Restriction map for
pF1KA0034
(Linear) (Six-base) MAPSORT of: KIAA0034.seq Check: 1656 from: 1 to: 5025
KIAA0034.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 0 2430 5025
Size: 2430 2595
Acc65I G'GTAC_C
Cuts at: 0 868 5025
Size: 868 4157
AclI AA'CG_TT
Cuts at: 0 640 3300 5025
Size: 640 2660 1725
Fragments arranged by size:
2660 1725 640
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 247 1100 1228 1303 1400 1729 1748
Size: 63 184 853 128 75 97 329 19
Cuts at: 1748 2495 2975 3221 4670 4829 5025
Size: 747 480 246 1449 159 196
Fragments arranged by size:
1449 853 747 480 329 246 196 184
159 128 97 75 63 19
AflII C'TTAA_G
Cuts at: 0 2287 5025
Size: 2287 2738
AflIII A'CryG_T
Cuts at: 0 3112 3114 5025
Size: 3112 2 1911
Fragments arranged by size:
3112 1911 2
AleI CACnn'nnGTG
Cuts at: 0 3701 4298 5025
Size: 3701 597 727
Fragments arranged by size:
3701 727 597
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1703 2893 5025
Size: 1703 1190 2132
Fragments arranged by size:
2132 1703 1190
AlwNI CAG_nnn'CTG
Cuts at: 0 12 51 2034 2091 2971 4437 5025
Size: 12 39 1983 57 880 1466 588
Fragments arranged by size:
1983 1466 880 588 57 39 12
ApaLI G'TGCA_C
Cuts at: 0 2179 5025
Size: 2179 2846
ApoI r'AATT_y
Cuts at: 0 106 1099 1169 2280 2594 2808 3220 3562
Size: 106 993 70 1111 314 214 412 342
Cuts at: 3562 3793 3976 4237 5025
Size: 231 183 261 788
Fragments arranged by size:
1111 993 788 412 342 314 261 231
214 183 106 70
AseI AT'TA_AT
Cuts at: 0 2792 5025
Size: 2792 2233
AvaI C'yCGr_G
Cuts at: 0 3491 5025
Size: 3491 1534
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 39 72 1239 1272 5025
Size: 39 33 1167 33 3753
Fragments arranged by size:
3753 1167 39 33 33
BamHI G'GATC_C
Cuts at: 0 2146 2853 5025
Size: 2146 707 2172
Fragments arranged by size:
2172 2146 707
BanI G'GyrC_C
Cuts at: 0 449 868 4956 5025
Size: 449 419 4088 69
Fragments arranged by size:
4088 449 419 69
BanII G_rGCy'C
Cuts at: 0 1833 3936 5025
Size: 1833 2103 1089
Fragments arranged by size:
2103 1833 1089
BbsI GAAGACnn'nnnn_
Cuts at: 0 195 741 1988 5025
Size: 195 546 1247 3037
Fragments arranged by size:
3037 1247 546 195
BbvCI CC'TCA_GC
Cuts at: 0 2540 5025
Size: 2540 2485
BciVI GTATCCnnnnn_n'
Cuts at: 0 1267 1519 5025
Size: 1267 252 3506
Fragments arranged by size:
3506 1267 252
BclI T'GATC_A
Cuts at: 0 3896 5025
Size: 3896 1129
BfrBI ATG'CAT
Cuts at: 0 1770 5025
Size: 1770 3255
BlpI GC'TnA_GC
Cuts at: 0 4487 5025
Size: 4487 538
Bme1580I G_kGCm'C
Cuts at: 0 2183 5025
Size: 2183 2842
BmrI ACTGGGnnnn_n'
Cuts at: 0 1396 2222 3336 3456 3928 4905 4928 5025
Size: 1396 826 1114 120 472 977 23 97
Fragments arranged by size:
1396 1114 977 826 472 120 97 23
BmtI G_CTAG'C
Cuts at: 0 230 599 5025
Size: 230 369 4426
Fragments arranged by size:
4426 369 230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 28 408 692 743 1166 1517 3043
Size: 22 6 380 284 51 423 351 1526
Cuts at: 3043 3342 4027 5025
Size: 299 685 998
Fragments arranged by size:
1526 998 685 423 380 351 299 284
51 22 6
Bpu10I CC'TnA_GC
Cuts at: 0 2267 2540 2634 5025
Size: 2267 273 94 2391
Fragments arranged by size:
2391 2267 273 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1763 1774 3946 5025
Size: 880 883 11 2172 1079
Fragments arranged by size:
2172 1079 883 880 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 2134 2895 5025
Size: 2134 761 2130
Fragments arranged by size:
2134 2130 761
BsaAI yAC'GTr
Cuts at: 0 1060 3115 5025
Size: 1060 2055 1910
Fragments arranged by size:
2055 1910 1060
BsaBI GATnn'nnATC
Cuts at: 0 2741 5025
Size: 2741 2284
BsaWI w'CCGG_w
Cuts at: 0 3215 5025
Size: 3215 1810
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1336 1366 5025
Size: 1336 30 3659
Fragments arranged by size:
3659 1336 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1226 2626 5025
Size: 1226 1400 2399
Fragments arranged by size:
2399 1400 1226
BseYI C'CCAG_C
Cuts at: 0 63 250 1212 1217 5025
Size: 63 187 962 5 3808
Fragments arranged by size:
3808 962 187 63 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 497 902 1934 4322 5025
Size: 497 405 1032 2388 703
Fragments arranged by size:
2388 1032 703 497 405
BsiHKAI G_wGCw'C
Cuts at: 0 2183 2366 3936 4004 5025
Size: 2183 183 1570 68 1021
Fragments arranged by size:
2183 1570 1021 183 68
BsmI GAATG_Cn'
Cuts at: 0 1690 5025
Size: 1690 3335
Bsp1286I G_dGCh'C
Cuts at: 0 1833 2183 2366 3936 4004 5025
Size: 1833 350 183 1570 68 1021
Fragments arranged by size:
1833 1570 1021 350 183 68
BspEI T'CCGG_A
Cuts at: 0 3215 5025
Size: 3215 1810
BspHI T'CATG_A
Cuts at: 0 215 924 2067 2598 2984 4805 5025
Size: 215 709 1143 531 386 1821 220
Fragments arranged by size:
1821 1143 709 531 386 220 215
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 880 3321 5025
Size: 880 2441 1704
Fragments arranged by size:
2441 1704 880
BsrDI GCAATG_nn'
Cuts at: 0 794 2808 3189 3312 4616 5025
Size: 794 2014 381 123 1304 409
Fragments arranged by size:
2014 1304 794 409 381 123
BsrFI r'CCGG_y
Cuts at: 0 1072 5025
Size: 1072 3953
BsrGI T'GTAC_A
Cuts at: 0 2409 2922 3650 3831 5025
Size: 2409 513 728 181 1194
Fragments arranged by size:
2409 1194 728 513 181
BssSI C'ACGA_G
Cuts at: 0 4298 5025
Size: 4298 727
BstBI TT'CG_AA
Cuts at: 0 186 5025
Size: 186 4839
BstEII G'GTnAC_C
Cuts at: 0 3604 5025
Size: 3604 1421
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2864 3601 5025
Size: 2864 737 1424
Fragments arranged by size:
2864 1424 737
BstYI r'GATC_y
Cuts at: 0 338 2146 2736 2853 5025
Size: 338 1808 590 117 2172
Fragments arranged by size:
2172 1808 590 338 117
Bsu36I CC'TnA_GG
Cuts at: 0 1438 5025
Size: 1438 3587
BtgI C'CryG_G
Cuts at: 0 4282 5025
Size: 4282 743
BtsI GCAGTG_nn'
Cuts at: 0 746 1687 1922 3089 4669 5025
Size: 746 941 235 1167 1580 356
Fragments arranged by size:
1580 1167 941 746 356 235
ClaI AT'CG_AT
Cuts at: 0 2337 5025
Size: 2337 2688
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3420 3455 5025
Size: 3420 35 1570
Fragments arranged by size:
3420 1570 35
DraI TTT'AAA
Cuts at: 0 2187 5025
Size: 2187 2838
DraIII CAC_nnn'GTG
Cuts at: 0 1429 5025
Size: 1429 3596
DrdI GACnn_nn'nnGTC
Cuts at: 0 96 5025
Size: 96 4929
EaeI y'GGCC_r
Cuts at: 0 2769 5025
Size: 2769 2256
EarI CTCTTCn'nnn_
Cuts at: 0 266 1613 2725 2821 3884 4546 5025
Size: 266 1347 1112 96 1063 662 479
Fragments arranged by size:
1347 1112 1063 662 479 266 96
EcoICRI GAG'CTC
Cuts at: 0 3934 5025
Size: 3934 1091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 28 63 247 408 692 743 1100
Size: 22 6 35 184 161 284 51 357
Cuts at: 1100 1166 1228 1303 1400 1517 1729 1748 2495
Size: 66 62 75 97 117 212 19 747
Cuts at: 2495 2975 3043 3221 3342 4027 4670 4829 5025
Size: 480 68 178 121 685 643 159 196
Fragments arranged by size:
747 685 643 480 357 284 212 196
184 178 161 159 121 117 97 75
68 66 62 51 35 22 19 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4600 5025
Size: 4600 425
EcoO109I rG'GnC_Cy
Cuts at: 0 1732 2169 2949 4930 5025
Size: 1732 437 780 1981 95
Fragments arranged by size:
1981 1732 780 437 95
EcoRI G'AATT_C
Cuts at: 0 1169 2594 2808 3793 4237 5025
Size: 1169 1425 214 985 444 788
Fragments arranged by size:
1425 1169 985 788 444 214
EcoRV GAT'ATC
Cuts at: 0 4179 5025
Size: 4179 846
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1642 2909 4084 4612 4833 5025
Size: 1642 1267 1175 528 221 192
Fragments arranged by size:
1642 1267 1175 528 221 192
HaeII r_GCGC'y
Cuts at: 0 212 5025
Size: 212 4813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3388 3420 4125 4157 4462 4494 5025
Size: 3388 32 705 32 305 32 531
Fragments arranged by size:
3388 705 531 305 32 32 32
HincII GTy'rAC
Cuts at: 0 1956 2081 2157 2431 2466 3328 5025
Size: 1956 125 76 274 35 862 1697
Fragments arranged by size:
1956 1697 862 274 125 76 35
HpaI GTT'AAC
Cuts at: 0 2157 5025
Size: 2157 2868
KpnI G_GTAC'C
Cuts at: 0 872 5025
Size: 872 4153
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1503 2694 3618 3887 4168 4268 5025
Size: 1503 1191 924 269 281 100 757
Fragments arranged by size:
1503 1191 924 757 281 269 100
MscI TGG'CCA
Cuts at: 0 2771 5025
Size: 2771 2254
MslI CAynn'nnrTG
Cuts at: 0 160 280 851 880 3701 3839 3943 4298
Size: 160 120 571 29 2821 138 104 355
Cuts at: 4298 5025
Size: 727
Fragments arranged by size:
2821 727 571 355 160 138 120 104
29
MspA1I CmG'CkG
Cuts at: 0 937 3718 5025
Size: 937 2781 1307
Fragments arranged by size:
2781 1307 937
NdeI CA'TA_TG
Cuts at: 0 3285 5025
Size: 3285 1740
NheI G'CTAG_C
Cuts at: 0 226 595 5025
Size: 226 369 4430
Fragments arranged by size:
4430 369 226
NsiI A_TGCA'T
Cuts at: 0 1772 5025
Size: 1772 3253
NspI r_CATG'y
Cuts at: 0 592 1774 3838 5025
Size: 592 1182 2064 1187
Fragments arranged by size:
2064 1187 1182 592
PasI CC'CwG_GG
Cuts at: 0 1119 5025
Size: 1119 3906
PflMI CCAn_nnn'nTGG
Cuts at: 0 51 2358 4990 5005 5025
Size: 51 2307 2632 15 20
Fragments arranged by size:
2632 2307 51 20 15
PmlI CAC'GTG
Cuts at: 0 3115 5025
Size: 3115 1910
PpuMI rG'GwC_Cy
Cuts at: 0 1732 2169 2949 4930 5025
Size: 1732 437 780 1981 95
Fragments arranged by size:
1981 1732 780 437 95
PstI C_TGCA'G
Cuts at: 0 1871 2039 2994 4056 4664 5025
Size: 1871 168 955 1062 608 361
Fragments arranged by size:
1871 1062 955 608 361 168
PvuII CAG'CTG
Cuts at: 0 937 3718 5025
Size: 937 2781 1307
Fragments arranged by size:
2781 1307 937
SacI G_AGCT'C
Cuts at: 0 3936 5025
Size: 3936 1089
SalI G'TCGA_C
Cuts at: 0 2429 5025
Size: 2429 2596
SapI GCTCTTCn'nnn_
Cuts at: 0 1613 3884 5025
Size: 1613 2271 1141
Fragments arranged by size:
2271 1613 1141
ScaI AGT'ACT
Cuts at: 0 1249 3466 3747 4816 5025
Size: 1249 2217 281 1069 209
Fragments arranged by size:
2217 1249 1069 281 209
SexAI A'CCwGG_T
Cuts at: 0 2837 2915 5025
Size: 2837 78 2110
Fragments arranged by size:
2837 2110 78
SfcI C'TryA_G
Cuts at: 0 554 710 1867 2035 2241 2438 2990 4052
Size: 554 156 1157 168 206 197 552 1062
Cuts at: 4052 4212 4660 4877 5025
Size: 160 448 217 148
Fragments arranged by size:
1157 1062 554 552 448 217 206 197
168 160 156 148
SmlI C'TyrA_G
Cuts at: 0 859 1753 1778 2287 3491 3925 5025
Size: 859 894 25 509 1204 434 1100
Fragments arranged by size:
1204 1100 894 859 509 434 25
SnaBI TAC'GTA
Cuts at: 0 1060 5025
Size: 1060 3965
SpeI A'CTAG_T
Cuts at: 0 3436 5025
Size: 3436 1589
SphI G_CATG'C
Cuts at: 0 1774 5025
Size: 1774 3251
SspI AAT'ATT
Cuts at: 0 4032 5025
Size: 4032 993
StuI AGG'CCT
Cuts at: 0 1311 5025
Size: 1311 3714
StyI C'CwwG_G
Cuts at: 0 2578 4037 4187 5025
Size: 2578 1459 150 838
Fragments arranged by size:
2578 1459 838 150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1378 1942 3691 5025
Size: 1378 564 1749 1334
Fragments arranged by size:
1749 1378 1334 564
TatI w'GTAC_w
Cuts at: 0 1247 1314 1684 2409 2922 3464 3650 3745
Size: 1247 67 370 725 513 542 186 95
Cuts at: 3745 3831 4814 5025
Size: 86 983 211
Fragments arranged by size:
1247 983 725 542 513 370 211 186
95 86 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4554 4586 5025
Size: 4554 32 439
Fragments arranged by size:
4554 439 32
Tth111I GACn'n_nGTC
Cuts at: 0 2347 5025
Size: 2347 2678
XbaI T'CTAG_A
Cuts at: 0 4021 5025
Size: 4021 1004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4151 4960 5025
Size: 4151 809 65
Fragments arranged by size:
4151 809 65
XhoI C'TCGA_G
Cuts at: 0 3491 5025
Size: 3491 1534
XmnI GAAnn'nnTTC
Cuts at: 0 3419 3793 5025
Size: 3419 374 1232
Fragments arranged by size:
3419 1232 374
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AlfI
AlwNI ApaLI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BciVI BclI BfrBI BlpI Bme1580I
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HpaI
KpnI MmeI MscI MslI MspA1I NdeI NheI NsiI
NspI PasI PflMI PmlI PpuMI PstI PvuII SacI
SalI SapI ScaI SexAI SfcI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AloI ApaI AscI
AsiSI AvrII BbeI BcgI BglI BglII BmgBI BplI
BsaHI BsiEI BsiWI BsmBI BsrBI BssHII BstAPI BstZ17I
BtgZI EagI EciI FseI FspI FspAI HindIII KasI
MfeI MluI NaeI NarI NcoI NgoMIV NotI NruI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SanDI SbfI SfiI
SfoI SgfI SgrAI SmaI SrfI SwaI XmaI ZraI