Restriction map for pF1KA0040
 (Linear) (Six-base) MAPSORT of: KIAA0040.seq  Check: 696  from: 1  to: 459



KIAA0040.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:13  ..

AhdI GACnn_n'nnGTC

Cuts at:      0      59     459 
   Size:         59     400
 
ApaLI G'TGCA_C

Cuts at:      0     247     459 
   Size:        247     212
 
ApoI r'AATT_y

Cuts at:      0     276     459 
   Size:        276     183
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      99     459 
   Size:         99     360
 
Bme1580I G_kGCm'C

Cuts at:      0     251     459 
   Size:        251     208
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      59     459 
   Size:         59     400
 
BsaAI yAC'GTr

Cuts at:      0      91     258     294     459 
   Size:         91     167      36     165

  Fragments arranged by size: 

                 167     165      91      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     459 
   Size:        364      95
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     459 
   Size:        251     208
 
Bsp1286I G_dGCh'C

Cuts at:      0     251     459 
   Size:        251     208
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     278     459 
   Size:        278     181
 
BsrDI GCAATG_nn'

Cuts at:      0     143     459 
   Size:        143     316
 
EcoO109I rG'GnC_Cy

Cuts at:      0     436     459 
   Size:        436      23
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      45      77     459 
   Size:         45      32     382

  Fragments arranged by size: 

                 382      45      32
 
MslI CAynn'nnrTG

Cuts at:      0     373     459 
   Size:        373      86
 
NspI r_CATG'y

Cuts at:      0     165     196     255     459 
   Size:        165      31      59     204

  Fragments arranged by size: 

                 204     165      59      31
 
PasI CC'CwG_GG

Cuts at:      0      81     459 
   Size:         81     378
 
PmlI CAC'GTG

Cuts at:      0      91     459 
   Size:         91     368
 
PstI C_TGCA'G

Cuts at:      0     153     459 
   Size:        153     306
 
SfcI C'TryA_G

Cuts at:      0     149     362     459 
   Size:        149     213      97

  Fragments arranged by size: 

                 213     149      97
 
SphI G_CATG'C

Cuts at:      0     165     459 
   Size:        165     294
 
StyI C'CwwG_G

Cuts at:      0      37     459 
   Size:         37     422
 
TatI w'GTAC_w

Cuts at:      0     335     352     459 
   Size:        335      17     107

  Fragments arranged by size: 

                 335     107      17
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      88     459 
   Size:         88     371
 

 Enzymes that do cut:

     AhdI    ApaLI     ApoI    BciVI Bme1580I     BmrI    BsaAI     BsgI
  BsiHKAI Bsp1286I    BspMI    BsrDI EcoO109I    Hin4I     MslI     NspI
     PasI     PmlI     PstI     SfcI     SphI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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