII     DrdI     EaeI     EarI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I     MmeI     MscI     MslI   MspA1I
     NcoI     NspI     PasI     PciI    PflMI     PfoI    PshAI     PsiI
   PspOMI     PstI    PvuII     SacI     SapI     SfcI     SfiI     SmlI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BclI    BfrBI     BmtI    BsaAI
    BsaBI    BsaXI    BsiEI    BsiWI     BsmI    BspMI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI     DraI
     EagI     EciI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PmeI     PmlI     PpiI    PpuMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI
     XhoI     XmaI     XmnI     ZraI
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