Restriction map for
pF1KSDF0166
(Linear) (Six-base) MAPSORT of: FLJ00166.seq Check: 9665 from: 1 to: 1317
FLJ00166.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 261 1278 1317
Size: 261 1017 39
Fragments arranged by size:
1017 261 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 890 1317
Size: 698 192 427
Fragments arranged by size:
698 427 192
AflII C'TTAA_G
Cuts at: 0 498 1317
Size: 498 819
ApaI G_GGCC'C
Cuts at: 0 133 1317
Size: 133 1184
ApoI r'AATT_y
Cuts at: 0 196 412 790 861 1317
Size: 196 216 378 71 456
Fragments arranged by size:
456 378 216 196 71
AseI AT'TA_AT
Cuts at: 0 269 1317
Size: 269 1048
AvaI C'yCGr_G
Cuts at: 0 125 1317
Size: 125 1192
BamHI G'GATC_C
Cuts at: 0 531 1317
Size: 531 786
BanII G_rGCy'C
Cuts at: 0 133 1317
Size: 133 1184
BbsI GAAGACnn'nnnn_
Cuts at: 0 281 1317
Size: 281 1036
Bme1580I G_kGCm'C
Cuts at: 0 133 1317
Size: 133 1184
BmtI G_CTAG'C
Cuts at: 0 610 1317
Size: 610 707
BsaHI Gr'CG_yC
Cuts at: 0 963 1317
Size: 963 354
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1178 1317
Size: 1178 139
BseYI C'CCAG_C
Cuts at: 0 140 1317
Size: 140 1177
BsiEI CG_ry'CG
Cuts at: 0 113 1317
Size: 113 1204
Bsp1286I G_dGCh'C
Cuts at: 0 133 1317
Size: 133 1184
BspHI T'CATG_A
Cuts at: 0 455 1317
Size: 455 862
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 261 1278 1317
Size: 261 1017 39
Fragments arranged by size:
1017 261 39
BssSI C'ACGA_G
Cuts at: 0 1037 1317
Size: 1037 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 1051 1258 1317
Size: 150 901 207 59
Fragments arranged by size:
901 207 150 59
BstYI r'GATC_y
Cuts at: 0 216 531 1317
Size: 216 315 786
Fragments arranged by size:
786 315 216
BtgI C'CryG_G
Cuts at: 0 36 525 1317
Size: 36 489 792
Fragments arranged by size:
792 489 36
DraI TTT'AAA
Cuts at: 0 923 954 1196 1245 1317
Size: 923 31 242 49 72
Fragments arranged by size:
923 242 72 49 31
EaeI y'GGCC_r
Cuts at: 0 110 1317
Size: 110 1207
EagI C'GGCC_G
Cuts at: 0 110 1317
Size: 110 1207
EarI CTCTTCn'nnn_
Cuts at: 0 163 633 638 674 1317
Size: 163 470 5 36 643
Fragments arranged by size:
643 470 163 36 5
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 698 890 1317
Size: 698 192 427
Fragments arranged by size:
698 427 192
EcoO109I rG'GnC_Cy
Cuts at: 0 9 129 1317
Size: 9 120 1188
Fragments arranged by size:
1188 120 9
EcoRI G'AATT_C
Cuts at: 0 412 790 1317
Size: 412 378 527
Fragments arranged by size:
527 412 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 523 555 560 592 1317
Size: 523 32 5 32 725
Fragments arranged by size:
725 523 32 32 5
HincII GTy'rAC
Cuts at: 0 177 945 1317
Size: 177 768 372
Fragments arranged by size:
768 372 177
HindIII A'AGCT_T
Cuts at: 0 995 1025 1220 1317
Size: 995 30 195 97
Fragments arranged by size:
995 195 97 30
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 939 1138 1317
Size: 939 199 179
Fragments arranged by size:
939 199 179
MslI CAynn'nnrTG
Cuts at: 0 971 1124 1317
Size: 971 153 193
Fragments arranged by size:
971 193 153
NcoI C'CATG_G
Cuts at: 0 525 1317
Size: 525 792
NheI G'CTAG_C
Cuts at: 0 606 1317
Size: 606 711
PfoI T'CCnGG_A
Cuts at: 0 235 777 1317
Size: 235 542 540
Fragments arranged by size:
542 540 235
PpuMI rG'GwC_Cy
Cuts at: 0 9 1317
Size: 9 1308
PspOMI G'GGCC_C
Cuts at: 0 129 1317
Size: 129 1188
SapI GCTCTTCn'nnn_
Cuts at: 0 633 674 1317
Size: 633 41 643
Fragments arranged by size:
643 633 41
SfcI C'TryA_G
Cuts at: 0 696 1317
Size: 696 621
SmaI CCC'GGG
Cuts at: 0 127 1317
Size: 127 1190
SmlI C'TyrA_G
Cuts at: 0 498 1317
Size: 498 819
StuI AGG'CCT
Cuts at: 0 848 1317
Size: 848 469
StyI C'CwwG_G
Cuts at: 0 525 761 1317
Size: 525 236 556
Fragments arranged by size:
556 525 236
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 953 985 1317
Size: 953 32 332
Fragments arranged by size:
953 332 32
XbaI T'CTAG_A
Cuts at: 0 798 1317
Size: 798 519
XmaI C'CCGG_G
Cuts at: 0 125 1317
Size: 125 1192
XmnI GAAnn'nnTTC
Cuts at: 0 399 683 794 1317
Size: 399 284 111 523
Fragments arranged by size:
523 399 284 111
Enzymes that do cut:
AarI AcuI AflII ApaI ApoI AseI AvaI BamHI
BanII BbsI Bme1580I BmtI BsaHI BseRI BseYI BsiEI
Bsp1286I BspHI BspMI BssSI BstAPI BstYI BtgI DraI
EaeI EagI EarI Eco57MI EcoO109I EcoRI Hin4I HincII
HindIII MmeI MslI NcoI NheI PfoI PpuMI PspOMI
SapI SfcI SmaI SmlI StuI StyI TstI XbaI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI Tth111I XcmI
XhoI ZraI