Restriction map for
pF1KSDA0026
(Linear) (Six-base) MAPSORT of: KIAA0026.seq Check: 9390 from: 1 to: 864
KIAA0026.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 398 864
Size: 188 210 466
Fragments arranged by size:
466 210 188
AhdI GACnn_n'nnGTC
Cuts at: 0 131 864
Size: 131 733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 607 864
Size: 607 257
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 864
Size: 146 32 686
Fragments arranged by size:
686 146 32
ApaI G_GGCC'C
Cuts at: 0 309 864
Size: 309 555
ApoI r'AATT_y
Cuts at: 0 601 795 864
Size: 601 194 69
Fragments arranged by size:
601 194 69
AvaI C'yCGr_G
Cuts at: 0 308 864
Size: 308 556
BamHI G'GATC_C
Cuts at: 0 208 864
Size: 208 656
BanII G_rGCy'C
Cuts at: 0 309 864
Size: 309 555
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 226 247 864
Size: 130 96 21 617
Fragments arranged by size:
617 130 96 21
BbvCI CC'TCA_GC
Cuts at: 0 284 864
Size: 284 580
Bme1580I G_kGCm'C
Cuts at: 0 309 586 864
Size: 309 277 278
Fragments arranged by size:
309 278 277
BmrI ACTGGGnnnn_n'
Cuts at: 0 417 609 864
Size: 417 192 255
Fragments arranged by size:
417 255 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 864
Size: 496 368
Bpu10I CC'TnA_GC
Cuts at: 0 284 864
Size: 284 580
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 146 176 864
Size: 146 30 688
Fragments arranged by size:
688 146 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 349 493 864
Size: 102 247 144 371
Fragments arranged by size:
371 247 144 102
BsiHKAI G_wGCw'C
Cuts at: 0 675 864
Size: 675 189
Bsp1286I G_dGCh'C
Cuts at: 0 309 586 675 864
Size: 309 277 89 189
Fragments arranged by size:
309 277 189 89
BsrDI GCAATG_nn'
Cuts at: 0 716 864
Size: 716 148
BssSI C'ACGA_G
Cuts at: 0 29 864
Size: 29 835
BstXI CCAn_nnnn'nTGG
Cuts at: 0 173 667 864
Size: 173 494 197
Fragments arranged by size:
494 197 173
BstYI r'GATC_y
Cuts at: 0 208 864
Size: 208 656
Bsu36I CC'TnA_GG
Cuts at: 0 114 864
Size: 114 750
BtgI C'CryG_G
Cuts at: 0 391 864
Size: 391 473
EarI CTCTTCn'nnn_
Cuts at: 0 47 864
Size: 47 817
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 398 496 864
Size: 188 210 98 368
Fragments arranged by size:
368 210 188 98
EcoO109I rG'GnC_Cy
Cuts at: 0 107 305 611 864
Size: 107 198 306 253
Fragments arranged by size:
306 253 198 107
EcoRI G'AATT_C
Cuts at: 0 795 864
Size: 795 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 117 149 864
Size: 117 32 715
Fragments arranged by size:
715 117 32
MslI CAynn'nnrTG
Cuts at: 0 644 864
Size: 644 220
MspA1I CmG'CkG
Cuts at: 0 130 435 591 864
Size: 130 305 156 273
Fragments arranged by size:
305 273 156 130
NcoI C'CATG_G
Cuts at: 0 391 864
Size: 391 473
NspI r_CATG'y
Cuts at: 0 87 864
Size: 87 777
PfoI T'CCnGG_A
Cuts at: 0 246 864
Size: 246 618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 146 178 864
Size: 146 32 686
Fragments arranged by size:
686 146 32
PspOMI G'GGCC_C
Cuts at: 0 305 864
Size: 305 559
PstI C_TGCA'G
Cuts at: 0 48 192 864
Size: 48 144 672
Fragments arranged by size:
672 144 48
PvuII CAG'CTG
Cuts at: 0 130 435 591 864
Size: 130 305 156 273
Fragments arranged by size:
305 273 156 130
SfcI C'TryA_G
Cuts at: 0 44 188 864
Size: 44 144 676
Fragments arranged by size:
676 144 44
SmaI CCC'GGG
Cuts at: 0 310 864
Size: 310 554
SrfI GCCC'GGGC
Cuts at: 0 310 864
Size: 310 554
SspI AAT'ATT
Cuts at: 0 565 864
Size: 565 299
StyI C'CwwG_G
Cuts at: 0 391 864
Size: 391 473
XmaI C'CCGG_G
Cuts at: 0 308 864
Size: 308 556
Enzymes that do cut:
AcuI AhdI AlfI AloI ApaI ApoI AvaI BamHI
BanII BbsI BbvCI Bme1580I BmrI BpmI Bpu10I BsaXI
BseRI BsiHKAI Bsp1286I BsrDI BssSI BstXI BstYI Bsu36I
BtgI EarI Eco57MI EcoO109I EcoRI Hin4I MslI MspA1I
NcoI NspI PfoI PpiI PspOMI PstI PvuII SfcI
SmaI SrfI SspI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BanI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI StuI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI