Restriction map for
pF1KA0011
(Linear) (Six-base) MAPSORT of: KIAA0011.seq Check: 2181 from: 1 to: 2733
KIAA0011.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1614 2733
Size: 1614 1119
AatII G_ACGT'C
Cuts at: 0 1935 2733
Size: 1935 798
AccI GT'mk_AC
Cuts at: 0 68 1536 2733
Size: 68 1468 1197
Fragments arranged by size:
1468 1197 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 239 481 568 617 782 833 1118
Size: 101 138 242 87 49 165 51 285
Cuts at: 1118 2318 2733
Size: 1200 415
Fragments arranged by size:
1200 415 285 242 165 138 101 87
51 49
AfeI AGC'GCT
Cuts at: 0 1156 1963 2733
Size: 1156 807 770
Fragments arranged by size:
1156 807 770
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1405 1437 2733
Size: 1405 32 1296
Fragments arranged by size:
1405 1296 32
AlwNI CAG_nnn'CTG
Cuts at: 0 591 2733
Size: 591 2142
ApaI G_GGCC'C
Cuts at: 0 646 787 1371 2733
Size: 646 141 584 1362
Fragments arranged by size:
1362 646 584 141
ApoI r'AATT_y
Cuts at: 0 1915 2733
Size: 1915 818
AvrII C'CTAG_G
Cuts at: 0 280 2733
Size: 280 2453
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 562 595 2733
Size: 562 33 2138
Fragments arranged by size:
2138 562 33
BamHI G'GATC_C
Cuts at: 0 2137 2733
Size: 2137 596
BanI G'GyrC_C
Cuts at: 0 1105 1205 1453 2116 2733
Size: 1105 100 248 663 617
Fragments arranged by size:
1105 663 617 248 100
BanII G_rGCy'C
Cuts at: 0 605 646 787 799 1197 1371 2186 2649
Size: 605 41 141 12 398 174 815 463
Cuts at: 2649 2733
Size: 84
Fragments arranged by size:
815 605 463 398 174 141 84 41
12
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 2733
Size: 743 1990
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1214 1248 2733
Size: 1214 34 1485
Fragments arranged by size:
1485 1214 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1772 2733
Size: 1772 961
BclI T'GATC_A
Cuts at: 0 192 2733
Size: 192 2541
BglI GCCn_nnn'nGGC
Cuts at: 0 568 1558 1695 2733
Size: 568 990 137 1038
Fragments arranged by size:
1038 990 568 137
BglII A'GATC_T
Cuts at: 0 246 2271 2733
Size: 246 2025 462
Fragments arranged by size:
2025 462 246
BlpI GC'TnA_GC
Cuts at: 0 1562 2733
Size: 1562 1171
Bme1580I G_kGCm'C
Cuts at: 0 646 787 824 1210 1371 2733
Size: 646 141 37 386 161 1362
Fragments arranged by size:
1362 646 386 161 141 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 981 1204 1424 2626 2733
Size: 981 223 220 1202 107
Fragments arranged by size:
1202 981 223 220 107
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 224 2733
Size: 224 2509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 213 733 1239 1300 2521 2733
Size: 194 19 520 506 61 1221 212
Fragments arranged by size:
1221 520 506 212 194 61 19
Bpu10I CC'TnA_GC
Cuts at: 0 809 1672 2018 2733
Size: 809 863 346 715
Fragments arranged by size:
863 809 715 346
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1048 1402 1989 2733
Size: 1048 354 587 744
Fragments arranged by size:
1048 744 587 354
BsaI GGTCTCn'nnnn_
Cuts at: 0 276 385 520 1495 2733
Size: 276 109 135 975 1238
Fragments arranged by size:
1238 975 276 135 109
BsaHI Gr'CG_yC
Cuts at: 0 1932 2733
Size: 1932 801
BsaWI w'CCGG_w
Cuts at: 0 1545 1904 2733
Size: 1545 359 829
Fragments arranged by size:
1545 829 359
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 698 728 1045 1075 2524 2554 2733
Size: 698 30 317 30 1449 30 179
Fragments arranged by size:
1449 698 317 179 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 213 1069 1460 2096 2733
Size: 213 856 391 636 637
Fragments arranged by size:
856 637 636 391 213
BseYI C'CCAG_C
Cuts at: 0 1157 2733
Size: 1157 1576
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 2733
Size: 152 2581
BsiHKAI G_wGCw'C
Cuts at: 0 605 726 799 2186 2733
Size: 605 121 73 1387 547
Fragments arranged by size:
1387 605 547 121 73
BsmI GAATG_Cn'
Cuts at: 0 2469 2733
Size: 2469 264
Bsp1286I G_dGCh'C
Cuts at: 0 605 646 726 787 799 824 1197 1210
Size: 605 41 80 61 12 25 373 13
Cuts at: 1210 1371 2186 2649 2733
Size: 161 815 463 84
Fragments arranged by size:
815 605 463 373 161 84 80 61
41 25 13 12
BspEI T'CCGG_A
Cuts at: 0 1545 2733
Size: 1545 1188
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 1052 1614 2733
Size: 16 1036 562 1119
Fragments arranged by size:
1119 1036 562 16
BsrBI CCG'CTC
Cuts at: 0 1036 1188 2733
Size: 1036 152 1545
Fragments arranged by size:
1545 1036 152
BsrDI GCAATG_nn'
Cuts at: 0 2186 2733
Size: 2186 547
BsrFI r'CCGG_y
Cuts at: 0 40 2733
Size: 40 2693
BsrGI T'GTAC_A
Cuts at: 0 1077 2733
Size: 1077 1656
BssSI C'ACGA_G
Cuts at: 0 836 2733
Size: 836 1897
BstBI TT'CG_AA
Cuts at: 0 2583 2733
Size: 2583 150
BstEII G'GTnAC_C
Cuts at: 0 2074 2733
Size: 2074 659
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1157 2733
Size: 1157 1576
BstYI r'GATC_y
Cuts at: 0 246 677 841 2137 2271 2733
Size: 246 431 164 1296 134 462
Fragments arranged by size:
1296 462 431 246 164 134
Bsu36I CC'TnA_GG
Cuts at: 0 515 638 2733
Size: 515 123 2095
Fragments arranged by size:
2095 515 123
BtgI C'CryG_G
Cuts at: 0 47 727 2598 2733
Size: 47 680 1871 135
Fragments arranged by size:
1871 680 135 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2698 2733
Size: 2698 35
BtsI GCAGTG_nn'
Cuts at: 0 867 1586 2099 2733
Size: 867 719 513 634
Fragments arranged by size:
867 719 634 513
EaeI y'GGCC_r
Cuts at: 0 297 484 2733
Size: 297 187 2249
Fragments arranged by size:
2249 297 187
EarI CTCTTCn'nnn_
Cuts at: 0 120 298 593 704 2733
Size: 120 178 295 111 2029
Fragments arranged by size:
2029 295 178 120 111
EcoICRI GAG'CTC
Cuts at: 0 603 797 2184 2733
Size: 603 194 1387 549
Fragments arranged by size:
1387 603 549 194
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 194 213 239 481 568 617 733
Size: 101 93 19 26 242 87 49 116
Cuts at: 733 782 833 1118 1239 1300 2318 2521 2733
Size: 49 51 285 121 61 1018 203 212
Fragments arranged by size:
1018 285 242 212 203 121 116 101
93 87 61 51 49 49 26 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 1048 2111 2733
Size: 34 1014 1063 622
Fragments arranged by size:
1063 1014 622 34
EcoO109I rG'GnC_Cy
Cuts at: 0 284 328 338 509 642 643 783 1303
Size: 284 44 10 171 133 1 140 520
Cuts at: 1303 1326 1334 1367 1413 1551 1697 1923 2331
Size: 23 8 33 46 138 146 226 408
Cuts at: 2331 2733
Size: 402
Fragments arranged by size:
520 408 402 284 226 171 146 140
138 133 46 44 33 23 10 8
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 1029 1983 2112 2733
Size: 589 440 954 129 621
Fragments arranged by size:
954 621 589 440 129
FseI GG_CCGG'CC
Cuts at: 0 44 2733
Size: 44 2689
FspI TGC'GCA
Cuts at: 0 2449 2733
Size: 2449 284
HaeII r_GCGC'y
Cuts at: 0 1158 1965 2733
Size: 1158 807 768
Fragments arranged by size:
1158 807 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1045 1077 2268 2300 2733
Size: 784 32 229 32 1191 32 433
Fragments arranged by size:
1191 784 433 229 32 32 32
HincII GTy'rAC
Cuts at: 0 2373 2733
Size: 2373 360
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 2165 2691 2728 2733
Size: 419 1746 526 37 5
Fragments arranged by size:
1746 526 419 37 5
MslI CAynn'nnrTG
Cuts at: 0 61 412 2654 2733
Size: 61 351 2242 79
Fragments arranged by size:
2242 351 79 61
MspA1I CmG'CkG
Cuts at: 0 1776 2499 2733
Size: 1776 723 234
Fragments arranged by size:
1776 723 234
NaeI GCC'GGC
Cuts at: 0 42 2733
Size: 42 2691
NgoMIV G'CCGG_C
Cuts at: 0 40 2733
Size: 40 2693
NspI r_CATG'y
Cuts at: 0 2454 2733
Size: 2454 279
PasI CC'CwG_GG
Cuts at: 0 30 2733
Size: 30 2703
PfoI T'CCnGG_A
Cuts at: 0 75 2733
Size: 75 2658
PpuMI rG'GwC_Cy
Cuts at: 0 1303 1334 1413 1923 2331 2733
Size: 1303 31 79 510 408 402
Fragments arranged by size:
1303 510 408 402 79 31
PsiI TTA'TAA
Cuts at: 0 1727 2733
Size: 1727 1006
PspOMI G'GGCC_C
Cuts at: 0 642 783 1367 2733
Size: 642 141 584 1366
Fragments arranged by size:
1366 642 584 141
PstI C_TGCA'G
Cuts at: 0 1625 1775 2166 2498 2733
Size: 1625 150 391 332 235
Fragments arranged by size:
1625 391 332 235 150
PvuII CAG'CTG
Cuts at: 0 2499 2733
Size: 2499 234
SacI G_AGCT'C
Cuts at: 0 605 799 2186 2733
Size: 605 194 1387 547
Fragments arranged by size:
1387 605 547 194
SanDI GG'GwC_CC
Cuts at: 0 2331 2733
Size: 2331 402
SapI GCTCTTCn'nnn_
Cuts at: 0 593 2733
Size: 593 2140
ScaI AGT'ACT
Cuts at: 0 1523 2733
Size: 1523 1210
SfcI C'TryA_G
Cuts at: 0 815 1621 1643 1771 2162 2240 2494 2733
Size: 815 806 22 128 391 78 254 239
Fragments arranged by size:
815 806 391 254 239 128 78 22
SmlI C'TyrA_G
Cuts at: 0 1027 1417 1968 2733
Size: 1027 390 551 765
Fragments arranged by size:
1027 765 551 390
SphI G_CATG'C
Cuts at: 0 2454 2733
Size: 2454 279
StuI AGG'CCT
Cuts at: 0 2092 2733
Size: 2092 641
StyI C'CwwG_G
Cuts at: 0 280 440 647 941 1329 2079 2345 2733
Size: 280 160 207 294 388 750 266 388
Fragments arranged by size:
750 388 388 294 280 266 207 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1295 2733
Size: 1295 1438
TatI w'GTAC_w
Cuts at: 0 1077 1521 1972 2733
Size: 1077 444 451 761
Fragments arranged by size:
1077 761 451 444
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 294 326 698 730 1131 1163
Size: 71 32 191 32 372 32 401 32
Cuts at: 1163 1377 1409 2733
Size: 214 32 1324
Fragments arranged by size:
1324 401 372 214 191 71 32 32
32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1378 2596 2733
Size: 1378 1218 137
Fragments arranged by size:
1378 1218 137
ZraI GAC'GTC
Cuts at: 0 1933 2733
Size: 1933 800
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AloI AlwNI ApaI
ApoI AvrII BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII
MmeI MslI MspA1I NaeI NgoMIV NspI PasI PfoI
PpuMI PsiI PspOMI PstI PvuII SacI SanDI SapI
ScaI SfcI SmlI SphI StuI StyI TaqII TatI
TstI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI AvaI BbeI BbvCI BfrBI
BmgBI BmtI BsaAI BsaBI BsiEI BsiWI BsmBI BspHI
BssHII BstAPI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoRI EcoRV FspAI HindIII HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
XmnI