Restriction map for
pF1KSDA0036
(Linear) (Six-base) MAPSORT of: KIAA0036.seq Check: 355 from: 1 to: 1794
KIAA0036.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AatII G_ACGT'C
Cuts at: 0 777 1794
Size: 777 1017
AccI GT'mk_AC
Cuts at: 0 777 1794
Size: 777 1017
AclI AA'CG_TT
Cuts at: 0 1550 1794
Size: 1550 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 416 1393 1600 1690 1794
Size: 65 351 977 207 90 104
Fragments arranged by size:
977 351 207 104 90 65
AfeI AGC'GCT
Cuts at: 0 1280 1794
Size: 1280 514
AflIII A'CryG_T
Cuts at: 0 370 1794
Size: 370 1424
AleI CACnn'nnGTG
Cuts at: 0 1689 1794
Size: 1689 105
ApaI G_GGCC'C
Cuts at: 0 1494 1794
Size: 1494 300
ApoI r'AATT_y
Cuts at: 0 304 339 728 805 1215 1285 1764 1794
Size: 304 35 389 77 410 70 479 30
Fragments arranged by size:
479 410 389 304 77 70 35 30
AvaI C'yCGr_G
Cuts at: 0 1088 1794
Size: 1088 706
BanI G'GyrC_C
Cuts at: 0 1569 1794
Size: 1569 225
BanII G_rGCy'C
Cuts at: 0 1414 1445 1494 1509 1610 1794
Size: 1414 31 49 15 101 184
Fragments arranged by size:
1414 184 101 49 31 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 1407 1794
Size: 1407 387
BclI T'GATC_A
Cuts at: 0 498 1794
Size: 498 1296
BglI GCCn_nnn'nGGC
Cuts at: 0 1651 1794
Size: 1651 143
Bme1580I G_kGCm'C
Cuts at: 0 1494 1794
Size: 1494 300
BmrI ACTGGGnnnn_n'
Cuts at: 0 806 1794
Size: 806 988
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1253 1794
Size: 1253 541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1736 1794
Size: 1030 706 58
Fragments arranged by size:
1030 706 58
Bpu10I CC'TnA_GC
Cuts at: 0 62 389 1604 1794
Size: 62 327 1215 190
Fragments arranged by size:
1215 327 190 62
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1442 1794
Size: 1442 352
BsaHI Gr'CG_yC
Cuts at: 0 774 1794
Size: 774 1020
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 1794
Size: 452 30 1312
Fragments arranged by size:
1312 452 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 990 1794
Size: 990 804
BseYI C'CCAG_C
Cuts at: 0 242 1508 1794
Size: 242 1266 286
Fragments arranged by size:
1266 286 242
BsiHKAI G_wGCw'C
Cuts at: 0 1445 1610 1794
Size: 1445 165 184
Fragments arranged by size:
1445 184 165
Bsp1286I G_dGCh'C
Cuts at: 0 1414 1445 1494 1509 1610 1794
Size: 1414 31 49 15 101 184
Fragments arranged by size:
1414 184 101 49 31 15
BspHI T'CATG_A
Cuts at: 0 551 1794
Size: 551 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 763 1794
Size: 763 1031
BsrDI GCAATG_nn'
Cuts at: 0 264 1794
Size: 264 1530
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1632 1794
Size: 1632 162
BstYI r'GATC_y
Cuts at: 0 26 541 985 1621 1794
Size: 26 515 444 636 173
Fragments arranged by size:
636 515 444 173 26
DrdI GACnn_nn'nnGTC
Cuts at: 0 773 1794
Size: 773 1021
EarI CTCTTCn'nnn_
Cuts at: 0 451 863 1028 1154 1493 1615 1794
Size: 451 412 165 126 339 122 179
Fragments arranged by size:
451 412 339 179 165 126 122
EcoICRI GAG'CTC
Cuts at: 0 1443 1608 1794
Size: 1443 165 186
Fragments arranged by size:
1443 186 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 416 1030 1393 1600 1690 1736 1794
Size: 65 351 614 363 207 90 46 58
Fragments arranged by size:
614 363 351 207 90 65 58 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1331 1794
Size: 1331 463
EcoO109I rG'GnC_Cy
Cuts at: 0 1038 1490 1491 1794
Size: 1038 452 1 303
Fragments arranged by size:
1038 452 303 1
EcoRI G'AATT_C
Cuts at: 0 805 1794
Size: 805 989
HaeII r_GCGC'y
Cuts at: 0 1282 1794
Size: 1282 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 426 458 533 565 1794
Size: 426 32 75 32 1229
Fragments arranged by size:
1229 426 75 32 32
HindIII A'AGCT_T
Cuts at: 0 649 946 1699 1794
Size: 649 297 753 95
Fragments arranged by size:
753 649 297 95
MfeI C'AATT_G
Cuts at: 0 1051 1794
Size: 1051 743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 1794
Size: 208 1586
MslI CAynn'nnrTG
Cuts at: 0 269 556 1689 1794
Size: 269 287 1133 105
Fragments arranged by size:
1133 287 269 105
MspA1I CmG'CkG
Cuts at: 0 331 1101 1258 1794
Size: 331 770 157 536
Fragments arranged by size:
770 536 331 157
NspI r_CATG'y
Cuts at: 0 374 896 1794
Size: 374 522 898
Fragments arranged by size:
898 522 374
PciI A'CATG_T
Cuts at: 0 370 1794
Size: 370 1424
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1592 1624 1794
Size: 1592 32 170
Fragments arranged by size:
1592 170 32
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1794
Size: 1038 756
PsiI TTA'TAA
Cuts at: 0 676 1794
Size: 676 1118
PspOMI G'GGCC_C
Cuts at: 0 1490 1794
Size: 1490 304
PstI C_TGCA'G
Cuts at: 0 336 549 1794
Size: 336 213 1245
Fragments arranged by size:
1245 336 213
PvuII CAG'CTG
Cuts at: 0 331 1101 1258 1794
Size: 331 770 157 536
Fragments arranged by size:
770 536 331 157
SacI G_AGCT'C
Cuts at: 0 1445 1610 1794
Size: 1445 165 184
Fragments arranged by size:
1445 184 165
SapI GCTCTTCn'nnn_
Cuts at: 0 863 1493 1615 1794
Size: 863 630 122 179
Fragments arranged by size:
863 630 179 122
ScaI AGT'ACT
Cuts at: 0 684 1794
Size: 684 1110
SfcI C'TryA_G
Cuts at: 0 35 332 545 562 1794
Size: 35 297 213 17 1232
Fragments arranged by size:
1232 297 213 35 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1651 1794
Size: 1651 143
SmlI C'TyrA_G
Cuts at: 0 1457 1794
Size: 1457 337
SphI G_CATG'C
Cuts at: 0 896 1794
Size: 896 898
SspI AAT'ATT
Cuts at: 0 181 1794
Size: 181 1613
StuI AGG'CCT
Cuts at: 0 1629 1794
Size: 1629 165
StyI C'CwwG_G
Cuts at: 0 22 1327 1640 1794
Size: 22 1305 313 154
Fragments arranged by size:
1305 313 154 22
TatI w'GTAC_w
Cuts at: 0 486 682 1794
Size: 486 196 1112
Fragments arranged by size:
1112 486 196
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1163 1195 1794
Size: 1163 32 599
Fragments arranged by size:
1163 599 32
Tth111I GACn'n_nGTC
Cuts at: 0 529 1393 1794
Size: 529 864 401
Fragments arranged by size:
864 529 401
XmnI GAAnn'nnTTC
Cuts at: 0 320 341 476 1118 1794
Size: 320 21 135 642 676
Fragments arranged by size:
676 642 320 135 21
ZraI GAC'GTC
Cuts at: 0 775 1794
Size: 775 1019
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflIII AleI ApaI
ApoI AvaI BanI BanII BbsI BclI BglI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI
BseYI BsiHKAI Bsp1286I BspHI BspMI BsrDI BstXI BstYI
DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII
Hin4I HindIII MfeI MmeI MslI MspA1I NspI PciI
PpiI PpuMI PsiI PspOMI PstI PvuII SacI SapI
ScaI SfcI SfiI SmlI SphI SspI StuI StyI
TatI TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AflII AgeI AhdI AlfI AloI AlwNI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BglII BlpI BmgBI BmtI
BsaI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII EaeI EagI EciI EcoRV FalI
FseI FspI FspAI HincII HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SwaI TaqII XbaI XcmI XhoI XmaI