Restriction map for
pF1KSDA0039
(Linear) (Six-base) MAPSORT of: KIAA0039.seq Check: 2229 from: 1 to: 1398
KIAA0039.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 546 1398
Size: 546 852
AccI GT'mk_AC
Cuts at: 0 1211 1398
Size: 1211 187
AflIII A'CryG_T
Cuts at: 0 482 1152 1398
Size: 482 670 246
Fragments arranged by size:
670 482 246
AlwNI CAG_nnn'CTG
Cuts at: 0 120 805 868 1398
Size: 120 685 63 530
Fragments arranged by size:
685 530 120 63
ApaI G_GGCC'C
Cuts at: 0 1327 1398
Size: 1327 71
ApoI r'AATT_y
Cuts at: 0 153 391 736 1398
Size: 153 238 345 662
Fragments arranged by size:
662 345 238 153
BanI G'GyrC_C
Cuts at: 0 681 1398
Size: 681 717
BanII G_rGCy'C
Cuts at: 0 165 432 1123 1327 1398
Size: 165 267 691 204 71
Fragments arranged by size:
691 267 204 165 71
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 1264 1398
Size: 443 821 134
Fragments arranged by size:
821 443 134
BfrBI ATG'CAT
Cuts at: 0 661 1398
Size: 661 737
BlpI GC'TnA_GC
Cuts at: 0 76 1398
Size: 76 1322
Bme1580I G_kGCm'C
Cuts at: 0 1327 1398
Size: 1327 71
BmtI G_CTAG'C
Cuts at: 0 277 1398
Size: 277 1121
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1107 1398
Size: 1107 291
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 975 1046 1049 1258 1398
Size: 975 71 3 209 140
Fragments arranged by size:
975 209 140 71 3
BseYI C'CCAG_C
Cuts at: 0 557 1398
Size: 557 841
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 1212 1398
Size: 431 781 186
Fragments arranged by size:
781 431 186
BsiHKAI G_wGCw'C
Cuts at: 0 432 1123 1398
Size: 432 691 275
Fragments arranged by size:
691 432 275
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1070 1398
Size: 1070 328
Bsp1286I G_dGCh'C
Cuts at: 0 165 432 1123 1327 1398
Size: 165 267 691 204 71
Fragments arranged by size:
691 267 204 165 71
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 540 546 1398
Size: 540 6 852
Fragments arranged by size:
852 540 6
BsrGI T'GTAC_A
Cuts at: 0 299 1398
Size: 299 1099
BstAPI GCAn_nnn'nTGC
Cuts at: 0 832 1398
Size: 832 566
BtsI GCAGTG_nn'
Cuts at: 0 266 773 999 1398
Size: 266 507 226 399
Fragments arranged by size:
507 399 266 226
DraI TTT'AAA
Cuts at: 0 741 1398
Size: 741 657
DrdI GACnn_nn'nnGTC
Cuts at: 0 37 1398
Size: 37 1361
EarI CTCTTCn'nnn_
Cuts at: 0 450 1235 1398
Size: 450 785 163
Fragments arranged by size:
785 450 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1398
Size: 19 1379
EcoICRI GAG'CTC
Cuts at: 0 430 1121 1398
Size: 430 691 277
Fragments arranged by size:
691 430 277
EcoO109I rG'GnC_Cy
Cuts at: 0 1323 1324 1398
Size: 1323 1 74
Fragments arranged by size:
1323 74 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1264 1398
Size: 1264 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1207 1239 1329 1361 1398
Size: 1030 32 145 32 90 32 37
Fragments arranged by size:
1030 145 90 37 32 32 32
HincII GTy'rAC
Cuts at: 0 102 1398
Size: 102 1296
HpaI GTT'AAC
Cuts at: 0 102 1398
Size: 102 1296
MluI A'CGCG_T
Cuts at: 0 1152 1398
Size: 1152 246
MslI CAynn'nnrTG
Cuts at: 0 69 295 1291 1398
Size: 69 226 996 107
Fragments arranged by size:
996 226 107 69
MspA1I CmG'CkG
Cuts at: 0 415 1398
Size: 415 983
NheI G'CTAG_C
Cuts at: 0 273 1398
Size: 273 1125
NsiI A_TGCA'T
Cuts at: 0 663 1398
Size: 663 735
NspI r_CATG'y
Cuts at: 0 175 486 1398
Size: 175 311 912
Fragments arranged by size:
912 311 175
PciI A'CATG_T
Cuts at: 0 482 1398
Size: 482 916
PshAI GACnn'nnGTC
Cuts at: 0 1368 1398
Size: 1368 30
PspOMI G'GGCC_C
Cuts at: 0 1323 1398
Size: 1323 75
PstI C_TGCA'G
Cuts at: 0 388 669 837 884 1398
Size: 388 281 168 47 514
Fragments arranged by size:
514 388 281 168 47
PvuII CAG'CTG
Cuts at: 0 415 1398
Size: 415 983
SacI G_AGCT'C
Cuts at: 0 432 1123 1398
Size: 432 691 275
Fragments arranged by size:
691 432 275
SbfI CC_TGCA'GG
Cuts at: 0 837 1398
Size: 837 561
SfcI C'TryA_G
Cuts at: 0 384 665 797 833 880 1398
Size: 384 281 132 36 47 518
Fragments arranged by size:
518 384 281 132 47 36
SmlI C'TyrA_G
Cuts at: 0 1122 1398
Size: 1122 276
StuI AGG'CCT
Cuts at: 0 839 1398
Size: 839 559
TatI w'GTAC_w
Cuts at: 0 299 1269 1398
Size: 299 970 129
Fragments arranged by size:
970 299 129
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1398
Size: 573 825
XmnI GAAnn'nnTTC
Cuts at: 0 1007 1398
Size: 1007 391
Enzymes that do cut:
AarI AccI AflIII AlwNI ApaI ApoI BanI BanII
BbsI BfrBI BlpI Bme1580I BmtI BpuEI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BstAPI BtsI
DraI DrdI EarI EciI EcoICRI EcoO109I FalI Hin4I
HincII HpaI MluI MslI MspA1I NheI NsiI NspI
PciI PshAI PspOMI PstI PvuII SacI SbfI SfcI
SmlI StuI TatI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BglI BglII BmgBI BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmI
BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraIII EaeI EagI Eco57MI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII KasI KpnI MfeI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NotI
NruI PacI PasI PflMI PfoI PmeI PmlI PpiI
PpuMI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TstI Tth111I XbaI XhoI XmaI ZraI