Restriction map for
pF1KF0417
(Linear) (Six-base) MAPSORT of: FLJ00417.seq Check: 5040 from: 1 to: 1617
FLJ00417.orf
With 182 enzymes: SgfI *
September 25, 2009 15:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 68 1515 1617
Size: 68 1447 102
Fragments arranged by size:
1447 102 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 531 1617
Size: 531 1086
AfeI AGC'GCT
Cuts at: 0 319 1617
Size: 319 1298
AflIII A'CryG_T
Cuts at: 0 573 575 1617
Size: 573 2 1042
Fragments arranged by size:
1042 573 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1617
Size: 574 1043
AlwNI CAG_nnn'CTG
Cuts at: 0 657 1617
Size: 657 960
ApaI G_GGCC'C
Cuts at: 0 887 923 1158 1179 1368 1617
Size: 887 36 235 21 189 249
Fragments arranged by size:
887 249 235 189 36 21
ApaLI G'TGCA_C
Cuts at: 0 457 1617
Size: 457 1160
AvaI C'yCGr_G
Cuts at: 0 880 938 1012 1110 1151 1157 1209 1308
Size: 880 58 74 98 41 6 52 99
Cuts at: 1308 1617
Size: 309
Fragments arranged by size:
880 309 99 98 74 58 52 41
6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 562 595 1617
Size: 562 33 1022
Fragments arranged by size:
1022 562 33
BamHI G'GATC_C
Cuts at: 0 66 755 1057 1617
Size: 66 689 302 560
Fragments arranged by size:
689 560 302 66
BanI G'GyrC_C
Cuts at: 0 130 873 948 1345 1617
Size: 130 743 75 397 272
Fragments arranged by size:
743 397 272 130 75
BanII G_rGCy'C
Cuts at: 0 371 887 923 976 1158 1179 1368 1617
Size: 371 516 36 53 182 21 189 249
Fragments arranged by size:
516 371 249 189 182 53 36 21
BbeI G_GCGC'C
Cuts at: 0 952 1617
Size: 952 665
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 1617
Size: 303 1314
BciVI GTATCCnnnnn_n'
Cuts at: 0 1574 1617
Size: 1574 43
BfrBI ATG'CAT
Cuts at: 0 1040 1617
Size: 1040 577
BglI GCCn_nnn'nGGC
Cuts at: 0 668 809 882 1617
Size: 668 141 73 735
Fragments arranged by size:
735 668 141 73
BglII A'GATC_T
Cuts at: 0 260 1617
Size: 260 1357
Bme1580I G_kGCm'C
Cuts at: 0 461 878 887 923 1158 1179 1368 1617
Size: 461 417 9 36 235 21 189 249
Fragments arranged by size:
461 417 249 235 189 36 21 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1180 1617
Size: 1180 437
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 231 1617
Size: 231 1386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1269 1275 1426 1617
Size: 249 1020 6 151 191
Fragments arranged by size:
1020 249 191 151 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1617
Size: 111 1506
BsaI GGTCTCn'nnnn_
Cuts at: 0 433 1617
Size: 433 1184
BsaAI yAC'GTr
Cuts at: 0 576 1617
Size: 576 1041
BsaBI GATnn'nnATC
Cuts at: 0 265 1617
Size: 265 1352
BsaHI Gr'CG_yC
Cuts at: 0 949 1617
Size: 949 668
BsaWI w'CCGG_w
Cuts at: 0 69 897 1617
Size: 69 828 720
Fragments arranged by size:
828 720 69
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 832 862 1242 1272 1617
Size: 64 30 738 30 380 30 345
Fragments arranged by size:
738 380 345 64 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 1423 1617
Size: 451 972 194
Fragments arranged by size:
972 451 194
BseYI C'CCAG_C
Cuts at: 0 36 718 906 1021 1105 1492 1617
Size: 36 682 188 115 84 387 125
Fragments arranged by size:
682 387 188 125 115 84 36
BsiHKAI G_wGCw'C
Cuts at: 0 212 461 1547 1553 1617
Size: 212 249 1086 6 64
Fragments arranged by size:
1086 249 212 64 6
Bsp1286I G_dGCh'C
Cuts at: 0 212 371 461 878 887 923 976 1158
Size: 212 159 90 417 9 36 53 182
Cuts at: 1158 1179 1368 1547 1553 1617
Size: 21 189 179 6 64
Fragments arranged by size:
417 212 189 182 179 159 90 64
53 36 21 9 6
BspEI T'CCGG_A
Cuts at: 0 69 897 1617
Size: 69 828 720
Fragments arranged by size:
828 720 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 900 1515 1617
Size: 68 832 615 102
Fragments arranged by size:
832 615 102 68
BsrBI CCG'CTC
Cuts at: 0 120 215 1098 1617
Size: 120 95 883 519
Fragments arranged by size:
883 519 120 95
BsrFI r'CCGG_y
Cuts at: 0 19 1066 1441 1617
Size: 19 1047 375 176
Fragments arranged by size:
1047 375 176 19
BssSI C'ACGA_G
Cuts at: 0 1538 1546 1552 1617
Size: 1538 8 6 65
Fragments arranged by size:
1538 65 8 6
BstEII G'GTnAC_C
Cuts at: 0 1502 1617
Size: 1502 115
BstXI CCAn_nnnn'nTGG
Cuts at: 0 183 858 1617
Size: 183 675 759
Fragments arranged by size:
759 675 183
BstYI r'GATC_y
Cuts at: 0 66 260 755 1057 1617
Size: 66 194 495 302 560
Fragments arranged by size:
560 495 302 194 66
Bsu36I CC'TnA_GG
Cuts at: 0 50 1340 1617
Size: 50 1290 277
Fragments arranged by size:
1290 277 50
BtgI C'CryG_G
Cuts at: 0 110 1567 1581 1617
Size: 110 1457 14 36
Fragments arranged by size:
1457 110 36 14
EaeI y'GGCC_r
Cuts at: 0 528 770 1028 1099 1617
Size: 528 242 258 71 518
Fragments arranged by size:
528 518 258 242 71
EarI CTCTTCn'nnn_
Cuts at: 0 47 590 679 964 1617
Size: 47 543 89 285 653
Fragments arranged by size:
653 543 285 89 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 1428 1617
Size: 35 1393 189
Fragments arranged by size:
1393 189 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 531 1269 1275 1426 1617
Size: 249 282 738 6 151 191
Fragments arranged by size:
738 282 249 191 151 6
EcoO109I rG'GnC_Cy
Cuts at: 0 54 180 233 919 1083 1114 1175 1176
Size: 54 126 53 686 164 31 61 1
Cuts at: 1176 1227 1237 1244 1325 1364 1365 1617
Size: 51 10 7 81 39 1 252
Fragments arranged by size:
686 252 164 126 81 61 54 53
51 39 31 10 7 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1470 1617
Size: 1470 147
HaeII r_GCGC'y
Cuts at: 0 98 321 779 952 1617
Size: 98 223 458 173 665
Fragments arranged by size:
665 458 223 173 98
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 832 864 1242 1274 1617
Size: 832 32 378 32 343
Fragments arranged by size:
832 378 343 32 32
KasI G'GCGC_C
Cuts at: 0 948 1617
Size: 948 669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1617
Size: 284 1333
MscI TGG'CCA
Cuts at: 0 530 1617
Size: 530 1087
MslI CAynn'nnrTG
Cuts at: 0 610 1039 1617
Size: 610 429 578
Fragments arranged by size:
610 578 429
MspA1I CmG'CkG
Cuts at: 0 227 638 753 929 955 1334 1617
Size: 227 411 115 176 26 379 283
Fragments arranged by size:
411 379 283 227 176 115 26
NaeI GCC'GGC
Cuts at: 0 21 1068 1617
Size: 21 1047 549
Fragments arranged by size:
1047 549 21
NarI GG'CG_CC
Cuts at: 0 949 1617
Size: 949 668
NgoMIV G'CCGG_C
Cuts at: 0 19 1066 1617
Size: 19 1047 551
Fragments arranged by size:
1047 551 19
NsiI A_TGCA'T
Cuts at: 0 1042 1617
Size: 1042 575
NspI r_CATG'y
Cuts at: 0 465 619 1040 1617
Size: 465 154 421 577
Fragments arranged by size:
577 465 421 154
PasI CC'CwG_GG
Cuts at: 0 58 1617
Size: 58 1559
PfoI T'CCnGG_A
Cuts at: 0 278 815 1001 1617
Size: 278 537 186 616
Fragments arranged by size:
616 537 278 186
PmlI CAC'GTG
Cuts at: 0 576 1617
Size: 576 1041
PpuMI rG'GwC_Cy
Cuts at: 0 54 1237 1325 1617
Size: 54 1183 88 292
Fragments arranged by size:
1183 292 88 54
PspOMI G'GGCC_C
Cuts at: 0 883 919 1154 1175 1364 1617
Size: 883 36 235 21 189 253
Fragments arranged by size:
883 253 235 189 36 21
PstI C_TGCA'G
Cuts at: 0 385 1091 1354 1373 1617
Size: 385 706 263 19 244
Fragments arranged by size:
706 385 263 244 19
PvuII CAG'CTG
Cuts at: 0 955 1617
Size: 955 662
SapI GCTCTTCn'nnn_
Cuts at: 0 47 964 1617
Size: 47 917 653
Fragments arranged by size:
917 653 47
SbfI CC_TGCA'GG
Cuts at: 0 1354 1373 1617
Size: 1354 19 244
Fragments arranged by size:
1354 244 19
SfcI C'TryA_G
Cuts at: 0 381 567 1087 1350 1369 1617
Size: 381 186 520 263 19 248
Fragments arranged by size:
520 381 263 248 186 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 668 1617
Size: 668 949
SfoI GGC'GCC
Cuts at: 0 950 1617
Size: 950 667
SgrAI Cr'CCGG_yG
Cuts at: 0 1066 1617
Size: 1066 551
SmaI CCC'GGG
Cuts at: 0 882 1014 1112 1153 1159 1211 1310 1617
Size: 882 132 98 41 6 52 99 307
Fragments arranged by size:
882 307 132 99 98 52 41 6
SmlI C'TyrA_G
Cuts at: 0 90 1617
Size: 90 1527
SrfI GCCC'GGGC
Cuts at: 0 1159 1310 1617
Size: 1159 151 307
Fragments arranged by size:
1159 307 151
StuI AGG'CCT
Cuts at: 0 601 671 1617
Size: 601 70 946
Fragments arranged by size:
946 601 70
StyI C'CwwG_G
Cuts at: 0 176 851 1232 1597 1617
Size: 176 675 381 365 20
Fragments arranged by size:
675 381 365 176 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 118 1119 1617
Size: 118 1001 498
Fragments arranged by size:
1001 498 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 1617
Size: 64 32 1521
Fragments arranged by size:
1521 64 32
Tth111I GACn'n_nGTC
Cuts at: 0 1530 1617
Size: 1530 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 508 1617
Size: 508 1109
XmaI C'CCGG_G
Cuts at: 0 880 1012 1110 1151 1157 1209 1308 1617
Size: 880 132 98 41 6 52 99 309
Fragments arranged by size:
880 309 132 99 98 52 41 6
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI
AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI
BfrBI BglI BglII Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstEII
BstXI BstYI Bsu36I BtgI EaeI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PasI PfoI
PmlI PpuMI PspOMI PstI PvuII SapI SbfI SfcI
SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BbvCI BcgI
BclI BlpI BmgBI BmtI Bpu10I BsgI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI PacI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SgfI SnaBI SpeI SphI
SspI SwaI TatI XbaI XhoI XmnI ZraI