Restriction map for
pF1KA0044
(Linear) (Six-base) MAPSORT of: KIAA0044.seq Check: 594 from: 1 to: 1425
KIAA0044.orf
With 182 enzymes: SgfI *
March 3, 2008 10:54 ..
AccI GT'mk_AC
Cuts at: 0 755 1425
Size: 755 670
AclI AA'CG_TT
Cuts at: 0 346 1425
Size: 346 1079
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1171 1425
Size: 1171 254
AflII C'TTAA_G
Cuts at: 0 393 1425
Size: 393 1032
AflIII A'CryG_T
Cuts at: 0 275 1425
Size: 275 1150
AhdI GACnn_n'nnGTC
Cuts at: 0 860 1425
Size: 860 565
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 935 967 1425
Size: 935 32 458
Fragments arranged by size:
935 458 32
ApoI r'AATT_y
Cuts at: 0 319 385 544 925 1425
Size: 319 66 159 381 500
Fragments arranged by size:
500 381 319 159 66
AseI AT'TA_AT
Cuts at: 0 665 1425
Size: 665 760
AvaI C'yCGr_G
Cuts at: 0 497 1425
Size: 497 928
AvrII C'CTAG_G
Cuts at: 0 549 1425
Size: 549 876
BanII G_rGCy'C
Cuts at: 0 479 1008 1425
Size: 479 529 417
Fragments arranged by size:
529 479 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1050 1084 1425
Size: 1050 34 341
Fragments arranged by size:
1050 341 34
BclI T'GATC_A
Cuts at: 0 78 227 447 1425
Size: 78 149 220 978
Fragments arranged by size:
978 220 149 78
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1425
Size: 1425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 960 1425
Size: 395 565 465
Fragments arranged by size:
565 465 395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1425
Size: 576 849
BseYI C'CCAG_C
Cuts at: 0 764 1425
Size: 764 661
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 805 1425
Size: 805 620
BsiEI CG_ry'CG
Cuts at: 0 1422 1425
Size: 1422 3
BsiHKAI G_wGCw'C
Cuts at: 0 479 698 1008 1425
Size: 479 219 310 417
Fragments arranged by size:
479 417 310 219
Bsp1286I G_dGCh'C
Cuts at: 0 479 698 1008 1425
Size: 479 219 310 417
Fragments arranged by size:
479 417 310 219
BspHI T'CATG_A
Cuts at: 0 1034 1425
Size: 1034 391
BsrBI CCG'CTC
Cuts at: 0 316 1425
Size: 316 1109
BsrGI T'GTAC_A
Cuts at: 0 1192 1425
Size: 1192 233
BstBI TT'CG_AA
Cuts at: 0 282 1425
Size: 282 1143
BstYI r'GATC_y
Cuts at: 0 492 1305 1425
Size: 492 813 120
Fragments arranged by size:
813 492 120
Bsu36I CC'TnA_GG
Cuts at: 0 1352 1425
Size: 1352 73
BtgI C'CryG_G
Cuts at: 0 41 1425
Size: 41 1384
BtsI GCAGTG_nn'
Cuts at: 0 1386 1425
Size: 1386 39
DraIII CAC_nnn'GTG
Cuts at: 0 151 738 1425
Size: 151 587 687
Fragments arranged by size:
687 587 151
EaeI y'GGCC_r
Cuts at: 0 14 847 962 1419 1425
Size: 14 833 115 457 6
Fragments arranged by size:
833 457 115 14 6
EagI C'GGCC_G
Cuts at: 0 1419 1425
Size: 1419 6
EarI CTCTTCn'nnn_
Cuts at: 0 686 890 955 1162 1425
Size: 686 204 65 207 263
Fragments arranged by size:
686 263 207 204 65
EcoICRI GAG'CTC
Cuts at: 0 477 1006 1425
Size: 477 529 419
Fragments arranged by size:
529 477 419
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 960 1171 1425
Size: 395 565 211 254
Fragments arranged by size:
565 395 254 211
EcoO109I rG'GnC_Cy
Cuts at: 0 1356 1425
Size: 1356 69
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 106 723 1392 1425
Size: 106 617 669 33
Fragments arranged by size:
669 617 106 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 551 583 846 878 1425
Size: 551 32 263 32 547
Fragments arranged by size:
551 547 263 32 32
HincII GTy'rAC
Cuts at: 0 273 1425
Size: 273 1152
HindIII A'AGCT_T
Cuts at: 0 88 1425
Size: 88 1337
HpaI GTT'AAC
Cuts at: 0 273 1425
Size: 273 1152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1425
Size: 444 981
MscI TGG'CCA
Cuts at: 0 849 964 1425
Size: 849 115 461
Fragments arranged by size:
849 461 115
MslI CAynn'nnrTG
Cuts at: 0 623 1165 1425
Size: 623 542 260
Fragments arranged by size:
623 542 260
MspA1I CmG'CkG
Cuts at: 0 768 1425
Size: 768 657
NdeI CA'TA_TG
Cuts at: 0 260 1425
Size: 260 1165
NspI r_CATG'y
Cuts at: 0 279 1425
Size: 279 1146
PciI A'CATG_T
Cuts at: 0 275 1425
Size: 275 1150
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1425
Size: 42 1383
PpuMI rG'GwC_Cy
Cuts at: 0 1356 1425
Size: 1356 69
PstI C_TGCA'G
Cuts at: 0 1393 1425
Size: 1393 32
PvuII CAG'CTG
Cuts at: 0 768 1425
Size: 768 657
SacI G_AGCT'C
Cuts at: 0 479 1008 1425
Size: 479 529 417
Fragments arranged by size:
529 479 417
SapI GCTCTTCn'nnn_
Cuts at: 0 686 1162 1425
Size: 686 476 263
Fragments arranged by size:
686 476 263
SfcI C'TryA_G
Cuts at: 0 370 1389 1425
Size: 370 1019 36
Fragments arranged by size:
1019 370 36
SmlI C'TyrA_G
Cuts at: 0 393 555 1425
Size: 393 162 870
Fragments arranged by size:
870 393 162
SspI AAT'ATT
Cuts at: 0 650 1425
Size: 650 775
StyI C'CwwG_G
Cuts at: 0 549 1376 1425
Size: 549 827 49
Fragments arranged by size:
827 549 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 1425
Size: 226 1199
TatI w'GTAC_w
Cuts at: 0 1192 1425
Size: 1192 233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1374 1425
Size: 1374 51
XmnI GAAnn'nnTTC
Cuts at: 0 1156 1425
Size: 1156 269
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AloI ApoI
AseI AvaI AvrII BanII BcgI BclI BplI BpmI
BpuEI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrBI
BsrGI BstBI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII
HindIII HpaI MmeI MscI MslI MspA1I NdeI NspI
PciI PflMI PpuMI PstI PvuII SacI SapI SfcI
SmlI SspI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AleI AlfI AlwNI
ApaI ApaLI AscI AsiSI BaeI BamHI BanI BbeI
BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XbaI XhoI XmaI ZraI