Restriction map for
pF1KA0003
(Linear) (Six-base) MAPSORT of: KIAA0003.seq Check: 5793 from: 1 to: 1494
KIAA0003.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1494
Size: 724 770
AflIII A'CryG_T
Cuts at: 0 216 261 1494
Size: 216 45 1233
Fragments arranged by size:
1233 216 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 224 1494
Size: 224 1270
AlwNI CAG_nnn'CTG
Cuts at: 0 288 489 696 753 1494
Size: 288 201 207 57 741
Fragments arranged by size:
741 288 207 201 57
ApaI G_GGCC'C
Cuts at: 0 1445 1494
Size: 1445 49
ApoI r'AATT_y
Cuts at: 0 495 1494
Size: 495 999
AseI AT'TA_AT
Cuts at: 0 911 1494
Size: 911 583
BanII G_rGCy'C
Cuts at: 0 1445 1494
Size: 1445 49
Bme1580I G_kGCm'C
Cuts at: 0 1320 1445 1494
Size: 1320 125 49
Fragments arranged by size:
1320 125 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 1146 1235 1439 1494
Size: 1146 89 204 55
Fragments arranged by size:
1146 204 89 55
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 227 1494
Size: 227 1267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 511 775 1494
Size: 403 108 264 719
Fragments arranged by size:
719 403 264 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 496 1494
Size: 319 177 998
Fragments arranged by size:
998 319 177
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 1494
Size: 439 1055
BsaAI yAC'GTr
Cuts at: 0 219 1494
Size: 219 1275
BsaBI GATnn'nnATC
Cuts at: 0 866 1494
Size: 866 628
BsaWI w'CCGG_w
Cuts at: 0 101 225 1054 1494
Size: 101 124 829 440
Fragments arranged by size:
829 440 124 101
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 1494
Size: 391 1103
BseYI C'CCAG_C
Cuts at: 0 1068 1494
Size: 1068 426
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 878 1494
Size: 75 803 616
Fragments arranged by size:
803 616 75
BsmI GAATG_Cn'
Cuts at: 0 357 1494
Size: 357 1137
Bsp1286I G_dGCh'C
Cuts at: 0 1320 1445 1494
Size: 1320 125 49
Fragments arranged by size:
1320 125 49
BsrDI GCAATG_nn'
Cuts at: 0 124 1494
Size: 124 1370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1113 1494
Size: 1113 381
BstYI r'GATC_y
Cuts at: 0 557 1494
Size: 557 937
BtgI C'CryG_G
Cuts at: 0 1401 1494
Size: 1401 93
DraI TTT'AAA
Cuts at: 0 1214 1494
Size: 1214 280
EarI CTCTTCn'nnn_
Cuts at: 0 240 614 635 830 1494
Size: 240 374 21 195 664
Fragments arranged by size:
664 374 240 195 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 511 724 775 1494
Size: 403 108 213 51 719
Fragments arranged by size:
719 403 213 108 51
EcoO109I rG'GnC_Cy
Cuts at: 0 1441 1494
Size: 1441 53
EcoRI G'AATT_C
Cuts at: 0 495 1494
Size: 495 999
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 670 1494
Size: 670 824
HaeII r_GCGC'y
Cuts at: 0 69 1494
Size: 69 1425
HincII GTy'rAC
Cuts at: 0 1328 1494
Size: 1328 166
HpaI GTT'AAC
Cuts at: 0 1328 1494
Size: 1328 166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 605 949 1494
Size: 308 297 344 545
Fragments arranged by size:
545 344 308 297
MslI CAynn'nnrTG
Cuts at: 0 1468 1494
Size: 1468 26
MspA1I CmG'CkG
Cuts at: 0 486 1494
Size: 486 1008
NcoI C'CATG_G
Cuts at: 0 1401 1494
Size: 1401 93
NspI r_CATG'y
Cuts at: 0 265 1116 1494
Size: 265 851 378
Fragments arranged by size:
851 378 265
PciI A'CATG_T
Cuts at: 0 261 1494
Size: 261 1233
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 1494
Size: 696 798
PfoI T'CCnGG_A
Cuts at: 0 689 1494
Size: 689 805
PmlI CAC'GTG
Cuts at: 0 219 1494
Size: 219 1275
PspOMI G'GGCC_C
Cuts at: 0 1441 1494
Size: 1441 53
PstI C_TGCA'G
Cuts at: 0 203 417 1494
Size: 203 214 1077
Fragments arranged by size:
1077 214 203
PvuII CAG'CTG
Cuts at: 0 486 1494
Size: 486 1008
SfcI C'TryA_G
Cuts at: 0 199 413 1200 1494
Size: 199 214 787 294
Fragments arranged by size:
787 294 214 199
SmlI C'TyrA_G
Cuts at: 0 298 475 1494
Size: 298 177 1019
Fragments arranged by size:
1019 298 177
SspI AAT'ATT
Cuts at: 0 1063 1494
Size: 1063 431
StyI C'CwwG_G
Cuts at: 0 1401 1494
Size: 1401 93
TatI w'GTAC_w
Cuts at: 0 182 1109 1494
Size: 182 927 385
Fragments arranged by size:
927 385 182
Tth111I GACn'n_nGTC
Cuts at: 0 769 1494
Size: 769 725
XbaI T'CTAG_A
Cuts at: 0 1002 1494
Size: 1002 492
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI AseI BanII
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaWI BseRI BseYI BsgI BsmI Bsp1286I BsrDI BstAPI
BstYI BtgI DraI EarI Eco57MI EcoO109I EcoRI FalI
HaeII HincII HpaI MmeI MslI MspA1I NcoI NspI
PciI PflMI PfoI PmlI PspOMI PstI PvuII SfcI
SmlI SspI StyI TatI Tth111I XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I
BsaHI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoNI EcoRV FseI
FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI XcmI XhoI XmaI XmnI ZraI