Restriction map for
pF1KF0414
(Linear) (Six-base) MAPSORT of: FLJ00414.seq Check: 9868 from: 1 to: 3174
FLJ00414.orf
With 182 enzymes: SgfI *
April 24, 2009 13:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 333 1479 3072 3174
Size: 333 1146 1593 102
Fragments arranged by size:
1593 1146 333 102
AatII G_ACGT'C
Cuts at: 0 737 3174
Size: 737 2437
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1229 1330 1485 2088 3174
Size: 82 1147 101 155 603 1086
Fragments arranged by size:
1147 1086 603 155 101 82
AfeI AGC'GCT
Cuts at: 0 334 3174
Size: 334 2840
AflIII A'CryG_T
Cuts at: 0 611 2130 2132 3174
Size: 611 1519 2 1042
Fragments arranged by size:
1519 1042 611 2
AhdI GACnn_n'nnGTC
Cuts at: 0 1221 3174
Size: 1221 1953
AleI CACnn'nnGTG
Cuts at: 0 809 3174
Size: 809 2365
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2131 3174
Size: 2131 1043
AlwNI CAG_nnn'CTG
Cuts at: 0 60 132 534 843 1527 2214 3174
Size: 60 72 402 309 684 687 960
Fragments arranged by size:
960 687 684 402 309 72 60
ApaI G_GGCC'C
Cuts at: 0 674 1416 2444 2480 2715 2736 2925 3174
Size: 674 742 1028 36 235 21 189 249
Fragments arranged by size:
1028 742 674 249 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 0 511 691 2014 3174
Size: 511 180 1323 1160
Fragments arranged by size:
1323 1160 511 180
AvaI C'yCGr_G
Cuts at: 0 373 1356 1409 1798 2437 2495 2569 2667
Size: 373 983 53 389 639 58 74 98
Cuts at: 2667 2708 2714 2766 2865 3174
Size: 41 6 52 99 309
Fragments arranged by size:
983 639 389 373 309 99 98 74
58 53 52 41 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2119 2152 3174
Size: 2119 33 1022
Fragments arranged by size:
2119 1022 33
BamHI G'GATC_C
Cuts at: 0 2312 2614 3174
Size: 2312 302 560
Fragments arranged by size:
2312 560 302
BanI G'GyrC_C
Cuts at: 0 2430 2505 2902 3174
Size: 2430 75 397 272
Fragments arranged by size:
2430 397 272 75
BanII G_rGCy'C
Cuts at: 0 475 674 686 1416 1928 2444 2480 2533
Size: 475 199 12 730 512 516 36 53
Cuts at: 2533 2715 2736 2925 3174
Size: 182 21 189 249
Fragments arranged by size:
730 516 512 475 249 199 189 182
53 36 21 12
BbeI G_GCGC'C
Cuts at: 0 2509 3174
Size: 2509 665
BbsI GAAGACnn'nnnn_
Cuts at: 0 1399 3174
Size: 1399 1775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 921 955 3174
Size: 921 34 2219
Fragments arranged by size:
2219 921 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3131 3174
Size: 3131 43
BfrBI ATG'CAT
Cuts at: 0 1057 2597 3174
Size: 1057 1540 577
Fragments arranged by size:
1540 1057 577
BglI GCCn_nnn'nGGC
Cuts at: 0 2225 2366 2439 3174
Size: 2225 141 73 735
Fragments arranged by size:
2225 735 141 73
BlpI GC'TnA_GC
Cuts at: 0 535 3174
Size: 535 2639
Bme1580I G_kGCm'C
Cuts at: 0 515 674 695 1416 2018 2435 2444 2480
Size: 515 159 21 721 602 417 9 36
Cuts at: 2480 2715 2736 2925 3174
Size: 235 21 189 249
Fragments arranged by size:
721 602 515 417 249 235 189 159
36 21 21 9
BmgBI CAC'GTC
Cuts at: 0 1218 3174
Size: 1218 1956
BmrI ACTGGGnnnn_n'
Cuts at: 0 591 775 831 2737 3174
Size: 591 184 56 1906 437
Fragments arranged by size:
1906 591 437 184 56
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1266 1593 2826 2832 2983 3174
Size: 1266 327 1233 6 151 191
Fragments arranged by size:
1266 1233 327 191 151 6
Bpu10I CC'TnA_GC
Cuts at: 0 106 497 3174
Size: 106 391 2677
Fragments arranged by size:
2677 391 106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 1726 1792 3174
Size: 910 816 66 1382
Fragments arranged by size:
1382 910 816 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 1501 1990 3174
Size: 1501 489 1184
Fragments arranged by size:
1501 1184 489
BsaAI yAC'GTr
Cuts at: 0 1737 2133 3174
Size: 1737 396 1041
Fragments arranged by size:
1737 1041 396
BsaHI Gr'CG_yC
Cuts at: 0 734 1477 2506 3174
Size: 734 743 1029 668
Fragments arranged by size:
1029 743 734 668
BsaWI w'CCGG_w
Cuts at: 0 1826 2454 3174
Size: 1826 628 720
Fragments arranged by size:
1826 720 628
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1141 1171 1462 1492 1576 1606 2389 2419
Size: 1141 30 291 30 84 30 783 30
Cuts at: 2419 2799 2829 3174
Size: 380 30 345
Fragments arranged by size:
1141 783 380 345 291 84 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1033 1345 1581 2008 2980 3174
Size: 1033 312 236 427 972 194
Fragments arranged by size:
1033 972 427 312 236 194
BseYI C'CCAG_C
Cuts at: 0 216 438 662 960 1872 2275 2463 2578
Size: 216 222 224 298 912 403 188 115
Cuts at: 2578 2662 3049 3174
Size: 84 387 125
Fragments arranged by size:
912 403 387 298 224 222 216 188
125 115 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 3174
Size: 118 3056
BsiHKAI G_wGCw'C
Cuts at: 0 503 515 695 1826 2018 3104 3110 3174
Size: 503 12 180 1131 192 1086 6 64
Fragments arranged by size:
1131 1086 503 192 180 64 12 6
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1167 3174
Size: 1167 2007
Bsp1286I G_dGCh'C
Cuts at: 0 475 503 515 674 686 695 1416 1826
Size: 475 28 12 159 12 9 721 410
Cuts at: 1826 1928 2018 2435 2444 2480 2533 2715 2736
Size: 102 90 417 9 36 53 182 21
Cuts at: 2736 2925 3104 3110 3174
Size: 189 179 6 64
Fragments arranged by size:
721 475 417 410 189 182 179 159
102 90 64 53 36 28 21 12
12 9 9 6
BspEI T'CCGG_A
Cuts at: 0 1826 2454 3174
Size: 1826 628 720
Fragments arranged by size:
1826 720 628
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 161 284 333 1479 2199 2457 3072 3174
Size: 161 123 49 1146 720 258 615 102
Fragments arranged by size:
1146 720 615 258 161 123 102 49
BsrBI CCG'CTC
Cuts at: 0 2655 3174
Size: 2655 519
BsrDI GCAATG_nn'
Cuts at: 0 1260 3174
Size: 1260 1914
BsrFI r'CCGG_y
Cuts at: 0 4 1539 1642 2623 2998 3174
Size: 4 1535 103 981 375 176
Fragments arranged by size:
1535 981 375 176 103 4
BssSI C'ACGA_G
Cuts at: 0 574 940 3095 3103 3109 3174
Size: 574 366 2155 8 6 65
Fragments arranged by size:
2155 574 366 65 8 6
BstAPI GCAn_nnn'nTGC
Cuts at: 0 132 1821 3174
Size: 132 1689 1353
Fragments arranged by size:
1689 1353 132
BstEII G'GTnAC_C
Cuts at: 0 580 3059 3174
Size: 580 2479 115
Fragments arranged by size:
2479 580 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1446 2415 3174
Size: 1446 969 759
Fragments arranged by size:
1446 969 759
BstYI r'GATC_y
Cuts at: 0 719 935 2312 2614 3174
Size: 719 216 1377 302 560
Fragments arranged by size:
1377 719 560 302 216
Bsu36I CC'TnA_GG
Cuts at: 0 2897 3174
Size: 2897 277
BtgI C'CryG_G
Cuts at: 0 696 1445 1881 3124 3138 3174
Size: 696 749 436 1243 14 36
Fragments arranged by size:
1243 749 696 436 36 14
BtsI GCAGTG_nn'
Cuts at: 0 804 816 894 1085 3174
Size: 804 12 78 191 2089
Fragments arranged by size:
2089 804 191 78 12
DraIII CAC_nnn'GTG
Cuts at: 0 510 3174
Size: 510 2664
EaeI y'GGCC_r
Cuts at: 0 2 1257 1319 1448 1839 2085 2327 2585
Size: 2 1255 62 129 391 246 242 258
Cuts at: 2585 2656 3174
Size: 71 518
Fragments arranged by size:
1255 518 391 258 246 242 129 71
62 2
EarI CTCTTCn'nnn_
Cuts at: 0 221 730 1205 2147 2236 2521 3174
Size: 221 509 475 942 89 285 653
Fragments arranged by size:
942 653 509 475 285 221 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1698 2985 3174
Size: 1698 1287 189
Fragments arranged by size:
1698 1287 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1229 1266 1330 1485 1593 2088 2826
Size: 82 1147 37 64 155 108 495 738
Cuts at: 2826 2832 2983 3174
Size: 6 151 191
Fragments arranged by size:
1147 738 495 191 155 151 108 82
64 37 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 943 3174
Size: 943 2231
EcoO109I rG'GnC_Cy
Cuts at: 0 120 267 624 1143 1413 1788 2476 2640
Size: 120 147 357 519 270 375 688 164
Cuts at: 2640 2671 2732 2733 2784 2794 2801 2882 2921
Size: 31 61 1 51 10 7 81 39
Cuts at: 2921 2922 3174
Size: 1 252
Fragments arranged by size:
688 519 375 357 270 252 164 147
120 81 61 51 39 31 10 7
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 741 3027 3174
Size: 741 2286 147
Fragments arranged by size:
2286 741 147
HaeII r_GCGC'y
Cuts at: 0 336 1718 2336 2509 3174
Size: 336 1382 618 173 665
Fragments arranged by size:
1382 665 618 336 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 1207 1239 1462 1494 2389 2421
Size: 862 32 313 32 223 32 895 32
Cuts at: 2421 2799 2831 3174
Size: 378 32 343
Fragments arranged by size:
895 862 378 343 313 223 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1275 3174
Size: 1275 1899
KasI G'GCGC_C
Cuts at: 0 2505 3174
Size: 2505 669
MscI TGG'CCA
Cuts at: 0 1450 1841 2087 3174
Size: 1450 391 246 1087
Fragments arranged by size:
1450 1087 391 246
MslI CAynn'nnrTG
Cuts at: 0 314 506 809 1465 2167 2596 3174
Size: 314 192 303 656 702 429 578
Fragments arranged by size:
702 656 578 429 314 303 192
MspA1I CmG'CkG
Cuts at: 0 129 534 644 1484 1695 1818 2195 2310
Size: 129 405 110 840 211 123 377 115
Cuts at: 2310 2486 2512 2891 3174
Size: 176 26 379 283
Fragments arranged by size:
840 405 379 377 283 211 176 129
123 115 110 26
NaeI GCC'GGC
Cuts at: 0 6 1541 1644 2625 3174
Size: 6 1535 103 981 549
Fragments arranged by size:
1535 981 549 103 6
NarI GG'CG_CC
Cuts at: 0 2506 3174
Size: 2506 668
NcoI C'CATG_G
Cuts at: 0 696 3174
Size: 696 2478
NgoMIV G'CCGG_C
Cuts at: 0 4 1539 1642 2623 3174
Size: 4 1535 103 981 551
Fragments arranged by size:
1535 981 551 103 4
NsiI A_TGCA'T
Cuts at: 0 1059 2599 3174
Size: 1059 1540 575
Fragments arranged by size:
1540 1059 575
NspI r_CATG'y
Cuts at: 0 615 1758 2022 2176 2597 3174
Size: 615 1143 264 154 421 577
Fragments arranged by size:
1143 615 577 421 264 154
PciI A'CATG_T
Cuts at: 0 611 3174
Size: 611 2563
PfoI T'CCnGG_A
Cuts at: 0 2372 2558 3174
Size: 2372 186 616
Fragments arranged by size:
2372 616 186
PmlI CAC'GTG
Cuts at: 0 2133 3174
Size: 2133 1041
PpuMI rG'GwC_Cy
Cuts at: 0 120 267 624 1143 1788 2794 2882 3174
Size: 120 147 357 519 645 1006 88 292
Fragments arranged by size:
1006 645 519 357 292 147 120 88
PspOMI G'GGCC_C
Cuts at: 0 670 1412 2440 2476 2711 2732 2921 3174
Size: 670 742 1028 36 235 21 189 253
Fragments arranged by size:
1028 742 670 253 235 189 36 21
PstI C_TGCA'G
Cuts at: 0 128 332 344 632 811 1532 1694 1942
Size: 128 204 12 288 179 721 162 248
Cuts at: 1942 2648 2911 2930 3174
Size: 706 263 19 244
Fragments arranged by size:
721 706 288 263 248 244 204 179
162 128 19 12
PvuII CAG'CTG
Cuts at: 0 129 1484 1695 1818 2512 3174
Size: 129 1355 211 123 694 662
Fragments arranged by size:
1355 694 662 211 129 123
SapI GCTCTTCn'nnn_
Cuts at: 0 2521 3174
Size: 2521 653
SbfI CC_TGCA'GG
Cuts at: 0 344 632 2911 2930 3174
Size: 344 288 2279 19 244
Fragments arranged by size:
2279 344 288 244 19
SexAI A'CCwGG_T
Cuts at: 0 1424 3174
Size: 1424 1750
SfcI C'TryA_G
Cuts at: 0 124 291 328 340 628 807 1528 1690
Size: 124 167 37 12 288 179 721 162
Cuts at: 1690 1938 2124 2644 2907 2926 3174
Size: 248 186 520 263 19 248
Fragments arranged by size:
721 520 288 263 248 248 186 179
167 162 124 37 19 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2225 3174
Size: 2225 949
SfoI GGC'GCC
Cuts at: 0 2507 3174
Size: 2507 667
SgrAI Cr'CCGG_yG
Cuts at: 0 2623 3174
Size: 2623 551
SmaI CCC'GGG
Cuts at: 0 1358 1411 1800 2439 2571 2669 2710 2716
Size: 1358 53 389 639 132 98 41 6
Cuts at: 2716 2768 2867 3174
Size: 52 99 307
Fragments arranged by size:
1358 639 389 307 132 99 98 53
52 41 6
SmlI C'TyrA_G
Cuts at: 0 373 925 1741 1807 3174
Size: 373 552 816 66 1367
Fragments arranged by size:
1367 816 552 373 66
SnaBI TAC'GTA
Cuts at: 0 1737 3174
Size: 1737 1437
SrfI GCCC'GGGC
Cuts at: 0 1800 2716 2867 3174
Size: 1800 916 151 307
Fragments arranged by size:
1800 916 307 151
StuI AGG'CCT
Cuts at: 0 2158 2228 3174
Size: 2158 70 946
Fragments arranged by size:
2158 946 70
StyI C'CwwG_G
Cuts at: 0 207 696 855 1511 2408 2789 3154 3174
Size: 207 489 159 656 897 381 365 20
Fragments arranged by size:
897 656 489 381 365 207 159 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 568 1468 1739 2676 3174
Size: 568 900 271 937 498
Fragments arranged by size:
937 900 568 498 271
TatI w'GTAC_w
Cuts at: 0 1433 3174
Size: 1433 1741
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1821 1853 3174
Size: 1821 32 1321
Fragments arranged by size:
1821 1321 32
Tth111I GACn'n_nGTC
Cuts at: 0 3087 3174
Size: 3087 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2065 3174
Size: 2065 1109
XhoI C'TCGA_G
Cuts at: 0 373 3174
Size: 373 2801
XmaI C'CCGG_G
Cuts at: 0 1356 1409 1798 2437 2569 2667 2708 2714
Size: 1356 53 389 639 132 98 41 6
Cuts at: 2714 2766 2865 3174
Size: 52 99 309
Fragments arranged by size:
1356 639 389 309 132 99 98 53
52 41 6
ZraI GAC'GTC
Cuts at: 0 735 3174
Size: 735 2439
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmlI PpuMI PspOMI PstI
PvuII SapI SbfI SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AloI ApoI AscI
AseI AsiSI AvrII BbvCI BclI BglII BmtI BplI
BsaBI BsiEI BsiWI BsmI BspHI BsrGI BssHII BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI
MfeI MluI MmeI NdeI NheI NotI NruI PacI
PasI PflMI PmeI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI ScaI SgfI
SpeI SphI SspI SwaI XbaI XmnI