Restriction map for
pF1KF0316
(Linear) (Six-base) MAPSORT of: FLJ00316.seq Check: 9410 from: 1 to: 1920
FLJ00316.orf
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 1920
Size: 1723 197
AflII C'TTAA_G
Cuts at: 0 1273 1723 1920
Size: 1273 450 197
Fragments arranged by size:
1273 450 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 528 1920
Size: 528 1392
AlwNI CAG_nnn'CTG
Cuts at: 0 52 459 1021 1068 1098 1554 1920
Size: 52 407 562 47 30 456 366
Fragments arranged by size:
562 456 407 366 52 47 30
ApoI r'AATT_y
Cuts at: 0 114 683 690 1177 1316 1732 1920
Size: 114 569 7 487 139 416 188
Fragments arranged by size:
569 487 416 188 139 114 7
AseI AT'TA_AT
Cuts at: 0 1769 1920
Size: 1769 151
BanII G_rGCy'C
Cuts at: 0 250 1920
Size: 250 1670
BbsI GAAGACnn'nnnn_
Cuts at: 0 1134 1920
Size: 1134 786
BbvCI CC'TCA_GC
Cuts at: 0 533 1920
Size: 533 1387
BlpI GC'TnA_GC
Cuts at: 0 1268 1920
Size: 1268 652
Bme1580I G_kGCm'C
Cuts at: 0 32 296 1394 1920
Size: 32 264 1098 526
Fragments arranged by size:
1098 526 264 32
BmgBI CAC'GTC
Cuts at: 0 1107 1920
Size: 1107 813
BmtI G_CTAG'C
Cuts at: 0 490 1844 1920
Size: 490 1354 76
Fragments arranged by size:
1354 490 76
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 525 619 664 997 1075 1352 1408
Size: 481 44 94 45 333 78 277 56
Cuts at: 1408 1514 1920
Size: 106 406
Fragments arranged by size:
481 406 333 277 106 94 78 56
45 44
Bpu10I CC'TnA_GC
Cuts at: 0 533 1920
Size: 533 1387
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1583 1618 1920
Size: 1027 556 35 302
Fragments arranged by size:
1027 556 302 35
BsaAI yAC'GTr
Cuts at: 0 1915 1920
Size: 1915 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 592 622 1058 1088 1211 1241 1920
Size: 592 30 436 30 123 30 679
Fragments arranged by size:
679 592 436 123 30 30 30
BseYI C'CCAG_C
Cuts at: 0 918 1001 1014 1061 1920
Size: 918 83 13 47 859
Fragments arranged by size:
918 859 83 47 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 454 1045 1920
Size: 277 177 591 875
Fragments arranged by size:
875 591 277 177
BsiHKAI G_wGCw'C
Cuts at: 0 442 1154 1292 1920
Size: 442 712 138 628
Fragments arranged by size:
712 628 442 138
BsmI GAATG_Cn'
Cuts at: 0 654 987 1920
Size: 654 333 933
Fragments arranged by size:
933 654 333
Bsp1286I G_dGCh'C
Cuts at: 0 32 250 296 442 1154 1292 1394 1920
Size: 32 218 46 146 712 138 102 526
Fragments arranged by size:
712 526 218 146 138 102 46 32
BspHI T'CATG_A
Cuts at: 0 62 206 1920
Size: 62 144 1714
Fragments arranged by size:
1714 144 62
BsrBI CCG'CTC
Cuts at: 0 1518 1920
Size: 1518 402
BsrDI GCAATG_nn'
Cuts at: 0 237 1133 1920
Size: 237 896 787
Fragments arranged by size:
896 787 237
BsrFI r'CCGG_y
Cuts at: 0 755 1920
Size: 755 1165
BssSI C'ACGA_G
Cuts at: 0 381 1920
Size: 381 1539
BstXI CCAn_nnnn'nTGG
Cuts at: 0 66 1920
Size: 66 1854
BstYI r'GATC_y
Cuts at: 0 386 1826 1920
Size: 386 1440 94
Fragments arranged by size:
1440 386 94
BtgI C'CryG_G
Cuts at: 0 155 251 1920
Size: 155 96 1669
Fragments arranged by size:
1669 155 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 365 1920
Size: 365 1555
BtsI GCAGTG_nn'
Cuts at: 0 32 992 1487 1920
Size: 32 960 495 433
Fragments arranged by size:
960 495 433 32
DraI TTT'AAA
Cuts at: 0 782 1920
Size: 782 1138
DraIII CAC_nnn'GTG
Cuts at: 0 477 1920
Size: 477 1443
EaeI y'GGCC_r
Cuts at: 0 167 1920
Size: 167 1753
EarI CTCTTCn'nnn_
Cuts at: 0 20 92 1235 1920
Size: 20 72 1143 685
Fragments arranged by size:
1143 685 72 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 525 619 664 997 1075 1352 1408
Size: 481 44 94 45 333 78 277 56
Cuts at: 1408 1514 1723 1920
Size: 106 209 197
Fragments arranged by size:
481 333 277 209 197 106 94 78
56 45 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 889 1122 1920
Size: 889 233 798
Fragments arranged by size:
889 798 233
EcoO109I rG'GnC_Cy
Cuts at: 0 342 456 1419 1920
Size: 342 114 963 501
Fragments arranged by size:
963 501 342 114
EcoRI G'AATT_C
Cuts at: 0 114 1177 1920
Size: 114 1063 743
Fragments arranged by size:
1063 743 114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1239 1738 1920
Size: 1239 499 182
Fragments arranged by size:
1239 499 182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 698 730 881 913 1328 1360 1581 1613
Size: 698 32 151 32 415 32 221 32
Cuts at: 1613 1845 1877 1887 1919 1920
Size: 232 32 10 32 1
Fragments arranged by size:
698 415 232 221 151 32 32 32
32 32 32 10 1
HindIII A'AGCT_T
Cuts at: 0 1189 1608 1920
Size: 1189 419 312
Fragments arranged by size:
1189 419 312
MscI TGG'CCA
Cuts at: 0 169 1920
Size: 169 1751
MslI CAynn'nnrTG
Cuts at: 0 64 280 1920
Size: 64 216 1640
Fragments arranged by size:
1640 216 64
MspA1I CmG'CkG
Cuts at: 0 105 468 1018 1513 1537 1920
Size: 105 363 550 495 24 383
Fragments arranged by size:
550 495 383 363 105 24
NdeI CA'TA_TG
Cuts at: 0 788 1920
Size: 788 1132
NheI G'CTAG_C
Cuts at: 0 486 1840 1920
Size: 486 1354 80
Fragments arranged by size:
1354 486 80
NspI r_CATG'y
Cuts at: 0 702 1114 1920
Size: 702 412 806
Fragments arranged by size:
806 702 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 459 1056 1920
Size: 321 138 597 864
Fragments arranged by size:
864 597 321 138
PfoI T'CCnGG_A
Cuts at: 0 1091 1329 1613 1920
Size: 1091 238 284 307
Fragments arranged by size:
1091 307 284 238
PpuMI rG'GwC_Cy
Cuts at: 0 1419 1920
Size: 1419 501
PstI C_TGCA'G
Cuts at: 0 27 1235 1439 1472 1920
Size: 27 1208 204 33 448
Fragments arranged by size:
1208 448 204 33 27
PvuII CAG'CTG
Cuts at: 0 105 468 1537 1920
Size: 105 363 1069 383
Fragments arranged by size:
1069 383 363 105
SapI GCTCTTCn'nnn_
Cuts at: 0 92 1920
Size: 92 1828
SexAI A'CCwGG_T
Cuts at: 0 36 1920
Size: 36 1884
SfcI C'TryA_G
Cuts at: 0 23 1044 1231 1435 1468 1920
Size: 23 1021 187 204 33 452
Fragments arranged by size:
1021 452 204 187 33 23
SmlI C'TyrA_G
Cuts at: 0 1006 1273 1598 1633 1723 1920
Size: 1006 267 325 35 90 197
Fragments arranged by size:
1006 325 267 197 90 35
SphI G_CATG'C
Cuts at: 0 1114 1920
Size: 1114 806
SspI AAT'ATT
Cuts at: 0 778 1258 1920
Size: 778 480 662
Fragments arranged by size:
778 662 480
SwaI ATTT'AAAT
Cuts at: 0 782 1920
Size: 782 1138
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 1920
Size: 880 1040
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 273 305 1211 1243 1920
Size: 273 32 906 32 677
Fragments arranged by size:
906 677 273 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 572 1920
Size: 81 491 1348
Fragments arranged by size:
1348 491 81
XmnI GAAnn'nnTTC
Cuts at: 0 1664 1733 1920
Size: 1664 69 187
Fragments arranged by size:
1664 187 69
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApoI AseI BanII BbsI
BbvCI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BpuEI
BsaAI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrBI BsrDI BsrFI BssSI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HindIII MscI MslI MspA1I NdeI
NheI NspI PflMI PfoI PpuMI PstI PvuII SapI
SexAI SfcI SmlI SphI SspI SwaI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BglII BmrI BplI BsaI BsaBI BsaHI
BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspMI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NgoMIV NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI StyI TatI Tth111I XbaI XhoI
XmaI ZraI