Restriction map for
pF1KSDF0400
(Linear) (Six-base) MAPSORT of: FLJ00400.seq Check: 1496 from: 1 to: 2178
FLJ00400.orf
With 182 enzymes: SgfI *
October 24, 2006 15:19 ..
AclI AA'CG_TT
Cuts at: 0 1235 2178
Size: 1235 943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 1564 1779 2178
Size: 1159 405 215 399
Fragments arranged by size:
1159 405 399 215
AgeI A'CCGG_T
Cuts at: 0 1361 2178
Size: 1361 817
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 781 2178
Size: 781 1397
AlwNI CAG_nnn'CTG
Cuts at: 0 249 1014 2178
Size: 249 765 1164
Fragments arranged by size:
1164 765 249
ApaI G_GGCC'C
Cuts at: 0 36 870 1981 2077 2178
Size: 36 834 1111 96 101
Fragments arranged by size:
1111 834 101 96 36
ApoI r'AATT_y
Cuts at: 0 314 2178
Size: 314 1864
AvaI C'yCGr_G
Cuts at: 0 254 863 1962 2049 2178
Size: 254 609 1099 87 129
Fragments arranged by size:
1099 609 254 129 87
BanI G'GyrC_C
Cuts at: 0 42 224 717 1919 2128 2178
Size: 42 182 493 1202 209 50
Fragments arranged by size:
1202 493 209 182 50 42
BanII G_rGCy'C
Cuts at: 0 36 164 269 870 923 1748 1786 1981
Size: 36 128 105 601 53 825 38 195
Cuts at: 1981 2077 2178
Size: 96 101
Fragments arranged by size:
825 601 195 128 105 101 96 53
38 36
BbeI G_GCGC'C
Cuts at: 0 228 721 2178
Size: 228 493 1457
Fragments arranged by size:
1457 493 228
BbsI GAAGACnn'nnnn_
Cuts at: 0 697 729 1494 2178
Size: 697 32 765 684
Fragments arranged by size:
765 697 684 32
BbvCI CC'TCA_GC
Cuts at: 0 619 2178
Size: 619 1559
BglI GCCn_nnn'nGGC
Cuts at: 0 305 874 1347 2178
Size: 305 569 473 831
Fragments arranged by size:
831 569 473 305
BlpI GC'TnA_GC
Cuts at: 0 364 1819 2178
Size: 364 1455 359
Fragments arranged by size:
1455 364 359
Bme1580I G_kGCm'C
Cuts at: 0 36 460 870 1643 1770 1981 2077 2133
Size: 36 424 410 773 127 211 96 56
Cuts at: 2133 2178
Size: 45
Fragments arranged by size:
773 424 410 211 127 96 56 45
36
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 603 1988 2178
Size: 603 1385 190
Fragments arranged by size:
1385 603 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 844 991 1102 1536 1566 2178
Size: 436 408 147 111 434 30 612
Fragments arranged by size:
612 436 434 408 147 111 30
Bpu10I CC'TnA_GC
Cuts at: 0 619 2178
Size: 619 1559
BsaHI Gr'CG_yC
Cuts at: 0 100 225 718 2178
Size: 100 125 493 1460
Fragments arranged by size:
1460 493 125 100
BsaWI w'CCGG_w
Cuts at: 0 1361 1883 2178
Size: 1361 522 295
Fragments arranged by size:
1361 522 295
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 979 1009 1264 1294 1768 1798
Size: 46 30 903 30 255 30 474 30
Cuts at: 1798 1921 1951 1978 2008 2178
Size: 123 30 27 30 170
Fragments arranged by size:
903 474 255 170 123 46 30 30
30 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 70 607 631 732 841 1000 1288 1297
Size: 70 537 24 101 109 159 288 9
Cuts at: 1297 1414 1603 1662 1798 2178
Size: 117 189 59 136 380
Fragments arranged by size:
537 380 288 189 159 136 117 109
101 70 59 24 9
BseYI C'CCAG_C
Cuts at: 0 699 1253 2161 2178
Size: 699 554 908 17
Fragments arranged by size:
908 699 554 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 787 2178
Size: 193 594 1391
Fragments arranged by size:
1391 594 193
BsiEI CG_ry'CG
Cuts at: 0 2071 2178
Size: 2071 107
BsiHKAI G_wGCw'C
Cuts at: 0 1613 2178
Size: 1613 565
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1173 1848 2178
Size: 1173 675 330
Fragments arranged by size:
1173 675 330
Bsp1286I G_dGCh'C
Cuts at: 0 36 164 269 460 870 923 1613 1643
Size: 36 128 105 191 410 53 690 30
Cuts at: 1643 1748 1770 1786 1981 2077 2133 2178
Size: 105 22 16 195 96 56 45
Fragments arranged by size:
690 410 195 191 128 105 105 96
56 53 45 36 30 22 16
BspEI T'CCGG_A
Cuts at: 0 1883 2178
Size: 1883 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 368 905 1022 1893 2178
Size: 368 537 117 871 285
Fragments arranged by size:
871 537 368 285 117
BsrBI CCG'CTC
Cuts at: 0 30 81 2178
Size: 30 51 2097
Fragments arranged by size:
2097 51 30
BsrFI r'CCGG_y
Cuts at: 0 203 1361 1373 2178
Size: 203 1158 12 805
Fragments arranged by size:
1158 805 203 12
BsrGI T'GTAC_A
Cuts at: 0 593 1280 2178
Size: 593 687 898
Fragments arranged by size:
898 687 593
BstAPI GCAn_nnn'nTGC
Cuts at: 0 180 2178
Size: 180 1998
BstEII G'GTnAC_C
Cuts at: 0 1332 1449 2178
Size: 1332 117 729
Fragments arranged by size:
1332 729 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 460 2178
Size: 460 1718
BtgI C'CryG_G
Cuts at: 0 10 164 1265 1313 2065 2093 2178
Size: 10 154 1101 48 752 28 85
Fragments arranged by size:
1101 752 154 85 48 28 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1014 2178
Size: 1014 1164
BtsI GCAGTG_nn'
Cuts at: 0 1643 2178
Size: 1643 535
DraIII CAC_nnn'GTG
Cuts at: 0 1926 2178
Size: 1926 252
EaeI y'GGCC_r
Cuts at: 0 1145 1758 2068 2178
Size: 1145 613 310 110
Fragments arranged by size:
1145 613 310 110
EagI C'GGCC_G
Cuts at: 0 2068 2178
Size: 2068 110
EarI CTCTTCn'nnn_
Cuts at: 0 307 556 1471 1513 1696 2178
Size: 307 249 915 42 183 482
Fragments arranged by size:
915 482 307 249 183 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 592 681 2130 2178
Size: 403 189 89 1449 48
Fragments arranged by size:
1449 403 189 89 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 844 991 1102 1159 1536 1564 1566
Size: 436 408 147 111 57 377 28 2
Cuts at: 1566 1779 2178
Size: 213 399
Fragments arranged by size:
436 408 399 377 213 147 111 57
28 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 425 1927 2178
Size: 232 193 1502 251
Fragments arranged by size:
1502 251 232 193
EcoO109I rG'GnC_Cy
Cuts at: 0 33 258 297 430 530 1051 1932 1978
Size: 33 225 39 133 100 521 881 46
Cuts at: 1978 2061 2074 2178
Size: 83 13 104
Fragments arranged by size:
881 521 225 133 104 100 83 46
39 33 13
EcoRI G'AATT_C
Cuts at: 0 314 2178
Size: 314 1864
HaeII r_GCGC'y
Cuts at: 0 141 204 228 283 721 731 947 2178
Size: 141 63 24 55 438 10 216 1231
Fragments arranged by size:
1231 438 216 141 63 55 24 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 1921 1953 2178
Size: 1351 32 538 32 225
Fragments arranged by size:
1351 538 225 32 32
HincII GTy'rAC
Cuts at: 0 906 1041 1110 2178
Size: 906 135 69 1068
Fragments arranged by size:
1068 906 135 69
HindIII A'AGCT_T
Cuts at: 0 384 2000 2178
Size: 384 1616 178
Fragments arranged by size:
1616 384 178
KasI G'GCGC_C
Cuts at: 0 224 717 2178
Size: 224 493 1461
Fragments arranged by size:
1461 493 224
MscI TGG'CCA
Cuts at: 0 1147 1760 2178
Size: 1147 613 418
Fragments arranged by size:
1147 613 418
MslI CAynn'nnrTG
Cuts at: 0 763 1456 2178
Size: 763 693 722
Fragments arranged by size:
763 722 693
MspA1I CmG'CkG
Cuts at: 0 153 166 192 246 627 846 1080 1214
Size: 153 13 26 54 381 219 234 134
Cuts at: 1214 1893 2067 2095 2165 2178
Size: 679 174 28 70 13
Fragments arranged by size:
679 381 234 219 174 153 134 70
54 28 26 13 13
NaeI GCC'GGC
Cuts at: 0 205 2178
Size: 205 1973
NarI GG'CG_CC
Cuts at: 0 225 718 2178
Size: 225 493 1460
Fragments arranged by size:
1460 493 225
NgoMIV G'CCGG_C
Cuts at: 0 203 2178
Size: 203 1975
NruI TCG'CGA
Cuts at: 0 1941 2178
Size: 1941 237
NspI r_CATG'y
Cuts at: 0 517 2178
Size: 517 1661
PasI CC'CwG_GG
Cuts at: 0 37 1021 1173 1644 2178
Size: 37 984 152 471 534
Fragments arranged by size:
984 534 471 152 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 953 1779 2041 2178
Size: 953 826 262 137
Fragments arranged by size:
953 826 262 137
PfoI T'CCnGG_A
Cuts at: 0 1724 2178
Size: 1724 454
PpuMI rG'GwC_Cy
Cuts at: 0 1051 1932 2178
Size: 1051 881 246
Fragments arranged by size:
1051 881 246
PspOMI G'GGCC_C
Cuts at: 0 32 866 1977 2073 2178
Size: 32 834 1111 96 105
Fragments arranged by size:
1111 834 105 96 32
PstI C_TGCA'G
Cuts at: 0 1638 2178
Size: 1638 540
PvuII CAG'CTG
Cuts at: 0 153 846 1080 1214 1893 2178
Size: 153 693 234 134 679 285
Fragments arranged by size:
693 679 285 234 153 134
SacII CC_GC'GG
Cuts at: 0 167 2068 2096 2178
Size: 167 1901 28 82
Fragments arranged by size:
1901 167 82 28
SanDI GG'GwC_CC
Cuts at: 0 1051 2178
Size: 1051 1127
ScaI AGT'ACT
Cuts at: 0 967 2178
Size: 967 1211
SexAI A'CCwGG_T
Cuts at: 0 1922 2178
Size: 1922 256
SfcI C'TryA_G
Cuts at: 0 1634 2178
Size: 1634 544
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 874 2178
Size: 874 1304
SfoI GGC'GCC
Cuts at: 0 226 719 2178
Size: 226 493 1459
Fragments arranged by size:
1459 493 226
SgrAI Cr'CCGG_yG
Cuts at: 0 203 2178
Size: 203 1975
SmaI CCC'GGG
Cuts at: 0 256 865 2051 2178
Size: 256 609 1186 127
Fragments arranged by size:
1186 609 256 127
StyI C'CwwG_G
Cuts at: 0 261 731 1740 2178
Size: 261 470 1009 438
Fragments arranged by size:
1009 470 438 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 308 2178
Size: 308 1870
TatI w'GTAC_w
Cuts at: 0 593 965 1280 1532 2178
Size: 593 372 315 252 646
Fragments arranged by size:
646 593 372 315 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 2178
Size: 46 32 2100
Fragments arranged by size:
2100 46 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 478 1422 2178
Size: 478 944 756
Fragments arranged by size:
944 756 478
XmaI C'CCGG_G
Cuts at: 0 254 863 2049 2178
Size: 254 609 1186 129
Fragments arranged by size:
1186 609 254 129
Enzymes that do cut:
AclI AcuI AgeI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I
BplI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrGI BstAPI BstEII BstXI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII HindIII KasI MscI MslI
MspA1I NaeI NarI NgoMIV NruI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacII SanDI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI StyI TaqII
TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BpuEI BsaI BsaAI BsaBI BsiWI BsmI BspHI
BsrDI BssHII BssSI BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EcoICRI EcoRV FalI FseI FspI
FspAI HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NotI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI
SapI SbfI SgfI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI