Restriction map for
pF1KA0051
(Linear) (Six-base) MAPSORT of: KIAA0051.seq Check: 7478 from: 1 to: 4971
KIAA0051.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2347 2630 4557 4971
Size: 2347 283 1927 414
Fragments arranged by size:
2347 1927 414 283
AatII G_ACGT'C
Cuts at: 0 108 4971
Size: 108 4863
AccI GT'mk_AC
Cuts at: 0 227 4971
Size: 227 4744
Acc65I G'GTAC_C
Cuts at: 0 4481 4971
Size: 4481 490
AclI AA'CG_TT
Cuts at: 0 4167 4971
Size: 4167 804
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1531 2027 2335 3520 3571 3679 3808 4971
Size: 1531 496 308 1185 51 108 129 1163
Fragments arranged by size:
1531 1185 1163 496 308 129 108 51
AfeI AGC'GCT
Cuts at: 0 2470 4971
Size: 2470 2501
AflII C'TTAA_G
Cuts at: 0 3741 4971
Size: 3741 1230
AflIII A'CryG_T
Cuts at: 0 3035 4971
Size: 3035 1936
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 601 4295 4971
Size: 601 3694 676
Fragments arranged by size:
3694 676 601
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2264 2296 4971
Size: 2264 32 2675
Fragments arranged by size:
2675 2264 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1450 2442 2715 2922 4440 4863 4971
Size: 1050 400 992 273 207 1518 423 108
Fragments arranged by size:
1518 1050 992 423 400 273 207 108
ApoI r'AATT_y
Cuts at: 0 692 884 1540 1814 2133 2145 2290 2307
Size: 692 192 656 274 319 12 145 17
Cuts at: 2307 2611 3781 3832 4816 4971
Size: 304 1170 51 984 155
Fragments arranged by size:
1170 984 692 656 319 304 274 192
155 145 51 17 12
AseI AT'TA_AT
Cuts at: 0 671 1184 1634 1889 3752 4971
Size: 671 513 450 255 1863 1219
Fragments arranged by size:
1863 1219 671 513 450 255
AvaI C'yCGr_G
Cuts at: 0 2162 2552 4971
Size: 2162 390 2419
Fragments arranged by size:
2419 2162 390
AvrII C'CTAG_G
Cuts at: 0 171 4642 4971
Size: 171 4471 329
Fragments arranged by size:
4471 329 171
BamHI G'GATC_C
Cuts at: 0 2586 4971
Size: 2586 2385
BanI G'GyrC_C
Cuts at: 0 3181 3999 4481 4971
Size: 3181 818 482 490
Fragments arranged by size:
3181 818 490 482
BanII G_rGCy'C
Cuts at: 0 758 981 1349 3679 4971
Size: 758 223 368 2330 1292
Fragments arranged by size:
2330 1292 758 368 223
BbsI GAAGACnn'nnnn_
Cuts at: 0 562 1222 3388 4343 4971
Size: 562 660 2166 955 628
Fragments arranged by size:
2166 955 660 628 562
BbvCI CC'TCA_GC
Cuts at: 0 2582 4971
Size: 2582 2389
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 717 725 751 2143 2177 2443 2477
Size: 691 26 8 26 1392 34 266 34
Cuts at: 2477 4971
Size: 2494
Fragments arranged by size:
2494 1392 691 266 34 34 26 26
8
BciVI GTATCCnnnnn_n'
Cuts at: 0 463 1274 2266 2356 4971
Size: 463 811 992 90 2615
Fragments arranged by size:
2615 992 811 463 90
BclI T'GATC_A
Cuts at: 0 1385 2243 4442 4971
Size: 1385 858 2199 529
Fragments arranged by size:
2199 1385 858 529
BglII A'GATC_T
Cuts at: 0 180 3209 4971
Size: 180 3029 1762
Fragments arranged by size:
3029 1762 180
BlpI GC'TnA_GC
Cuts at: 0 794 4971
Size: 794 4177
Bme1580I G_kGCm'C
Cuts at: 0 1935 3186 4004 4971
Size: 1935 1251 818 967
Fragments arranged by size:
1935 1251 967 818
BmgBI CAC'GTC
Cuts at: 0 4110 4279 4971
Size: 4110 169 692
Fragments arranged by size:
4110 692 169
BmrI ACTGGGnnnn_n'
Cuts at: 0 250 2073 3214 3762 4971
Size: 250 1823 1141 548 1209
Fragments arranged by size:
1823 1209 1141 548 250
BmtI G_CTAG'C
Cuts at: 0 3367 4971
Size: 3367 1604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1139 2119 2737 4136 4971
Size: 223 916 980 618 1399 835
Fragments arranged by size:
1399 980 916 835 618 223
Bpu10I CC'TnA_GC
Cuts at: 0 214 1517 2582 3353 4971
Size: 214 1303 1065 771 1618
Fragments arranged by size:
1618 1303 1065 771 214
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 2857 4971
Size: 1723 1134 2114
Fragments arranged by size:
2114 1723 1134
BsaI GGTCTCn'nnnn_
Cuts at: 0 2095 2875 4198 4971
Size: 2095 780 1323 773
Fragments arranged by size:
2095 1323 780 773
BsaBI GATnn'nnATC
Cuts at: 0 1283 4971
Size: 1283 3688
BsaHI Gr'CG_yC
Cuts at: 0 105 4971
Size: 105 4866
BsaWI w'CCGG_w
Cuts at: 0 3951 4187 4971
Size: 3951 236 784
Fragments arranged by size:
3951 784 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1099 1129 1354 1384 2392 2422 4877 4907
Size: 1099 30 225 30 1008 30 2455 30
Cuts at: 4907 4971
Size: 64
Fragments arranged by size:
2455 1099 1008 225 64 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 220 877 1120 2182 2668 3067 4506
Size: 100 120 657 243 1062 486 399 1439
Cuts at: 4506 4971
Size: 465
Fragments arranged by size:
1439 1062 657 486 465 399 243 120
100
BseYI C'CCAG_C
Cuts at: 0 27 1145 1247 1727 4971
Size: 27 1118 102 480 3244
Fragments arranged by size:
3244 1118 480 102 27
BsiEI CG_ry'CG
Cuts at: 0 43 4971
Size: 43 4928
BsiHKAI G_wGCw'C
Cuts at: 0 1349 3959 4971
Size: 1349 2610 1012
Fragments arranged by size:
2610 1349 1012
BsmI GAATG_Cn'
Cuts at: 0 366 735 2342 3201 4131 4971
Size: 366 369 1607 859 930 840
Fragments arranged by size:
1607 930 859 840 369 366
BsmBI CGTCTCn'nnnn_
Cuts at: 0 96 4269 4971
Size: 96 4173 702
Fragments arranged by size:
4173 702 96
Bsp1286I G_dGCh'C
Cuts at: 0 758 981 1349 1935 3186 3679 3959 4004
Size: 758 223 368 586 1251 493 280 45
Cuts at: 4004 4971
Size: 967
Fragments arranged by size:
1251 967 758 586 493 368 280 223
45
BspEI T'CCGG_A
Cuts at: 0 3951 4971
Size: 3951 1020
BspHI T'CATG_A
Cuts at: 0 3369 4895 4971
Size: 3369 1526 76
Fragments arranged by size:
3369 1526 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2347 2411 2630 3068 4557 4606 4745 4871
Size: 2347 64 219 438 1489 49 139 126
Cuts at: 4871 4971
Size: 100
Fragments arranged by size:
2347 1489 438 219 139 126 100 64
49
BsrDI GCAATG_nn'
Cuts at: 0 3485 3680 3944 4451 4971
Size: 3485 195 264 507 520
Fragments arranged by size:
3485 520 507 264 195
BstAPI GCAn_nnn'nTGC
Cuts at: 0 652 1138 4971
Size: 652 486 3833
Fragments arranged by size:
3833 652 486
BstBI TT'CG_AA
Cuts at: 0 4802 4971
Size: 4802 169
BstXI CCAn_nnnn'nTGG
Cuts at: 0 201 2955 3003 4821 4971
Size: 201 2754 48 1818 150
Fragments arranged by size:
2754 1818 201 150 48
BstYI r'GATC_y
Cuts at: 0 180 2171 2586 3209 4373 4971
Size: 180 1991 415 623 1164 598
Fragments arranged by size:
1991 1164 623 598 415 180
Bsu36I CC'TnA_GG
Cuts at: 0 1872 4971
Size: 1872 3099
BtgI C'CryG_G
Cuts at: 0 365 2268 3177 3186 4412 4971
Size: 365 1903 909 9 1226 559
Fragments arranged by size:
1903 1226 909 559 365 9
BtsI GCAGTG_nn'
Cuts at: 0 1364 4971
Size: 1364 3607
ClaI AT'CG_AT
Cuts at: 0 3579 4971
Size: 3579 1392
DraI TTT'AAA
Cuts at: 0 4043 4388 4755 4971
Size: 4043 345 367 216
Fragments arranged by size:
4043 367 345 216
DraIII CAC_nnn'GTG
Cuts at: 0 1930 4971
Size: 1930 3041
DrdI GACnn_nn'nnGTC
Cuts at: 0 1079 4080 4971
Size: 1079 3001 891
Fragments arranged by size:
3001 1079 891
EaeI y'GGCC_r
Cuts at: 0 40 2228 2957 3189 4971
Size: 40 2188 729 232 1782
Fragments arranged by size:
2188 1782 729 232 40
EagI C'GGCC_G
Cuts at: 0 40 4971
Size: 40 4931
EarI CTCTTCn'nnn_
Cuts at: 0 145 746 1063 1753 2675 2887 4358 4971
Size: 145 601 317 690 922 212 1471 613
Fragments arranged by size:
1471 922 690 613 601 317 212 145
EcoICRI GAG'CTC
Cuts at: 0 1347 4971
Size: 1347 3624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 1139 1531 2027 2119 2335 2737 3520
Size: 223 916 392 496 92 216 402 783
Cuts at: 3520 3571 3679 3808 4136 4971
Size: 51 108 129 328 835
Fragments arranged by size:
916 835 783 496 402 392 328 223
216 129 108 92 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 972 1099 3994 4971
Size: 972 127 2895 977
Fragments arranged by size:
2895 977 972 127
EcoO109I rG'GnC_Cy
Cuts at: 0 1677 4734 4860 4971
Size: 1677 3057 126 111
Fragments arranged by size:
3057 1677 126 111
EcoRI G'AATT_C
Cuts at: 0 692 1540 2290 4971
Size: 692 848 750 2681
Fragments arranged by size:
2681 848 750 692
EcoRV GAT'ATC
Cuts at: 0 414 1560 2742 4971
Size: 414 1146 1182 2229
Fragments arranged by size:
2229 1182 1146 414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 1887 4569 4735 4971
Size: 543 1344 2682 166 236
Fragments arranged by size:
2682 1344 543 236 166
HaeII r_GCGC'y
Cuts at: 0 2472 3364 4971
Size: 2472 892 1607
Fragments arranged by size:
2472 1607 892
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1099 1131 1354 1386 3126 3158 3232 3264
Size: 1099 32 223 32 1740 32 74 32
Cuts at: 3264 3294 3326 3835 3867 4268 4300 4877 4909
Size: 30 32 509 32 401 32 577 32
Cuts at: 4909 4971
Size: 62
Fragments arranged by size:
1740 1099 577 509 401 223 74 62
32 32 32 32 32 32 32 32
30
HincII GTy'rAC
Cuts at: 0 21 525 4929 4971
Size: 21 504 4404 42
Fragments arranged by size:
4404 504 42 21
HindIII A'AGCT_T
Cuts at: 0 1565 2909 4971
Size: 1565 1344 2062
Fragments arranged by size:
2062 1565 1344
KpnI G_GTAC'C
Cuts at: 0 4485 4971
Size: 4485 486
MfeI C'AATT_G
Cuts at: 0 1432 4971
Size: 1432 3539
MluI A'CGCG_T
Cuts at: 0 3035 4971
Size: 3035 1936
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1787 1862 2885 3065 3908 4773 4804
Size: 545 1242 75 1023 180 843 865 31
Cuts at: 4804 4971
Size: 167
Fragments arranged by size:
1242 1023 865 843 545 180 167 75
31
MscI TGG'CCA
Cuts at: 0 2230 2959 3191 4971
Size: 2230 729 232 1780
Fragments arranged by size:
2230 1780 729 232
MslI CAynn'nnrTG
Cuts at: 0 583 2417 2507 3938 4971
Size: 583 1834 90 1431 1033
Fragments arranged by size:
1834 1431 1033 583 90
MspA1I CmG'CkG
Cuts at: 0 1251 1795 2220 2715 4476 4971
Size: 1251 544 425 495 1761 495
Fragments arranged by size:
1761 1251 544 495 495 425
NcoI C'CATG_G
Cuts at: 0 365 4971
Size: 365 4606
NheI G'CTAG_C
Cuts at: 0 3363 4971
Size: 3363 1608
NspI r_CATG'y
Cuts at: 0 170 435 655 1528 3414 3703 4971
Size: 170 265 220 873 1886 289 1268
Fragments arranged by size:
1886 1268 873 289 265 220 170
PacI TTA_AT'TAA
Cuts at: 0 1634 4971
Size: 1634 3337
PasI CC'CwG_GG
Cuts at: 0 996 4971
Size: 996 3975
PflMI CCAn_nnn'nTGG
Cuts at: 0 2097 2625 2715 4971
Size: 2097 528 90 2256
Fragments arranged by size:
2256 2097 528 90
PfoI T'CCnGG_A
Cuts at: 0 59 1781 2299 2495 4971
Size: 59 1722 518 196 2476
Fragments arranged by size:
2476 1722 518 196 59
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 1316 1348 4971
Size: 238 32 1046 32 3623
Fragments arranged by size:
3623 1046 238 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 4734 4860 4971
Size: 4734 126 111
Fragments arranged by size:
4734 126 111
PstI C_TGCA'G
Cuts at: 0 84 986 1016 1100 1115 1310 1676 1695
Size: 84 902 30 84 15 195 366 19
Cuts at: 1695 2258 2489 4871 4971
Size: 563 231 2382 100
Fragments arranged by size:
2382 902 563 366 231 195 100 84
84 30 19 15
PvuII CAG'CTG
Cuts at: 0 1251 1795 2220 2715 4971
Size: 1251 544 425 495 2256
Fragments arranged by size:
2256 1251 544 495 425
SacI G_AGCT'C
Cuts at: 0 1349 4971
Size: 1349 3622
SapI GCTCTTCn'nnn_
Cuts at: 0 746 4971
Size: 746 4225
ScaI AGT'ACT
Cuts at: 0 2491 3850 4971
Size: 2491 1359 1121
Fragments arranged by size:
2491 1359 1121
SexAI A'CCwGG_T
Cuts at: 0 1586 4971
Size: 1586 3385
SfcI C'TryA_G
Cuts at: 0 80 407 982 1012 1096 1111 1306 1672
Size: 80 327 575 30 84 15 195 366
Cuts at: 1672 1681 1691 2254 2485 4867 4873 4971
Size: 9 10 563 231 2382 6 98
Fragments arranged by size:
2382 575 563 366 327 231 195 98
84 80 30 15 10 9 6
SmlI C'TyrA_G
Cuts at: 0 1702 2872 3741 4971
Size: 1702 1170 869 1230
Fragments arranged by size:
1702 1230 1170 869
SpeI A'CTAG_T
Cuts at: 0 4506 4971
Size: 4506 465
SphI G_CATG'C
Cuts at: 0 170 3703 4971
Size: 170 3533 1268
Fragments arranged by size:
3533 1268 170
SspI AAT'ATT
Cuts at: 0 1464 4971
Size: 1464 3507
StuI AGG'CCT
Cuts at: 0 488 2240 4971
Size: 488 1752 2731
Fragments arranged by size:
2731 1752 488
StyI C'CwwG_G
Cuts at: 0 171 365 758 4642 4971
Size: 171 194 393 3884 329
Fragments arranged by size:
3884 393 329 194 171
SwaI ATTT'AAAT
Cuts at: 0 4043 4971
Size: 4043 928
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1351 1355 1864 2043 3928 4971
Size: 1351 4 509 179 1885 1043
Fragments arranged by size:
1885 1351 1043 509 179 4
TatI w'GTAC_w
Cuts at: 0 2489 3848 4971
Size: 2489 1359 1123
Fragments arranged by size:
2489 1359 1123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2744 2776 4971
Size: 2744 32 2195
Fragments arranged by size:
2744 2195 32
Tth111I GACn'n_nGTC
Cuts at: 0 1316 4971
Size: 1316 3655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 3000 4971
Size: 198 2802 1971
Fragments arranged by size:
2802 1971 198
XmnI GAAnn'nnTTC
Cuts at: 0 250 4971
Size: 250 4721
ZraI GAC'GTC
Cuts at: 0 106 4971
Size: 106 4865
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AlfI AloI AlwNI ApoI AseI AvaI AvrII
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I
HincII HindIII KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NheI NspI PacI PasI PflMI PfoI
PpiI PpuMI PstI PvuII SacI SapI ScaI SexAI
SfcI SmlI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AgeI AhdI AleI ApaI ApaLI AscI AsiSI BaeI
BbeI BfrBI BglI BplI BsaAI BsgI BsiWI BsrBI
BsrFI BsrGI BssHII BssSI BstEII BstZ17I BtgZI CspCI
EciI FseI FspI FspAI HpaI KasI NaeI NarI
NdeI NgoMIV NotI NruI NsiI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI XbaI XhoI XmaI