Restriction map for
pF1KF0405
(Linear) (Six-base) MAPSORT of: FLJ00405.seq Check: 445 from: 1 to: 1206
FLJ00405.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 577 1206
Size: 577 629
AccI GT'mk_AC
Cuts at: 0 368 1206
Size: 368 838
AclI AA'CG_TT
Cuts at: 0 96 1206
Size: 96 1110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 324 560 640 1206
Size: 287 37 236 80 566
Fragments arranged by size:
566 287 236 80 37
AlwNI CAG_nnn'CTG
Cuts at: 0 618 1206
Size: 618 588
ApaLI G'TGCA_C
Cuts at: 0 376 1206
Size: 376 830
ApoI r'AATT_y
Cuts at: 0 720 1206
Size: 720 486
BanII G_rGCy'C
Cuts at: 0 10 1206
Size: 10 1196
BbsI GAAGACnn'nnnn_
Cuts at: 0 530 1001 1206
Size: 530 471 205
Fragments arranged by size:
530 471 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 336 370 1206
Size: 336 34 836
Fragments arranged by size:
836 336 34
BfrBI ATG'CAT
Cuts at: 0 190 949 1206
Size: 190 759 257
Fragments arranged by size:
759 257 190
BglI GCCn_nnn'nGGC
Cuts at: 0 799 1206
Size: 799 407
Bme1580I G_kGCm'C
Cuts at: 0 380 1206
Size: 380 826
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 128 1206
Size: 128 1078
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 484 1206
Size: 477 7 722
Fragments arranged by size:
722 477 7
BsaAI yAC'GTr
Cuts at: 0 729 798 1206
Size: 729 69 408
Fragments arranged by size:
729 408 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 744 774 844 874 1206
Size: 744 30 70 30 332
Fragments arranged by size:
744 332 70 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 746 749 752 1206
Size: 746 3 3 454
Fragments arranged by size:
746 454 3 3
BseYI C'CCAG_C
Cuts at: 0 277 1192 1206
Size: 277 915 14
Fragments arranged by size:
915 277 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 1206
Size: 434 772
BsiHKAI G_wGCw'C
Cuts at: 0 380 1206
Size: 380 826
BsmI GAATG_Cn'
Cuts at: 0 1138 1206
Size: 1138 68
BsmBI CGTCTCn'nnnn_
Cuts at: 0 41 1206
Size: 41 1165
Bsp1286I G_dGCh'C
Cuts at: 0 10 380 1206
Size: 10 370 826
Fragments arranged by size:
826 370 10
BspHI T'CATG_A
Cuts at: 0 765 1206
Size: 765 441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 226 577 1206
Size: 226 351 629
Fragments arranged by size:
629 351 226
BsrGI T'GTAC_A
Cuts at: 0 1026 1206
Size: 1026 180
BstBI TT'CG_AA
Cuts at: 0 237 1206
Size: 237 969
BstXI CCAn_nnnn'nTGG
Cuts at: 0 676 1206
Size: 676 530
BstYI r'GATC_y
Cuts at: 0 488 1206
Size: 488 718
BtgI C'CryG_G
Cuts at: 0 1014 1206
Size: 1014 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1135 1206
Size: 1135 71
BtsI GCAGTG_nn'
Cuts at: 0 422 1206
Size: 422 784
DraI TTT'AAA
Cuts at: 0 408 630 1206
Size: 408 222 576
Fragments arranged by size:
576 408 222
EaeI y'GGCC_r
Cuts at: 0 1017 1206
Size: 1017 189
EarI CTCTTCn'nnn_
Cuts at: 0 266 343 1206
Size: 266 77 863
Fragments arranged by size:
863 266 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 324 477 484 560 640 1206
Size: 287 37 153 7 76 80 566
Fragments arranged by size:
566 287 153 80 76 37 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 1206
Size: 844 32 330
Fragments arranged by size:
844 330 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 657 1206
Size: 657 549
MspA1I CmG'CkG
Cuts at: 0 434 1196 1206
Size: 434 762 10
Fragments arranged by size:
762 434 10
NruI TCG'CGA
Cuts at: 0 1154 1206
Size: 1154 52
NsiI A_TGCA'T
Cuts at: 0 192 951 1206
Size: 192 759 255
Fragments arranged by size:
759 255 192
PfoI T'CCnGG_A
Cuts at: 0 952 1206
Size: 952 254
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1206
Size: 265 32 909
Fragments arranged by size:
909 265 32
PvuII CAG'CTG
Cuts at: 0 434 1196 1206
Size: 434 762 10
Fragments arranged by size:
762 434 10
SapI GCTCTTCn'nnn_
Cuts at: 0 343 1206
Size: 343 863
SfcI C'TryA_G
Cuts at: 0 879 1206
Size: 879 327
StyI C'CwwG_G
Cuts at: 0 869 1206
Size: 869 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 891 1206
Size: 891 315
TatI w'GTAC_w
Cuts at: 0 227 1026 1206
Size: 227 799 180
Fragments arranged by size:
799 227 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 706 738 909 941 1206
Size: 706 32 171 32 265
Fragments arranged by size:
706 265 171 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 950 1027 1206
Size: 950 77 179
Fragments arranged by size:
950 179 77
XmnI GAAnn'nnTTC
Cuts at: 0 608 1206
Size: 608 598
Enzymes that do cut:
AarI AccI AclI AcuI AlwNI ApaLI ApoI BanII
BbsI BcgI BfrBI BglI Bme1580I BplI BpmI BsaAI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrGI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI EaeI EarI Eco57MI Hin4I MmeI MspA1I
NruI NsiI PfoI PpiI PvuII SapI SfcI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BglII
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsiEI BsiWI BspEI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI