(Linear) (Six-base) MAPSORT of: KIAA0016.seq Check: 5363 from: 1 to: 435 KIAA0016.orf With 182 enzymes: SgfI * October 24, 2006 15:10 .. AccI GT'mk_AC Cuts at: 0 269 435 Size: 269 166 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 435 Size: 209 226 AlwNI CAG_nnn'CTG Cuts at: 0 318 435 Size: 318 117 ApaI G_GGCC'C Cuts at: 0 45 435 Size: 45 390 BanII G_rGCy'C Cuts at: 0 45 435 Size: 45 390 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 51 85 435 Size: 51 34 350 Fragments arranged by size: 350 51 34 Bme1580I G_kGCm'C Cuts at: 0 45 435 Size: 45 390 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 230 435 Size: 230 205 BseYI C'CCAG_C Cuts at: 0 154 435 Size: 154 281 BsiEI CG_ry'CG Cuts at: 0 75 435 Size: 75 360 BsiHKAI G_wGCw'C Cuts at: 0 408 435 Size: 408 27 Bsp1286I G_dGCh'C Cuts at: 0 45 408 435 Size: 45 363 27 Fragments arranged by size: 363 45 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 312 435 Size: 312 123 BsrFI r'CCGG_y Cuts at: 0 28 435 Size: 28 407 BstAPI GCAn_nnn'nTGC Cuts at: 0 318 435 Size: 318 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 6 435 Size: 6 429 EarI CTCTTCn'nnn_ Cuts at: 0 227 344 435 Size: 227 117 91 Fragments arranged by size: 227 117 91 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 209 435 Size: 209 226 EcoO109I rG'GnC_Cy Cuts at: 0 41 42 435 Size: 41 1 393 Fragments arranged by size: 393 41 1 HaeII r_GCGC'y Cuts at: 0 20 435 Size: 20 415 HindIII A'AGCT_T Cuts at: 0 136 435 Size: 136 299 MfeI C'AATT_G Cuts at: 0 287 435 Size: 287 148 PspOMI G'GGCC_C Cuts at: 0 41 435 Size: 41 394 PstI C_TGCA'G Cuts at: 0 323 435 Size: 323 112 SfcI C'TryA_G Cuts at: 0 319 435 Size: 319 116 SgrAI Cr'CCGG_yG Cuts at: 0 28 435 Size: 28 407 SmlI C'TyrA_G Cuts at: 0 245 435 Size: 245 190 StyI C'CwwG_G Cuts at: 0 143 435 Size: 143 292 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 338 370 435 Size: 338 32 65 Fragments arranged by size: 338 65 32 XmnI GAAnn'nnTTC Cuts at: 0 194 205 435 Size: 194 11 230 Fragments arranged by size: 230 194 11 Enzymes that do cut: AccI AcuI AlwNI ApaI BanII BcgI Bme1580I BpuEI BseYI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BstAPI BtgZI EarI Eco57MI EcoO109I HaeII HindIII MfeI PspOMI PstI SfcI SgrAI SmlI StyI TstI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI