Restriction map for
pF1KA0041
(Linear) (Six-base) MAPSORT of: KIAA0041.seq Check: 9965 from: 1 to: 2334
KIAA0041.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 52 310 508 1189 1622 1784 1807
Size: 33 19 258 198 681 433 162 23
Cuts at: 1807 2334
Size: 527
Fragments arranged by size:
681 527 433 258 198 162 33 23
19
AflIII A'CryG_T
Cuts at: 0 1966 2334
Size: 1966 368
AlwNI CAG_nnn'CTG
Cuts at: 0 860 2334
Size: 860 1474
ApaLI G'TGCA_C
Cuts at: 0 662 2334
Size: 662 1672
ApoI r'AATT_y
Cuts at: 0 273 355 901 1316 1531 1627 1691 2329
Size: 273 82 546 415 215 96 64 638
Cuts at: 2329 2334
Size: 5
Fragments arranged by size:
638 546 415 273 215 96 82 64
5
AseI AT'TA_AT
Cuts at: 0 530 2334
Size: 530 1804
BanI G'GyrC_C
Cuts at: 0 2089 2334
Size: 2089 245
BanII G_rGCy'C
Cuts at: 0 1705 1957 2334
Size: 1705 252 377
Fragments arranged by size:
1705 377 252
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 938 971 2334
Size: 250 688 33 1363
Fragments arranged by size:
1363 688 250 33
BbvCI CC'TCA_GC
Cuts at: 0 1868 2334
Size: 1868 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 965 999 2334
Size: 965 34 1335
Fragments arranged by size:
1335 965 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1231 2334
Size: 1231 1103
BglI GCCn_nnn'nGGC
Cuts at: 0 598 2334
Size: 598 1736
BglII A'GATC_T
Cuts at: 0 345 2334
Size: 345 1989
BlpI GC'TnA_GC
Cuts at: 0 1615 2334
Size: 1615 719
Bme1580I G_kGCm'C
Cuts at: 0 666 2057 2334
Size: 666 1391 277
Fragments arranged by size:
1391 666 277
BmgBI CAC'GTC
Cuts at: 0 1893 1967 2334
Size: 1893 74 367
Fragments arranged by size:
1893 367 74
BmrI ACTGGGnnnn_n'
Cuts at: 0 1908 2334
Size: 1908 426
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1014 2334
Size: 1014 1320
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1006 2334
Size: 735 271 1328
Fragments arranged by size:
1328 735 271
Bpu10I CC'TnA_GC
Cuts at: 0 1568 1868 2334
Size: 1568 300 466
Fragments arranged by size:
1568 466 300
BsaI GGTCTCn'nnnn_
Cuts at: 0 185 1002 2334
Size: 185 817 1332
Fragments arranged by size:
1332 817 185
BsaHI Gr'CG_yC
Cuts at: 0 1837 2334
Size: 1837 497
BsaWI w'CCGG_w
Cuts at: 0 1312 1322 2334
Size: 1312 10 1012
Fragments arranged by size:
1312 1012 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 2334
Size: 37 2297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1907 2334
Size: 1907 427
BsiHKAI G_wGCw'C
Cuts at: 0 666 1312 1705 2334
Size: 666 646 393 629
Fragments arranged by size:
666 646 629 393
Bsp1286I G_dGCh'C
Cuts at: 0 666 1312 1705 1957 2057 2334
Size: 666 646 393 252 100 277
Fragments arranged by size:
666 646 393 277 252 100
BspEI T'CCGG_A
Cuts at: 0 1312 2334
Size: 1312 1022
BspHI T'CATG_A
Cuts at: 0 176 2334
Size: 176 2158
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2055 2334
Size: 2055 279
BsrDI GCAATG_nn'
Cuts at: 0 137 840 1236 2029 2334
Size: 137 703 396 793 305
Fragments arranged by size:
793 703 396 305 137
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2155 2334
Size: 2155 179
BstBI TT'CG_AA
Cuts at: 0 380 2334
Size: 380 1954
BstYI r'GATC_y
Cuts at: 0 345 654 1260 2334
Size: 345 309 606 1074
Fragments arranged by size:
1074 606 345 309
Bsu36I CC'TnA_GG
Cuts at: 0 937 2334
Size: 937 1397
BtgI C'CryG_G
Cuts at: 0 1264 1733 2334
Size: 1264 469 601
Fragments arranged by size:
1264 601 469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1502 1537 2334
Size: 1502 35 797
Fragments arranged by size:
1502 797 35
DraI TTT'AAA
Cuts at: 0 1391 2334
Size: 1391 943
DraIII CAC_nnn'GTG
Cuts at: 0 1298 2334
Size: 1298 1036
EarI CTCTTCn'nnn_
Cuts at: 0 1710 2192 2334
Size: 1710 482 142
Fragments arranged by size:
1710 482 142
EcoICRI GAG'CTC
Cuts at: 0 1703 2334
Size: 1703 631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 52 310 508 735 1006 1189 1622
Size: 33 19 258 198 227 271 183 433
Cuts at: 1622 1784 1807 2334
Size: 162 23 527
Fragments arranged by size:
527 433 271 258 227 198 183 162
33 23 19
EcoRI G'AATT_C
Cuts at: 0 1316 1691 2334
Size: 1316 375 643
Fragments arranged by size:
1316 643 375
EcoRV GAT'ATC
Cuts at: 0 814 2334
Size: 814 1520
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 364 1739 2334
Size: 364 1375 595
Fragments arranged by size:
1375 595 364
HaeII r_GCGC'y
Cuts at: 0 861 2334
Size: 861 1473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 545 577 1114 1146 2334
Size: 545 32 537 32 1188
Fragments arranged by size:
1188 545 537 32 32
HincII GTy'rAC
Cuts at: 0 2001 2334
Size: 2001 333
HindIII A'AGCT_T
Cuts at: 0 109 1393 2334
Size: 109 1284 941
Fragments arranged by size:
1284 941 109
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 650 940 2334
Size: 522 128 290 1394
Fragments arranged by size:
1394 522 290 128
MslI CAynn'nnrTG
Cuts at: 0 802 2140 2334
Size: 802 1338 194
Fragments arranged by size:
1338 802 194
MspA1I CmG'CkG
Cuts at: 0 1209 1238 2334
Size: 1209 29 1096
Fragments arranged by size:
1209 1096 29
NdeI CA'TA_TG
Cuts at: 0 1842 2334
Size: 1842 492
NspI r_CATG'y
Cuts at: 0 1020 2104 2334
Size: 1020 1084 230
Fragments arranged by size:
1084 1020 230
PfoI T'CCnGG_A
Cuts at: 0 1815 2334
Size: 1815 519
PsiI TTA'TAA
Cuts at: 0 1423 2334
Size: 1423 911
PvuII CAG'CTG
Cuts at: 0 1238 2334
Size: 1238 1096
SacI G_AGCT'C
Cuts at: 0 1705 2334
Size: 1705 629
SfcI C'TryA_G
Cuts at: 0 1981 2334
Size: 1981 353
SphI G_CATG'C
Cuts at: 0 1020 2334
Size: 1020 1314
SspI AAT'ATT
Cuts at: 0 1546 2334
Size: 1546 788
StyI C'CwwG_G
Cuts at: 0 1329 2016 2334
Size: 1329 687 318
Fragments arranged by size:
1329 687 318
TatI w'GTAC_w
Cuts at: 0 429 2334
Size: 429 1905
Tth111I GACn'n_nGTC
Cuts at: 0 1645 2334
Size: 1645 689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1222 1281 2334
Size: 1222 59 1053
Fragments arranged by size:
1222 1053 59
XmnI GAAnn'nnTTC
Cuts at: 0 376 2334
Size: 376 1958
Enzymes that do cut:
AcuI AflIII AlwNI ApaLI ApoI AseI BanI BanII
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI
BseRI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI
BstAPI BstBI BstYI Bsu36I BtgI CspCI DraI DraIII
EarI EcoICRI Eco57MI EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII MmeI MslI MspA1I NdeI NspI PfoI
PsiI PvuII SacI SfcI SphI SspI StyI TatI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BclI BfrBI BmtI BpuEI
BsaAI BsaBI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BsrBI BsrFI BsrGI BssHII BssSI BstEII BstXI BstZ17I
BtgZI BtsI ClaI DrdI EaeI EagI EciI EcoNI
EcoO109I FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI StuI SwaI TaqII TstI XbaI XhoI XmaI
ZraI