Restriction map for
pF1KSDA0027
(Linear) (Six-base) MAPSORT of: KIAA0027.seq Check: 644 from: 1 to: 1131
KIAA0027.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1096 1131
Size: 123 973 35
Fragments arranged by size:
973 123 35
AflIII A'CryG_T
Cuts at: 0 221 370 1131
Size: 221 149 761
Fragments arranged by size:
761 221 149
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 282 1131
Size: 282 849
AlwNI CAG_nnn'CTG
Cuts at: 0 826 1131
Size: 826 305
AvaI C'yCGr_G
Cuts at: 0 195 575 631 1131
Size: 195 380 56 500
Fragments arranged by size:
500 380 195 56
BamHI G'GATC_C
Cuts at: 0 701 1131
Size: 701 430
BanI G'GyrC_C
Cuts at: 0 961 1131
Size: 961 170
BanII G_rGCy'C
Cuts at: 0 529 1072 1131
Size: 529 543 59
Fragments arranged by size:
543 529 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 150 1131
Size: 150 981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 90 108 124 142 1131
Size: 90 18 16 18 989
Fragments arranged by size:
989 90 18 18 16
BclI T'GATC_A
Cuts at: 0 479 1131
Size: 479 652
Bme1580I G_kGCm'C
Cuts at: 0 964 1131
Size: 964 167
BmgBI CAC'GTC
Cuts at: 0 152 803 1131
Size: 152 651 328
Fragments arranged by size:
651 328 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 285 475 1122 1131
Size: 73 212 190 647 9
Fragments arranged by size:
647 212 190 73 9
BsaAI yAC'GTr
Cuts at: 0 371 1131
Size: 371 760
BsaWI w'CCGG_w
Cuts at: 0 38 1131
Size: 38 1093
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 517 1131
Size: 37 480 614
Fragments arranged by size:
614 480 37
BseYI C'CCAG_C
Cuts at: 0 757 1051 1131
Size: 757 294 80
Fragments arranged by size:
757 294 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 1131
Size: 473 658
BsiHKAI G_wGCw'C
Cuts at: 0 935 1131
Size: 935 196
BsmBI CGTCTCn'nnnn_
Cuts at: 0 303 809 1131
Size: 303 506 322
Fragments arranged by size:
506 322 303
Bsp1286I G_dGCh'C
Cuts at: 0 529 935 964 1072 1131
Size: 529 406 29 108 59
Fragments arranged by size:
529 406 108 59 29
BspHI T'CATG_A
Cuts at: 0 848 1131
Size: 848 283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 1131
Size: 116 1015
BsrBI CCG'CTC
Cuts at: 0 57 1131
Size: 57 1074
BsrDI GCAATG_nn'
Cuts at: 0 641 728 1131
Size: 641 87 403
Fragments arranged by size:
641 403 87
BsrFI r'CCGG_y
Cuts at: 0 62 946 1131
Size: 62 884 185
Fragments arranged by size:
884 185 62
BstEII G'GTnAC_C
Cuts at: 0 189 1131
Size: 189 942
BstXI CCAn_nnnn'nTGG
Cuts at: 0 552 741 1131
Size: 552 189 390
Fragments arranged by size:
552 390 189
BstYI r'GATC_y
Cuts at: 0 701 1131
Size: 701 430
BtgI C'CryG_G
Cuts at: 0 473 1131
Size: 473 658
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 468 1131
Size: 468 663
DraIII CAC_nnn'GTG
Cuts at: 0 371 1131
Size: 371 760
EaeI y'GGCC_r
Cuts at: 0 470 1061 1131
Size: 470 591 70
Fragments arranged by size:
591 470 70
EarI CTCTTCn'nnn_
Cuts at: 0 115 1131
Size: 115 1016
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 123 285 475 1096 1122 1131
Size: 73 50 162 190 621 26 9
Fragments arranged by size:
621 190 162 73 50 26 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 940 1131
Size: 940 191
EcoO109I rG'GnC_Cy
Cuts at: 0 579 777 1131
Size: 579 198 354
Fragments arranged by size:
579 354 198
EcoRV GAT'ATC
Cuts at: 0 832 1131
Size: 832 299
FseI GG_CCGG'CC
Cuts at: 0 950 1131
Size: 950 181
HaeII r_GCGC'y
Cuts at: 0 545 1047 1131
Size: 545 502 84
Fragments arranged by size:
545 502 84
MfeI C'AATT_G
Cuts at: 0 281 1131
Size: 281 850
MscI TGG'CCA
Cuts at: 0 1063 1131
Size: 1063 68
MspA1I CmG'CkG
Cuts at: 0 114 810 1131
Size: 114 696 321
Fragments arranged by size:
696 321 114
NaeI GCC'GGC
Cuts at: 0 64 948 1131
Size: 64 884 183
Fragments arranged by size:
884 183 64
NgoMIV G'CCGG_C
Cuts at: 0 62 946 1131
Size: 62 884 185
Fragments arranged by size:
884 185 62
PfoI T'CCnGG_A
Cuts at: 0 301 1131
Size: 301 830
PpuMI rG'GwC_Cy
Cuts at: 0 579 777 1131
Size: 579 198 354
Fragments arranged by size:
579 354 198
PshAI GACnn'nnGTC
Cuts at: 0 154 1131
Size: 154 977
PstI C_TGCA'G
Cuts at: 0 113 261 944 1007 1131
Size: 113 148 683 63 124
Fragments arranged by size:
683 148 124 113 63
PvuII CAG'CTG
Cuts at: 0 114 1131
Size: 114 1017
SfcI C'TryA_G
Cuts at: 0 109 257 940 1003 1131
Size: 109 148 683 63 128
Fragments arranged by size:
683 148 128 109 63
SpeI A'CTAG_T
Cuts at: 0 396 1131
Size: 396 735
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1039 1131
Size: 1039 92
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 765 1131
Size: 765 366
XmnI GAAnn'nnTTC
Cuts at: 0 223 1081 1131
Size: 223 858 50
Fragments arranged by size:
858 223 50
Enzymes that do cut:
AcuI AflIII AlfI AlwNI AvaI BamHI BanI BanII
BbsI BcgI BclI Bme1580I BmgBI BpmI BsaAI BsaWI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI
DraIII EaeI EarI Eco57MI EcoNI EcoO109I EcoRV FseI
HaeII MfeI MscI MspA1I NaeI NgoMIV PfoI PpuMI
PshAI PstI PvuII SfcI SpeI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BciVI BfrBI BglI
BglII BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BsiEI BsiWI BsmI BspEI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoRI FalI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SphI
SrfI SspI StuI StyI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI