Restriction map for
pF1KSDF0258
(Linear) (Six-base) MAPSORT of: FLJ00258.seq Check: 974 from: 1 to: 2304
FLJ00258.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AatII G_ACGT'C
Cuts at: 0 1237 2304
Size: 1237 1067
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 1165 1339 1422 1969 2083 2304
Size: 943 222 174 83 547 114 221
Fragments arranged by size:
943 547 222 221 174 114 83
AfeI AGC'GCT
Cuts at: 0 2086 2304
Size: 2086 218
AflII C'TTAA_G
Cuts at: 0 523 2304
Size: 523 1781
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1653 2304
Size: 1653 651
AlwNI CAG_nnn'CTG
Cuts at: 0 18 129 744 1051 2100 2304
Size: 18 111 615 307 1049 204
Fragments arranged by size:
1049 615 307 204 111 18
ApaI G_GGCC'C
Cuts at: 0 197 1421 1728 2304
Size: 197 1224 307 576
Fragments arranged by size:
1224 576 307 197
ApoI r'AATT_y
Cuts at: 0 223 2304
Size: 223 2081
AvaI C'yCGr_G
Cuts at: 0 189 1407 1510 1661 1708 2304
Size: 189 1218 103 151 47 596
Fragments arranged by size:
1218 596 189 151 103 47
BamHI G'GATC_C
Cuts at: 0 1442 2304
Size: 1442 862
BanI G'GyrC_C
Cuts at: 0 1310 2304
Size: 1310 994
BanII G_rGCy'C
Cuts at: 0 41 197 278 313 532 604 1153 1421
Size: 41 156 81 35 219 72 549 268
Cuts at: 1421 1537 1661 1709 1728 2126 2236 2304
Size: 116 124 48 19 398 110 68
Fragments arranged by size:
549 398 268 219 156 124 116 110
81 72 68 48 41 35 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 1216 2304
Size: 1216 1088
BbvCI CC'TCA_GC
Cuts at: 0 76 962 2221 2304
Size: 76 886 1259 83
Fragments arranged by size:
1259 886 83 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1834 1868 2304
Size: 1834 34 436
Fragments arranged by size:
1834 436 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 789 2304
Size: 789 1515
BclI T'GATC_A
Cuts at: 0 1107 2304
Size: 1107 1197
BlpI GC'TnA_GC
Cuts at: 0 52 2304
Size: 52 2252
Bme1580I G_kGCm'C
Cuts at: 0 197 821 1421 1728 2304
Size: 197 624 600 307 576
Fragments arranged by size:
624 600 576 307 197
BmrI ACTGGGnnnn_n'
Cuts at: 0 982 2304
Size: 982 1322
BmtI G_CTAG'C
Cuts at: 0 304 2304
Size: 304 2000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 654 1551 2304
Size: 654 897 753
Fragments arranged by size:
897 753 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1521 1791 1906 2077 2304
Size: 40 1481 270 115 171 227
Fragments arranged by size:
1481 270 227 171 115 40
Bpu10I CC'TnA_GC
Cuts at: 0 76 296 962 1655 2221 2304
Size: 76 220 666 693 566 83
Fragments arranged by size:
693 666 566 220 83 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 159 1093 1576 2304
Size: 159 934 483 728
Fragments arranged by size:
934 728 483 159
BsaAI yAC'GTr
Cuts at: 0 816 1572 2304
Size: 816 756 732
Fragments arranged by size:
816 756 732
BsaHI Gr'CG_yC
Cuts at: 0 1234 2304
Size: 1234 1070
BsaWI w'CCGG_w
Cuts at: 0 1548 2304
Size: 1548 756
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 742 772 959 989 1732 1762
Size: 412 30 300 30 187 30 743 30
Cuts at: 1762 2304
Size: 542
Fragments arranged by size:
743 542 412 300 187 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 598 601 646 928 1261 1264 1717 1737
Size: 598 3 45 282 333 3 453 20
Cuts at: 1737 1861 2053 2244 2304
Size: 124 192 191 60
Fragments arranged by size:
598 453 333 282 192 191 124 60
45 20 3 3
BseYI C'CCAG_C
Cuts at: 0 196 277 1606 2304
Size: 196 81 1329 698
Fragments arranged by size:
1329 698 196 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1543 2304
Size: 1543 761
BsiEI CG_ry'CG
Cuts at: 0 332 2304
Size: 332 1972
BsiHKAI G_wGCw'C
Cuts at: 0 41 532 2304
Size: 41 491 1772
Fragments arranged by size:
1772 491 41
BsmI GAATG_Cn'
Cuts at: 0 664 2304
Size: 664 1640
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1894 2304
Size: 1894 410
Bsp1286I G_dGCh'C
Cuts at: 0 41 197 278 313 532 604 821 1153
Size: 41 156 81 35 219 72 217 332
Cuts at: 1153 1421 1537 1661 1709 1728 2126 2236 2304
Size: 268 116 124 48 19 398 110 68
Fragments arranged by size:
398 332 268 219 217 156 124 116
110 81 72 68 48 41 35 19
BspEI T'CCGG_A
Cuts at: 0 1548 2304
Size: 1548 756
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 344 808 1235 1367 1773 2304
Size: 344 464 427 132 406 531
Fragments arranged by size:
531 464 427 406 344 132
BsrBI CCG'CTC
Cuts at: 0 348 2304
Size: 348 1956
BsrDI GCAATG_nn'
Cuts at: 0 557 2304
Size: 557 1747
BsrFI r'CCGG_y
Cuts at: 0 830 1454 2304
Size: 830 624 850
Fragments arranged by size:
850 830 624
BssSI C'ACGA_G
Cuts at: 0 67 844 2304
Size: 67 777 1460
Fragments arranged by size:
1460 777 67
BstAPI GCAn_nnn'nTGC
Cuts at: 0 129 849 2304
Size: 129 720 1455
Fragments arranged by size:
1455 720 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 204 648 2304
Size: 204 444 1656
Fragments arranged by size:
1656 444 204
BstYI r'GATC_y
Cuts at: 0 982 1442 1633 2304
Size: 982 460 191 671
Fragments arranged by size:
982 671 460 191
Bsu36I CC'TnA_GG
Cuts at: 0 368 2304
Size: 368 1936
BtgI C'CryG_G
Cuts at: 0 107 2003 2304
Size: 107 1896 301
Fragments arranged by size:
1896 301 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1365 2304
Size: 1365 939
BtsI GCAGTG_nn'
Cuts at: 0 709 889 1761 2304
Size: 709 180 872 543
Fragments arranged by size:
872 709 543 180
DraIII CAC_nnn'GTG
Cuts at: 0 651 2304
Size: 651 1653
DrdI GACnn_nn'nnGTC
Cuts at: 0 1783 2304
Size: 1783 521
EaeI y'GGCC_r
Cuts at: 0 104 329 1089 1217 1466 2006 2304
Size: 104 225 760 128 249 540 298
Fragments arranged by size:
760 540 298 249 225 128 104
EagI C'GGCC_G
Cuts at: 0 329 2304
Size: 329 1975
EarI CTCTTCn'nnn_
Cuts at: 0 377 575 592 1120 1933 2221 2304
Size: 377 198 17 528 813 288 83
Fragments arranged by size:
813 528 377 288 198 83 17
EcoICRI GAG'CTC
Cuts at: 0 39 530 2304
Size: 39 491 1774
Fragments arranged by size:
1774 491 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 943 1165 1339 1422 1521 1791 1906
Size: 40 903 222 174 83 99 270 115
Cuts at: 1906 1969 2077 2083 2304
Size: 63 108 6 221
Fragments arranged by size:
903 270 222 221 174 115 108 99
83 63 40 6
EcoO109I rG'GnC_Cy
Cuts at: 0 193 387 400 975 1418 1724 2304
Size: 193 194 13 575 443 306 580
Fragments arranged by size:
580 575 443 306 194 193 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 406 2103 2304
Size: 406 1697 201
Fragments arranged by size:
1697 406 201
FspI TGC'GCA
Cuts at: 0 1450 2304
Size: 1450 854
HaeII r_GCGC'y
Cuts at: 0 1558 1716 2046 2088 2304
Size: 1558 158 330 42 216
Fragments arranged by size:
1558 330 216 158 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 959 974 991 1006 1092 1124
Size: 172 32 755 15 17 15 86 32
Cuts at: 1124 2304
Size: 1180
Fragments arranged by size:
1180 755 172 86 32 32 17 15
15
HincII GTy'rAC
Cuts at: 0 2205 2304
Size: 2205 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 986 2276 2304
Size: 986 1290 28
Fragments arranged by size:
1290 986 28
MscI TGG'CCA
Cuts at: 0 1468 2008 2304
Size: 1468 540 296
Fragments arranged by size:
1468 540 296
MslI CAynn'nnrTG
Cuts at: 0 814 2304
Size: 814 1490
MspA1I CmG'CkG
Cuts at: 0 27 717 2304
Size: 27 690 1587
Fragments arranged by size:
1587 690 27
NaeI GCC'GGC
Cuts at: 0 832 2304
Size: 832 1472
NgoMIV G'CCGG_C
Cuts at: 0 830 2304
Size: 830 1474
NheI G'CTAG_C
Cuts at: 0 300 2304
Size: 300 2004
NspI r_CATG'y
Cuts at: 0 258 2304
Size: 258 2046
PasI CC'CwG_GG
Cuts at: 0 861 2118 2304
Size: 861 1257 186
Fragments arranged by size:
1257 861 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 1773 2285 2304
Size: 18 1755 512 19
Fragments arranged by size:
1755 512 19 18
PfoI T'CCnGG_A
Cuts at: 0 59 2304
Size: 59 2245
PpuMI rG'GwC_Cy
Cuts at: 0 400 975 2304
Size: 400 575 1329
Fragments arranged by size:
1329 575 400
PshAI GACnn'nnGTC
Cuts at: 0 1499 2304
Size: 1499 805
PspOMI G'GGCC_C
Cuts at: 0 193 1417 1724 2304
Size: 193 1224 307 580
Fragments arranged by size:
1224 580 307 193
PstI C_TGCA'G
Cuts at: 0 125 134 445 805 896 1232 1756 2304
Size: 125 9 311 360 91 336 524 548
Fragments arranged by size:
548 524 360 336 311 125 91 9
PvuII CAG'CTG
Cuts at: 0 27 717 2304
Size: 27 690 1587
Fragments arranged by size:
1587 690 27
SacI G_AGCT'C
Cuts at: 0 41 532 2304
Size: 41 491 1772
Fragments arranged by size:
1772 491 41
SanDI GG'GwC_CC
Cuts at: 0 400 2304
Size: 400 1904
SapI GCTCTTCn'nnn_
Cuts at: 0 592 1120 2304
Size: 592 528 1184
Fragments arranged by size:
1184 592 528
SexAI A'CCwGG_T
Cuts at: 0 647 2304
Size: 647 1657
SfcI C'TryA_G
Cuts at: 0 121 130 441 801 892 1228 1752 2269
Size: 121 9 311 360 91 336 524 517
Cuts at: 2269 2304
Size: 35
Fragments arranged by size:
524 517 360 336 311 121 91 35
9
SmaI CCC'GGG
Cuts at: 0 1409 2304
Size: 1409 895
SmlI C'TyrA_G
Cuts at: 0 523 2304
Size: 523 1781
StuI AGG'CCT
Cuts at: 0 637 2304
Size: 637 1667
StyI C'CwwG_G
Cuts at: 0 761 821 1086 2256 2279 2304
Size: 761 60 265 1170 23 25
Fragments arranged by size:
1170 761 265 60 25 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 874 1051 1057 1442 2304
Size: 20 854 177 6 385 862
Fragments arranged by size:
862 854 385 177 20 6
TatI w'GTAC_w
Cuts at: 0 419 2304
Size: 419 1885
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 1732 1764 2008 2040 2304
Size: 1294 32 406 32 244 32 264
Fragments arranged by size:
1294 406 264 244 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1407 2304
Size: 1407 897
XmnI GAAnn'nnTTC
Cuts at: 0 1958 2304
Size: 1958 346
ZraI GAC'GTC
Cuts at: 0 1235 2304
Size: 1235 1069
Enzymes that do cut:
AatII AcuI AfeI AflII AlfI AlwNI ApaI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NgoMIV NheI NspI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI
SanDI SapI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflIII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BfrBI BglI BglII BmgBI BpuEI BsaBI BsiWI BspHI
BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI
EciI EcoNI EcoRI EcoRV FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI