Restriction map for
pF1KF0229
(Linear) (Six-base) MAPSORT of: FLJ00229.seq Check: 2050 from: 1 to: 1824
FLJ00229.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 680 750 1824
Size: 680 70 1074
Fragments arranged by size:
1074 680 70
AccI GT'mk_AC
Cuts at: 0 1664 1824
Size: 1664 160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 948 1186 1824
Size: 948 238 638
Fragments arranged by size:
948 638 238
AflIII A'CryG_T
Cuts at: 0 712 1824
Size: 712 1112
AlwNI CAG_nnn'CTG
Cuts at: 0 922 1824
Size: 922 902
ApaI G_GGCC'C
Cuts at: 0 16 32 1824
Size: 16 16 1792
Fragments arranged by size:
1792 16 16
ApoI r'AATT_y
Cuts at: 0 312 1003 1136 1441 1536 1824
Size: 312 691 133 305 95 288
Fragments arranged by size:
691 312 305 288 133 95
AscI GG'CGCG_CC
Cuts at: 0 110 1824
Size: 110 1714
BamHI G'GATC_C
Cuts at: 0 435 1634 1824
Size: 435 1199 190
Fragments arranged by size:
1199 435 190
BanI G'GyrC_C
Cuts at: 0 84 894 1824
Size: 84 810 930
Fragments arranged by size:
930 810 84
BanII G_rGCy'C
Cuts at: 0 16 32 881 1824
Size: 16 16 849 943
Fragments arranged by size:
943 849 16 16
BbeI G_GCGC'C
Cuts at: 0 88 898 1824
Size: 88 810 926
Fragments arranged by size:
926 810 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 353 1824
Size: 353 1471
BbvCI CC'TCA_GC
Cuts at: 0 248 1472 1824
Size: 248 1224 352
Fragments arranged by size:
1224 352 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1687 1721 1824
Size: 1687 34 103
Fragments arranged by size:
1687 103 34
BclI T'GATC_A
Cuts at: 0 524 1824
Size: 524 1300
BglI GCCn_nnn'nGGC
Cuts at: 0 20 97 1824
Size: 20 77 1727
Fragments arranged by size:
1727 77 20
Bme1580I G_kGCm'C
Cuts at: 0 16 32 1824
Size: 16 16 1792
Fragments arranged by size:
1792 16 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 643 823 1824
Size: 330 313 180 1001
Fragments arranged by size:
1001 330 313 180
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1430 1824
Size: 1430 394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1069 1217 1824
Size: 235 834 148 607
Fragments arranged by size:
834 607 235 148
Bpu10I CC'TnA_GC
Cuts at: 0 248 1142 1472 1824
Size: 248 894 330 352
Fragments arranged by size:
894 352 330 248
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1477 1824
Size: 1477 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 1109 1824
Size: 1109 715
BsaBI GATnn'nnATC
Cuts at: 0 632 1824
Size: 632 1192
BsaHI Gr'CG_yC
Cuts at: 0 85 895 1824
Size: 85 810 929
Fragments arranged by size:
929 810 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 157 187 1017 1047 1824
Size: 157 30 830 30 777
Fragments arranged by size:
830 777 157 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 384 1824
Size: 384 1440
BseYI C'CCAG_C
Cuts at: 0 161 219 1824
Size: 161 58 1605
Fragments arranged by size:
1605 161 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 766 1824
Size: 169 597 1058
Fragments arranged by size:
1058 597 169
BsiHKAI G_wGCw'C
Cuts at: 0 566 1715 1824
Size: 566 1149 109
Fragments arranged by size:
1149 566 109
BsmI GAATG_Cn'
Cuts at: 0 252 1824
Size: 252 1572
Bsp1286I G_dGCh'C
Cuts at: 0 16 32 566 881 1715 1824
Size: 16 16 534 315 834 109
Fragments arranged by size:
834 534 315 109 16 16
BspHI T'CATG_A
Cuts at: 0 717 798 993 1824
Size: 717 81 195 831
Fragments arranged by size:
831 717 195 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 680 750 1137 1824
Size: 680 70 387 687
Fragments arranged by size:
687 680 387 70
BsrDI GCAATG_nn'
Cuts at: 0 1777 1824
Size: 1777 47
BsrFI r'CCGG_y
Cuts at: 0 87 1514 1824
Size: 87 1427 310
Fragments arranged by size:
1427 310 87
BsrGI T'GTAC_A
Cuts at: 0 1266 1824
Size: 1266 558
BssHII G'CGCG_C
Cuts at: 0 110 1824
Size: 110 1714
BstBI TT'CG_AA
Cuts at: 0 1107 1824
Size: 1107 717
BstYI r'GATC_y
Cuts at: 0 435 1634 1824
Size: 435 1199 190
Fragments arranged by size:
1199 435 190
BtgI C'CryG_G
Cuts at: 0 141 230 1399 1824
Size: 141 89 1169 425
Fragments arranged by size:
1169 425 141 89
BtsI GCAGTG_nn'
Cuts at: 0 186 1824
Size: 186 1638
ClaI AT'CG_AT
Cuts at: 0 636 1824
Size: 636 1188
DraI TTT'AAA
Cuts at: 0 1226 1824
Size: 1226 598
DraIII CAC_nnn'GTG
Cuts at: 0 192 561 1824
Size: 192 369 1263
Fragments arranged by size:
1263 369 192
EaeI y'GGCC_r
Cuts at: 0 138 144 1093 1824
Size: 138 6 949 731
Fragments arranged by size:
949 731 138 6
EarI CTCTTCn'nnn_
Cuts at: 0 122 1232 1399 1610 1824
Size: 122 1110 167 211 214
Fragments arranged by size:
1110 214 211 167 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 165 1025 1824
Size: 165 860 799
Fragments arranged by size:
860 799 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 948 1069 1186 1217 1824
Size: 235 713 121 117 31 607
Fragments arranged by size:
713 607 235 121 117 31
EcoO109I rG'GnC_Cy
Cuts at: 0 12 13 28 150 1468 1824
Size: 12 1 15 122 1318 356
Fragments arranged by size:
1318 356 122 15 12 1
EcoRI G'AATT_C
Cuts at: 0 1003 1824
Size: 1003 821
HaeII r_GCGC'y
Cuts at: 0 88 898 1824
Size: 88 810 926
Fragments arranged by size:
926 810 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 1824
Size: 157 32 1635
Fragments arranged by size:
1635 157 32
HincII GTy'rAC
Cuts at: 0 752 939 1824
Size: 752 187 885
Fragments arranged by size:
885 752 187
HindIII A'AGCT_T
Cuts at: 0 1368 1824
Size: 1368 456
HpaI GTT'AAC
Cuts at: 0 752 939 1824
Size: 752 187 885
Fragments arranged by size:
885 752 187
KasI G'GCGC_C
Cuts at: 0 84 894 1824
Size: 84 810 930
Fragments arranged by size:
930 810 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 492 999 1824
Size: 29 463 507 825
Fragments arranged by size:
825 507 463 29
MscI TGG'CCA
Cuts at: 0 1095 1824
Size: 1095 729
MslI CAynn'nnrTG
Cuts at: 0 685 998 1760 1824
Size: 685 313 762 64
Fragments arranged by size:
762 685 313 64
NaeI GCC'GGC
Cuts at: 0 89 1824
Size: 89 1735
NarI GG'CG_CC
Cuts at: 0 85 895 1824
Size: 85 810 929
Fragments arranged by size:
929 810 85
NcoI C'CATG_G
Cuts at: 0 1399 1824
Size: 1399 425
NgoMIV G'CCGG_C
Cuts at: 0 87 1824
Size: 87 1737
NspI r_CATG'y
Cuts at: 0 716 1824
Size: 716 1108
PciI A'CATG_T
Cuts at: 0 712 1824
Size: 712 1112
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 1584 1824
Size: 231 1353 240
Fragments arranged by size:
1353 240 231
PfoI T'CCnGG_A
Cuts at: 0 392 1824
Size: 392 1432
PpuMI rG'GwC_Cy
Cuts at: 0 1468 1824
Size: 1468 356
PspOMI G'GGCC_C
Cuts at: 0 12 28 1824
Size: 12 16 1796
Fragments arranged by size:
1796 16 12
ScaI AGT'ACT
Cuts at: 0 961 1824
Size: 961 863
SfcI C'TryA_G
Cuts at: 0 1127 1824
Size: 1127 697
SfoI GGC'GCC
Cuts at: 0 86 896 1824
Size: 86 810 928
Fragments arranged by size:
928 810 86
SmlI C'TyrA_G
Cuts at: 0 1456 1824
Size: 1456 368
SspI AAT'ATT
Cuts at: 0 285 1824
Size: 285 1539
StyI C'CwwG_G
Cuts at: 0 515 1399 1824
Size: 515 884 425
Fragments arranged by size:
884 515 425
SwaI ATTT'AAAT
Cuts at: 0 1226 1824
Size: 1226 598
TatI w'GTAC_w
Cuts at: 0 959 1266 1824
Size: 959 307 558
Fragments arranged by size:
959 558 307
Tth111I GACn'n_nGTC
Cuts at: 0 1669 1824
Size: 1669 155
XbaI T'CTAG_A
Cuts at: 0 1483 1599 1824
Size: 1483 116 225
Fragments arranged by size:
1483 225 116
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI ApoI AscI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI
BstYI BtgI BtsI ClaI DraI DraIII EaeI EarI
EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII
HpaI KasI MmeI MscI MslI NaeI NarI NcoI
NgoMIV NspI PciI PflMI PfoI PpuMI PspOMI ScaI
SfcI SfoI SmlI SspI StyI SwaI TatI Tth111I
XbaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AseI AsiSI AvaI AvrII BaeI
BciVI BfrBI BglII BlpI BmgBI BmtI BsaAI BsaWI
BsiEI BsiWI BsmBI BspEI BsrBI BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgZI CspCI DrdI EagI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI KpnI MfeI
MluI MspA1I NdeI NheI NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI TaqII TstI XcmI XhoI
XmaI XmnI ZraI