Restriction map for
pF1KSDF0261
(Linear) (Six-base) MAPSORT of: FLJ00261.seq Check: 6099 from: 1 to: 4791
FLJ00261.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 981 1596 4262 4683 4791
Size: 981 615 2666 421 108
Fragments arranged by size:
2666 981 615 421 108
AatII G_ACGT'C
Cuts at: 0 3683 4791
Size: 3683 1108
AccI GT'mk_AC
Cuts at: 0 3788 4791
Size: 3788 1003
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1589 2026 2278 3134 4003 4756 4791
Size: 503 1086 437 252 856 869 753 35
Fragments arranged by size:
1086 869 856 753 503 437 252 35
AflIII A'CryG_T
Cuts at: 0 4240 4791
Size: 4240 551
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3508 4791
Size: 3508 1283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3815 3847 4791
Size: 3815 32 944
Fragments arranged by size:
3815 944 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1663 3117 3199 3342 3840 4734 4791
Size: 1663 1454 82 143 498 894 57
Fragments arranged by size:
1663 1454 894 498 143 82 57
ApaLI G'TGCA_C
Cuts at: 0 4724 4791
Size: 4724 67
ApoI r'AATT_y
Cuts at: 0 55 451 3535 4791
Size: 55 396 3084 1256
Fragments arranged by size:
3084 1256 396 55
AvaI C'yCGr_G
Cuts at: 0 1297 2594 3364 4292 4568 4791
Size: 1297 1297 770 928 276 223
Fragments arranged by size:
1297 1297 928 770 276 223
AvrII C'CTAG_G
Cuts at: 0 2289 4791
Size: 2289 2502
BamHI G'GATC_C
Cuts at: 0 1459 2159 4791
Size: 1459 700 2632
Fragments arranged by size:
2632 1459 700
BanI G'GyrC_C
Cuts at: 0 993 1104 1270 1789 4423 4791
Size: 993 111 166 519 2634 368
Fragments arranged by size:
2634 993 519 368 166 111
BanII G_rGCy'C
Cuts at: 0 263 371 662 878 1364 1402 2834 3148
Size: 263 108 291 216 486 38 1432 314
Cuts at: 3148 4791
Size: 1643
Fragments arranged by size:
1643 1432 486 314 291 263 216 108
38
BbeI G_GCGC'C
Cuts at: 0 4427 4791
Size: 4427 364
BbsI GAAGACnn'nnnn_
Cuts at: 0 3518 4791
Size: 3518 1273
BbvCI CC'TCA_GC
Cuts at: 0 8 284 1322 1468 2918 4791
Size: 8 276 1038 146 1450 1873
Fragments arranged by size:
1873 1450 1038 276 146 8
BglI GCCn_nnn'nGGC
Cuts at: 0 335 1899 3021 3113 4027 4791
Size: 335 1564 1122 92 914 764
Fragments arranged by size:
1564 1122 914 764 335 92
Bme1580I G_kGCm'C
Cuts at: 0 1674 4728 4791
Size: 1674 3054 63
Fragments arranged by size:
3054 1674 63
BmgBI CAC'GTC
Cuts at: 0 4605 4791
Size: 4605 186
BmrI ACTGGGnnnn_n'
Cuts at: 0 318 890 1405 1553 1606 4791
Size: 318 572 515 148 53 3185
Fragments arranged by size:
3185 572 515 318 148 53
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 956 4791
Size: 531 425 3835
Fragments arranged by size:
3835 531 425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 1640 1832 2818 3016 3123 3364 3426
Size: 709 931 192 986 198 107 241 62
Cuts at: 3426 3904 3955 4063 4540 4612 4663 4791
Size: 478 51 108 477 72 51 128
Fragments arranged by size:
986 931 709 478 477 241 198 192
128 108 107 72 62 51 51
Bpu10I CC'TnA_GC
Cuts at: 0 8 284 941 1322 1468 2402 2918 4111
Size: 8 276 657 381 146 934 516 1193
Cuts at: 4111 4791
Size: 680
Fragments arranged by size:
1193 934 680 657 516 381 276 146
8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2003 2481 3901 4791
Size: 2003 478 1420 890
Fragments arranged by size:
2003 1420 890 478
BsaI GGTCTCn'nnnn_
Cuts at: 0 102 568 2037 2082 2871 3408 4791
Size: 102 466 1469 45 789 537 1383
Fragments arranged by size:
1469 1383 789 537 466 102 45
BsaAI yAC'GTr
Cuts at: 0 1651 4791
Size: 1651 3140
BsaHI Gr'CG_yC
Cuts at: 0 3004 3680 4424 4791
Size: 3004 676 744 367
Fragments arranged by size:
3004 744 676 367
BsaWI w'CCGG_w
Cuts at: 0 300 2826 4791
Size: 300 2526 1965
Fragments arranged by size:
2526 1965 300
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 342 372 1746 1776 2094 2124 2989 3019
Size: 342 30 1374 30 318 30 865 30
Cuts at: 3019 3337 3367 3953 3983 4791
Size: 318 30 586 30 808
Fragments arranged by size:
1374 865 808 586 342 318 318 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 23 25 99 457 652 706 1194 1207
Size: 23 2 74 358 195 54 488 13
Cuts at: 1207 1238 1429 1520 1544 2171 2208 2267 2595
Size: 31 191 91 24 627 37 59 328
Cuts at: 2595 3264 3396 3993 4024 4111 4326 4519 4791
Size: 669 132 597 31 87 215 193 272
Fragments arranged by size:
669 627 597 488 358 328 272 215
195 193 191 132 91 87 74 59
54 37 31 31 24 23 13 2
BseYI C'CCAG_C
Cuts at: 0 17 1158 1773 2047 2059 2980 3278 4791
Size: 17 1141 615 274 12 921 298 1513
Fragments arranged by size:
1513 1141 921 615 298 274 17 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1631 2974 4240 4708 4791
Size: 1631 1343 1266 468 83
Fragments arranged by size:
1631 1343 1266 468 83
BsiHKAI G_wGCw'C
Cuts at: 0 371 1375 1656 2593 2834 3644 4728 4791
Size: 371 1004 281 937 241 810 1084 63
Fragments arranged by size:
1084 1004 937 810 371 281 241 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 211 3671 4791
Size: 211 3460 1120
Fragments arranged by size:
3460 1120 211
Bsp1286I G_dGCh'C
Cuts at: 0 263 371 662 878 1364 1375 1402 1656
Size: 263 108 291 216 486 11 27 254
Cuts at: 1656 1674 2593 2834 3148 3644 4728 4791
Size: 18 919 241 314 496 1084 63
Fragments arranged by size:
1084 919 496 486 314 291 263 254
241 216 108 63 27 18 11
BspEI T'CCGG_A
Cuts at: 0 300 2826 4791
Size: 300 2526 1965
Fragments arranged by size:
2526 1965 300
BspHI T'CATG_A
Cuts at: 0 1283 4791
Size: 1283 3508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 780 981 1596 1920 2646 3428 3575 3800
Size: 780 201 615 324 726 782 147 225
Cuts at: 3800 4262 4683 4791
Size: 462 421 108
Fragments arranged by size:
782 780 726 615 462 421 324 225
201 147 108
BsrBI CCG'CTC
Cuts at: 0 1225 4149 4791
Size: 1225 2924 642
Fragments arranged by size:
2924 1225 642
BsrDI GCAATG_nn'
Cuts at: 0 2557 4791
Size: 2557 2234
BsrFI r'CCGG_y
Cuts at: 0 3107 4791
Size: 3107 1684
BssSI C'ACGA_G
Cuts at: 0 2237 4791
Size: 2237 2554
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3117 4791
Size: 3117 1674
BstEII G'GTnAC_C
Cuts at: 0 2945 4791
Size: 2945 1846
BstXI CCAn_nnnn'nTGG
Cuts at: 0 371 426 1142 4791
Size: 371 55 716 3649
Fragments arranged by size:
3649 716 371 55
BstYI r'GATC_y
Cuts at: 0 484 1459 2159 2501 3323 4478 4791
Size: 484 975 700 342 822 1155 313
Fragments arranged by size:
1155 975 822 700 484 342 313
Bsu36I CC'TnA_GG
Cuts at: 0 71 635 1238 2879 3140 4791
Size: 71 564 603 1641 261 1651
Fragments arranged by size:
1651 1641 603 564 261 71
BtgI C'CryG_G
Cuts at: 0 2144 2213 3170 3379 3434 3470 4791
Size: 2144 69 957 209 55 36 1321
Fragments arranged by size:
2144 1321 957 209 69 55 36
BtsI GCAGTG_nn'
Cuts at: 0 1172 3060 4635 4791
Size: 1172 1888 1575 156
Fragments arranged by size:
1888 1575 1172 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2540 2575 4791
Size: 2540 35 2216
Fragments arranged by size:
2540 2216 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1416 2051 2642 2656 4791
Size: 1416 635 591 14 2135
Fragments arranged by size:
2135 1416 635 591 14
EaeI y'GGCC_r
Cuts at: 0 457 2229 2351 2699 4490 4791
Size: 457 1772 122 348 1791 301
Fragments arranged by size:
1791 1772 457 348 301 122
EarI CTCTTCn'nnn_
Cuts at: 0 434 500 522 554 617 965 973 995
Size: 434 66 22 32 63 348 8 22
Cuts at: 995 1167 1184 2808 3153 3658 3704 3979 4267
Size: 172 17 1624 345 505 46 275 288
Cuts at: 4267 4447 4791
Size: 180 344
Fragments arranged by size:
1624 505 434 348 345 344 288 275
180 172 66 63 46 32 22 22
17 8
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1027 3769 4791
Size: 1027 2742 1022
Fragments arranged by size:
2742 1027 1022
EcoICRI GAG'CTC
Cuts at: 0 369 2832 4791
Size: 369 2463 1959
Fragments arranged by size:
2463 1959 369
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 709 1589 1640 1832 2026 2278 2818
Size: 503 206 880 51 192 194 252 540
Cuts at: 2818 3016 3123 3134 3364 3426 3904 3955 4003
Size: 198 107 11 230 62 478 51 48
Cuts at: 4003 4063 4540 4612 4663 4756 4791
Size: 60 477 72 51 93 35
Fragments arranged by size:
880 540 503 478 477 252 230 206
198 194 192 107 93 72 62 60
51 51 51 48 35 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1623 2921 2998 3316 3457 4791
Size: 1623 1298 77 318 141 1334
Fragments arranged by size:
1623 1334 1298 318 141 77
EcoO109I rG'GnC_Cy
Cuts at: 0 132 1607 1769 1901 2839 2883 3114 3187
Size: 132 1475 162 132 938 44 231 73
Cuts at: 3187 3396 4791
Size: 209 1395
Fragments arranged by size:
1475 1395 938 231 209 162 132 132
73 44
EcoRI G'AATT_C
Cuts at: 0 55 4791
Size: 55 4736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2341 2657 4791
Size: 2341 316 2134
Fragments arranged by size:
2341 2134 316
FspI TGC'GCA
Cuts at: 0 3121 3478 3571 4791
Size: 3121 357 93 1220
Fragments arranged by size:
3121 1220 357 93
HaeII r_GCGC'y
Cuts at: 0 3689 3909 4427 4791
Size: 3689 220 518 364
Fragments arranged by size:
3689 518 364 220
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 342 374 381 413 1315 1347
Size: 121 32 189 32 7 32 902 32
Cuts at: 1347 1678 1710 1746 1778 2901 2933 2989 3021
Size: 331 32 36 32 1123 32 56 32
Cuts at: 3021 3116 3148 3337 3369 4602 4634 4791
Size: 95 32 189 32 1233 32 157
Fragments arranged by size:
1233 1123 902 331 189 189 157 121
95 56 36 32 32 32 32 32
32 32 32 32 32 32 7
HindIII A'AGCT_T
Cuts at: 0 4468 4791
Size: 4468 323
KasI G'GCGC_C
Cuts at: 0 4423 4791
Size: 4423 368
MluI A'CGCG_T
Cuts at: 0 4240 4791
Size: 4240 551
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3624 3656 3966 4419 4490 4733 4791
Size: 3624 32 310 453 71 243 58
Fragments arranged by size:
3624 453 310 243 71 58 32
MscI TGG'CCA
Cuts at: 0 459 2231 2353 4492 4791
Size: 459 1772 122 2139 299
Fragments arranged by size:
2139 1772 459 299 122
MslI CAynn'nnrTG
Cuts at: 0 2237 3586 4791
Size: 2237 1349 1205
Fragments arranged by size:
2237 1349 1205
MspA1I CmG'CkG
Cuts at: 0 1115 2347 3011 3282 3339 3381 3426 3446
Size: 1115 1232 664 271 57 42 45 20
Cuts at: 3446 4050 4773 4791
Size: 604 723 18
Fragments arranged by size:
1232 1115 723 664 604 271 57 45
42 20 18
NaeI GCC'GGC
Cuts at: 0 3109 4791
Size: 3109 1682
NarI GG'CG_CC
Cuts at: 0 4424 4791
Size: 4424 367
NcoI C'CATG_G
Cuts at: 0 2144 3434 4791
Size: 2144 1290 1357
Fragments arranged by size:
2144 1357 1290
NdeI CA'TA_TG
Cuts at: 0 2606 4791
Size: 2606 2185
NgoMIV G'CCGG_C
Cuts at: 0 3107 4791
Size: 3107 1684
NspI r_CATG'y
Cuts at: 0 2308 4791
Size: 2308 2483
PasI CC'CwG_GG
Cuts at: 0 2072 2938 4216 4791
Size: 2072 866 1278 575
Fragments arranged by size:
2072 1278 866 575
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 2819 4041 4791
Size: 687 2132 1222 750
Fragments arranged by size:
2132 1222 750 687
PfoI T'CCnGG_A
Cuts at: 0 3183 3863 4055 4791
Size: 3183 680 192 736
Fragments arranged by size:
3183 736 680 192
PmlI CAC'GTG
Cuts at: 0 1651 4791
Size: 1651 3140
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 4791
Size: 149 32 4610
Fragments arranged by size:
4610 149 32
PpuMI rG'GwC_Cy
Cuts at: 0 132 2839 3187 4791
Size: 132 2707 348 1604
Fragments arranged by size:
2707 1604 348 132
PstI C_TGCA'G
Cuts at: 0 252 573 647 992 3425 4791
Size: 252 321 74 345 2433 1366
Fragments arranged by size:
2433 1366 345 321 252 74
PvuII CAG'CTG
Cuts at: 0 3011 3282 4050 4773 4791
Size: 3011 271 768 723 18
Fragments arranged by size:
3011 768 723 271 18
SacI G_AGCT'C
Cuts at: 0 371 2834 4791
Size: 371 2463 1957
Fragments arranged by size:
2463 1957 371
SacII CC_GC'GG
Cuts at: 0 3382 4791
Size: 3382 1409
SanDI GG'GwC_CC
Cuts at: 0 2839 3187 4791
Size: 2839 348 1604
Fragments arranged by size:
2839 1604 348
SapI GCTCTTCn'nnn_
Cuts at: 0 554 617 1167 3153 3658 4791
Size: 554 63 550 1986 505 1133
Fragments arranged by size:
1986 1133 554 550 505 63
SexAI A'CCwGG_T
Cuts at: 0 1853 4791
Size: 1853 2938
SfcI C'TryA_G
Cuts at: 0 248 569 643 988 1920 1965 2505 3421
Size: 248 321 74 345 932 45 540 916
Cuts at: 3421 4768 4791
Size: 1347 23
Fragments arranged by size:
1347 932 916 540 345 321 248 74
45 23
SfoI GGC'GCC
Cuts at: 0 4425 4791
Size: 4425 366
SmaI CCC'GGG
Cuts at: 0 1299 3366 4791
Size: 1299 2067 1425
Fragments arranged by size:
2067 1425 1299
SmlI C'TyrA_G
Cuts at: 0 2018 2496 3916 4791
Size: 2018 478 1420 875
Fragments arranged by size:
2018 1420 875 478
SphI G_CATG'C
Cuts at: 0 2308 4791
Size: 2308 2483
SspI AAT'ATT
Cuts at: 0 4142 4791
Size: 4142 649
StuI AGG'CCT
Cuts at: 0 289 2511 3559 4791
Size: 289 2222 1048 1232
Fragments arranged by size:
2222 1232 1048 289
StyI C'CwwG_G
Cuts at: 0 104 236 1491 1534 1765 2144 2289 3434
Size: 104 132 1255 43 231 379 145 1145
Cuts at: 3434 4196 4487 4791
Size: 762 291 304
Fragments arranged by size:
1255 1145 762 379 304 291 231 145
132 104 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2218 4791
Size: 2218 2573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2094 2126 3050 3082 4791
Size: 2094 32 924 32 1709
Fragments arranged by size:
2094 1709 924 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1311 3754 3895 4791
Size: 1311 2443 141 896
Fragments arranged by size:
2443 1311 896 141
XbaI T'CTAG_A
Cuts at: 0 399 2037 4791
Size: 399 1638 2754
Fragments arranged by size:
2754 1638 399
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 143 1392 4770 4791
Size: 143 1249 3378 21
Fragments arranged by size:
3378 1249 143 21
XmaI C'CCGG_G
Cuts at: 0 1297 3364 4791
Size: 1297 2067 1427
Fragments arranged by size:
2067 1427 1297
XmnI GAAnn'nnTTC
Cuts at: 0 4469 4791
Size: 4469 322
ZraI GAC'GTC
Cuts at: 0 3681 4791
Size: 3681 1110
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AloI AlwNI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BglI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HindIII KasI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI
PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII
SanDI SapI SexAI SfcI SfoI SmaI SmlI SphI
SspI StuI StyI TaqII TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI ApaI
AscI AseI AsiSI BaeI BcgI BciVI BclI BfrBI
BglII BlpI BmtI BsaBI BsiEI BsiWI BsmI BsrGI
BssHII BstBI BstZ17I BtgZI ClaI DraI DraIII EagI
EcoRV FseI FspAI HincII HpaI KpnI MfeI NheI
NotI NruI NsiI PacI PciI PmeI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SalI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SrfI SwaI TatI
XhoI