Restriction map for
pF1KSDF0183
(Linear) (Six-base) MAPSORT of: FLJ00183.seq Check: 7833 from: 1 to: 678
FLJ00183.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 276 297 678
Size: 276 21 381
Fragments arranged by size:
381 276 21
AatII G_ACGT'C
Cuts at: 0 431 678
Size: 431 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 678
Size: 595 83
AvaI C'yCGr_G
Cuts at: 0 66 678
Size: 66 612
BanI G'GyrC_C
Cuts at: 0 61 678
Size: 61 617
BanII G_rGCy'C
Cuts at: 0 251 678
Size: 251 427
BbeI G_GCGC'C
Cuts at: 0 65 678
Size: 65 613
BciVI GTATCCnnnnn_n'
Cuts at: 0 277 678
Size: 277 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 678
Size: 214 464
BsaHI Gr'CG_yC
Cuts at: 0 62 428 678
Size: 62 366 250
Fragments arranged by size:
366 250 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 678
Size: 352 30 296
Fragments arranged by size:
352 296 30
BsiEI CG_ry'CG
Cuts at: 0 59 263 678
Size: 59 204 415
Fragments arranged by size:
415 204 59
BsiHKAI G_wGCw'C
Cuts at: 0 137 251 678
Size: 137 114 427
Fragments arranged by size:
427 137 114
Bsp1286I G_dGCh'C
Cuts at: 0 137 251 678
Size: 137 114 427
Fragments arranged by size:
427 137 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 276 297 678
Size: 276 21 381
Fragments arranged by size:
381 276 21
BsrBI CCG'CTC
Cuts at: 0 200 678
Size: 200 478
BsrFI r'CCGG_y
Cuts at: 0 58 262 678
Size: 58 204 416
Fragments arranged by size:
416 204 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 102 678
Size: 102 576
BstYI r'GATC_y
Cuts at: 0 70 678
Size: 70 608
Bsu36I CC'TnA_GG
Cuts at: 0 87 678
Size: 87 591
BtgI C'CryG_G
Cuts at: 0 47 293 356 678
Size: 47 246 63 322
Fragments arranged by size:
322 246 63 47
DraI TTT'AAA
Cuts at: 0 515 678
Size: 515 163
EaeI y'GGCC_r
Cuts at: 0 56 260 290 678
Size: 56 204 30 388
Fragments arranged by size:
388 204 56 30
EagI C'GGCC_G
Cuts at: 0 56 260 678
Size: 56 204 418
Fragments arranged by size:
418 204 56
EarI CTCTTCn'nnn_
Cuts at: 0 398 678
Size: 398 280
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 265 550 678
Size: 265 285 128
Fragments arranged by size:
285 265 128
EcoICRI GAG'CTC
Cuts at: 0 249 678
Size: 249 429
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 678
Size: 595 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 283 678
Size: 283 395
EcoO109I rG'GnC_Cy
Cuts at: 0 408 678
Size: 408 270
HaeII r_GCGC'y
Cuts at: 0 65 678
Size: 65 613
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 533 565 678
Size: 352 32 149 32 113
Fragments arranged by size:
352 149 113 32 32
KasI G'GCGC_C
Cuts at: 0 61 678
Size: 61 617
MscI TGG'CCA
Cuts at: 0 292 678
Size: 292 386
MslI CAynn'nnrTG
Cuts at: 0 436 678
Size: 436 242
MspA1I CmG'CkG
Cuts at: 0 49 55 321 459 678
Size: 49 6 266 138 219
Fragments arranged by size:
266 219 138 49 6
NaeI GCC'GGC
Cuts at: 0 60 678
Size: 60 618
NarI GG'CG_CC
Cuts at: 0 62 678
Size: 62 616
NcoI C'CATG_G
Cuts at: 0 293 356 678
Size: 293 63 322
Fragments arranged by size:
322 293 63
NgoMIV G'CCGG_C
Cuts at: 0 58 678
Size: 58 620
NspI r_CATG'y
Cuts at: 0 367 678
Size: 367 311
PflMI CCAn_nnn'nTGG
Cuts at: 0 642 678
Size: 642 36
PsiI TTA'TAA
Cuts at: 0 529 678
Size: 529 149
PstI C_TGCA'G
Cuts at: 0 33 678
Size: 33 645
PvuII CAG'CTG
Cuts at: 0 459 678
Size: 459 219
SacI G_AGCT'C
Cuts at: 0 251 678
Size: 251 427
SacII CC_GC'GG
Cuts at: 0 50 678
Size: 50 628
SexAI A'CCwGG_T
Cuts at: 0 641 678
Size: 641 37
SfcI C'TryA_G
Cuts at: 0 29 678
Size: 29 649
SfoI GGC'GCC
Cuts at: 0 63 678
Size: 63 615
SmaI CCC'GGG
Cuts at: 0 68 678
Size: 68 610
StyI C'CwwG_G
Cuts at: 0 143 293 356 678
Size: 143 150 63 322
Fragments arranged by size:
322 150 143 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 238 678
Size: 238 440
TatI w'GTAC_w
Cuts at: 0 122 678
Size: 122 556
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 376 408 678
Size: 376 32 270
Fragments arranged by size:
376 270 32
XmaI C'CCGG_G
Cuts at: 0 66 678
Size: 66 612
ZraI GAC'GTC
Cuts at: 0 429 678
Size: 429 249
Enzymes that do cut:
AarI AatII AcuI AvaI BanI BanII BbeI BciVI
BsaI BsaHI BsaXI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstAPI BstYI Bsu36I BtgI DraI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PsiI PstI PvuII SacI SacII SexAI
SfcI SfoI SmaI StyI TaqII TatI TstI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI
BseRI BseYI BsgI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraIII DrdI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI