Restriction map for
pF1KSDA0053
(Linear) (Six-base) MAPSORT of: KIAA0053.seq Check: 5159 from: 1 to: 1914
KIAA0053.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 1149 1154 1914
Size: 157 992 5 760
Fragments arranged by size:
992 760 157 5
AfeI AGC'GCT
Cuts at: 0 457 1822 1914
Size: 457 1365 92
Fragments arranged by size:
1365 457 92
AleI CACnn'nnGTG
Cuts at: 0 658 1914
Size: 658 1256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 92 1914
Size: 92 1822
ApaLI G'TGCA_C
Cuts at: 0 661 1529 1914
Size: 661 868 385
Fragments arranged by size:
868 661 385
ApoI r'AATT_y
Cuts at: 0 409 487 1145 1381 1876 1914
Size: 409 78 658 236 495 38
Fragments arranged by size:
658 495 409 236 78 38
AvaI C'yCGr_G
Cuts at: 0 1826 1914
Size: 1826 88
BanI G'GyrC_C
Cuts at: 0 504 1345 1914
Size: 504 841 569
Fragments arranged by size:
841 569 504
BanII G_rGCy'C
Cuts at: 0 713 1914
Size: 713 1201
BbsI GAAGACnn'nnnn_
Cuts at: 0 962 1142 1914
Size: 962 180 772
Fragments arranged by size:
962 772 180
BbvCI CC'TCA_GC
Cuts at: 0 187 1914
Size: 187 1727
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 943 977 1914
Size: 943 34 937
Fragments arranged by size:
943 937 34
BclI T'GATC_A
Cuts at: 0 968 1013 1914
Size: 968 45 901
Fragments arranged by size:
968 901 45
BglII A'GATC_T
Cuts at: 0 1367 1914
Size: 1367 547
BlpI GC'TnA_GC
Cuts at: 0 785 1914
Size: 785 1129
Bme1580I G_kGCm'C
Cuts at: 0 509 665 1424 1533 1914
Size: 509 156 759 109 381
Fragments arranged by size:
759 509 381 156 109
BmrI ACTGGGnnnn_n'
Cuts at: 0 1601 1914
Size: 1601 313
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 654 998 1914
Size: 654 344 916
Fragments arranged by size:
916 654 344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 562 744 1082 1634 1786 1914
Size: 130 432 182 338 552 152 128
Fragments arranged by size:
552 432 338 182 152 130 128
Bpu10I CC'TnA_GC
Cuts at: 0 187 841 1406 1914
Size: 187 654 565 508
Fragments arranged by size:
654 565 508 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1424 1732 1914
Size: 1424 308 182
Fragments arranged by size:
1424 308 182
BsaI GGTCTCn'nnnn_
Cuts at: 0 692 1013 1914
Size: 692 321 901
Fragments arranged by size:
901 692 321
BsaWI w'CCGG_w
Cuts at: 0 1204 1230 1313 1914
Size: 1204 26 83 601
Fragments arranged by size:
1204 601 83 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 974 1914
Size: 412 562 940
Fragments arranged by size:
940 562 412
BseYI C'CCAG_C
Cuts at: 0 298 625 1121 1914
Size: 298 327 496 793
Fragments arranged by size:
793 496 327 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1914
Size: 548 1366
BsiEI CG_ry'CG
Cuts at: 0 552 1914
Size: 552 1362
BsiHKAI G_wGCw'C
Cuts at: 0 548 665 1533 1914
Size: 548 117 868 381
Fragments arranged by size:
868 548 381 117
BsmI GAATG_Cn'
Cuts at: 0 771 1914
Size: 771 1143
BsmBI CGTCTCn'nnnn_
Cuts at: 0 605 1914
Size: 605 1309
Bsp1286I G_dGCh'C
Cuts at: 0 509 548 665 713 1424 1533 1914
Size: 509 39 117 48 711 109 381
Fragments arranged by size:
711 509 381 117 109 48 39
BspEI T'CCGG_A
Cuts at: 0 1230 1914
Size: 1230 684
BspHI T'CATG_A
Cuts at: 0 971 1914
Size: 971 943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 845 1914
Size: 845 1069
BsrBI CCG'CTC
Cuts at: 0 633 1914
Size: 633 1281
BsrGI T'GTAC_A
Cuts at: 0 262 1914
Size: 262 1652
BssSI C'ACGA_G
Cuts at: 0 821 1914
Size: 821 1093
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 126 332 516 1271 1914
Size: 108 18 206 184 755 643
Fragments arranged by size:
755 643 206 184 108 18
BstYI r'GATC_y
Cuts at: 0 989 1367 1914
Size: 989 378 547
Fragments arranged by size:
989 547 378
Bsu36I CC'TnA_GG
Cuts at: 0 415 1914
Size: 415 1499
BtgI C'CryG_G
Cuts at: 0 713 1914
Size: 713 1201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1235 1914
Size: 1235 679
DraI TTT'AAA
Cuts at: 0 1374 1914
Size: 1374 540
DraIII CAC_nnn'GTG
Cuts at: 0 439 669 1914
Size: 439 230 1245
Fragments arranged by size:
1245 439 230
DrdI GACnn_nn'nnGTC
Cuts at: 0 364 1027 1914
Size: 364 663 887
Fragments arranged by size:
887 663 364
EaeI y'GGCC_r
Cuts at: 0 374 450 549 1914
Size: 374 76 99 1365
Fragments arranged by size:
1365 374 99 76
EagI C'GGCC_G
Cuts at: 0 549 1914
Size: 549 1365
EarI CTCTTCn'nnn_
Cuts at: 0 215 554 1039 1542 1613 1837 1914
Size: 215 339 485 503 71 224 77
Fragments arranged by size:
503 485 339 224 215 77 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 403 1914
Size: 403 1511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 157 562 744 1082 1149 1154 1634
Size: 130 27 405 182 338 67 5 480
Cuts at: 1634 1786 1914
Size: 152 128
Fragments arranged by size:
480 405 338 182 152 130 128 67
27 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1714 1914
Size: 1714 200
EcoO109I rG'GnC_Cy
Cuts at: 0 1508 1914
Size: 1508 406
EcoRI G'AATT_C
Cuts at: 0 1381 1914
Size: 1381 533
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 327 1914
Size: 327 1587
HaeII r_GCGC'y
Cuts at: 0 459 1824 1914
Size: 459 1365 90
Fragments arranged by size:
1365 459 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 251 283 1914
Size: 251 32 1631
Fragments arranged by size:
1631 251 32
HincII GTy'rAC
Cuts at: 0 891 1914
Size: 891 1023
HpaI GTT'AAC
Cuts at: 0 891 1914
Size: 891 1023
MscI TGG'CCA
Cuts at: 0 376 452 1914
Size: 376 76 1462
Fragments arranged by size:
1462 376 76
MslI CAynn'nnrTG
Cuts at: 0 124 514 658 1914
Size: 124 390 144 1256
Fragments arranged by size:
1256 390 144 124
MspA1I CmG'CkG
Cuts at: 0 108 606 876 1914
Size: 108 498 270 1038
Fragments arranged by size:
1038 498 270 108
NspI r_CATG'y
Cuts at: 0 249 1914
Size: 249 1665
PasI CC'CwG_GG
Cuts at: 0 237 1253 1513 1914
Size: 237 1016 260 401
Fragments arranged by size:
1016 401 260 237
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 1914
Size: 459 1455
PfoI T'CCnGG_A
Cuts at: 0 539 1914
Size: 539 1375
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1422 1454 1914
Size: 1422 32 460
Fragments arranged by size:
1422 460 32
PpuMI rG'GwC_Cy
Cuts at: 0 1508 1914
Size: 1508 406
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 724 756 1914
Size: 724 32 1158
Fragments arranged by size:
1158 724 32
PstI C_TGCA'G
Cuts at: 0 856 1791 1914
Size: 856 935 123
Fragments arranged by size:
935 856 123
PvuII CAG'CTG
Cuts at: 0 606 876 1914
Size: 606 270 1038
Fragments arranged by size:
1038 606 270
SanDI GG'GwC_CC
Cuts at: 0 1508 1914
Size: 1508 406
SexAI A'CCwGG_T
Cuts at: 0 593 1914
Size: 593 1321
SfcI C'TryA_G
Cuts at: 0 35 831 852 1115 1787 1914
Size: 35 796 21 263 672 127
Fragments arranged by size:
796 672 263 127 35 21
SmaI CCC'GGG
Cuts at: 0 1828 1914
Size: 1828 86
SmlI C'TyrA_G
Cuts at: 0 1439 1711 1914
Size: 1439 272 203
Fragments arranged by size:
1439 272 203
StuI AGG'CCT
Cuts at: 0 1855 1914
Size: 1855 59
StyI C'CwwG_G
Cuts at: 0 1046 1556 1856 1914
Size: 1046 510 300 58
Fragments arranged by size:
1046 510 300 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 32 1914
Size: 32 1882
TatI w'GTAC_w
Cuts at: 0 262 1914
Size: 262 1652
XmaI C'CCGG_G
Cuts at: 0 1826 1914
Size: 1826 88
XmnI GAAnn'nnTTC
Cuts at: 0 320 1321 1914
Size: 320 1001 593
Fragments arranged by size:
1001 593 320
Enzymes that do cut:
AcuI AfeI AleI AlfI ApaLI ApoI AvaI BanI
BanII BbsI BbvCI BcgI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrGI BssSI BstXI BstYI Bsu36I
BtgI BtgZI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HpaI MscI MslI MspA1I NspI PasI PflMI
PfoI PpiI PpuMI PsrI PstI PvuII SanDI SexAI
SfcI SmaI SmlI StuI StyI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BfrBI BglI BmgBI BmtI
BsaAI BsaBI BsaHI BsaXI BsiWI BsrDI BsrFI BssHII
BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI EcoICRI
EcoRV FseI FspI FspAI HindIII KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspOMI PspXI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI