Restriction map for
pF1KSDA0050
(Linear) (Six-base) MAPSORT of: KIAA0050.seq Check: 8783 from: 1 to: 2220
KIAA0050.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
Acc65I G'GTAC_C
Cuts at: 0 494 2220
Size: 494 1726
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 2033 2120 2220
Size: 1071 962 87 100
Fragments arranged by size:
1071 962 100 87
AflII C'TTAA_G
Cuts at: 0 774 2220
Size: 774 1446
AflIII A'CryG_T
Cuts at: 0 723 2220
Size: 723 1497
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 2220
Size: 1537 32 651
Fragments arranged by size:
1537 651 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 469 857 2220
Size: 333 136 388 1363
Fragments arranged by size:
1363 388 333 136
ApaI G_GGCC'C
Cuts at: 0 447 1496 1719 1927 2220
Size: 447 1049 223 208 293
Fragments arranged by size:
1049 447 293 223 208
ApoI r'AATT_y
Cuts at: 0 244 687 2220
Size: 244 443 1533
Fragments arranged by size:
1533 443 244
AvaI C'yCGr_G
Cuts at: 0 485 1115 2003 2220
Size: 485 630 888 217
Fragments arranged by size:
888 630 485 217
BanI G'GyrC_C
Cuts at: 0 146 437 494 661 1601 2220
Size: 146 291 57 167 940 619
Fragments arranged by size:
940 619 291 167 146 57
BanII G_rGCy'C
Cuts at: 0 221 447 1135 1174 1496 1682 1719 1927
Size: 221 226 688 39 322 186 37 208
Cuts at: 1927 1964 2004 2220
Size: 37 40 216
Fragments arranged by size:
688 322 226 221 216 208 186 40
39 37 37
BbeI G_GCGC'C
Cuts at: 0 150 441 665 1605 2220
Size: 150 291 224 940 615
Fragments arranged by size:
940 615 291 224 150
BglI GCCn_nnn'nGGC
Cuts at: 0 451 2220
Size: 451 1769
BglII A'GATC_T
Cuts at: 0 1448 2220
Size: 1448 772
BlpI GC'TnA_GC
Cuts at: 0 628 2188 2220
Size: 628 1560 32
Fragments arranged by size:
1560 628 32
Bme1580I G_kGCm'C
Cuts at: 0 127 447 510 1217 1496 1628 1719 1927
Size: 127 320 63 707 279 132 91 208
Cuts at: 1927 2220
Size: 293
Fragments arranged by size:
707 320 293 279 208 132 127 91
63
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 636 660 1248 1806 2220
Size: 130 506 24 588 558 414
Fragments arranged by size:
588 558 506 414 130 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 500 1701 2220
Size: 500 1201 519
Fragments arranged by size:
1201 519 500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 867 1003 1864 2220
Size: 421 446 136 861 356
Fragments arranged by size:
861 446 421 356 136
Bpu10I CC'TnA_GC
Cuts at: 0 1134 2220
Size: 1134 1086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 16 1683 2220
Size: 16 1667 537
Fragments arranged by size:
1667 537 16
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 136 1379 2220
Size: 88 48 1243 841
Fragments arranged by size:
1243 841 88 48
BsaAI yAC'GTr
Cuts at: 0 726 1218 1542 2220
Size: 726 492 324 678
Fragments arranged by size:
726 678 492 324
BsaHI Gr'CG_yC
Cuts at: 0 147 438 662 1602 2167 2220
Size: 147 291 224 940 565 53
Fragments arranged by size:
940 565 291 224 147 53
BsaWI w'CCGG_w
Cuts at: 0 1370 2220
Size: 1370 850
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1335 1365 1537 1567 1775 1805 2220
Size: 1335 30 172 30 208 30 415
Fragments arranged by size:
1335 415 208 172 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 637 772 1006 1867 2220
Size: 37 600 135 234 861 353
Fragments arranged by size:
861 600 353 234 135 37
BseYI C'CCAG_C
Cuts at: 0 490 657 747 1425 1495 2220
Size: 490 167 90 678 70 725
Fragments arranged by size:
725 678 490 167 90 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 927 1084 1690 1915 2220
Size: 927 157 606 225 305
Fragments arranged by size:
927 606 305 225 157
BsmI GAATG_Cn'
Cuts at: 0 1319 2220
Size: 1319 901
BsmBI CGTCTCn'nnnn_
Cuts at: 0 943 2122 2220
Size: 943 1179 98
Fragments arranged by size:
1179 943 98
Bsp1286I G_dGCh'C
Cuts at: 0 127 221 447 510 1135 1174 1217 1496
Size: 127 94 226 63 625 39 43 279
Cuts at: 1496 1628 1682 1719 1927 1964 2004 2220
Size: 132 54 37 208 37 40 216
Fragments arranged by size:
625 279 226 216 208 132 127 94
63 54 43 40 39 37 37
BspHI T'CATG_A
Cuts at: 0 2202 2220
Size: 2202 18
BsrDI GCAATG_nn'
Cuts at: 0 1073 1254 2220
Size: 1073 181 966
Fragments arranged by size:
1073 966 181
BsrFI r'CCGG_y
Cuts at: 0 632 2220
Size: 632 1588
BssSI C'ACGA_G
Cuts at: 0 695 2220
Size: 695 1525
BstAPI GCAn_nnn'nTGC
Cuts at: 0 469 1010 2220
Size: 469 541 1210
Fragments arranged by size:
1210 541 469
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2040 2220
Size: 2040 180
BstYI r'GATC_y
Cuts at: 0 323 1448 1666 2220
Size: 323 1125 218 554
Fragments arranged by size:
1125 554 323 218
BtgI C'CryG_G
Cuts at: 0 1472 1760 1779 2039 2220
Size: 1472 288 19 260 181
Fragments arranged by size:
1472 288 260 181 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2019 2220
Size: 2019 201
BtsI GCAGTG_nn'
Cuts at: 0 310 1484 2220
Size: 310 1174 736
Fragments arranged by size:
1174 736 310
DraIII CAC_nnn'GTG
Cuts at: 0 1221 2220
Size: 1221 999
EaeI y'GGCC_r
Cuts at: 0 1145 1184 1593 1651 1950 2220
Size: 1145 39 409 58 299 270
Fragments arranged by size:
1145 409 299 270 58 39
EarI CTCTTCn'nnn_
Cuts at: 0 452 697 820 1581 1757 1862 2220
Size: 452 245 123 761 176 105 358
Fragments arranged by size:
761 452 358 245 176 123 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 2220
Size: 244 1976
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 867 1003 1071 1864 2033 2120 2220
Size: 421 446 136 68 793 169 87 100
Fragments arranged by size:
793 446 421 169 136 100 87 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 337 1018 2220
Size: 337 681 1202
Fragments arranged by size:
1202 681 337
EcoO109I rG'GnC_Cy
Cuts at: 0 784 906 1492 1646 1692 1715 1716 1923
Size: 784 122 586 154 46 23 1 207
Cuts at: 1923 2022 2220
Size: 99 198
Fragments arranged by size:
784 586 207 198 154 122 99 46
23 1
EcoRI G'AATT_C
Cuts at: 0 687 2220
Size: 687 1533
HaeII r_GCGC'y
Cuts at: 0 150 441 665 1038 1605 2220
Size: 150 291 224 373 567 615
Fragments arranged by size:
615 567 373 291 224 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 979 1011 1775 1807 2220
Size: 979 32 764 32 413
Fragments arranged by size:
979 764 413 32 32
HincII GTy'rAC
Cuts at: 0 1794 2220
Size: 1794 426
HindIII A'AGCT_T
Cuts at: 0 771 2220
Size: 771 1449
KasI G'GCGC_C
Cuts at: 0 146 437 661 1601 2220
Size: 146 291 224 940 619
Fragments arranged by size:
940 619 291 224 146
KpnI G_GTAC'C
Cuts at: 0 498 2220
Size: 498 1722
MscI TGG'CCA
Cuts at: 0 1147 1186 1952 2220
Size: 1147 39 766 268
Fragments arranged by size:
1147 766 268 39
MslI CAynn'nnrTG
Cuts at: 0 1477 2038 2220
Size: 1477 561 182
Fragments arranged by size:
1477 561 182
MspA1I CmG'CkG
Cuts at: 0 678 1047 1499 1830 1846 1929 2220
Size: 678 369 452 331 16 83 291
Fragments arranged by size:
678 452 369 331 291 83 16
NaeI GCC'GGC
Cuts at: 0 634 2220
Size: 634 1586
NarI GG'CG_CC
Cuts at: 0 147 438 662 1602 2220
Size: 147 291 224 940 618
Fragments arranged by size:
940 618 291 224 147
NcoI C'CATG_G
Cuts at: 0 1472 1760 1779 2039 2220
Size: 1472 288 19 260 181
Fragments arranged by size:
1472 288 260 181 19
NgoMIV G'CCGG_C
Cuts at: 0 632 2220
Size: 632 1588
PasI CC'CwG_GG
Cuts at: 0 1167 1711 2109 2220
Size: 1167 544 398 111
Fragments arranged by size:
1167 544 398 111
PflMI CCAn_nnn'nTGG
Cuts at: 0 270 333 1239 2220
Size: 270 63 906 981
Fragments arranged by size:
981 906 270 63
PfoI T'CCnGG_A
Cuts at: 0 134 2220
Size: 134 2086
PmlI CAC'GTG
Cuts at: 0 726 1218 2220
Size: 726 492 1002
Fragments arranged by size:
1002 726 492
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 2220
Size: 1537 32 651
Fragments arranged by size:
1537 651 32
PpuMI rG'GwC_Cy
Cuts at: 0 906 1692 2022 2220
Size: 906 786 330 198
Fragments arranged by size:
906 786 330 198
PshAI GACnn'nnGTC
Cuts at: 0 334 935 2220
Size: 334 601 1285
Fragments arranged by size:
1285 601 334
PspOMI G'GGCC_C
Cuts at: 0 443 1492 1715 1923 2220
Size: 443 1049 223 208 297
Fragments arranged by size:
1049 443 297 223 208
PspXI vC'TCGA_Gb
Cuts at: 0 485 2220
Size: 485 1735
PstI C_TGCA'G
Cuts at: 0 317 527 1046 2220
Size: 317 210 519 1174
Fragments arranged by size:
1174 519 317 210
PvuII CAG'CTG
Cuts at: 0 678 1047 1499 1846 2220
Size: 678 369 452 347 374
Fragments arranged by size:
678 452 374 369 347
SanDI GG'GwC_CC
Cuts at: 0 2022 2220
Size: 2022 198
SfcI C'TryA_G
Cuts at: 0 313 523 1042 2220
Size: 313 210 519 1178
Fragments arranged by size:
1178 519 313 210
SfoI GGC'GCC
Cuts at: 0 148 439 663 1603 2220
Size: 148 291 224 940 617
Fragments arranged by size:
940 617 291 224 148
SmaI CCC'GGG
Cuts at: 0 1117 2220
Size: 1117 1103
SmlI C'TyrA_G
Cuts at: 0 31 485 774 1662 2003 2220
Size: 31 454 289 888 341 217
Fragments arranged by size:
888 454 341 289 217 31
SpeI A'CTAG_T
Cuts at: 0 1404 2220
Size: 1404 816
StuI AGG'CCT
Cuts at: 0 1522 2220
Size: 1522 698
StyI C'CwwG_G
Cuts at: 0 1302 1347 1472 1606 1760 1779 2039 2220
Size: 1302 45 125 134 154 19 260 181
Fragments arranged by size:
1302 260 181 154 134 125 45 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 739 2220
Size: 316 423 1481
Fragments arranged by size:
1481 423 316
TatI w'GTAC_w
Cuts at: 0 899 2220
Size: 899 1321
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 32 64 1335 1367 1817 1849 2220
Size: 32 32 1271 32 450 32 371
Fragments arranged by size:
1271 450 371 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2062 2220
Size: 2062 158
XbaI T'CTAG_A
Cuts at: 0 291 2220
Size: 291 1929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1240 1283 1345 2220
Size: 1240 43 62 875
Fragments arranged by size:
1240 875 62 43
XhoI C'TCGA_G
Cuts at: 0 485 2003 2220
Size: 485 1518 217
Fragments arranged by size:
1518 485 217
XmaI C'CCGG_G
Cuts at: 0 1115 2220
Size: 1115 1105
XmnI GAAnn'nnTTC
Cuts at: 0 1552 2220
Size: 1552 668
Enzymes that do cut:
Acc65I AcuI AflII AflIII AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I
BspHI BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI KpnI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PasI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SanDI SfcI SfoI SmaI SmlI SpeI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BcgI BciVI BclI BfrBI BmgBI BmtI
BsaBI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoICRI EcoRV FalI FseI FspI FspAI
HpaI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PsiI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SphI SrfI SspI SwaI
ZraI