Restriction map for
pF1KSDA0020
(Linear) (Six-base) MAPSORT of: KIAA0020.seq Check: 1128 from: 1 to: 1524
KIAA0020.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 647 1524
Size: 647 877
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1273 1369 1524
Size: 1273 96 155
Fragments arranged by size:
1273 155 96
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1232 1264 1336 1368 1524
Size: 1232 32 72 32 156
Fragments arranged by size:
1232 156 72 32 32
ApoI r'AATT_y
Cuts at: 0 241 1499 1524
Size: 241 1258 25
Fragments arranged by size:
1258 241 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 992 1025 1524
Size: 992 33 499
Fragments arranged by size:
992 499 33
BanI G'GyrC_C
Cuts at: 0 670 1375 1524
Size: 670 705 149
Fragments arranged by size:
705 670 149
BanII G_rGCy'C
Cuts at: 0 404 1025 1524
Size: 404 621 499
Fragments arranged by size:
621 499 404
BbeI G_GCGC'C
Cuts at: 0 674 1524
Size: 674 850
BbsI GAAGACnn'nnnn_
Cuts at: 0 748 1524
Size: 748 776
BglI GCCn_nnn'nGGC
Cuts at: 0 679 1174 1524
Size: 679 495 350
Fragments arranged by size:
679 495 350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1157 1384 1524
Size: 1025 132 227 140
Fragments arranged by size:
1025 227 140 132
BsaAI yAC'GTr
Cuts at: 0 303 1524
Size: 303 1221
BsaHI Gr'CG_yC
Cuts at: 0 671 1524
Size: 671 853
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1007 1037 1287 1317 1524
Size: 1007 30 250 30 207
Fragments arranged by size:
1007 250 207 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1387 1524
Size: 1387 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1424 1524
Size: 917 507 100
Fragments arranged by size:
917 507 100
BsiHKAI G_wGCw'C
Cuts at: 0 404 1025 1522 1524
Size: 404 621 497 2
Fragments arranged by size:
621 497 404 2
BsmI GAATG_Cn'
Cuts at: 0 1164 1524
Size: 1164 360
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1133 1524
Size: 1133 391
Bsp1286I G_dGCh'C
Cuts at: 0 404 1025 1522 1524
Size: 404 621 497 2
Fragments arranged by size:
621 497 404 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1067 1524
Size: 1067 457
BsrDI GCAATG_nn'
Cuts at: 0 862 1220 1524
Size: 862 358 304
Fragments arranged by size:
862 358 304
BssSI C'ACGA_G
Cuts at: 0 119 1524
Size: 119 1405
BstYI r'GATC_y
Cuts at: 0 927 1126 1524
Size: 927 199 398
Fragments arranged by size:
927 398 199
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1149 1524
Size: 1149 375
BtsI GCAGTG_nn'
Cuts at: 0 687 1524
Size: 687 837
DraI TTT'AAA
Cuts at: 0 294 1524
Size: 294 1230
EaeI y'GGCC_r
Cuts at: 0 680 768 1524
Size: 680 88 756
Fragments arranged by size:
756 680 88
EarI CTCTTCn'nnn_
Cuts at: 0 392 1524
Size: 392 1132
EcoICRI GAG'CTC
Cuts at: 0 402 1023 1524
Size: 402 621 501
Fragments arranged by size:
621 501 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1157 1273 1369 1384 1524
Size: 1025 132 116 96 15 140
Fragments arranged by size:
1025 140 132 116 96 15
EcoO109I rG'GnC_Cy
Cuts at: 0 900 1524
Size: 900 624
HaeII r_GCGC'y
Cuts at: 0 674 1524
Size: 674 850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 504 536 1007 1039 1524
Size: 504 32 471 32 485
Fragments arranged by size:
504 485 471 32 32
HindIII A'AGCT_T
Cuts at: 0 823 1449 1524
Size: 823 626 75
Fragments arranged by size:
823 626 75
KasI G'GCGC_C
Cuts at: 0 670 1524
Size: 670 854
MscI TGG'CCA
Cuts at: 0 682 770 1524
Size: 682 88 754
Fragments arranged by size:
754 682 88
MslI CAynn'nnrTG
Cuts at: 0 1196 1524
Size: 1196 328
NarI GG'CG_CC
Cuts at: 0 671 1524
Size: 671 853
NspI r_CATG'y
Cuts at: 0 325 390 1333 1524
Size: 325 65 943 191
Fragments arranged by size:
943 325 191 65
PflMI CCAn_nnn'nTGG
Cuts at: 0 1080 1524
Size: 1080 444
PmlI CAC'GTG
Cuts at: 0 303 1524
Size: 303 1221
PpuMI rG'GwC_Cy
Cuts at: 0 900 1524
Size: 900 624
PstI C_TGCA'G
Cuts at: 0 1239 1524
Size: 1239 285
SacI G_AGCT'C
Cuts at: 0 404 1025 1524
Size: 404 621 499
Fragments arranged by size:
621 499 404
SapI GCTCTTCn'nnn_
Cuts at: 0 392 1524
Size: 392 1132
SbfI CC_TGCA'GG
Cuts at: 0 1239 1524
Size: 1239 285
SfcI C'TryA_G
Cuts at: 0 1235 1524
Size: 1235 289
SfoI GGC'GCC
Cuts at: 0 672 1524
Size: 672 852
SphI G_CATG'C
Cuts at: 0 325 1524
Size: 325 1199
SspI AAT'ATT
Cuts at: 0 220 231 1524
Size: 220 11 1293
Fragments arranged by size:
1293 220 11
StyI C'CwwG_G
Cuts at: 0 710 1524
Size: 710 814
TatI w'GTAC_w
Cuts at: 0 908 1524
Size: 908 616
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1170 1524
Size: 1170 354
Enzymes that do cut:
AccI AcuI AloI ApoI BaeI BanI BanII BbeI
BbsI BglI BpmI BsaAI BsaHI BsaXI BseRI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BssSI BstYI
BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HindIII KasI MscI MslI NarI NspI
PflMI PmlI PpuMI PstI SacI SapI SbfI SfcI
SfoI SphI SspI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaBI BsaWI BseYI BsiEI BsiWI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI ZraI