Restriction map for
pF1KF0293
(Linear) (Six-base) MAPSORT of: FLJ00293.seq Check: 6717 from: 1 to: 3054
FLJ00293.orf
With 182 enzymes: SgfI *
March 3, 2008 11:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 295 1994 2368 2369 3054
Size: 295 1699 374 1 685
Fragments arranged by size:
1699 685 374 295 1
AatII G_ACGT'C
Cuts at: 0 1571 3054
Size: 1571 1483
AccI GT'mk_AC
Cuts at: 0 680 3054
Size: 680 2374
Acc65I G'GTAC_C
Cuts at: 0 194 3054
Size: 194 2860
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 774 818 1303 2402 3054
Size: 774 44 485 1099 652
Fragments arranged by size:
1099 774 652 485 44
AfeI AGC'GCT
Cuts at: 0 2016 3054
Size: 2016 1038
AgeI A'CCGG_T
Cuts at: 0 272 3054
Size: 272 2782
AhdI GACnn_n'nnGTC
Cuts at: 0 2517 3054
Size: 2517 537
AleI CACnn'nnGTG
Cuts at: 0 427 587 1357 1702 3054
Size: 427 160 770 345 1352
Fragments arranged by size:
1352 770 427 345 160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2391 3054
Size: 2391 663
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1759 1791 3054
Size: 1759 32 1263
Fragments arranged by size:
1759 1263 32
AlwNI CAG_nnn'CTG
Cuts at: 0 162 1086 1458 3054
Size: 162 924 372 1596
Fragments arranged by size:
1596 924 372 162
ApaI G_GGCC'C
Cuts at: 0 2391 2463 2508 2664 3054
Size: 2391 72 45 156 390
Fragments arranged by size:
2391 390 156 72 45
ApaLI G'TGCA_C
Cuts at: 0 721 3054
Size: 721 2333
AvaI C'yCGr_G
Cuts at: 0 566 953 996 1052 1130 2507 3054
Size: 566 387 43 56 78 1377 547
Fragments arranged by size:
1377 566 547 387 78 56 43
BamHI G'GATC_C
Cuts at: 0 1140 3054
Size: 1140 1914
BanI G'GyrC_C
Cuts at: 0 194 1426 2167 2963 3054
Size: 194 1232 741 796 91
Fragments arranged by size:
1232 796 741 194 91
BanII G_rGCy'C
Cuts at: 0 212 1787 1996 2121 2250 2391 2463 2508
Size: 212 1575 209 125 129 141 72 45
Cuts at: 2508 2664 2984 3005 3054
Size: 156 320 21 49
Fragments arranged by size:
1575 320 212 209 156 141 129 125
72 49 45 21
BbeI G_GCGC'C
Cuts at: 0 2967 3054
Size: 2967 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 328 3054
Size: 328 2726
BbvCI CC'TCA_GC
Cuts at: 0 386 1037 2197 3054
Size: 386 651 1160 857
Fragments arranged by size:
1160 857 651 386
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2455 2489 3054
Size: 2455 34 565
Fragments arranged by size:
2455 565 34
BglI GCCn_nnn'nGGC
Cuts at: 0 259 485 913 1683 2446 2458 3054
Size: 259 226 428 770 763 12 596
Fragments arranged by size:
770 763 596 428 259 226 12
BlpI GC'TnA_GC
Cuts at: 0 935 2258 2561 2755 3054
Size: 935 1323 303 194 299
Fragments arranged by size:
1323 935 303 299 194
Bme1580I G_kGCm'C
Cuts at: 0 725 2170 2391 2463 2508 2664 2735 3054
Size: 725 1445 221 72 45 156 71 319
Fragments arranged by size:
1445 725 319 221 156 72 71 45
BmgBI CAC'GTC
Cuts at: 0 408 2049 3054
Size: 408 1641 1005
Fragments arranged by size:
1641 1005 408
BmrI ACTGGGnnnn_n'
Cuts at: 0 2493 3054
Size: 2493 561
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 724 1630 2140 3054
Size: 724 906 510 914
Fragments arranged by size:
914 906 724 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 459 577 1903 2044 2125 2935 3054
Size: 454 5 118 1326 141 81 810 119
Fragments arranged by size:
1326 810 454 141 119 118 81 5
Bpu10I CC'TnA_GC
Cuts at: 0 91 386 1037 1945 2197 3054
Size: 91 295 651 908 252 857
Fragments arranged by size:
908 857 651 295 252 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 400 559 2758 3054
Size: 400 159 2199 296
Fragments arranged by size:
2199 400 296 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 2768 3054
Size: 2768 286
BsaAI yAC'GTr
Cuts at: 0 585 1574 3054
Size: 585 989 1480
Fragments arranged by size:
1480 989 585
BsaHI Gr'CG_yC
Cuts at: 0 1568 2964 3054
Size: 1568 1396 90
Fragments arranged by size:
1568 1396 90
BsaWI w'CCGG_w
Cuts at: 0 272 1544 2228 2651 3054
Size: 272 1272 684 423 403
Fragments arranged by size:
1272 684 423 403 272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1759 1789 3054
Size: 1759 30 1265
Fragments arranged by size:
1759 1265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 886 925 2786 3054
Size: 886 39 1861 268
Fragments arranged by size:
1861 886 268 39
BseYI C'CCAG_C
Cuts at: 0 1828 1989 3046 3054
Size: 1828 161 1057 8
Fragments arranged by size:
1828 1057 161 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 2776 2812 3054
Size: 640 2136 36 242
Fragments arranged by size:
2136 640 242 36
BsiEI CG_ry'CG
Cuts at: 0 530 2842 3054
Size: 530 2312 212
Fragments arranged by size:
2312 530 212
BsiHKAI G_wGCw'C
Cuts at: 0 212 725 968 2609 3054
Size: 212 513 243 1641 445
Fragments arranged by size:
1641 513 445 243 212
BsmBI CGTCTCn'nnnn_
Cuts at: 0 859 2931 3054
Size: 859 2072 123
Fragments arranged by size:
2072 859 123
Bsp1286I G_dGCh'C
Cuts at: 0 212 725 968 1787 1996 2121 2170 2250
Size: 212 513 243 819 209 125 49 80
Cuts at: 2250 2391 2463 2508 2609 2664 2735 2984 3005
Size: 141 72 45 101 55 71 249 21
Cuts at: 3005 3054
Size: 49
Fragments arranged by size:
819 513 249 243 212 209 141 125
101 80 72 71 55 49 49 45
21
BspEI T'CCGG_A
Cuts at: 0 1544 2228 2651 3054
Size: 1544 684 423 403
Fragments arranged by size:
1544 684 423 403
BspHI T'CATG_A
Cuts at: 0 74 3054
Size: 74 2980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 295 1994 2368 2369 3054
Size: 92 203 1699 374 1 685
Fragments arranged by size:
1699 685 374 203 92 1
BsrBI CCG'CTC
Cuts at: 0 219 971 1374 2081 2834 3054
Size: 219 752 403 707 753 220
Fragments arranged by size:
753 752 707 403 220 219
BsrDI GCAATG_nn'
Cuts at: 0 269 453 3054
Size: 269 184 2601
Fragments arranged by size:
2601 269 184
BsrFI r'CCGG_y
Cuts at: 0 272 2705 3054
Size: 272 2433 349
Fragments arranged by size:
2433 349 272
BsrGI T'GTAC_A
Cuts at: 0 1640 3054
Size: 1640 1414
BssSI C'ACGA_G
Cuts at: 0 961 2815 3054
Size: 961 1854 239
Fragments arranged by size:
1854 961 239
BstAPI GCAn_nnn'nTGC
Cuts at: 0 345 654 2037 3054
Size: 345 309 1383 1017
Fragments arranged by size:
1383 1017 345 309
BstEII G'GTnAC_C
Cuts at: 0 1704 3054
Size: 1704 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1359 3054
Size: 1359 1695
BstYI r'GATC_y
Cuts at: 0 1140 1362 2648 3054
Size: 1140 222 1286 406
Fragments arranged by size:
1286 1140 406 222
BstZ17I GTA'TAC
Cuts at: 0 681 3054
Size: 681 2373
Bsu36I CC'TnA_GG
Cuts at: 0 179 3054
Size: 179 2875
BtgI C'CryG_G
Cuts at: 0 62 1383 1523 1580 1748 2453 2799 2975
Size: 62 1321 140 57 168 705 346 176
Cuts at: 2975 3054
Size: 79
Fragments arranged by size:
1321 705 346 176 168 140 79 62
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 171 339 802 2935 3054
Size: 171 168 463 2133 119
Fragments arranged by size:
2133 463 171 168 119
BtsI GCAGTG_nn'
Cuts at: 0 636 767 908 1028 1079 2889 3054
Size: 636 131 141 120 51 1810 165
Fragments arranged by size:
1810 636 165 141 131 120 51
DraIII CAC_nnn'GTG
Cuts at: 0 2241 3054
Size: 2241 813
EaeI y'GGCC_r
Cuts at: 0 220 242 905 944 1394 1533 1675 2058
Size: 220 22 663 39 450 139 142 383
Cuts at: 2058 2320 2447 2802 2839 2882 3054
Size: 262 127 355 37 43 172
Fragments arranged by size:
663 450 383 355 262 220 172 142
139 127 43 39 37 22
EagI C'GGCC_G
Cuts at: 0 2839 3054
Size: 2839 215
EarI CTCTTCn'nnn_
Cuts at: 0 794 1292 1621 1741 2377 3054
Size: 794 498 329 120 636 677
Fragments arranged by size:
794 677 636 498 329 120
EcoICRI GAG'CTC
Cuts at: 0 210 3054
Size: 210 2844
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 454 459 577 774 818 1303 1903 2044
Size: 454 5 118 197 44 485 600 141
Cuts at: 2044 2125 2402 2935 3054
Size: 81 277 533 119
Fragments arranged by size:
600 533 485 454 277 197 141 119
118 81 44 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1285 1618 2175 3054
Size: 1285 333 557 879
Fragments arranged by size:
1285 879 557 333
EcoO109I rG'GnC_Cy
Cuts at: 0 16 522 570 894 957 2459 2460 2661
Size: 16 506 48 324 63 1502 1 201
Cuts at: 2661 3054
Size: 393
Fragments arranged by size:
1502 506 393 324 201 63 48 16
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1803 3054
Size: 1803 1251
HaeII r_GCGC'y
Cuts at: 0 1716 2018 2187 2967 3054
Size: 1716 302 169 780 87
Fragments arranged by size:
1716 780 302 169 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 524 556 793 825 1928 1960 2503 2535
Size: 524 32 237 32 1103 32 543 32
Cuts at: 2535 3054
Size: 519
Fragments arranged by size:
1103 543 524 519 237 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1212 3054
Size: 1212 1842
KasI G'GCGC_C
Cuts at: 0 2963 3054
Size: 2963 91
KpnI G_GTAC'C
Cuts at: 0 198 3054
Size: 198 2856
MfeI C'AATT_G
Cuts at: 0 616 3054
Size: 616 2438
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2021 2954 3054
Size: 2021 933 100
Fragments arranged by size:
2021 933 100
MscI TGG'CCA
Cuts at: 0 244 2060 3054
Size: 244 1816 994
Fragments arranged by size:
1816 994 244
MslI CAynn'nnrTG
Cuts at: 0 233 427 587 718 1174 1357 1702 2137
Size: 233 194 160 131 456 183 345 435
Cuts at: 2137 2215 2974 3054
Size: 78 759 80
Fragments arranged by size:
759 456 435 345 233 194 183 160
131 80 78
MspA1I CmG'CkG
Cuts at: 0 645 1036 1083 1799 2268 2325 2397 2768
Size: 645 391 47 716 469 57 72 371
Cuts at: 2768 2781 2801 3014 3054
Size: 13 20 213 40
Fragments arranged by size:
716 645 469 391 371 213 72 57
47 40 20 13
NarI GG'CG_CC
Cuts at: 0 2964 3054
Size: 2964 90
NcoI C'CATG_G
Cuts at: 0 62 1523 1748 2453 3054
Size: 62 1461 225 705 601
Fragments arranged by size:
1461 705 601 225 62
NspI r_CATG'y
Cuts at: 0 2427 3054
Size: 2427 627
PflMI CCAn_nnn'nTGG
Cuts at: 0 1989 2784 3054
Size: 1989 795 270
Fragments arranged by size:
1989 795 270
PfoI T'CCnGG_A
Cuts at: 0 1619 2021 2351 3054
Size: 1619 402 330 703
Fragments arranged by size:
1619 703 402 330
PmlI CAC'GTG
Cuts at: 0 585 3054
Size: 585 2469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1759 1791 3054
Size: 1759 32 1263
Fragments arranged by size:
1759 1263 32
PpuMI rG'GwC_Cy
Cuts at: 0 522 570 957 3054
Size: 522 48 387 2097
Fragments arranged by size:
2097 522 387 48
PshAI GACnn'nnGTC
Cuts at: 0 2095 3054
Size: 2095 959
PspOMI G'GGCC_C
Cuts at: 0 2387 2459 2504 2660 3054
Size: 2387 72 45 156 394
Fragments arranged by size:
2387 394 156 72 45
PstI C_TGCA'G
Cuts at: 0 89 887 1035 1259 1417 1454 2042 2267
Size: 89 798 148 224 158 37 588 225
Cuts at: 2267 2345 2380 3054
Size: 78 35 674
Fragments arranged by size:
798 674 588 225 224 158 148 89
78 37 35
PvuI CG_AT'CG
Cuts at: 0 530 3054
Size: 530 2524
PvuII CAG'CTG
Cuts at: 0 645 1036 2397 2781 3054
Size: 645 391 1361 384 273
Fragments arranged by size:
1361 645 391 384 273
SacI G_AGCT'C
Cuts at: 0 212 3054
Size: 212 2842
SacII CC_GC'GG
Cuts at: 0 2802 3054
Size: 2802 252
SanDI GG'GwC_CC
Cuts at: 0 522 3054
Size: 522 2532
SexAI A'CCwGG_T
Cuts at: 0 1859 3054
Size: 1859 1195
SfcI C'TryA_G
Cuts at: 0 85 883 1031 1255 1413 1450 2038 2263
Size: 85 798 148 224 158 37 588 225
Cuts at: 2263 2341 2376 3054
Size: 78 35 678
Fragments arranged by size:
798 678 588 225 224 158 148 85
78 37 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2446 3054
Size: 2446 608
SfoI GGC'GCC
Cuts at: 0 2965 3054
Size: 2965 89
SmaI CCC'GGG
Cuts at: 0 955 1054 1132 3054
Size: 955 99 78 1922
Fragments arranged by size:
1922 955 99 78
SmlI C'TyrA_G
Cuts at: 0 415 574 2737 3054
Size: 415 159 2163 317
Fragments arranged by size:
2163 415 317 159
StuI AGG'CCT
Cuts at: 0 439 1411 3054
Size: 439 972 1643
Fragments arranged by size:
1643 972 439
StyI C'CwwG_G
Cuts at: 0 62 731 1067 1523 1748 1778 2171 2441
Size: 62 669 336 456 225 30 393 270
Cuts at: 2441 2453 3054
Size: 12 601
Fragments arranged by size:
669 601 456 393 336 270 225 62
30 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 577 781 1086 1454 3054
Size: 577 204 305 368 1600
Fragments arranged by size:
1600 577 368 305 204
TatI w'GTAC_w
Cuts at: 0 350 491 1640 3054
Size: 350 141 1149 1414
Fragments arranged by size:
1414 1149 350 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1101 1133 2520 2552 3054
Size: 1101 32 1387 32 502
Fragments arranged by size:
1387 1101 502 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1165 3054
Size: 1165 1889
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 429 840 1362 1755 1986 3054
Size: 429 411 522 393 231 1068
Fragments arranged by size:
1068 522 429 411 393 231
XmaI C'CCGG_G
Cuts at: 0 953 1052 1130 3054
Size: 953 99 78 1924
Fragments arranged by size:
1924 953 99 78
XmnI GAAnn'nnTTC
Cuts at: 0 2638 3054
Size: 2638 416
ZraI GAC'GTC
Cuts at: 0 1569 3054
Size: 1569 1485
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
KasI KpnI MfeI MmeI MscI MslI MspA1I NarI
NcoI NspI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII SacI SacII SanDI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII ApoI AscI AseI AsiSI AvrII
BaeI BciVI BclI BfrBI BglII BmtI BsaBI BsiWI
BsmI BssHII BstBI ClaI CspCI DraI DrdI EciI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PsiI PspXI PsrI RsrII SalI
SapI SbfI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI