Restriction map for
pF1KSDA0049
(Linear) (Six-base) MAPSORT of: KIAA0049.seq Check: 6537 from: 1 to: 2898
KIAA0049.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1519 2898
Size: 1519 1379
AccI GT'mk_AC
Cuts at: 0 1363 2898
Size: 1363 1535
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 790 1141 2063 2312 2522 2759 2836
Size: 581 209 351 922 249 210 237 77
Cuts at: 2836 2845 2898
Size: 9 53
Fragments arranged by size:
922 581 351 249 237 210 209 77
53 9
AfeI AGC'GCT
Cuts at: 0 200 2898
Size: 200 2698
AflII C'TTAA_G
Cuts at: 0 202 2898
Size: 202 2696
AhdI GACnn_n'nnGTC
Cuts at: 0 1680 2898
Size: 1680 1218
AleI CACnn'nnGTG
Cuts at: 0 1744 2898
Size: 1744 1154
AlwNI CAG_nnn'CTG
Cuts at: 0 1677 2412 2545 2719 2898
Size: 1677 735 133 174 179
Fragments arranged by size:
1677 735 179 174 133
ApoI r'AATT_y
Cuts at: 0 41 983 996 1134 2898
Size: 41 942 13 138 1764
Fragments arranged by size:
1764 942 138 41 13
AvaI C'yCGr_G
Cuts at: 0 1409 2898
Size: 1409 1489
BamHI G'GATC_C
Cuts at: 0 387 2898
Size: 387 2511
BanI G'GyrC_C
Cuts at: 0 254 2621 2898
Size: 254 2367 277
Fragments arranged by size:
2367 277 254
BanII G_rGCy'C
Cuts at: 0 796 1021 1459 1640 2150 2473 2567 2898
Size: 796 225 438 181 510 323 94 331
Fragments arranged by size:
796 510 438 331 323 225 181 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 382 829 1928 2898
Size: 382 447 1099 970
Fragments arranged by size:
1099 970 447 382
BbvCI CC'TCA_GC
Cuts at: 0 2087 2330 2898
Size: 2087 243 568
Fragments arranged by size:
2087 568 243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 383 417 2898
Size: 383 34 2481
Fragments arranged by size:
2481 383 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 177 2898
Size: 177 2721
BclI T'GATC_A
Cuts at: 0 1481 2547 2898
Size: 1481 1066 351
Fragments arranged by size:
1481 1066 351
BglI GCCn_nnn'nGGC
Cuts at: 0 2761 2898
Size: 2761 137
Bme1580I G_kGCm'C
Cuts at: 0 257 1733 2426 2898
Size: 257 1476 693 472
Fragments arranged by size:
1476 693 472 257
BmgBI CAC'GTC
Cuts at: 0 762 2898
Size: 762 2136
BmrI ACTGGGnnnn_n'
Cuts at: 0 277 1932 2898
Size: 277 1655 966
Fragments arranged by size:
1655 966 277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 532 850 997 1039 1206 2299 2372
Size: 493 39 318 147 42 167 1093 73
Cuts at: 2372 2666 2898
Size: 294 232
Fragments arranged by size:
1093 493 318 294 232 167 147 73
42 39
Bpu10I CC'TnA_GC
Cuts at: 0 2087 2330 2898
Size: 2087 243 568
Fragments arranged by size:
2087 568 243
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 422 1219 1285 1473 2601 2898
Size: 422 797 66 188 1128 297
Fragments arranged by size:
1128 797 422 297 188 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 774 2898
Size: 774 2124
BsaHI Gr'CG_yC
Cuts at: 0 1377 2898
Size: 1377 1521
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1345 1375 2639 2669 2898
Size: 1345 30 1264 30 229
Fragments arranged by size:
1345 1264 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 786 821 1760 2083 2122 2125 2128 2137
Size: 786 35 939 323 39 3 3 9
Cuts at: 2137 2146 2149 2152 2573 2898
Size: 9 3 3 421 325
Fragments arranged by size:
939 786 421 325 323 39 35 9
9 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1344 2314 2335 2704 2898
Size: 1344 970 21 369 194
Fragments arranged by size:
1344 970 369 194 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1136 1241 1440 2705 2898
Size: 418 718 105 199 1265 193
Fragments arranged by size:
1265 718 418 199 193 105
BsiHKAI G_wGCw'C
Cuts at: 0 1640 2150 2378 2473 2898
Size: 1640 510 228 95 425
Fragments arranged by size:
1640 510 425 228 95
BsmBI CGTCTCn'nnnn_
Cuts at: 0 469 2898
Size: 469 2429
Bsp1286I G_dGCh'C
Cuts at: 0 257 796 1021 1459 1640 1733 2150 2378
Size: 257 539 225 438 181 93 417 228
Cuts at: 2378 2426 2473 2567 2898
Size: 48 47 94 331
Fragments arranged by size:
539 438 417 331 257 228 225 181
94 93 48 47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1519 1685 2832 2898
Size: 1519 166 1147 66
Fragments arranged by size:
1519 1147 166 66
BsrDI GCAATG_nn'
Cuts at: 0 1337 1895 2639 2898
Size: 1337 558 744 259
Fragments arranged by size:
1337 744 558 259
BsrFI r'CCGG_y
Cuts at: 0 1306 2898
Size: 1306 1592
BsrGI T'GTAC_A
Cuts at: 0 2249 2481 2898
Size: 2249 232 417
Fragments arranged by size:
2249 417 232
BstAPI GCAn_nnn'nTGC
Cuts at: 0 656 912 2898
Size: 656 256 1986
Fragments arranged by size:
1986 656 256
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1402 2898
Size: 1402 1496
BstYI r'GATC_y
Cuts at: 0 387 1434 1656 2898
Size: 387 1047 222 1242
Fragments arranged by size:
1242 1047 387 222
BstZ17I GTA'TAC
Cuts at: 0 1364 2898
Size: 1364 1534
BtgI C'CryG_G
Cuts at: 0 1005 2898
Size: 1005 1893
BtsI GCAGTG_nn'
Cuts at: 0 401 2458 2717 2898
Size: 401 2057 259 181
Fragments arranged by size:
2057 401 259 181
DraI TTT'AAA
Cuts at: 0 33 1848 2898
Size: 33 1815 1050
Fragments arranged by size:
1815 1050 33
EaeI y'GGCC_r
Cuts at: 0 744 1308 2898
Size: 744 564 1590
Fragments arranged by size:
1590 744 564
EarI CTCTTCn'nnn_
Cuts at: 0 155 348 1445 1820 2596 2898
Size: 155 193 1097 375 776 302
Fragments arranged by size:
1097 776 375 302 193 155
EcoICRI GAG'CTC
Cuts at: 0 1638 2148 2471 2898
Size: 1638 510 323 427
Fragments arranged by size:
1638 510 427 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 532 581 790 850 997 1039 1141
Size: 493 39 49 209 60 147 42 102
Cuts at: 1141 1206 2063 2299 2312 2372 2522 2666 2759
Size: 65 857 236 13 60 150 144 93
Cuts at: 2759 2836 2845 2898
Size: 77 9 53
Fragments arranged by size:
857 493 236 209 150 147 144 102
93 77 65 60 60 53 49 42
39 13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 2898
Size: 148 2750
EcoO109I rG'GnC_Cy
Cuts at: 0 1674 1934 2694 2898
Size: 1674 260 760 204
Fragments arranged by size:
1674 760 260 204
EcoRI G'AATT_C
Cuts at: 0 996 2898
Size: 996 1902
EcoRV GAT'ATC
Cuts at: 0 90 2898
Size: 90 2808
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 217 1824 2068 2898
Size: 217 1607 244 830
Fragments arranged by size:
1607 830 244 217
FseI GG_CCGG'CC
Cuts at: 0 1310 2898
Size: 1310 1588
HaeII r_GCGC'y
Cuts at: 0 202 2898
Size: 202 2696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1666 1698 2751 2783 2898
Size: 1666 32 1053 32 115
Fragments arranged by size:
1666 1053 115 32 32
HincII GTy'rAC
Cuts at: 0 498 2898
Size: 498 2400
HindIII A'AGCT_T
Cuts at: 0 48 514 538 2898
Size: 48 466 24 2360
Fragments arranged by size:
2360 466 48 24
HpaI GTT'AAC
Cuts at: 0 498 2898
Size: 498 2400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1676 1700 2898
Size: 1676 24 1198
Fragments arranged by size:
1676 1198 24
MscI TGG'CCA
Cuts at: 0 746 2898
Size: 746 2152
MslI CAynn'nnrTG
Cuts at: 0 1744 2489 2627 2898
Size: 1744 745 138 271
Fragments arranged by size:
1744 745 271 138
MspA1I CmG'CkG
Cuts at: 0 635 2329 2799 2898
Size: 635 1694 470 99
Fragments arranged by size:
1694 635 470 99
NaeI GCC'GGC
Cuts at: 0 1308 2898
Size: 1308 1590
NcoI C'CATG_G
Cuts at: 0 1005 2898
Size: 1005 1893
NdeI CA'TA_TG
Cuts at: 0 741 2898
Size: 741 2157
NgoMIV G'CCGG_C
Cuts at: 0 1306 2898
Size: 1306 1592
NspI r_CATG'y
Cuts at: 0 1767 2898
Size: 1767 1131
PasI CC'CwG_GG
Cuts at: 0 2235 2898
Size: 2235 663
PpuMI rG'GwC_Cy
Cuts at: 0 1674 1934 2898
Size: 1674 260 964
Fragments arranged by size:
1674 964 260
PshAI GACnn'nnGTC
Cuts at: 0 2013 2898
Size: 2013 885
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 512 544 2898
Size: 512 32 2354
Fragments arranged by size:
2354 512 32
PstI C_TGCA'G
Cuts at: 0 236 396 2843 2898
Size: 236 160 2447 55
Fragments arranged by size:
2447 236 160 55
PvuII CAG'CTG
Cuts at: 0 635 2329 2799 2898
Size: 635 1694 470 99
Fragments arranged by size:
1694 635 470 99
SacI G_AGCT'C
Cuts at: 0 1640 2150 2473 2898
Size: 1640 510 323 425
Fragments arranged by size:
1640 510 425 323
SanDI GG'GwC_CC
Cuts at: 0 1934 2898
Size: 1934 964
SapI GCTCTTCn'nnn_
Cuts at: 0 2596 2898
Size: 2596 302
SbfI CC_TGCA'GG
Cuts at: 0 2843 2898
Size: 2843 55
ScaI AGT'ACT
Cuts at: 0 823 2898
Size: 823 2075
SfcI C'TryA_G
Cuts at: 0 232 392 2839 2898
Size: 232 160 2447 59
Fragments arranged by size:
2447 232 160 59
SmlI C'TyrA_G
Cuts at: 0 202 437 1234 1300 1488 2616 2898
Size: 202 235 797 66 188 1128 282
Fragments arranged by size:
1128 797 282 235 202 188 66
StuI AGG'CCT
Cuts at: 0 2276 2898
Size: 2276 622
StyI C'CwwG_G
Cuts at: 0 560 1005 1349 1973 2898
Size: 560 445 344 624 925
Fragments arranged by size:
925 624 560 445 344
TatI w'GTAC_w
Cuts at: 0 821 2249 2481 2898
Size: 821 1428 232 417
Fragments arranged by size:
1428 821 417 232
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 660 692 2639 2671 2898
Size: 660 32 1947 32 227
Fragments arranged by size:
1947 660 227 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2232 2898
Size: 2232 666
XmnI GAAnn'nnTTC
Cuts at: 0 1529 2858 2898
Size: 1529 1329 40
Fragments arranged by size:
1529 1329 40
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AleI AlwNI
ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BstZ17I BtgI BtsI DraI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NspI PasI PpuMI PshAI
PsrI PstI PvuII SacI SanDI SapI SbfI ScaI
SfcI SmlI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BfrBI
BglII BlpI BmtI BplI BsaAI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BssHII BssSI BstBI
BstEII Bsu36I BtgZI ClaI CspCI DraIII DrdI EagI
EciI FspI FspAI KasI KpnI MfeI MluI NarI
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI