Restriction map for
pF1KF0343
(Linear) (Six-base) MAPSORT of: FLJ00343.seq Check: 2228 from: 1 to: 7917
FLJ00343.orf
With 182 enzymes: SgfI *
September 25, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3732 7917
Size: 3732 4185
AatII G_ACGT'C
Cuts at: 0 2411 2726 7187 7917
Size: 2411 315 4461 730
Fragments arranged by size:
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AccI GT'mk_AC
Cuts at: 0 62 2321 4499 4810 7580 7715 7917
Size: 62 2259 2178 311 2770 135 202
Fragments arranged by size:
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Acc65I G'GTAC_C
Cuts at: 0 4587 7917
Size: 4587 3330
AclI AA'CG_TT
Cuts at: 0 6440 7917
Size: 6440 1477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 421 546 826 1564 2259 5992 6413
Size: 190 231 125 280 738 695 3733 421
Cuts at: 6413 6749 7917
Size: 336 1168
Fragments arranged by size:
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190 125
AflIII A'CryG_T
Cuts at: 0 317 3361 7917
Size: 317 3044 4556
Fragments arranged by size:
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AleI CACnn'nnGTG
Cuts at: 0 4675 7858 7917
Size: 4675 3183 59
Fragments arranged by size:
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AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4033 7756 7917
Size: 4033 3723 161
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2713 2745 5786 5818 7917
Size: 2713 32 3041 32 2099
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 1231 4948 5244 6758 7917
Size: 1231 3717 296 1514 1159
Fragments arranged by size:
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ApaI G_GGCC'C
Cuts at: 0 591 1564 1750 2638 3031 3119 3319 4183
Size: 591 973 186 888 393 88 200 864
Cuts at: 4183 4367 4469 4553 5292 5689 6235 6671 6712
Size: 184 102 84 739 397 546 436 41
Cuts at: 6712 6808 7623 7917
Size: 96 815 294
Fragments arranged by size:
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88 84 41
ApaLI G'TGCA_C
Cuts at: 0 1366 4932 5031 5040 7078 7917
Size: 1366 3566 99 9 2038 839
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 6748 7917
Size: 6748 1169
AvaI C'yCGr_G
Cuts at: 0 17 331 618 730 1042 1307 1437 2519
Size: 17 314 287 112 312 265 130 1082
Cuts at: 2519 3033 3334 3391 3502 3555 3676 3845 4067
Size: 514 301 57 111 53 121 169 222
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Size: 590 1168 121 818 393 466 294
Fragments arranged by size:
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AvrII C'CTAG_G
Cuts at: 0 6672 7917
Size: 6672 1245
BamHI G'GATC_C
Cuts at: 0 524 2859 4755 7917
Size: 524 2335 1896 3162
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 595 1711 3045 3280 4038 4102 4258 4513
Size: 595 1116 1334 235 758 64 156 255
Cuts at: 4513 4587 5452 6942 7198 7354 7446 7911 7917
Size: 74 865 1490 256 156 92 465 6
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BanII G_rGCy'C
Cuts at: 0 591 819 878 1105 1178 1564 1750 2351
Size: 591 228 59 227 73 386 186 601
Cuts at: 2351 2366 2603 2638 2878 3031 3119 3250 3319
Size: 15 237 35 240 153 88 131 69
Cuts at: 3319 3392 3852 4183 4367 4378 4469 4553 4600
Size: 73 460 331 184 11 91 84 47
Cuts at: 4600 4664 5292 5689 5963 6215 6235 6426 6649
Size: 64 628 397 274 252 20 191 223
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Size: 22 19 22 96 290 525 294
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35 22 22 20 19 15 11
BbeI G_GCGC'C
Cuts at: 0 599 3284 7917
Size: 599 2685 4633
Fragments arranged by size:
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BbsI GAAGACnn'nnnn_
Cuts at: 0 1961 2056 3801 4783 7917
Size: 1961 95 1745 982 3134
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 1295 1966 2911 6460 7917
Size: 1295 671 945 3549 1457
Fragments arranged by size:
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BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 171 205 366 400 1677 1711 2640 2674
Size: 171 34 161 34 1277 34 929 34
Cuts at: 2674 7917
Size: 5243
Fragments arranged by size:
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34
BclI T'GATC_A
Cuts at: 0 722 4988 6089 7917
Size: 722 4266 1101 1828
Fragments arranged by size:
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BglI GCCn_nnn'nGGC
Cuts at: 0 1450 3889 6521 7060 7418 7917
Size: 1450 2439 2632 539 358 499
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 1214 3587 6821 7917
Size: 1214 2373 3234 1096
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 250 6214 7675 7917
Size: 250 5964 1461 242
Fragments arranged by size:
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Bme1580I G_kGCm'C
Cuts at: 0 591 618 1285 1370 1391 1564 1714 1750
Size: 591 27 667 85 21 173 150 36
Cuts at: 1750 1822 2210 2433 2638 3031 3050 3119 3152
Size: 72 388 223 205 393 19 69 33
Cuts at: 3152 3319 3383 3722 4022 4043 4183 4367 4469
Size: 167 64 339 300 21 140 184 102
Cuts at: 4469 4538 4553 4913 4936 5035 5044 5171 5292
Size: 69 15 360 23 99 9 127 121
Cuts at: 5292 5689 6014 6235 6601 6671 6712 6808 7082
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BmgBI CAC'GTC
Cuts at: 0 1371 1815 3876 3996 4494 4833 5030 5073
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Cuts at: 5073 5802 6278 7917
Size: 729 476 1639
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 645 780 1172 7917
Size: 645 135 392 6745
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3609 4200 7917
Size: 3609 591 3717
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 1321 1453 1777 2456 3529 6286 6739
Size: 1192 129 132 324 679 1073 2757 453
Cuts at: 6739 6755 6800 7120 7917
Size: 16 45 320 797
Fragments arranged by size:
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Bpu10I CC'TnA_GC
Cuts at: 0 211 1295 1966 2911 3495 4372 6460 7917
Size: 211 1084 671 945 584 877 2088 1457
Fragments arranged by size:
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BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 2335 7819 7917
Size: 331 2004 5484 98
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 913 3910 4129 5240 6135 7917
Size: 913 2997 219 1111 895 1782
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 957 1119 1664 1938 2032 2205 3378 3459
Size: 957 162 545 274 94 173 1173 81
Cuts at: 3459 4808 4824 5166 5994 6063 6348 6636 7689
Size: 1349 16 342 828 69 285 288 1053
Cuts at: 7689 7800 7917
Size: 111 117
Fragments arranged by size:
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285 274 173 162 117 111 94 81
69 16
BsaBI GATnn'nnATC
Cuts at: 0 5503 7917
Size: 5503 2414
BsaHI Gr'CG_yC
Cuts at: 0 59 74 596 650 1139 1355 1766 2252
Size: 59 15 522 54 489 216 411 486
Cuts at: 2252 2408 2612 2723 2837 3281 3842 4721 4922
Size: 156 204 111 114 444 561 879 201
Cuts at: 4922 6417 6968 7184 7643 7917
Size: 1495 551 216 459 274
Fragments arranged by size:
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114 111 59 54 15
BsaWI w'CCGG_w
Cuts at: 0 3766 3862 7917
Size: 3766 96 4055
Fragments arranged by size:
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BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 2182 2212 2930 2960 7366 7396
Size: 433 30 1719 30 718 30 4406 30
Cuts at: 7396 7450 7480 7917
Size: 54 30 437
Fragments arranged by size:
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BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 487 496 748 997 1588 3988 4993
Size: 484 3 9 252 249 591 2400 1005
Cuts at: 4993 5971 7117 7795 7917
Size: 978 1146 678 122
Fragments arranged by size:
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BseYI C'CCAG_C
Cuts at: 0 705 1824 3523 3730 4021 4537 5120 5200
Size: 705 1119 1699 207 291 516 583 80
Cuts at: 5200 5476 6235 6703 6808 7314 7610 7917
Size: 276 759 468 105 506 296 307
Fragments arranged by size:
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BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 3059 3967 5239 5476 5694 6300 7917
Size: 421 2638 908 1272 237 218 606 1617
Fragments arranged by size:
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BsiEI CG_ry'CG
Cuts at: 0 585 971 3614 4235 7067 7917
Size: 585 386 2643 621 2832 850
Fragments arranged by size:
2832 2643 850 621 585 386
BsiHKAI G_wGCw'C
Cuts at: 0 167 251 764 1076 1370 3496 4360 4936
Size: 167 84 513 312 294 2126 864 576
Cuts at: 4936 5035 5044 5165 6032 7082 7883 7917
Size: 99 9 121 867 1050 801 34
Fragments arranged by size:
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BsmI GAATG_Cn'
Cuts at: 0 5774 7917
Size: 5774 2143
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4975 5899 6013 7540 7917
Size: 4975 924 114 1527 377
Fragments arranged by size:
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Bsp1286I G_dGCh'C
Cuts at: 0 167 251 591 618 764 819 878 1076
Size: 167 84 340 27 146 55 59 198
Cuts at: 1076 1105 1178 1285 1370 1391 1564 1714 1750
Size: 29 73 107 85 21 173 150 36
Cuts at: 1750 1822 2210 2351 2366 2433 2603 2638 2878
Size: 72 388 141 15 67 170 35 240
Cuts at: 2878 3031 3050 3119 3152 3250 3319 3383 3392
Size: 153 19 69 33 98 69 64 9
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Size: 104 226 130 170 21 140 177 7
Cuts at: 4367 4378 4469 4538 4553 4600 4664 4913 4936
Size: 11 91 69 15 47 64 249 23
Cuts at: 4936 5035 5044 5165 5171 5292 5689 5963 6014
Size: 99 9 121 6 121 397 274 51
Cuts at: 6014 6032 6215 6235 6426 6601 6649 6671 6690
Size: 18 183 20 191 175 48 22 19
Cuts at: 6690 6712 6808 7082 7098 7359 7414 7608 7623
Size: 22 96 274 16 261 55 194 15
Cuts at: 7623 7883 7916 7917
Size: 260 33 1
Fragments arranged by size:
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240 226 198 194 191 183 177 175
173 170 170 167 153 150 146 141
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69 67 64 64 59 55 55 51
48 47 36 35 33 33 29 27
23 22 22 21 21 20 19 19
18 16 15 15 15 11 9 9
7 6 1
BspHI T'CATG_A
Cuts at: 0 770 2607 4626 7917
Size: 770 1837 2019 3291
Fragments arranged by size:
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BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 206 3732 3759 4440 5756 7276 7838 7917
Size: 206 3526 27 681 1316 1520 562 79
Fragments arranged by size:
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BsrBI CCG'CTC
Cuts at: 0 581 3513 7917
Size: 581 2932 4404
Fragments arranged by size:
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BsrDI GCAATG_nn'
Cuts at: 0 2187 2490 4444 6791 7347 7917
Size: 2187 303 1954 2347 556 570
Fragments arranged by size:
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BsrFI r'CCGG_y
Cuts at: 0 84 1381 2217 3283 7808 7917
Size: 84 1297 836 1066 4525 109
Fragments arranged by size:
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BsrGI T'GTAC_A
Cuts at: 0 1514 3620 3947 7917
Size: 1514 2106 327 3970
Fragments arranged by size:
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BssSI C'ACGA_G
Cuts at: 0 2359 5494 7335 7558 7917
Size: 2359 3135 1841 223 359
Fragments arranged by size:
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BstAPI GCAn_nnn'nTGC
Cuts at: 0 5039 6036 7917
Size: 5039 997 1881
Fragments arranged by size:
5039 1881 997
BstBI TT'CG_AA
Cuts at: 0 1697 7917
Size: 1697 6220
BstEII G'GTnAC_C
Cuts at: 0 1548 3132 6285 6507 7365 7917
Size: 1548 1584 3153 222 858 552
Fragments arranged by size:
3153 1584 1548 858 552 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3024 3436 3640 3781 4476 6084 6243 7210
Size: 3024 412 204 141 695 1608 159 967
Cuts at: 7210 7653 7668 7917
Size: 443 15 249
Fragments arranged by size:
3024 1608 967 695 443 412 249 204
159 141 15
BstYI r'GATC_y
Cuts at: 0 128 524 1214 2859 3587 4755 4877 5910
Size: 128 396 690 1645 728 1168 122 1033
Cuts at: 5910 6821 7226 7784 7917
Size: 911 405 558 133
Fragments arranged by size:
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BstZ17I GTA'TAC
Cuts at: 0 2322 4811 7917
Size: 2322 2489 3106
Fragments arranged by size:
3106 2489 2322
Bsu36I CC'TnA_GG
Cuts at: 0 3900 5338 5409 7089 7415 7917
Size: 3900 1438 71 1680 326 502
Fragments arranged by size:
3900 1680 1438 502 326 71
BtgI C'CryG_G
Cuts at: 0 118 1268 1346 1631 2234 3633 3933 3989
Size: 118 1150 78 285 603 1399 300 56
Cuts at: 3989 4080 4694 5604 6662 7442 7917
Size: 91 614 910 1058 780 475
Fragments arranged by size:
1399 1150 1058 910 780 614 603 475
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BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 174 1077 1173 1832 3953 6209 7917
Size: 174 903 96 659 2121 2256 1708
Fragments arranged by size:
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BtsI GCAGTG_nn'
Cuts at: 0 1539 2762 3246 5579 5819 7917
Size: 1539 1223 484 2333 240 2098
Fragments arranged by size:
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ClaI AT'CG_AT
Cuts at: 0 4709 7067 7917
Size: 4709 2358 850
Fragments arranged by size:
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CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1191 1226 2085 2120 4848 4883 7917
Size: 1191 35 859 35 2728 35 3034
Fragments arranged by size:
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DraIII CAC_nnn'GTG
Cuts at: 0 1135 1365 1718 3990 5039 6036 7917
Size: 1135 230 353 2272 1049 997 1881
Fragments arranged by size:
2272 1881 1135 1049 997 353 230
DrdI GACnn_nn'nnGTC
Cuts at: 0 780 4341 6892 7917
Size: 780 3561 2551 1025
Fragments arranged by size:
3561 2551 1025 780
EaeI y'GGCC_r
Cuts at: 0 86 582 933 968 1083 1422 1458 1906
Size: 86 496 351 35 115 339 36 448
Cuts at: 1906 2060 2397 3611 3708 3890 4287 5274 5571
Size: 154 337 1214 97 182 397 987 297
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Size: 259
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115 104 97 86 66 61 36 35
11
EagI C'GGCC_G
Cuts at: 0 582 968 3611 7917
Size: 582 386 2643 4306
Fragments arranged by size:
4306 2643 582 386
EarI CTCTTCn'nnn_
Cuts at: 0 3421 4577 7693 7917
Size: 3421 1156 3116 224
Fragments arranged by size:
3421 3116 1156 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 286 709 1951 7917
Size: 121 165 423 1242 5966
Fragments arranged by size:
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Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 421 546 826 1192 1321 1453 1564
Size: 190 231 125 280 366 129 132 111
Cuts at: 1564 1777 2259 2456 3529 5992 6286 6413 6739
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Cuts at: 6739 6749 6755 6800 7120 7917
Size: 10 6 45 320 797
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280 231 213 197 190 132 129 127
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EcoNI CCTnn'n_nnAGG
Cuts at: 0 106 7834 7917
Size: 106 7728 83
Fragments arranged by size:
7728 106 83
EcoO109I rG'GnC_Cy
Cuts at: 0 99 588 963 1162 1263 1464 1560 1561
Size: 99 489 375 199 101 201 96 1
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Size: 32 153 1 365 334 188 1 393
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Size: 137 148 84 1 181 558 11 42
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Size: 322 21 456 90 1 29 406 1
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42 40 38 32 29 21 11 7
1 1 1 1 1 1 1 1
1 1 1 1
EcoRI G'AATT_C
Cuts at: 0 6748 7917
Size: 6748 1169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1122 7722 7917
Size: 1122 6600 195
Fragments arranged by size:
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HaeII r_GCGC'y
Cuts at: 0 331 599 1529 3284 7917
Size: 331 268 930 1755 4633
Fragments arranged by size:
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Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 625 657 748 780 1041 1073
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Cuts at: 7398 7552 7584 7917
Size: 154 32 333
Fragments arranged by size:
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154 91 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 63 2067 5565 5640 7017 7917
Size: 63 2004 3498 75 1377 900
Fragments arranged by size:
3498 2004 1377 900 75 63
KasI G'GCGC_C
Cuts at: 0 595 3280 7917
Size: 595 2685 4637
Fragments arranged by size:
4637 2685 595
KpnI G_GTAC'C
Cuts at: 0 4591 7917
Size: 4591 3326
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 6080 7917
Size: 221 5859 1837
Fragments arranged by size:
5859 1837 221
MscI TGG'CCA
Cuts at: 0 935 1085 1424 2399 3892 4289 5573 5677
Size: 935 150 339 975 1493 397 1284 104
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MslI CAynn'nnrTG
Cuts at: 0 451 457 2233 2542 3638 4675 5143 5851
Size: 451 6 1776 309 1096 1037 468 708
Cuts at: 5851 6082 7858 7917
Size: 231 1776 59
Fragments arranged by size:
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231 59 6
MspA1I CmG'CkG
Cuts at: 0 285 546 611 1299 1328 1501 2195 3043
Size: 285 261 65 688 29 173 694 848
Cuts at: 3043 3523 4226 5244 5708 6589 7318 7917
Size: 480 703 1018 464 881 729 599
Fragments arranged by size:
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NaeI GCC'GGC
Cuts at: 0 86 1383 3285 7810 7917
Size: 86 1297 1902 4525 107
Fragments arranged by size:
4525 1902 1297 107 86
NarI GG'CG_CC
Cuts at: 0 596 3281 7917
Size: 596 2685 4636
Fragments arranged by size:
4636 2685 596
NcoI C'CATG_G
Cuts at: 0 1268 1346 1631 2234 5604 7442 7917
Size: 1268 78 285 603 3370 1838 475
Fragments arranged by size:
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NgoMIV G'CCGG_C
Cuts at: 0 84 1381 3283 7808 7917
Size: 84 1297 1902 4525 109
Fragments arranged by size:
4525 1902 1297 109 84
NspI r_CATG'y
Cuts at: 0 3365 3948 5523 5735 6043 7743 7917
Size: 3365 583 1575 212 308 1700 174
Fragments arranged by size:
3365 1700 1575 583 308 212 174
PasI CC'CwG_GG
Cuts at: 0 591 1750 5268 7917
Size: 591 1159 3518 2649
Fragments arranged by size:
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PciI A'CATG_T
Cuts at: 0 3361 7917
Size: 3361 4556
PflMI CCAn_nnn'nTGG
Cuts at: 0 1664 6063 7917
Size: 1664 4399 1854
Fragments arranged by size:
4399 1854 1664
PfoI T'CCnGG_A
Cuts at: 0 338 1259 2132 3626 6488 6872 7917
Size: 338 921 873 1494 2862 384 1045
Fragments arranged by size:
2862 1494 1045 921 873 384 338
PmlI CAC'GTG
Cuts at: 0 1664 1938 2032 3459 5166 5994 6063 6348
Size: 1664 274 94 1427 1707 828 69 285
Cuts at: 6348 6636 7800 7917
Size: 288 1164 117
Fragments arranged by size:
1707 1664 1427 1164 828 288 285 274
117 94 69
PpuMI rG'GwC_Cy
Cuts at: 0 99 963 1162 1263 1593 4317 6261 7257
Size: 99 864 199 101 330 2724 1944 996
Cuts at: 7257 7627 7769 7917
Size: 370 142 148
Fragments arranged by size:
2724 1944 996 864 370 330 199 148
142 101 99
PshAI GACnn'nnGTC
Cuts at: 0 4010 7917
Size: 4010 3907
PspOMI G'GGCC_C
Cuts at: 0 587 1560 1746 2634 3027 3115 3315 4179
Size: 587 973 186 888 393 88 200 864
Cuts at: 4179 4363 4465 4549 5288 5685 6231 6667 6708
Size: 184 102 84 739 397 546 436 41
Cuts at: 6708 6804 7619 7917
Size: 96 815 298
Fragments arranged by size:
973 888 864 815 739 587 546 436
397 393 298 200 186 184 102 96
88 84 41
PspXI vC'TCGA_Gb
Cuts at: 0 331 3334 3555 7917
Size: 331 3003 221 4362
Fragments arranged by size:
4362 3003 331 221
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6550 6582 7917
Size: 6550 32 1335
Fragments arranged by size:
6550 1335 32
PstI C_TGCA'G
Cuts at: 0 203 1333 3233 3743 6174 7723 7917
Size: 203 1130 1900 510 2431 1549 194
Fragments arranged by size:
2431 1900 1549 1130 510 203 194
PvuI CG_AT'CG
Cuts at: 0 7067 7917
Size: 7067 850
PvuII CAG'CTG
Cuts at: 0 546 611 1299 1501 2195 4226 5244 5708
Size: 546 65 688 202 694 2031 1018 464
Cuts at: 5708 7318 7917
Size: 1610 599
Fragments arranged by size:
2031 1610 1018 694 688 599 546 464
202 65
SalI G'TCGA_C
Cuts at: 0 61 7917
Size: 61 7856
SanDI GG'GwC_CC
Cuts at: 0 7257 7917
Size: 7257 660
SapI GCTCTTCn'nnn_
Cuts at: 0 7693 7917
Size: 7693 224
ScaI AGT'ACT
Cuts at: 0 4568 7917
Size: 4568 3349
SexAI A'CCwGG_T
Cuts at: 0 6032 7917
Size: 6032 1885
SfcI C'TryA_G
Cuts at: 0 199 1329 3229 3739 4172 4263 4554 5769
Size: 199 1130 1900 510 433 91 291 1215
Cuts at: 5769 6170 7719 7815 7917
Size: 401 1549 96 102
Fragments arranged by size:
1900 1549 1215 1130 510 433 401 291
199 102 96 91
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3889 7917
Size: 3889 4028
SfoI GGC'GCC
Cuts at: 0 597 3282 7917
Size: 597 2685 4635
Fragments arranged by size:
4635 2685 597
SmaI CCC'GGG
Cuts at: 0 620 1309 2521 3393 3504 3678 3847 4069
Size: 620 689 1212 872 111 174 169 222
Cuts at: 4069 4659 6766 7625 7917
Size: 590 2107 859 292
Fragments arranged by size:
2107 1212 872 859 689 620 590 292
222 174 169 111
SmlI C'TyrA_G
Cuts at: 0 310 331 2350 3334 3555 7834 7917
Size: 310 21 2019 984 221 4279 83
Fragments arranged by size:
4279 2019 984 310 221 83 21
SnaBI TAC'GTA
Cuts at: 0 7689 7917
Size: 7689 228
SrfI GCCC'GGGC
Cuts at: 0 3847 7917
Size: 3847 4070
StuI AGG'CCT
Cuts at: 0 3068 4475 6785 7684 7917
Size: 3068 1407 2310 899 233
Fragments arranged by size:
3068 2310 1407 899 233
StyI C'CwwG_G
Cuts at: 0 794 1158 1268 1346 1475 1565 1631 2234
Size: 794 364 110 78 129 90 66 603
Cuts at: 2234 2744 3275 4346 5604 5645 6014 6672 7055
Size: 510 531 1071 1258 41 369 658 383
Cuts at: 7055 7073 7385 7442 7592 7661 7765 7917
Size: 18 312 57 150 69 104 152
Fragments arranged by size:
1258 1071 794 658 603 531 510 383
369 364 312 152 150 129 110 104
90 78 69 66 57 41 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2027 2692 3442 4063 4221 4531 5299 5490
Size: 2027 665 750 621 158 310 768 191
Cuts at: 5490 5617 7216 7917
Size: 127 1599 701
Fragments arranged by size:
2027 1599 768 750 701 665 621 310
191 158 127
TatI w'GTAC_w
Cuts at: 0 1514 2510 2807 3620 3947 3959 4238 4566
Size: 1514 996 297 813 327 12 279 328
Cuts at: 4566 5255 5786 6620 7850 7917
Size: 689 531 834 1230 67
Fragments arranged by size:
1514 1230 996 834 813 689 531 328
327 297 279 67 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 2108 2140 2930 2962 6262 6294
Size: 103 32 1973 32 790 32 3300 32
Cuts at: 6294 7917
Size: 1623
Fragments arranged by size:
3300 1973 1623 790 103 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 5353 7917
Size: 5353 2564
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 885 4476 4692 6103 6349 6637 7392 7653
Size: 885 3591 216 1411 246 288 755 261
Cuts at: 7653 7917
Size: 264
Fragments arranged by size:
3591 1411 885 755 288 264 261 246
216
XhoI C'TCGA_G
Cuts at: 0 331 3334 3555 7917
Size: 331 3003 221 4362
Fragments arranged by size:
4362 3003 331 221
XmaI C'CCGG_G
Cuts at: 0 618 1307 2519 3391 3502 3676 3845 4067
Size: 618 689 1212 872 111 174 169 222
Cuts at: 4067 4657 6764 7623 7917
Size: 590 2107 859 294
Fragments arranged by size:
2107 1212 872 859 689 618 590 294
222 174 169 111
XmnI GAAnn'nnTTC
Cuts at: 0 142 6920 7917
Size: 142 6778 997
Fragments arranged by size:
6778 997 142
ZraI GAC'GTC
Cuts at: 0 2409 2724 7185 7917
Size: 2409 315 4461 732
Fragments arranged by size:
4461 2409 732 315
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
PvuII SalI SanDI SapI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SnaBI SrfI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AgeI AhdI AscI AseI AsiSI BaeI
BciVI BfrBI BmtI BsiWI BspEI BssHII DraI EcoICRI
EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PmeI PpiI
PsiI RsrII SacI SacII SbfI SgfI SgrAI SpeI
SphI SspI SwaI XbaI