Restriction map for
pF1KA0054
(Linear) (Six-base) MAPSORT of: KIAA0054.seq Check: 2303 from: 1 to: 5826
KIAA0054.orf
With 182 enzymes: SgfI *
August 5, 2008 16:25 ..
AccI GT'mk_AC
Cuts at: 0 240 4494 5826
Size: 240 4254 1332
Fragments arranged by size:
4254 1332 240
AclI AA'CG_TT
Cuts at: 0 1212 2023 5826
Size: 1212 811 3803
Fragments arranged by size:
3803 1212 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1574 1756 5333 5344 5551 5826
Size: 584 990 182 3577 11 207 275
Fragments arranged by size:
3577 990 584 275 207 182 11
AfeI AGC'GCT
Cuts at: 0 5694 5826
Size: 5694 132
AflII C'TTAA_G
Cuts at: 0 49 5826
Size: 49 5777
AflIII A'CryG_T
Cuts at: 0 243 1034 2787 5826
Size: 243 791 1753 3039
Fragments arranged by size:
3039 1753 791 243
AhdI GACnn_n'nnGTC
Cuts at: 0 5757 5826
Size: 5757 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 312 2573 5826
Size: 312 2261 3253
Fragments arranged by size:
3253 2261 312
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2584 2616 5826
Size: 2584 32 3210
Fragments arranged by size:
3210 2584 32
AlwNI CAG_nnn'CTG
Cuts at: 0 811 1458 4339 5775 5826
Size: 811 647 2881 1436 51
Fragments arranged by size:
2881 1436 811 647 51
ApaI G_GGCC'C
Cuts at: 0 5706 5826
Size: 5706 120
ApoI r'AATT_y
Cuts at: 0 732 1298 3184 3316 3516 3988 5826
Size: 732 566 1886 132 200 472 1838
Fragments arranged by size:
1886 1838 732 566 472 200 132
AseI AT'TA_AT
Cuts at: 0 3872 5826
Size: 3872 1954
AvaI C'yCGr_G
Cuts at: 0 3326 4461 5545 5826
Size: 3326 1135 1084 281
Fragments arranged by size:
3326 1135 1084 281
AvrII C'CTAG_G
Cuts at: 0 514 5781 5826
Size: 514 5267 45
Fragments arranged by size:
5267 514 45
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 944 977 3636 3669 5826
Size: 944 33 2659 33 2157
Fragments arranged by size:
2659 2157 944 33 33
BamHI G'GATC_C
Cuts at: 0 1914 5458 5826
Size: 1914 3544 368
Fragments arranged by size:
3544 1914 368
BanI G'GyrC_C
Cuts at: 0 586 3309 3618 5031 5826
Size: 586 2723 309 1413 795
Fragments arranged by size:
2723 1413 795 586 309
BanII G_rGCy'C
Cuts at: 0 3333 5249 5659 5706 5791 5826
Size: 3333 1916 410 47 85 35
Fragments arranged by size:
3333 1916 410 85 47 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 11 169 473 3292 3388 5826
Size: 11 158 304 2819 96 2438
Fragments arranged by size:
2819 2438 304 158 96 11
BbvCI CC'TCA_GC
Cuts at: 0 4187 4235 4250 4631 5826
Size: 4187 48 15 381 1195
Fragments arranged by size:
4187 1195 381 48 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 184 4546 4580 5826
Size: 150 34 4362 34 1246
Fragments arranged by size:
4362 1246 150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 342 3637 5826
Size: 342 3295 2189
Fragments arranged by size:
3295 2189 342
BclI T'GATC_A
Cuts at: 0 2463 2871 3414 4758 5826
Size: 2463 408 543 1344 1068
Fragments arranged by size:
2463 1344 1068 543 408
BfrBI ATG'CAT
Cuts at: 0 1179 5826
Size: 1179 4647
BglI GCCn_nnn'nGGC
Cuts at: 0 286 5121 5826
Size: 286 4835 705
Fragments arranged by size:
4835 705 286
BglII A'GATC_T
Cuts at: 0 1161 5826
Size: 1161 4665
BlpI GC'TnA_GC
Cuts at: 0 5474 5826
Size: 5474 352
Bme1580I G_kGCm'C
Cuts at: 0 591 5706 5826
Size: 591 5115 120
Fragments arranged by size:
5115 591 120
BmgBI CAC'GTC
Cuts at: 0 3961 5826
Size: 3961 1865
BmrI ACTGGGnnnn_n'
Cuts at: 0 585 1404 2209 2319 3255 3572 3974 5826
Size: 585 819 805 110 936 317 402 1852
Fragments arranged by size:
1852 936 819 805 585 402 317 110
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4476 4526 5826
Size: 4476 50 1300
Fragments arranged by size:
4476 1300 50
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 4603 5826
Size: 1508 3095 1223
Fragments arranged by size:
3095 1508 1223
Bpu10I CC'TnA_GC
Cuts at: 0 2036 4187 4235 4250 4631 5729 5826
Size: 2036 2151 48 15 381 1098 97
Fragments arranged by size:
2151 2036 1098 381 97 48 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 2226 4704 5315 5826
Size: 61 2165 2478 611 511
Fragments arranged by size:
2478 2165 611 511 61
BsaI GGTCTCn'nnnn_
Cuts at: 0 4702 5826
Size: 4702 1124
BsaAI yAC'GTr
Cuts at: 0 462 1035 2890 2989 5826
Size: 462 573 1855 99 2837
Fragments arranged by size:
2837 1855 573 462 99
BsaBI GATnn'nnATC
Cuts at: 0 905 5826
Size: 905 4921
BsaWI w'CCGG_w
Cuts at: 0 3859 4347 5826
Size: 3859 488 1479
Fragments arranged by size:
3859 1479 488
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 767 797 2584 2614 2711 2741 3590 3620
Size: 767 30 1787 30 97 30 849 30
Cuts at: 3620 4940 4970 5826
Size: 1320 30 856
Fragments arranged by size:
1787 1320 856 849 767 97 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1414 2732 3479 4238 4316 5003 5318 5826
Size: 1414 1318 747 759 78 687 315 508
Fragments arranged by size:
1414 1318 759 747 687 508 315 78
BseYI C'CCAG_C
Cuts at: 0 249 2195 4091 4938 5826
Size: 249 1946 1896 847 888
Fragments arranged by size:
1946 1896 888 847 249
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 4322 5826
Size: 69 4253 1504
Fragments arranged by size:
4253 1504 69
BsiHKAI G_wGCw'C
Cuts at: 0 791 3123 3166 5659 5791 5826
Size: 791 2332 43 2493 132 35
Fragments arranged by size:
2493 2332 791 132 43 35
BsmI GAATG_Cn'
Cuts at: 0 878 1726 3345 5826
Size: 878 848 1619 2481
Fragments arranged by size:
2481 1619 878 848
Bsp1286I G_dGCh'C
Cuts at: 0 591 791 3123 3166 3333 5249 5659 5706
Size: 591 200 2332 43 167 1916 410 47
Cuts at: 5706 5791 5826
Size: 85 35
Fragments arranged by size:
2332 1916 591 410 200 167 85 47
43 35
BspEI T'CCGG_A
Cuts at: 0 3859 4347 5826
Size: 3859 488 1479
Fragments arranged by size:
3859 1479 488
BspHI T'CATG_A
Cuts at: 0 3213 5826
Size: 3213 2613
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3594 5826
Size: 3594 2232
BsrBI CCG'CTC
Cuts at: 0 2600 5826
Size: 2600 3226
BsrDI GCAATG_nn'
Cuts at: 0 889 926 3152 3701 4400 5110 5460 5808
Size: 889 37 2226 549 699 710 350 348
Cuts at: 5808 5826
Size: 18
Fragments arranged by size:
2226 889 710 699 549 350 348 37
18
BsrGI T'GTAC_A
Cuts at: 0 2727 5142 5826
Size: 2727 2415 684
Fragments arranged by size:
2727 2415 684
BssSI C'ACGA_G
Cuts at: 0 2714 5826
Size: 2714 3112
BstAPI GCAn_nnn'nTGC
Cuts at: 0 937 1501 4057 5826
Size: 937 564 2556 1769
Fragments arranged by size:
2556 1769 937 564
BstBI TT'CG_AA
Cuts at: 0 2901 3882 5826
Size: 2901 981 1944
Fragments arranged by size:
2901 1944 981
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2822 3430 5826
Size: 2822 608 2396
Fragments arranged by size:
2822 2396 608
BstYI r'GATC_y
Cuts at: 0 1161 1914 3552 3736 5458 5826
Size: 1161 753 1638 184 1722 368
Fragments arranged by size:
1722 1638 1161 753 368 184
BstZ17I GTA'TAC
Cuts at: 0 4495 5826
Size: 4495 1331
Bsu36I CC'TnA_GG
Cuts at: 0 5519 5826
Size: 5519 307
BtgI C'CryG_G
Cuts at: 0 119 1879 2396 3634 5826
Size: 119 1760 517 1238 2192
Fragments arranged by size:
2192 1760 1238 517 119
BtsI GCAGTG_nn'
Cuts at: 0 81 296 4555 4695 5826
Size: 81 215 4259 140 1131
Fragments arranged by size:
4259 1131 215 140 81
ClaI AT'CG_AT
Cuts at: 0 1788 5826
Size: 1788 4038
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 324 359 1950 1985 5826
Size: 324 35 1591 35 3841
Fragments arranged by size:
3841 1591 324 35 35
DraI TTT'AAA
Cuts at: 0 5823 5826
Size: 5823 3
EaeI y'GGCC_r
Cuts at: 0 108 263 1952 2657 2812 4315 5589 5633
Size: 108 155 1689 705 155 1503 1274 44
Cuts at: 5633 5826
Size: 193
Fragments arranged by size:
1689 1503 1274 705 193 155 155 108
44
EarI CTCTTCn'nnn_
Cuts at: 0 6 1270 2789 5231 5737 5770 5826
Size: 6 1264 1519 2442 506 33 56
Fragments arranged by size:
2442 1519 1264 506 56 33 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 5826
Size: 493 5333
EcoICRI GAG'CTC
Cuts at: 0 5657 5789 5826
Size: 5657 132 37
Fragments arranged by size:
5657 132 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1508 1574 1756 4603 5333 5344 5551
Size: 584 924 66 182 2847 730 11 207
Cuts at: 5551 5826
Size: 275
Fragments arranged by size:
2847 924 730 584 275 207 182 66
11
EcoO109I rG'GnC_Cy
Cuts at: 0 93 136 4276 5254 5703 5720 5826
Size: 93 43 4140 978 449 17 106
Fragments arranged by size:
4140 978 449 106 93 43 17
EcoRI G'AATT_C
Cuts at: 0 732 5826
Size: 732 5094
EcoRV GAT'ATC
Cuts at: 0 457 5826
Size: 457 5369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 662 2302 3017 3058 5826
Size: 662 1640 715 41 2768
Fragments arranged by size:
2768 1640 715 662 41
HaeII r_GCGC'y
Cuts at: 0 4956 5497 5696 5826
Size: 4956 541 199 130
Fragments arranged by size:
4956 541 199 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 834 866 1210 1242 1350 1382
Size: 466 32 336 32 344 32 108 32
Cuts at: 1382 1781 1813 3913 3945 4170 4202 4545 4577
Size: 399 32 2100 32 225 32 343 32
Cuts at: 4577 5743 5775 5826
Size: 1166 32 51
Fragments arranged by size:
2100 1166 466 399 344 343 336 225
108 51 32 32 32 32 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1464 1534 3094 5826
Size: 1464 70 1560 2732
Fragments arranged by size:
2732 1560 1464 70
MfeI C'AATT_G
Cuts at: 0 1447 5826
Size: 1447 4379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 348 1892 2321 5516 5826
Size: 348 1544 429 3195 310
Fragments arranged by size:
3195 1544 429 348 310
MscI TGG'CCA
Cuts at: 0 110 265 1954 2659 2814 5591 5826
Size: 110 155 1689 705 155 2777 235
Fragments arranged by size:
2777 1689 705 235 155 155 110
MslI CAynn'nnrTG
Cuts at: 0 1861 2138 4502 5162 5826
Size: 1861 277 2364 660 664
Fragments arranged by size:
2364 1861 664 660 277
MspA1I CmG'CkG
Cuts at: 0 309 1980 3306 5826
Size: 309 1671 1326 2520
Fragments arranged by size:
2520 1671 1326 309
NcoI C'CATG_G
Cuts at: 0 119 1879 2396 3634 5826
Size: 119 1760 517 1238 2192
Fragments arranged by size:
2192 1760 1238 517 119
NdeI CA'TA_TG
Cuts at: 0 2139 2865 3291 5826
Size: 2139 726 426 2535
Fragments arranged by size:
2535 2139 726 426
NsiI A_TGCA'T
Cuts at: 0 1181 5826
Size: 1181 4645
NspI r_CATG'y
Cuts at: 0 41 247 306 1183 2472 5167 5826
Size: 41 206 59 877 1289 2695 659
Fragments arranged by size:
2695 1289 877 659 206 59 41
PasI CC'CwG_GG
Cuts at: 0 1641 4641 5826
Size: 1641 3000 1185
Fragments arranged by size:
3000 1641 1185
PciI A'CATG_T
Cuts at: 0 243 5826
Size: 243 5583
PflMI CCAn_nnn'nTGG
Cuts at: 0 1880 2397 5826
Size: 1880 517 3429
Fragments arranged by size:
3429 1880 517
PfoI T'CCnGG_A
Cuts at: 0 5768 5826
Size: 5768 58
PmlI CAC'GTG
Cuts at: 0 462 5826
Size: 462 5364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1106 1138 2584 2616 5826
Size: 1106 32 1446 32 3210
Fragments arranged by size:
3210 1446 1106 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 4276 5254 5826
Size: 4276 978 572
Fragments arranged by size:
4276 978 572
PshAI GACnn'nnGTC
Cuts at: 0 1588 5826
Size: 1588 4238
PsiI TTA'TAA
Cuts at: 0 2756 5826
Size: 2756 3070
PspOMI G'GGCC_C
Cuts at: 0 5702 5826
Size: 5702 124
PspXI vC'TCGA_Gb
Cuts at: 0 5545 5826
Size: 5545 281
PstI C_TGCA'G
Cuts at: 0 2060 3344 4335 5582 5826
Size: 2060 1284 991 1247 244
Fragments arranged by size:
2060 1284 1247 991 244
PvuII CAG'CTG
Cuts at: 0 309 1980 5826
Size: 309 1671 3846
Fragments arranged by size:
3846 1671 309
SacI G_AGCT'C
Cuts at: 0 5659 5791 5826
Size: 5659 132 35
Fragments arranged by size:
5659 132 35
SanDI GG'GwC_CC
Cuts at: 0 4276 5826
Size: 4276 1550
SapI GCTCTTCn'nnn_
Cuts at: 0 6 1270 5826
Size: 6 1264 4556
Fragments arranged by size:
4556 1264 6
ScaI AGT'ACT
Cuts at: 0 115 951 1121 2230 5826
Size: 115 836 170 1109 3596
Fragments arranged by size:
3596 1109 836 170 115
SexAI A'CCwGG_T
Cuts at: 0 5256 5826
Size: 5256 570
SfcI C'TryA_G
Cuts at: 0 183 576 1154 2056 3340 4331 4472 5568
Size: 183 393 578 902 1284 991 141 1096
Cuts at: 5568 5578 5826
Size: 10 248
Fragments arranged by size:
1284 1096 991 902 578 393 248 183
141 10
SmaI CCC'GGG
Cuts at: 0 3328 5826
Size: 3328 2498
SmlI C'TyrA_G
Cuts at: 0 49 76 2241 4719 5330 5545 5826
Size: 49 27 2165 2478 611 215 281
Fragments arranged by size:
2478 2165 611 281 215 49 27
SnaBI TAC'GTA
Cuts at: 0 2989 5826
Size: 2989 2837
SpeI A'CTAG_T
Cuts at: 0 436 5826
Size: 436 5390
SphI G_CATG'C
Cuts at: 0 306 1183 5826
Size: 306 877 4643
Fragments arranged by size:
4643 877 306
SspI AAT'ATT
Cuts at: 0 1070 5826
Size: 1070 4756
StyI C'CwwG_G
Cuts at: 0 119 266 514 1879 2396 3383 3634 3756
Size: 119 147 248 1365 517 987 251 122
Cuts at: 3756 5781 5826
Size: 2025 45
Fragments arranged by size:
2025 1365 987 517 251 248 147 122
119 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2104 2228 2552 3133 3396 3527 3782 5204
Size: 2104 124 324 581 263 131 255 1422
Cuts at: 5204 5514 5826
Size: 310 312
Fragments arranged by size:
2104 1422 581 324 312 310 263 255
131 124
TatI w'GTAC_w
Cuts at: 0 113 595 949 1119 1632 2228 2727 3456
Size: 113 482 354 170 513 596 499 729
Cuts at: 3456 4827 4870 5142 5826
Size: 1371 43 272 684
Fragments arranged by size:
1371 729 684 596 513 499 482 354
272 170 113 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 446 478 956 988 1562 1594 1958 1990
Size: 446 32 478 32 574 32 364 32
Cuts at: 1990 4835 4867 4940 4972 5826
Size: 2845 32 73 32 854
Fragments arranged by size:
2845 854 574 478 446 364 73 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 5258 5826
Size: 5258 568
XhoI C'TCGA_G
Cuts at: 0 5545 5826
Size: 5545 281
XmaI C'CCGG_G
Cuts at: 0 3326 5826
Size: 3326 2500
XmnI GAAnn'nnTTC
Cuts at: 0 2282 4441 5826
Size: 2282 2159 1385
Fragments arranged by size:
2282 2159 1385
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaI ApoI AseI AvaI AvrII BaeI
BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuII SacI SanDI SapI ScaI SexAI SfcI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AleI ApaLI AscI AsiSI
BbeI BmtI BsaHI BsiEI BsiWI BsmBI BsrFI BssHII
BstEII BtgZI DraIII DrdI EagI EcoNI FseI FspI
FspAI HincII HpaI KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI PacI PmeI PsrI PvuI
RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI
SrfI StuI SwaI XbaI XcmI ZraI