Restriction map for
pF1KSDA0017
(Linear) (Six-base) MAPSORT of: KIAA0017.seq Check: 7352 from: 1 to: 3651
KIAA0017.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3449 3651
Size: 3449 202
AccI GT'mk_AC
Cuts at: 0 1389 3651
Size: 1389 2262
Acc65I G'GTAC_C
Cuts at: 0 2075 3651
Size: 2075 1576
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1694 3602 3651
Size: 1694 1908 49
Fragments arranged by size:
1908 1694 49
AhdI GACnn_n'nnGTC
Cuts at: 0 1385 3651
Size: 1385 2266
AleI CACnn'nnGTG
Cuts at: 0 496 1447 3651
Size: 496 951 2204
Fragments arranged by size:
2204 951 496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3403 3651
Size: 3403 248
AlwNI CAG_nnn'CTG
Cuts at: 0 715 3651
Size: 715 2936
ApoI r'AATT_y
Cuts at: 0 1054 2218 2359 3651
Size: 1054 1164 141 1292
Fragments arranged by size:
1292 1164 1054 141
AvaI C'yCGr_G
Cuts at: 0 2251 3622 3651
Size: 2251 1371 29
Fragments arranged by size:
2251 1371 29
BamHI G'GATC_C
Cuts at: 0 369 1704 1857 2975 3204 3651
Size: 369 1335 153 1118 229 447
Fragments arranged by size:
1335 1118 447 369 229 153
BanI G'GyrC_C
Cuts at: 0 217 2075 3651
Size: 217 1858 1576
Fragments arranged by size:
1858 1576 217
BanII G_rGCy'C
Cuts at: 0 1975 2562 3651
Size: 1975 587 1089
Fragments arranged by size:
1975 1089 587
BbsI GAAGACnn'nnnn_
Cuts at: 0 1615 2310 2708 3322 3651
Size: 1615 695 398 614 329
Fragments arranged by size:
1615 695 614 398 329
BbvCI CC'TCA_GC
Cuts at: 0 2023 3481 3651
Size: 2023 1458 170
Fragments arranged by size:
2023 1458 170
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1786 1820 3651
Size: 1786 34 1831
Fragments arranged by size:
1831 1786 34
BclI T'GATC_A
Cuts at: 0 3272 3651
Size: 3272 379
BglII A'GATC_T
Cuts at: 0 107 3651
Size: 107 3544
BmgBI CAC'GTC
Cuts at: 0 3398 3651
Size: 3398 253
BmrI ACTGGGnnnn_n'
Cuts at: 0 2057 3651
Size: 2057 1594
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1989 3651
Size: 1989 1662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1811 2830 3353 3389 3651
Size: 737 1074 1019 523 36 262
Fragments arranged by size:
1074 1019 737 523 262 36
Bpu10I CC'TnA_GC
Cuts at: 0 2023 3481 3651
Size: 2023 1458 170
Fragments arranged by size:
2023 1458 170
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 3651
Size: 1345 2306
BsaI GGTCTCn'nnnn_
Cuts at: 0 1808 3521 3651
Size: 1808 1713 130
Fragments arranged by size:
1808 1713 130
BsaBI GATnn'nnATC
Cuts at: 0 3203 3651
Size: 3203 448
BsaWI w'CCGG_w
Cuts at: 0 185 3632 3651
Size: 185 3447 19
Fragments arranged by size:
3447 185 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 506 536 1454 1484 1673 1703 3651
Size: 506 30 918 30 189 30 1948
Fragments arranged by size:
1948 918 506 189 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1694 3651
Size: 1694 1957
BseYI C'CCAG_C
Cuts at: 0 611 1899 1904 1959 3133 3651
Size: 611 1288 5 55 1174 518
Fragments arranged by size:
1288 1174 611 518 55 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1570 1661 2700 3651
Size: 1570 91 1039 951
Fragments arranged by size:
1570 1039 951 91
BsiHKAI G_wGCw'C
Cuts at: 0 2562 3651
Size: 2562 1089
BsmI GAATG_Cn'
Cuts at: 0 1443 1568 2120 2521 3651
Size: 1443 125 552 401 1130
Fragments arranged by size:
1443 1130 552 401 125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3289 3582 3651
Size: 3289 293 69
Fragments arranged by size:
3289 293 69
Bsp1286I G_dGCh'C
Cuts at: 0 1975 2562 3651
Size: 1975 587 1089
Fragments arranged by size:
1975 1089 587
BspEI T'CCGG_A
Cuts at: 0 3632 3651
Size: 3632 19
BspHI T'CATG_A
Cuts at: 0 3275 3651
Size: 3275 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1542 2672 3449 3651
Size: 1542 1130 777 202
Fragments arranged by size:
1542 1130 777 202
BsrBI CCG'CTC
Cuts at: 0 2153 2493 3651
Size: 2153 340 1158
Fragments arranged by size:
2153 1158 340
BsrDI GCAATG_nn'
Cuts at: 0 2580 3651
Size: 2580 1071
BsrGI T'GTAC_A
Cuts at: 0 8 3651
Size: 8 3643
BstEII G'GTnAC_C
Cuts at: 0 784 1360 3651
Size: 784 576 2291
Fragments arranged by size:
2291 784 576
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1134 2986 3114 3651
Size: 1134 1852 128 537
Fragments arranged by size:
1852 1134 537 128
BstYI r'GATC_y
Cuts at: 0 107 369 971 1704 1857 2975 3204 3651
Size: 107 262 602 733 153 1118 229 447
Fragments arranged by size:
1118 733 602 447 262 229 153 107
BtgI C'CryG_G
Cuts at: 0 99 3234 3551 3651
Size: 99 3135 317 100
Fragments arranged by size:
3135 317 100 99
BtsI GCAGTG_nn'
Cuts at: 0 249 1649 2338 2587 3651
Size: 249 1400 689 249 1064
Fragments arranged by size:
1400 1064 689 249 249
ClaI AT'CG_AT
Cuts at: 0 889 3651
Size: 889 2762
DraI TTT'AAA
Cuts at: 0 650 3651
Size: 650 3001
DrdI GACnn_nn'nnGTC
Cuts at: 0 1594 1837 3303 3651
Size: 1594 243 1466 348
Fragments arranged by size:
1594 1466 348 243
EaeI y'GGCC_r
Cuts at: 0 351 1244 1775 2138 2264 2501 2726 3651
Size: 351 893 531 363 126 237 225 925
Fragments arranged by size:
925 893 531 363 351 237 225 126
EarI CTCTTCn'nnn_
Cuts at: 0 2107 2831 3651
Size: 2107 724 820
Fragments arranged by size:
2107 820 724
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 837 3651
Size: 837 2814
EcoICRI GAG'CTC
Cuts at: 0 2560 3651
Size: 2560 1091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 737 1694 1811 2830 3353 3389 3602 3651
Size: 737 957 117 1019 523 36 213 49
Fragments arranged by size:
1019 957 737 523 213 117 49 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 845 1281 3651
Size: 845 436 2370
Fragments arranged by size:
2370 845 436
EcoO109I rG'GnC_Cy
Cuts at: 0 1288 2084 2733 2841 3251 3651
Size: 1288 796 649 108 410 400
Fragments arranged by size:
1288 796 649 410 400 108
EcoRV GAT'ATC
Cuts at: 0 798 927 3630 3651
Size: 798 129 2703 21
Fragments arranged by size:
2703 798 129 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 506 538 731 763 1105 1137 1195 1224
Size: 506 32 193 32 342 32 58 29
Cuts at: 1224 1227 1256 1371 1403 1673 1705 1786 1818
Size: 3 29 115 32 270 32 81 32
Cuts at: 1818 3651
Size: 1833
Fragments arranged by size:
1833 506 342 270 193 115 81 58
32 32 32 32 32 32 29 29
3
HincII GTy'rAC
Cuts at: 0 1390 3651
Size: 1390 2261
HindIII A'AGCT_T
Cuts at: 0 2785 3651
Size: 2785 866
KpnI G_GTAC'C
Cuts at: 0 2079 3651
Size: 2079 1572
MfeI C'AATT_G
Cuts at: 0 1628 3651
Size: 1628 2023
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 492 504 610 1181 2714 3651
Size: 492 12 106 571 1533 937
Fragments arranged by size:
1533 937 571 492 106 12
MscI TGG'CCA
Cuts at: 0 353 1246 1777 2140 2266 2728 3651
Size: 353 893 531 363 126 462 923
Fragments arranged by size:
923 893 531 462 363 353 126
MslI CAynn'nnrTG
Cuts at: 0 496 1018 1423 1447 3160 3385 3651
Size: 496 522 405 24 1713 225 266
Fragments arranged by size:
1713 522 496 405 266 225 24
MspA1I CmG'CkG
Cuts at: 0 803 1719 1800 2643 3651
Size: 803 916 81 843 1008
Fragments arranged by size:
1008 916 843 803 81
NcoI C'CATG_G
Cuts at: 0 3551 3651
Size: 3551 100
NdeI CA'TA_TG
Cuts at: 0 3157 3651
Size: 3157 494
NspI r_CATG'y
Cuts at: 0 1890 3430 3651
Size: 1890 1540 221
Fragments arranged by size:
1890 1540 221
PflMI CCAn_nnn'nTGG
Cuts at: 0 3429 3651
Size: 3429 222
PfoI T'CCnGG_A
Cuts at: 0 714 3113 3651
Size: 714 2399 538
Fragments arranged by size:
2399 714 538
PpuMI rG'GwC_Cy
Cuts at: 0 1288 2084 2733 2841 3651
Size: 1288 796 649 108 810
Fragments arranged by size:
1288 810 796 649 108
PshAI GACnn'nnGTC
Cuts at: 0 251 3651
Size: 251 3400
PspXI vC'TCGA_Gb
Cuts at: 0 3622 3651
Size: 3622 29
PstI C_TGCA'G
Cuts at: 0 447 2345 3651
Size: 447 1898 1306
Fragments arranged by size:
1898 1306 447
PvuII CAG'CTG
Cuts at: 0 1719 2643 3651
Size: 1719 924 1008
Fragments arranged by size:
1719 1008 924
SacI G_AGCT'C
Cuts at: 0 2562 3651
Size: 2562 1089
SalI G'TCGA_C
Cuts at: 0 1388 3651
Size: 1388 2263
SanDI GG'GwC_CC
Cuts at: 0 1288 2084 3651
Size: 1288 796 1567
Fragments arranged by size:
1567 1288 796
SapI GCTCTTCn'nnn_
Cuts at: 0 2107 3651
Size: 2107 1544
ScaI AGT'ACT
Cuts at: 0 743 3651
Size: 743 2908
SexAI A'CCwGG_T
Cuts at: 0 1356 3651
Size: 1356 2295
SfcI C'TryA_G
Cuts at: 0 443 1469 2005 2341 3101 3209 3651
Size: 443 1026 536 336 760 108 442
Fragments arranged by size:
1026 760 536 443 442 336 108
SmlI C'TyrA_G
Cuts at: 0 1324 3622 3651
Size: 1324 2298 29
Fragments arranged by size:
2298 1324 29
SphI G_CATG'C
Cuts at: 0 1890 3651
Size: 1890 1761
SspI AAT'ATT
Cuts at: 0 985 1998 3651
Size: 985 1013 1653
Fragments arranged by size:
1653 1013 985
StyI C'CwwG_G
Cuts at: 0 642 1544 2564 2729 3551 3651
Size: 642 902 1020 165 822 100
Fragments arranged by size:
1020 902 822 642 165 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1924 1951 2367 2710 3608 3651
Size: 1924 27 416 343 898 43
Fragments arranged by size:
1924 898 416 343 43 27
TatI w'GTAC_w
Cuts at: 0 8 147 741 1727 2345 3651
Size: 8 139 594 986 618 1306
Fragments arranged by size:
1306 986 618 594 139 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1313 1345 3651
Size: 1313 32 2306
Fragments arranged by size:
2306 1313 32
Tth111I GACn'n_nGTC
Cuts at: 0 1515 2041 2635 3651
Size: 1515 526 594 1016
Fragments arranged by size:
1515 1016 594 526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 3651
Size: 316 3335
XhoI C'TCGA_G
Cuts at: 0 3622 3651
Size: 3622 29
XmnI GAAnn'nnTTC
Cuts at: 0 63 2101 3651
Size: 63 2038 1550
Fragments arranged by size:
2038 1550 63
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlfI AlwNI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BclI BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrGI BstEII BstXI BstYI BtgI BtsI ClaI DraI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV Hin4I HincII HindIII KpnI MfeI MmeI MscI
MslI MspA1I NcoI NdeI NspI PflMI PfoI PpuMI
PshAI PspXI PstI PvuII SacI SalI SanDI SapI
ScaI SexAI SfcI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BciVI
BfrBI BglI BlpI Bme1580I BmtI BsaAI BsaHI BsiEI
BsiWI BsrFI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I
BtgZI CspCI DraIII EagI EcoRI FalI FseI FspI
FspAI HaeII HpaI KasI MluI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PsiI PspOMI PsrI PvuI RsrII SacII
SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI XbaI XmaI ZraI