Restriction map for
pF1KA0008
(Linear) (Six-base) MAPSORT of: KIAA0008.seq Check: 8015 from: 1 to: 2538
KIAA0008.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 24 2538
Size: 24 2514
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1910 2538
Size: 979 931 628
Fragments arranged by size:
979 931 628
AflIII A'CryG_T
Cuts at: 0 1176 2022 2538
Size: 1176 846 516
Fragments arranged by size:
1176 846 516
AhdI GACnn_n'nnGTC
Cuts at: 0 1879 2538
Size: 1879 659
AleI CACnn'nnGTG
Cuts at: 0 1283 2538
Size: 1283 1255
AlwNI CAG_nnn'CTG
Cuts at: 0 948 1321 1792 2538
Size: 948 373 471 746
Fragments arranged by size:
948 746 471 373
ApoI r'AATT_y
Cuts at: 0 173 813 912 1073 1209 1567 2231 2262
Size: 173 640 99 161 136 358 664 31
Cuts at: 2262 2354 2533 2538
Size: 92 179 5
Fragments arranged by size:
664 640 358 179 173 161 136 99
92 31 5
AseI AT'TA_AT
Cuts at: 0 2210 2538
Size: 2210 328
BanI G'GyrC_C
Cuts at: 0 723 2538
Size: 723 1815
BbsI GAAGACnn'nnnn_
Cuts at: 0 744 967 2038 2538
Size: 744 223 1071 500
Fragments arranged by size:
1071 744 500 223
BclI T'GATC_A
Cuts at: 0 261 1580 2538
Size: 261 1319 958
Fragments arranged by size:
1319 958 261
BglII A'GATC_T
Cuts at: 0 1392 1512 2538
Size: 1392 120 1026
Fragments arranged by size:
1392 1026 120
BlpI GC'TnA_GC
Cuts at: 0 419 1271 2538
Size: 419 852 1267
Fragments arranged by size:
1267 852 419
BmgBI CAC'GTC
Cuts at: 0 602 2538
Size: 602 1936
BmrI ACTGGGnnnn_n'
Cuts at: 0 1010 2538
Size: 1010 1528
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 632 2195 2538
Size: 632 1563 343
Fragments arranged by size:
1563 632 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 2473 2538
Size: 1294 1179 65
Fragments arranged by size:
1294 1179 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 614 1043 1609 2538
Size: 185 429 429 566 929
Fragments arranged by size:
929 566 429 429 185
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 263 293 1014 1044 2538
Size: 263 30 721 30 1494
Fragments arranged by size:
1494 721 263 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2472 2538
Size: 2472 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 2538
Size: 604 1934
BsmI GAATG_Cn'
Cuts at: 0 411 1964 2404 2538
Size: 411 1553 440 134
Fragments arranged by size:
1553 440 411 134
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 633 750 2538
Size: 633 117 1788
Fragments arranged by size:
1788 633 117
BsrDI GCAATG_nn'
Cuts at: 0 248 1338 1751 2538
Size: 248 1090 413 787
Fragments arranged by size:
1090 787 413 248
BsrFI r'CCGG_y
Cuts at: 0 1996 2538
Size: 1996 542
BsrGI T'GTAC_A
Cuts at: 0 1507 2538
Size: 1507 1031
BstEII G'GTnAC_C
Cuts at: 0 2497 2538
Size: 2497 41
BstYI r'GATC_y
Cuts at: 0 1392 1512 2538
Size: 1392 120 1026
Fragments arranged by size:
1392 1026 120
Bsu36I CC'TnA_GG
Cuts at: 0 926 2538
Size: 926 1612
BtgI C'CryG_G
Cuts at: 0 1281 2538
Size: 1281 1257
DraI TTT'AAA
Cuts at: 0 354 1037 1184 1214 2538
Size: 354 683 147 30 1324
Fragments arranged by size:
1324 683 354 147 30
EarI CTCTTCn'nnn_
Cuts at: 0 1890 2186 2538
Size: 1890 296 352
Fragments arranged by size:
1890 352 296
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1294 1910 2473 2538
Size: 979 315 616 563 65
Fragments arranged by size:
979 616 563 315 65
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1432 2538
Size: 1432 1106
EcoO109I rG'GnC_Cy
Cuts at: 0 1150 1894 2538
Size: 1150 744 644
Fragments arranged by size:
1150 744 644
EcoRI G'AATT_C
Cuts at: 0 173 813 912 2262 2538
Size: 173 640 99 1350 276
Fragments arranged by size:
1350 640 276 173 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 560 933 2154 2538
Size: 560 373 1221 384
Fragments arranged by size:
1221 560 384 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 1865 1897 2140 2172 2448 2480
Size: 181 32 1652 32 243 32 276 32
Cuts at: 2480 2538
Size: 58
Fragments arranged by size:
1652 276 243 181 58 32 32 32
32
HincII GTy'rAC
Cuts at: 0 25 1929 2538
Size: 25 1904 609
Fragments arranged by size:
1904 609 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 549 2538
Size: 181 368 1989
Fragments arranged by size:
1989 368 181
MslI CAynn'nnrTG
Cuts at: 0 1086 1283 2538
Size: 1086 197 1255
Fragments arranged by size:
1255 1086 197
MspA1I CmG'CkG
Cuts at: 0 2451 2538
Size: 2451 87
NcoI C'CATG_G
Cuts at: 0 1281 2538
Size: 1281 1257
NspI r_CATG'y
Cuts at: 0 1180 2026 2476 2538
Size: 1180 846 450 62
Fragments arranged by size:
1180 846 450 62
PciI A'CATG_T
Cuts at: 0 1176 2022 2538
Size: 1176 846 516
Fragments arranged by size:
1176 846 516
PflMI CCAn_nnn'nTGG
Cuts at: 0 952 2538
Size: 952 1586
PfoI T'CCnGG_A
Cuts at: 0 2088 2538
Size: 2088 450
PpuMI rG'GwC_Cy
Cuts at: 0 1150 2538
Size: 1150 1388
PstI C_TGCA'G
Cuts at: 0 2434 2538
Size: 2434 104
PvuII CAG'CTG
Cuts at: 0 2451 2538
Size: 2451 87
SalI G'TCGA_C
Cuts at: 0 23 2538
Size: 23 2515
ScaI AGT'ACT
Cuts at: 0 45 2538
Size: 45 2493
SexAI A'CCwGG_T
Cuts at: 0 528 2418 2538
Size: 528 1890 120
Fragments arranged by size:
1890 528 120
SfcI C'TryA_G
Cuts at: 0 2430 2538
Size: 2430 108
SmlI C'TyrA_G
Cuts at: 0 200 629 1058 1588 2538
Size: 200 429 429 530 950
Fragments arranged by size:
950 530 429 429 200
SspI AAT'ATT
Cuts at: 0 350 2538
Size: 350 2188
StyI C'CwwG_G
Cuts at: 0 161 235 1281 2538
Size: 161 74 1046 1257
Fragments arranged by size:
1257 1046 161 74
SwaI ATTT'AAAT
Cuts at: 0 1214 2538
Size: 1214 1324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1029 1589 2538
Size: 1029 560 949
Fragments arranged by size:
1029 949 560
TatI w'GTAC_w
Cuts at: 0 43 1507 2538
Size: 43 1464 1031
Fragments arranged by size:
1464 1031 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1014 1046 1585 1617 2538
Size: 1014 32 539 32 921
Fragments arranged by size:
1014 921 539 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 530 2538
Size: 530 2008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 2538
Size: 477 2061
XmnI GAAnn'nnTTC
Cuts at: 0 916 2538
Size: 916 1622
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlwNI ApoI AseI
BanI BbsI BclI BglII BlpI BmgBI BmrI BplI
BpmI BpuEI BsaXI BseRI BsgI BsmI BspMI BsrDI
BsrFI BsrGI BstEII BstYI Bsu36I BtgI DraI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII MmeI
MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI
PstI PvuII SalI ScaI SexAI SfcI SmlI SspI
StyI SwaI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanII BbeI BbvCI BcgI BciVI BfrBI BglI
Bme1580I BmtI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BsrBI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoRV FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
XbaI XhoI XmaI ZraI