Restriction map for
pF1KA0038
(Linear) (Six-base) MAPSORT of: KIAA0038.seq Check: 873 from: 1 to: 684
KIAA0038.orf
With 182 enzymes: SgfI *
July 18, 2007 13:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 474 684
Size: 396 78 210
Fragments arranged by size:
396 210 78
AflII C'TTAA_G
Cuts at: 0 283 684
Size: 283 401
ApaLI G'TGCA_C
Cuts at: 0 308 684
Size: 308 376
ApoI r'AATT_y
Cuts at: 0 238 262 684
Size: 238 24 422
Fragments arranged by size:
422 238 24
BamHI G'GATC_C
Cuts at: 0 514 684
Size: 514 170
BanI G'GyrC_C
Cuts at: 0 643 684
Size: 643 41
BanII G_rGCy'C
Cuts at: 0 119 684
Size: 119 565
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 157 191 684
Size: 157 34 493
Fragments arranged by size:
493 157 34
Bme1580I G_kGCm'C
Cuts at: 0 312 684
Size: 312 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 684
Size: 556 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 555 684
Size: 555 129
BsaAI yAC'GTr
Cuts at: 0 135 684
Size: 135 549
BsaHI Gr'CG_yC
Cuts at: 0 598 684
Size: 598 86
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 554 584 684
Size: 554 30 100
Fragments arranged by size:
554 100 30
BsiEI CG_ry'CG
Cuts at: 0 27 324 459 684
Size: 27 297 135 225
Fragments arranged by size:
297 225 135 27
BsiHKAI G_wGCw'C
Cuts at: 0 312 558 684
Size: 312 246 126
Fragments arranged by size:
312 246 126
Bsp1286I G_dGCh'C
Cuts at: 0 119 312 558 684
Size: 119 193 246 126
Fragments arranged by size:
246 193 126 119
BsrDI GCAATG_nn'
Cuts at: 0 341 684
Size: 341 343
BsrFI r'CCGG_y
Cuts at: 0 458 684
Size: 458 226
BstYI r'GATC_y
Cuts at: 0 189 514 684
Size: 189 325 170
Fragments arranged by size:
325 189 170
BtgI C'CryG_G
Cuts at: 0 63 81 476 510 684
Size: 63 18 395 34 174
Fragments arranged by size:
395 174 63 34 18
BtsI GCAGTG_nn'
Cuts at: 0 75 684
Size: 75 609
DraI TTT'AAA
Cuts at: 0 243 684
Size: 243 441
DraIII CAC_nnn'GTG
Cuts at: 0 334 684
Size: 334 350
EaeI y'GGCC_r
Cuts at: 0 78 684
Size: 78 606
EarI CTCTTCn'nnn_
Cuts at: 0 539 684
Size: 539 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 684
Size: 19 665
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 474 556 684
Size: 396 78 82 128
Fragments arranged by size:
396 128 82 78
EcoO109I rG'GnC_Cy
Cuts at: 0 59 469 684
Size: 59 410 215
Fragments arranged by size:
410 215 59
EcoRI G'AATT_C
Cuts at: 0 262 684
Size: 262 422
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 309 684
Size: 309 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 554 586 647 679 684
Size: 554 32 61 32 5
Fragments arranged by size:
554 61 32 32 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 684
Size: 542 142
MscI TGG'CCA
Cuts at: 0 80 684
Size: 80 604
MspA1I CmG'CkG
Cuts at: 0 65 684
Size: 65 619
NcoI C'CATG_G
Cuts at: 0 81 476 684
Size: 81 395 208
Fragments arranged by size:
395 208 81
NruI TCG'CGA
Cuts at: 0 595 684
Size: 595 89
PpuMI rG'GwC_Cy
Cuts at: 0 59 684
Size: 59 625
PvuI CG_AT'CG
Cuts at: 0 27 324 684
Size: 27 297 360
Fragments arranged by size:
360 297 27
SacII CC_GC'GG
Cuts at: 0 66 684
Size: 66 618
SanDI GG'GwC_CC
Cuts at: 0 59 684
Size: 59 625
SfcI C'TryA_G
Cuts at: 0 33 684
Size: 33 651
SmlI C'TyrA_G
Cuts at: 0 283 684
Size: 283 401
SnaBI TAC'GTA
Cuts at: 0 135 684
Size: 135 549
StuI AGG'CCT
Cuts at: 0 651 684
Size: 651 33
StyI C'CwwG_G
Cuts at: 0 81 476 684
Size: 81 395 208
Fragments arranged by size:
395 208 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 314 684
Size: 314 370
XmnI GAAnn'nnTTC
Cuts at: 0 665 684
Size: 665 19
Enzymes that do cut:
AcuI AflII ApaLI ApoI BamHI BanI BanII BcgI
Bme1580I BpmI BsaI BsaAI BsaHI BsaXI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BstYI BtgI BtsI DraI DraIII
EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI Hin4I
MmeI MscI MspA1I NcoI NruI PpuMI PvuI SacII
SanDI SfcI SmlI SnaBI StuI StyI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbsI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaBI BsaWI BseRI BseYI BsgI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI