Restriction map for
pF1KSDA0031
(Linear) (Six-base) MAPSORT of: KIAA0031.seq Check: 6240 from: 1 to: 2916
KIAA0031.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 2447 2916
Size: 2447 469
Acc65I G'GTAC_C
Cuts at: 0 1607 2916
Size: 1607 1309
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 1243 2916
Size: 820 423 1673
Fragments arranged by size:
1673 820 423
AfeI AGC'GCT
Cuts at: 0 478 2916
Size: 478 2438
AflIII A'CryG_T
Cuts at: 0 427 621 1295 2916
Size: 427 194 674 1621
Fragments arranged by size:
1621 674 427 194
AgeI A'CCGG_T
Cuts at: 0 1360 2916
Size: 1360 1556
AleI CACnn'nnGTG
Cuts at: 0 553 1858 2916
Size: 553 1305 1058
Fragments arranged by size:
1305 1058 553
AlwNI CAG_nnn'CTG
Cuts at: 0 760 947 2916
Size: 760 187 1969
Fragments arranged by size:
1969 760 187
ApaI G_GGCC'C
Cuts at: 0 2332 2916
Size: 2332 584
ApoI r'AATT_y
Cuts at: 0 301 627 1012 2794 2851 2916
Size: 301 326 385 1782 57 65
Fragments arranged by size:
1782 385 326 301 65 57
AseI AT'TA_AT
Cuts at: 0 862 1001 2916
Size: 862 139 1915
Fragments arranged by size:
1915 862 139
AvaI C'yCGr_G
Cuts at: 0 1703 2789 2916
Size: 1703 1086 127
Fragments arranged by size:
1703 1086 127
BanI G'GyrC_C
Cuts at: 0 1330 1495 1607 2916
Size: 1330 165 112 1309
Fragments arranged by size:
1330 1309 165 112
BanII G_rGCy'C
Cuts at: 0 383 964 1904 2332 2399 2916
Size: 383 581 940 428 67 517
Fragments arranged by size:
940 581 517 428 383 67
BbeI G_GCGC'C
Cuts at: 0 1334 2916
Size: 1334 1582
BbsI GAAGACnn'nnnn_
Cuts at: 0 257 1565 1687 2691 2916
Size: 257 1308 122 1004 225
Fragments arranged by size:
1308 1004 257 225 122
BbvCI CC'TCA_GC
Cuts at: 0 695 2916
Size: 695 2221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2838 2872 2916
Size: 2838 34 44
Fragments arranged by size:
2838 44 34
BclI T'GATC_A
Cuts at: 0 725 1665 2801 2916
Size: 725 940 1136 115
Fragments arranged by size:
1136 940 725 115
BfrBI ATG'CAT
Cuts at: 0 1923 2916
Size: 1923 993
BglII A'GATC_T
Cuts at: 0 93 489 974 2916
Size: 93 396 485 1942
Fragments arranged by size:
1942 485 396 93
BlpI GC'TnA_GC
Cuts at: 0 2066 2916
Size: 2066 850
Bme1580I G_kGCm'C
Cuts at: 0 1894 2332 2567 2916
Size: 1894 438 235 349
Fragments arranged by size:
1894 438 349 235
BmgBI CAC'GTC
Cuts at: 0 1161 2916
Size: 1161 1755
BmrI ACTGGGnnnn_n'
Cuts at: 0 930 1537 1653 1904 1966 2916
Size: 930 607 116 251 62 950
Fragments arranged by size:
950 930 607 251 116 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1578 2916
Size: 1578 1338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 814 912 1567 2916
Size: 599 215 98 655 1349
Fragments arranged by size:
1349 655 599 215 98
Bpu10I CC'TnA_GC
Cuts at: 0 695 1246 2916
Size: 695 551 1670
Fragments arranged by size:
1670 695 551
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1999 2095 2916
Size: 1999 96 821
Fragments arranged by size:
1999 821 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 226 2916
Size: 79 147 2690
Fragments arranged by size:
2690 147 79
BsaAI yAC'GTr
Cuts at: 0 2123 2568 2916
Size: 2123 445 348
Fragments arranged by size:
2123 445 348
BsaBI GATnn'nnATC
Cuts at: 0 1954 2171 2916
Size: 1954 217 745
Fragments arranged by size:
1954 745 217
BsaHI Gr'CG_yC
Cuts at: 0 1331 2916
Size: 1331 1585
BsaWI w'CCGG_w
Cuts at: 0 1360 2660 2916
Size: 1360 1300 256
Fragments arranged by size:
1360 1300 256
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 1231 1729 1879 2462 2768 2916
Size: 223 1008 498 150 583 306 148
Fragments arranged by size:
1008 583 498 306 223 150 148
BseYI C'CCAG_C
Cuts at: 0 957 1507 2139 2916
Size: 957 550 632 777
Fragments arranged by size:
957 777 632 550
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1324 2386 2916
Size: 760 564 1062 530
Fragments arranged by size:
1062 760 564 530
BsiHKAI G_wGCw'C
Cuts at: 0 383 1352 1904 2903 2916
Size: 383 969 552 999 13
Fragments arranged by size:
999 969 552 383 13
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1984 2532 2916
Size: 1984 548 384
Fragments arranged by size:
1984 548 384
Bsp1286I G_dGCh'C
Cuts at: 0 383 964 1352 1894 1904 2332 2399 2567
Size: 383 581 388 542 10 428 67 168
Cuts at: 2567 2903 2916
Size: 336 13
Fragments arranged by size:
581 542 428 388 383 336 168 67
13 10
BspEI T'CCGG_A
Cuts at: 0 2660 2916
Size: 2660 256
BspHI T'CATG_A
Cuts at: 0 141 2798 2916
Size: 141 2657 118
Fragments arranged by size:
2657 141 118
BsrBI CCG'CTC
Cuts at: 0 720 2916
Size: 720 2196
BsrDI GCAATG_nn'
Cuts at: 0 1716 2916
Size: 1716 1200
BsrFI r'CCGG_y
Cuts at: 0 782 1360 2916
Size: 782 578 1556
Fragments arranged by size:
1556 782 578
BsrGI T'GTAC_A
Cuts at: 0 1442 2603 2916
Size: 1442 1161 313
Fragments arranged by size:
1442 1161 313
BstAPI GCAn_nnn'nTGC
Cuts at: 0 738 1929 2718 2916
Size: 738 1191 789 198
Fragments arranged by size:
1191 789 738 198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1860 2232 2916
Size: 1860 372 684
Fragments arranged by size:
1860 684 372
BstYI r'GATC_y
Cuts at: 0 93 360 489 974 1136 1592 2172 2369
Size: 93 267 129 485 162 456 580 197
Cuts at: 2369 2916
Size: 547
Fragments arranged by size:
580 547 485 456 267 197 162 129
93
Bsu36I CC'TnA_GG
Cuts at: 0 224 2916
Size: 224 2692
BtgI C'CryG_G
Cuts at: 0 417 1177 1577 2916
Size: 417 760 400 1339
Fragments arranged by size:
1339 760 417 400
DraIII CAC_nnn'GTG
Cuts at: 0 764 843 2916
Size: 764 79 2073
Fragments arranged by size:
2073 764 79
DrdI GACnn_nn'nnGTC
Cuts at: 0 1975 2916
Size: 1975 941
EaeI y'GGCC_r
Cuts at: 0 1479 1601 2549 2627 2916
Size: 1479 122 948 78 289
Fragments arranged by size:
1479 948 289 122 78
EarI CTCTTCn'nnn_
Cuts at: 0 517 601 2276 2916
Size: 517 84 1675 640
Fragments arranged by size:
1675 640 517 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 373 1225 2743 2916
Size: 373 852 1518 173
Fragments arranged by size:
1518 852 373 173
EcoICRI GAG'CTC
Cuts at: 0 381 1902 2916
Size: 381 1521 1014
Fragments arranged by size:
1521 1014 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 814 820 912 1243 1567 2916
Size: 599 215 6 92 331 324 1349
Fragments arranged by size:
1349 599 331 324 215 92 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1144 1210 1245 1384 1822 2089 2916
Size: 1144 66 35 139 438 267 827
Fragments arranged by size:
1144 827 438 267 139 66 35
EcoO109I rG'GnC_Cy
Cuts at: 0 1077 2082 2328 2329 2511 2823 2916
Size: 1077 1005 246 1 182 312 93
Fragments arranged by size:
1077 1005 312 246 182 93 1
EcoRI G'AATT_C
Cuts at: 0 2794 2916
Size: 2794 122
FspI TGC'GCA
Cuts at: 0 658 1822 2916
Size: 658 1164 1094
Fragments arranged by size:
1164 1094 658
HaeII r_GCGC'y
Cuts at: 0 480 1334 2916
Size: 480 854 1582
Fragments arranged by size:
1582 854 480
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 78 110 2512 2544 2916
Size: 78 32 2402 32 372
Fragments arranged by size:
2402 372 78 32 32
HincII GTy'rAC
Cuts at: 0 1785 1830 2916
Size: 1785 45 1086
Fragments arranged by size:
1785 1086 45
HindIII A'AGCT_T
Cuts at: 0 2615 2916
Size: 2615 301
KasI G'GCGC_C
Cuts at: 0 1330 2916
Size: 1330 1586
KpnI G_GTAC'C
Cuts at: 0 1611 2916
Size: 1611 1305
MfeI C'AATT_G
Cuts at: 0 1673 2916
Size: 1673 1243
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1400 1755 2916
Size: 835 565 355 1161
Fragments arranged by size:
1161 835 565 355
MscI TGG'CCA
Cuts at: 0 1603 2551 2916
Size: 1603 948 365
Fragments arranged by size:
1603 948 365
MslI CAynn'nnrTG
Cuts at: 0 146 310 553 1300 1618 1858 1960 2233
Size: 146 164 243 747 318 240 102 273
Cuts at: 2233 2916
Size: 683
Fragments arranged by size:
747 683 318 273 243 240 164 146
102
MspA1I CmG'CkG
Cuts at: 0 649 2916
Size: 649 2267
NarI GG'CG_CC
Cuts at: 0 1331 2916
Size: 1331 1585
NcoI C'CATG_G
Cuts at: 0 417 2916
Size: 417 2499
NsiI A_TGCA'T
Cuts at: 0 1925 2916
Size: 1925 991
NspI r_CATG'y
Cuts at: 0 431 625 736 873 1299 1882 2916
Size: 431 194 111 137 426 583 1034
Fragments arranged by size:
1034 583 431 426 194 137 111
PasI CC'CwG_GG
Cuts at: 0 157 2673 2916
Size: 157 2516 243
Fragments arranged by size:
2516 243 157
PciI A'CATG_T
Cuts at: 0 427 621 1295 2916
Size: 427 194 674 1621
Fragments arranged by size:
1621 674 427 194
PflMI CCAn_nnn'nTGG
Cuts at: 0 843 2916
Size: 843 2073
PfoI T'CCnGG_A
Cuts at: 0 615 791 2372 2916
Size: 615 176 1581 544
Fragments arranged by size:
1581 615 544 176
PmlI CAC'GTG
Cuts at: 0 2568 2916
Size: 2568 348
PshAI GACnn'nnGTC
Cuts at: 0 562 2916
Size: 562 2354
PsiI TTA'TAA
Cuts at: 0 1133 2916
Size: 1133 1783
PspOMI G'GGCC_C
Cuts at: 0 2328 2916
Size: 2328 588
PstI C_TGCA'G
Cuts at: 0 2520 2535 2916
Size: 2520 15 381
Fragments arranged by size:
2520 381 15
PvuII CAG'CTG
Cuts at: 0 649 2916
Size: 649 2267
SacI G_AGCT'C
Cuts at: 0 383 1904 2916
Size: 383 1521 1012
Fragments arranged by size:
1521 1012 383
SapI GCTCTTCn'nnn_
Cuts at: 0 2276 2916
Size: 2276 640
ScaI AGT'ACT
Cuts at: 0 193 2916
Size: 193 2723
SfcI C'TryA_G
Cuts at: 0 2516 2531 2916
Size: 2516 15 385
Fragments arranged by size:
2516 385 15
SfoI GGC'GCC
Cuts at: 0 1332 2916
Size: 1332 1584
SgrAI Cr'CCGG_yG
Cuts at: 0 1360 2916
Size: 1360 1556
SmaI CCC'GGG
Cuts at: 0 2791 2916
Size: 2791 125
SmlI C'TyrA_G
Cuts at: 0 2014 2074 2916
Size: 2014 60 842
Fragments arranged by size:
2014 842 60
SphI G_CATG'C
Cuts at: 0 736 2916
Size: 736 2180
StyI C'CwwG_G
Cuts at: 0 417 1856 2759 2916
Size: 417 1439 903 157
Fragments arranged by size:
1439 903 417 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2598 2686 2916
Size: 2598 88 230
Fragments arranged by size:
2598 230 88
TatI w'GTAC_w
Cuts at: 0 191 938 1442 1940 2603 2916
Size: 191 747 504 498 663 313
Fragments arranged by size:
747 663 504 498 313 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2232 2916
Size: 2232 684
XmaI C'CCGG_G
Cuts at: 0 2789 2916
Size: 2789 127
XmnI GAAnn'nnTTC
Cuts at: 0 2348 2916
Size: 2348 568
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AgeI AleI AlwNI
ApaI ApoI AseI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI Bsu36I BtgI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NarI NcoI NsiI NspI PasI PciI PflMI
PfoI PmlI PshAI PsiI PspOMI PstI PvuII SacI
SapI ScaI SfcI SfoI SgrAI SmaI SmlI SphI
StyI TaqII TatI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AhdI AlfI AloI ApaLI
AscI AsiSI AvrII BaeI BamHI BciVI BglI BmtI
BsaXI BsiEI BsiWI BsmI BspMI BssHII BssSI BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI EagI
EcoRV FalI FseI FspAI HpaI MluI NaeI NdeI
NgoMIV NheI NotI NruI PacI PmeI PpiI PpuMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SgfI SnaBI SpeI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI ZraI