Restriction map for
pF1KSDA0023
(Linear) (Six-base) MAPSORT of: KIAA0023.seq Check: 338 from: 1 to: 6279
KIAA0023.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1395 2519 4695 6279
Size: 1395 1124 2176 1584
Fragments arranged by size:
2176 1584 1395 1124
AatII G_ACGT'C
Cuts at: 0 2971 4033 6279
Size: 2971 1062 2246
Fragments arranged by size:
2971 2246 1062
AccI GT'mk_AC
Cuts at: 0 1100 6279
Size: 1100 5179
Acc65I G'GTAC_C
Cuts at: 0 748 4843 5077 5398 6279
Size: 748 4095 234 321 881
Fragments arranged by size:
4095 881 748 321 234
AclI AA'CG_TT
Cuts at: 0 3310 6279
Size: 3310 2969
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2002 2383 2596 3104 3491 5154 5438 5577
Size: 2002 381 213 508 387 1663 284 139
Cuts at: 5577 6061 6279
Size: 484 218
Fragments arranged by size:
2002 1663 508 484 387 381 284 218
213 139
AfeI AGC'GCT
Cuts at: 0 62 6279
Size: 62 6217
AflII C'TTAA_G
Cuts at: 0 3451 6279
Size: 3451 2828
AleI CACnn'nnGTG
Cuts at: 0 1681 4951 6279
Size: 1681 3270 1328
Fragments arranged by size:
3270 1681 1328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1699 3257 6070 6279
Size: 1699 1558 2813 209
Fragments arranged by size:
2813 1699 1558 209
AlwNI CAG_nnn'CTG
Cuts at: 0 958 1338 1363 1543 2068 2484 2946 3412
Size: 958 380 25 180 525 416 462 466
Cuts at: 3412 3598 4732 4753 4933 5029 6279
Size: 186 1134 21 180 96 1250
Fragments arranged by size:
1250 1134 958 525 466 462 416 380
186 180 180 96 25 21
ApaLI G'TGCA_C
Cuts at: 0 3200 6279
Size: 3200 3079
ApoI r'AATT_y
Cuts at: 0 274 2405 2431 3444 6279
Size: 274 2131 26 1013 2835
Fragments arranged by size:
2835 2131 1013 274 26
AseI AT'TA_AT
Cuts at: 0 1007 1211 6279
Size: 1007 204 5068
Fragments arranged by size:
5068 1007 204
AvaI C'yCGr_G
Cuts at: 0 28 114 6279
Size: 28 86 6165
Fragments arranged by size:
6165 86 28
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 740 773 6279
Size: 740 33 5506
Fragments arranged by size:
5506 740 33
BamHI G'GATC_C
Cuts at: 0 2376 4195 5668 5887 6279
Size: 2376 1819 1473 219 392
Fragments arranged by size:
2376 1819 1473 392 219
BanI G'GyrC_C
Cuts at: 0 748 2411 2507 3279 3825 4843 5077 5254
Size: 748 1663 96 772 546 1018 234 177
Cuts at: 5254 5398 5907 6279
Size: 144 509 372
Fragments arranged by size:
1663 1018 772 748 546 509 372 234
177 144 96
BanII G_rGCy'C
Cuts at: 0 604 878 1434 1496 4094 5953 6279
Size: 604 274 556 62 2598 1859 326
Fragments arranged by size:
2598 1859 604 556 326 274 62
BbeI G_GCGC'C
Cuts at: 0 2415 6279
Size: 2415 3864
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 750 2602 2836 2909 3626 5294 6279
Size: 144 606 1852 234 73 717 1668 985
Fragments arranged by size:
1852 1668 985 717 606 234 144 73
BbvCI CC'TCA_GC
Cuts at: 0 5403 6279
Size: 5403 876
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 5952 5986 6279
Size: 32 5920 34 293
Fragments arranged by size:
5920 293 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 991 6279
Size: 991 5288
BclI T'GATC_A
Cuts at: 0 999 6279
Size: 999 5280
BglI GCCn_nnn'nGGC
Cuts at: 0 3235 3277 3462 5948 6279
Size: 3235 42 185 2486 331
Fragments arranged by size:
3235 2486 331 185 42
BlpI GC'TnA_GC
Cuts at: 0 2393 4874 4899 6279
Size: 2393 2481 25 1380
Fragments arranged by size:
2481 2393 1380 25
Bme1580I G_kGCm'C
Cuts at: 0 1946 2164 3204 4043 4289 5983 6279
Size: 1946 218 1040 839 246 1694 296
Fragments arranged by size:
1946 1694 1040 839 296 246 218
BmgBI CAC'GTC
Cuts at: 0 4799 6279
Size: 4799 1480
BmrI ACTGGGnnnn_n'
Cuts at: 0 1496 1933 2934 3038 3388 3400 5609 6279
Size: 1496 437 1001 104 350 12 2209 670
Fragments arranged by size:
2209 1496 1001 670 437 350 104 12
BmtI G_CTAG'C
Cuts at: 0 3140 6279
Size: 3140 3139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2408 6047 6279
Size: 2408 3639 232
Fragments arranged by size:
3639 2408 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 788 1048 1142 1964 2840 2968 3938
Size: 618 170 260 94 822 876 128 970
Cuts at: 3938 4773 5262 5804 5840 6279
Size: 835 489 542 36 439
Fragments arranged by size:
970 876 835 822 618 542 489 439
260 170 128 94 36
Bpu10I CC'TnA_GC
Cuts at: 0 1922 5052 5403 6279
Size: 1922 3130 351 876
Fragments arranged by size:
3130 1922 876 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1307 2305 3137 3311 4725 5247 6279
Size: 318 989 998 832 174 1414 522 1032
Fragments arranged by size:
1414 1032 998 989 832 522 318 174
BsaI GGTCTCn'nnnn_
Cuts at: 0 2227 3440 4470 6279
Size: 2227 1213 1030 1809
Fragments arranged by size:
2227 1809 1213 1030
BsaBI GATnn'nnATC
Cuts at: 0 2063 2480 6279
Size: 2063 417 3799
Fragments arranged by size:
3799 2063 417
BsaHI Gr'CG_yC
Cuts at: 0 2412 2968 4030 6279
Size: 2412 556 1062 2249
Fragments arranged by size:
2412 2249 1062 556
BsaWI w'CCGG_w
Cuts at: 0 3478 6279
Size: 3478 2801
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 1082 1112 1134 1164 4486 4516
Size: 437 30 615 30 22 30 3322 30
Cuts at: 4516 5372 5402 5780 5810 5951 5981 6279
Size: 856 30 378 30 141 30 298
Fragments arranged by size:
3322 856 615 437 378 298 141 30
30 30 30 30 30 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1308 2104 2185 4507 4514 5801 6092 6279
Size: 1308 796 81 2322 7 1287 291 187
Fragments arranged by size:
2322 1308 1287 796 291 187 81 7
BseYI C'CCAG_C
Cuts at: 0 3885 4016 4508 4568 4641 5067 5928 6279
Size: 3885 131 492 60 73 426 861 351
Fragments arranged by size:
3885 861 492 426 351 131 73 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 876 3361 3635 4211 4766 6279
Size: 876 2485 274 576 555 1513
Fragments arranged by size:
2485 1513 876 576 555 274
BsiHKAI G_wGCw'C
Cuts at: 0 3204 6279
Size: 3204 3075
BsmI GAATG_Cn'
Cuts at: 0 2681 3697 5914 6229 6279
Size: 2681 1016 2217 315 50
Fragments arranged by size:
2681 2217 1016 315 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 806 1613 6279
Size: 806 807 4666
Fragments arranged by size:
4666 807 806
Bsp1286I G_dGCh'C
Cuts at: 0 604 878 1434 1496 1946 2164 3204 4043
Size: 604 274 556 62 450 218 1040 839
Cuts at: 4043 4094 4289 5953 5983 6279
Size: 51 195 1664 30 296
Fragments arranged by size:
1664 1040 839 604 556 450 296 274
218 195 62 51 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1395 2519 3807 4695 6279
Size: 1395 1124 1288 888 1584
Fragments arranged by size:
1584 1395 1288 1124 888
BsrBI CCG'CTC
Cuts at: 0 33 6097 6279
Size: 33 6064 182
Fragments arranged by size:
6064 182 33
BsrDI GCAATG_nn'
Cuts at: 0 1397 1718 2724 6279
Size: 1397 321 1006 3555
Fragments arranged by size:
3555 1397 1006 321
BstAPI GCAn_nnn'nTGC
Cuts at: 0 958 1363 3070 4627 6279
Size: 958 405 1707 1557 1652
Fragments arranged by size:
1707 1652 1557 958 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5812 6279
Size: 5812 467
BstYI r'GATC_y
Cuts at: 0 2064 2376 2845 4195 5668 5887 6124 6279
Size: 2064 312 469 1350 1473 219 237 155
Fragments arranged by size:
2064 1473 1350 469 312 237 219 155
Bsu36I CC'TnA_GG
Cuts at: 0 92 3759 4044 6279
Size: 92 3667 285 2235
Fragments arranged by size:
3667 2235 285 92
BtgI C'CryG_G
Cuts at: 0 488 572 1085 3017 4649 6279
Size: 488 84 513 1932 1632 1630
Fragments arranged by size:
1932 1632 1630 513 488 84
BtsI GCAGTG_nn'
Cuts at: 0 629 2849 2996 3644 4592 4931 5154 5981
Size: 629 2220 147 648 948 339 223 827
Cuts at: 5981 6279
Size: 298
Fragments arranged by size:
2220 948 827 648 629 339 298 223
147
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1097 1132 4785 4820 5820 5855 6279
Size: 1097 35 3653 35 1000 35 424
Fragments arranged by size:
3653 1097 1000 424 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2601 3106 3396 6279
Size: 2601 505 290 2883
Fragments arranged by size:
2883 2601 505 290
DrdI GACnn_nn'nnGTC
Cuts at: 0 2241 4462 6279
Size: 2241 2221 1817
Fragments arranged by size:
2241 2221 1817
EaeI y'GGCC_r
Cuts at: 0 2819 3269 3954 4757 5331 5523 5651 6017
Size: 2819 450 685 803 574 192 128 366
Cuts at: 6017 6132 6279
Size: 115 147
Fragments arranged by size:
2819 803 685 574 450 366 192 147
128 115
EarI CTCTTCn'nnn_
Cuts at: 0 569 1151 1809 2114 2578 4412 5544 5571
Size: 569 582 658 305 464 1834 1132 27
Cuts at: 5571 6279
Size: 708
Fragments arranged by size:
1834 1132 708 658 582 569 464 305
27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2611 6279
Size: 2611 3668
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 788 1048 1142 1964 2002 2383 2596
Size: 618 170 260 94 822 38 381 213
Cuts at: 2596 2840 2968 3104 3491 3938 4773 5154 5262
Size: 244 128 136 387 447 835 381 108
Cuts at: 5262 5438 5577 5804 5840 6061 6279
Size: 176 139 227 36 221 218
Fragments arranged by size:
835 822 618 447 387 381 381 260
244 227 221 218 213 176 170 139
136 128 108 94 38 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3758 6279
Size: 3758 2521
EcoO109I rG'GnC_Cy
Cuts at: 0 788 1540 3000 4656 4750 6011 6270 6279
Size: 788 752 1460 1656 94 1261 259 9
Fragments arranged by size:
1656 1460 1261 788 752 259 94 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1740 1774 2413 3740 6279
Size: 1740 34 639 1327 2539
Fragments arranged by size:
2539 1740 1327 639 34
HaeII r_GCGC'y
Cuts at: 0 64 2415 6279
Size: 64 2351 3864
Fragments arranged by size:
3864 2351 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1134 1166 1962 1994 2577 2609
Size: 891 32 211 32 796 32 583 32
Cuts at: 2609 3258 3290 3685 3717 4486 4518 5372 5404
Size: 649 32 395 32 769 32 854 32
Cuts at: 5404 6109 6141 6279
Size: 705 32 138
Fragments arranged by size:
891 854 796 769 705 649 583 395
211 138 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1755 3401 6279
Size: 1755 1646 2878
Fragments arranged by size:
2878 1755 1646
HindIII A'AGCT_T
Cuts at: 0 427 3503 5450 6279
Size: 427 3076 1947 829
Fragments arranged by size:
3076 1947 829 427
KasI G'GCGC_C
Cuts at: 0 2411 6279
Size: 2411 3868
KpnI G_GTAC'C
Cuts at: 0 752 4847 5081 5402 6279
Size: 752 4095 234 321 877
Fragments arranged by size:
4095 877 752 321 234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1541 2096 4354 6058 6279
Size: 158 1383 555 2258 1704 221
Fragments arranged by size:
2258 1704 1383 555 221 158
MscI TGG'CCA
Cuts at: 0 2821 3271 3956 5333 5525 6019 6134 6279
Size: 2821 450 685 1377 192 494 115 145
Fragments arranged by size:
2821 1377 685 494 450 192 145 115
MslI CAynn'nnrTG
Cuts at: 0 1681 2423 4951 6279
Size: 1681 742 2528 1328
Fragments arranged by size:
2528 1681 1328 742
MspA1I CmG'CkG
Cuts at: 0 618 890 1354 1627 1822 1984 3241 3286
Size: 618 272 464 273 195 162 1257 45
Cuts at: 3286 3532 4315 4402 4732 5029 5407 5989 6279
Size: 246 783 87 330 297 378 582 290
Fragments arranged by size:
1257 783 618 582 464 378 330 297
290 273 272 246 195 162 87 45
NarI GG'CG_CC
Cuts at: 0 2412 6279
Size: 2412 3867
NcoI C'CATG_G
Cuts at: 0 488 1085 6279
Size: 488 597 5194
Fragments arranged by size:
5194 597 488
NheI G'CTAG_C
Cuts at: 0 3136 6279
Size: 3136 3143
NruI TCG'CGA
Cuts at: 0 6215 6279
Size: 6215 64
PasI CC'CwG_GG
Cuts at: 0 5089 5893 6279
Size: 5089 804 386
Fragments arranged by size:
5089 804 386
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 1543 2946 5074 5863 6279
Size: 291 1252 1403 2128 789 416
Fragments arranged by size:
2128 1403 1252 789 416 291
PpuMI rG'GwC_Cy
Cuts at: 0 788 4656 6011 6270 6279
Size: 788 3868 1355 259 9
Fragments arranged by size:
3868 1355 788 259 9
PshAI GACnn'nnGTC
Cuts at: 0 4720 6279
Size: 4720 1559
PsiI TTA'TAA
Cuts at: 0 2363 6279
Size: 2363 3916
PspXI vC'TCGA_Gb
Cuts at: 0 114 6279
Size: 114 6165
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 5265 5297 5463 5495 6279
Size: 5265 32 166 32 784
Fragments arranged by size:
5265 784 166 32 32
PstI C_TGCA'G
Cuts at: 0 783 1353 1359 1605 4578 4599 4632 4827
Size: 783 570 6 246 2973 21 33 195
Cuts at: 4827 5988 6279
Size: 1161 291
Fragments arranged by size:
2973 1161 783 570 291 246 195 33
21 6
PvuII CAG'CTG
Cuts at: 0 618 890 1354 1627 1822 3241 3286 3532
Size: 618 272 464 273 195 1419 45 246
Cuts at: 3532 4315 4732 5029 5407 5989 6279
Size: 783 417 297 378 582 290
Fragments arranged by size:
1419 783 618 582 464 417 378 297
290 273 272 246 195 45
SanDI GG'GwC_CC
Cuts at: 0 788 4656 6011 6270 6279
Size: 788 3868 1355 259 9
Fragments arranged by size:
3868 1355 788 259 9
ScaI AGT'ACT
Cuts at: 0 1299 4608 5505 6279
Size: 1299 3309 897 774
Fragments arranged by size:
3309 1299 897 774
SexAI A'CCwGG_T
Cuts at: 0 2597 3083 3102 6279
Size: 2597 486 19 3177
Fragments arranged by size:
3177 2597 486 19
SfcI C'TryA_G
Cuts at: 0 779 1349 1355 1601 3374 4310 4574 4595
Size: 779 570 6 246 1773 936 264 21
Cuts at: 4595 4628 4823 5984 6279
Size: 33 195 1161 295
Fragments arranged by size:
1773 1161 936 779 570 295 264 246
195 33 21 6
SfoI GGC'GCC
Cuts at: 0 2413 6279
Size: 2413 3866
SmlI C'TyrA_G
Cuts at: 0 114 297 1322 2284 3152 3326 3451 4740
Size: 114 183 1025 962 868 174 125 1289
Cuts at: 4740 5262 6279
Size: 522 1017
Fragments arranged by size:
1289 1025 1017 962 868 522 183 174
125 114
SpeI A'CTAG_T
Cuts at: 0 4009 4246 4609 6279
Size: 4009 237 363 1670
Fragments arranged by size:
4009 1670 363 237
StuI AGG'CCT
Cuts at: 0 5122 6279
Size: 5122 1157
StyI C'CwwG_G
Cuts at: 0 104 252 488 1085 5601 5805 6068 6129
Size: 104 148 236 597 4516 204 263 61
Cuts at: 6129 6279
Size: 150
Fragments arranged by size:
4516 597 263 236 204 150 148 104
61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 863 1980 3407 5194 6279
Size: 863 1117 1427 1787 1085
Fragments arranged by size:
1787 1427 1117 1085 863
TatI w'GTAC_w
Cuts at: 0 1297 4606 5503 6279
Size: 1297 3309 897 776
Fragments arranged by size:
3309 1297 897 776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 437 469 724 756 2698 2730 2740 2772
Size: 437 32 255 32 1942 32 10 32
Cuts at: 2772 3107 3139 4311 4343 5780 5812 5951 5983
Size: 335 32 1172 32 1437 32 139 32
Cuts at: 5983 6279
Size: 296
Fragments arranged by size:
1942 1437 1172 437 335 296 255 139
32 32 32 32 32 32 32 32
10
XbaI T'CTAG_A
Cuts at: 0 2460 4488 6279
Size: 2460 2028 1791
Fragments arranged by size:
2460 2028 1791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 6173 6257 6279
Size: 6173 84 22
Fragments arranged by size:
6173 84 22
XhoI C'TCGA_G
Cuts at: 0 114 6279
Size: 114 6165
XmnI GAAnn'nnTTC
Cuts at: 0 3936 4559 6279
Size: 3936 623 1720
Fragments arranged by size:
3936 1720 623
ZraI GAC'GTC
Cuts at: 0 2969 4031 6279
Size: 2969 1062 2248
Fragments arranged by size:
2969 2248 1062
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AleI AlfI AlwNI ApaLI ApoI AseI AvaI BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
CspCI DraIII DrdI EaeI EarI EciI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII HindIII KasI KpnI
MmeI MscI MslI MspA1I NarI NcoI NheI NruI
PasI PflMI PpuMI PshAI PsiI PspXI PsrI PstI
PvuII SanDI ScaI SexAI SfcI SfoI SmlI SpeI
StuI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AflIII AgeI AhdI AloI ApaI AscI AsiSI AvrII
BfrBI BglII BsaAI BsiEI BsiWI BspEI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
DraI EagI EcoICRI EcoRI EcoRV FseI FspI FspAI
HpaI MfeI MluI NaeI NdeI NgoMIV NotI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PspOMI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
Tth111I XmaI