Restriction map for pF1KSDA0022
 (Linear) (Six-base) MAPSORT of: KIAA0022.seq  Check: 3823  from: 1  to: 696



KIAA0022.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

BbsI GAAGACnn'nnnn_

Cuts at:      0     650     696 
   Size:        650      46
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     222     696 
   Size:        222     474
 
BmtI G_CTAG'C

Cuts at:      0     533     696 
   Size:        533     163
 
BseYI C'CCAG_C

Cuts at:      0      39     696 
   Size:         39     657
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     696 
   Size:        376     320
 
BsrBI CCG'CTC

Cuts at:      0     181     696 
   Size:        181     515
 
BsrDI GCAATG_nn'

Cuts at:      0     250     696 
   Size:        250     446
 
BsrGI T'GTAC_A

Cuts at:      0     575     696 
   Size:        575     121
 
BtgI C'CryG_G

Cuts at:      0     174     696 
   Size:        174     522
 
EcoRV GAT'ATC

Cuts at:      0     264     696 
   Size:        264     432
 
HincII GTy'rAC

Cuts at:      0     375     696 
   Size:        375     321
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     642     696 
   Size:        642      54
 
MspA1I CmG'CkG

Cuts at:      0      52     696 
   Size:         52     644
 
NcoI C'CATG_G

Cuts at:      0     174     696 
   Size:        174     522
 
NheI G'CTAG_C

Cuts at:      0     529     696 
   Size:        529     167
 
NspI r_CATG'y

Cuts at:      0     279     696 
   Size:        279     417
 
PsiI TTA'TAA

Cuts at:      0     638     696 
   Size:        638      58
 
SspI AAT'ATT

Cuts at:      0     487     696 
   Size:        487     209
 
StyI C'CwwG_G

Cuts at:      0     174     696 
   Size:        174     522
 
TatI w'GTAC_w

Cuts at:      0     166     575     696 
   Size:        166     409     121

  Fragments arranged by size: 

                 409     166     121
 

 Enzymes that do cut:

     BbsI    BciVI     BmtI    BseYI     BsgI    BsrBI    BsrDI    BsrGI
     BtgI    EcoRV   HincII     MmeI   MspA1I     NcoI     NheI     NspI
     PsiI     SspI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI
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