Restriction map for pF1KSDA0033
 (Linear) (Six-base) MAPSORT of: KIAA0033.seq  Check: 4901  from: 1  to: 873



KIAA0033.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:10  ..

AccI GT'mk_AC

Cuts at:      0     408     524     873 
   Size:        408     116     349

  Fragments arranged by size: 

                 408     349     116
 
AhdI GACnn_n'nnGTC

Cuts at:      0      83     873 
   Size:         83     790
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     395     873 
   Size:        395     478
 
ApoI r'AATT_y

Cuts at:      0     219     865     873 
   Size:        219     646       8

  Fragments arranged by size: 

                 646     219       8
 
AseI AT'TA_AT

Cuts at:      0     644     873 
   Size:        644     229
 
BanI G'GyrC_C

Cuts at:      0      93     475     873 
   Size:         93     382     398

  Fragments arranged by size: 

                 398     382      93
 
BbeI G_GCGC'C

Cuts at:      0      97     873 
   Size:         97     776
 
BbvCI CC'TCA_GC

Cuts at:      0     227     873 
   Size:        227     646
 
BglI GCCn_nnn'nGGC

Cuts at:      0     736     873 
   Size:        736     137
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     604     873 
   Size:        604     269
 
Bpu10I CC'TnA_GC

Cuts at:      0     227     873 
   Size:        227     646
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      91     101     507     873 
   Size:         91      10     406     366

  Fragments arranged by size: 

                 406     366      91      10
 
BsaHI Gr'CG_yC

Cuts at:      0      94     873 
   Size:         94     779
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     764     794     873 
   Size:        764      30      79

  Fragments arranged by size: 

                 764      79      30
 
BsiEI CG_ry'CG

Cuts at:      0      28     873 
   Size:         28     845
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     555     873 
   Size:        555     318
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     273     873 
   Size:        273     600
 
BstZ17I GTA'TAC

Cuts at:      0     409     525     873 
   Size:        409     116     348

  Fragments arranged by size: 

                 409     348     116
 
BtgI C'CryG_G

Cuts at:      0      56     873 
   Size:         56     817
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     339     873 
   Size:        339     534
 
HaeII r_GCGC'y

Cuts at:      0      41      97     873 
   Size:         41      56     776

  Fragments arranged by size: 

                 776      56      41
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      69     101     873 
   Size:         69      32     772

  Fragments arranged by size: 

                 772      69      32
 
KasI G'GCGC_C

Cuts at:      0      93     873 
   Size:         93     780
 
MspA1I CmG'CkG

Cuts at:      0      73     873 
   Size:         73     800
 
NarI GG'CG_CC

Cuts at:      0      94     873 
   Size:         94     779
 
PfoI T'CCnGG_A

Cuts at:      0     784     873 
   Size:        784      89
 
PstI C_TGCA'G

Cuts at:      0     192     873 
   Size:        192     681
 
PvuI CG_AT'CG

Cuts at:      0      28     873 
   Size:         28     845
 
ScaI AGT'ACT

Cuts at:      0     338     873 
   Size:        338     535
 
SfcI C'TryA_G

Cuts at:      0     188     873 
   Size:        188     685
 
SfoI GGC'GCC

Cuts at:      0      95     873 
   Size:         95     778
 
SspI AAT'ATT

Cuts at:      0     797     873 
   Size:        797      76
 
StyI C'CwwG_G

Cuts at:      0     284     873 
   Size:        284     589
 
TatI w'GTAC_w

Cuts at:      0     336     873 
   Size:        336     537
 

 Enzymes that do cut:

     AccI     AhdI     AlfI     ApoI     AseI     BanI     BbeI    BbvCI
     BglI     BplI   Bpu10I     BsaI    BsaHI    BsaXI    BsiEI    BspMI
    BstXI  BstZ17I     BtgI     FalI    HaeII    Hin4I     KasI   MspA1I
     NarI     PfoI     PstI     PvuI     ScaI     SfcI     SfoI     SspI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI    BsaAI    BsaBI    BsaWI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstYI   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com