Restriction map for
pF1KA0012
(Linear) (Six-base) MAPSORT of: KIAA0012.seq Check: 9434 from: 1 to: 2358
KIAA0012.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2062 2358
Size: 2062 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 86 849 2358
Size: 28 58 763 1509
Fragments arranged by size:
1509 763 58 28
AflII C'TTAA_G
Cuts at: 0 2313 2358
Size: 2313 45
AflIII A'CryG_T
Cuts at: 0 1712 2358
Size: 1712 646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 517 2358
Size: 517 1841
AlwNI CAG_nnn'CTG
Cuts at: 0 1000 2358
Size: 1000 1358
ApoI r'AATT_y
Cuts at: 0 76 268 424 601 774 987 1221 1600
Size: 76 192 156 177 173 213 234 379
Cuts at: 1600 2358
Size: 758
Fragments arranged by size:
758 379 234 213 192 177 173 156
76
AseI AT'TA_AT
Cuts at: 0 2327 2358
Size: 2327 31
BamHI G'GATC_C
Cuts at: 0 788 2358
Size: 788 1570
BanI G'GyrC_C
Cuts at: 0 1106 2117 2358
Size: 1106 1011 241
Fragments arranged by size:
1106 1011 241
BbsI GAAGACnn'nnnn_
Cuts at: 0 542 1283 2358
Size: 542 741 1075
Fragments arranged by size:
1075 741 542
BclI T'GATC_A
Cuts at: 0 1484 1491 2358
Size: 1484 7 867
Fragments arranged by size:
1484 867 7
BfrBI ATG'CAT
Cuts at: 0 373 1915 2358
Size: 373 1542 443
Fragments arranged by size:
1542 443 373
Bme1580I G_kGCm'C
Cuts at: 0 1109 1937 2358
Size: 1109 828 421
Fragments arranged by size:
1109 828 421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 778 1888 2358
Size: 778 1110 470
Fragments arranged by size:
1110 778 470
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 2358
Size: 331 2027
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 2358
Size: 109 2249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1589 1619 2358
Size: 1589 30 739
Fragments arranged by size:
1589 739 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 2358
Size: 559 1799
BsiHKAI G_wGCw'C
Cuts at: 0 442 2358
Size: 442 1916
BsmI GAATG_Cn'
Cuts at: 0 1376 2358
Size: 1376 982
Bsp1286I G_dGCh'C
Cuts at: 0 442 1109 1937 2358
Size: 442 667 828 421
Fragments arranged by size:
828 667 442 421
BspHI T'CATG_A
Cuts at: 0 2004 2157 2358
Size: 2004 153 201
Fragments arranged by size:
2004 201 153
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2062 2358
Size: 2062 296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 2358
Size: 379 1979
BstBI TT'CG_AA
Cuts at: 0 970 2358
Size: 970 1388
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1770 2358
Size: 1770 588
BstYI r'GATC_y
Cuts at: 0 788 1812 2164 2358
Size: 788 1024 352 194
Fragments arranged by size:
1024 788 352 194
Bsu36I CC'TnA_GG
Cuts at: 0 542 2358
Size: 542 1816
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 941 2358
Size: 941 1417
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1006 1041 2358
Size: 1006 35 1317
Fragments arranged by size:
1317 1006 35
DraI TTT'AAA
Cuts at: 0 1283 2358
Size: 1283 1075
DraIII CAC_nnn'GTG
Cuts at: 0 582 2358
Size: 582 1776
EaeI y'GGCC_r
Cuts at: 0 2249 2358
Size: 2249 109
EarI CTCTTCn'nnn_
Cuts at: 0 2059 2358
Size: 2059 299
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 86 778 849 1888 2358
Size: 28 58 692 71 1039 470
Fragments arranged by size:
1039 692 470 71 58 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1699 2358
Size: 1699 659
EcoO109I rG'GnC_Cy
Cuts at: 0 546 2358
Size: 546 1812
EcoRI G'AATT_C
Cuts at: 0 601 774 1221 2358
Size: 601 173 447 1137
Fragments arranged by size:
1137 601 447 173
EcoRV GAT'ATC
Cuts at: 0 255 2358
Size: 255 2103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2097 2358
Size: 2097 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 245 1179 1211 1476 1508 1589
Size: 181 32 32 934 32 265 32 81
Cuts at: 1589 1609 1621 1641 2358
Size: 20 12 20 717
Fragments arranged by size:
934 717 265 181 81 32 32 32
32 20 20 12
HindIII A'AGCT_T
Cuts at: 0 1403 2358
Size: 1403 955
MfeI C'AATT_G
Cuts at: 0 473 1204 2358
Size: 473 731 1154
Fragments arranged by size:
1154 731 473
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1100 2358
Size: 1100 1258
MscI TGG'CCA
Cuts at: 0 2251 2358
Size: 2251 107
MslI CAynn'nnrTG
Cuts at: 0 1578 1771 2358
Size: 1578 193 587
Fragments arranged by size:
1578 587 193
MspA1I CmG'CkG
Cuts at: 0 798 858 2358
Size: 798 60 1500
Fragments arranged by size:
1500 798 60
NsiI A_TGCA'T
Cuts at: 0 375 1917 2358
Size: 375 1542 441
Fragments arranged by size:
1542 441 375
NspI r_CATG'y
Cuts at: 0 1023 1716 2358
Size: 1023 693 642
Fragments arranged by size:
1023 693 642
PciI A'CATG_T
Cuts at: 0 1712 2358
Size: 1712 646
PflMI CCAn_nnn'nTGG
Cuts at: 0 282 2358
Size: 282 2076
PfoI T'CCnGG_A
Cuts at: 0 1449 2358
Size: 1449 909
PsiI TTA'TAA
Cuts at: 0 1661 2358
Size: 1661 697
PstI C_TGCA'G
Cuts at: 0 1804 2358
Size: 1804 554
PvuII CAG'CTG
Cuts at: 0 798 858 2358
Size: 798 60 1500
Fragments arranged by size:
1500 798 60
ScaI AGT'ACT
Cuts at: 0 2209 2358
Size: 2209 149
SfcI C'TryA_G
Cuts at: 0 635 847 1800 2358
Size: 635 212 953 558
Fragments arranged by size:
953 635 558 212
SmlI C'TyrA_G
Cuts at: 0 346 2313 2358
Size: 346 1967 45
Fragments arranged by size:
1967 346 45
SspI AAT'ATT
Cuts at: 0 2331 2358
Size: 2331 27
StuI AGG'CCT
Cuts at: 0 901 2358
Size: 901 1457
StyI C'CwwG_G
Cuts at: 0 2276 2358
Size: 2276 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1526 1938 2358
Size: 1526 412 420
Fragments arranged by size:
1526 420 412
TatI w'GTAC_w
Cuts at: 0 2207 2358
Size: 2207 151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 998 1030 2358
Size: 998 32 1328
Fragments arranged by size:
1328 998 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1773 2358
Size: 1773 585
XmnI GAAnn'nnTTC
Cuts at: 0 778 2198 2358
Size: 778 1420 160
Fragments arranged by size:
1420 778 160
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AlwNI ApoI AseI
BamHI BanI BbsI BclI BfrBI Bme1580I BpmI BpuEI
BsaI BsaXI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI CspCI DraI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI Hin4I HindIII MfeI MmeI MscI MslI MspA1I
NsiI NspI PciI PflMI PfoI PsiI PstI PvuII
ScaI SfcI SmlI SspI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BanII BbeI BbvCI BcgI BciVI BglI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstEII BstZ17I BtgI
BtsI ClaI DrdI EagI EciI EcoICRI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI
ZraI