Restriction map for
pF1KA0055
(Linear) (Six-base) MAPSORT of: KIAA0055.seq Check: 3244 from: 1 to: 3354
KIAA0055.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 722 1065 3044 3354
Size: 722 343 1979 310
Fragments arranged by size:
1979 722 343 310
AccI GT'mk_AC
Cuts at: 0 931 3354
Size: 931 2423
AclI AA'CG_TT
Cuts at: 0 3036 3354
Size: 3036 318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 368 686 1274 1547 1940 2158 3278
Size: 142 226 318 588 273 393 218 1120
Cuts at: 3278 3354
Size: 76
Fragments arranged by size:
1120 588 393 318 273 226 218 142
76
AfeI AGC'GCT
Cuts at: 0 2143 3354
Size: 2143 1211
AflIII A'CryG_T
Cuts at: 0 2239 2812 3354
Size: 2239 573 542
Fragments arranged by size:
2239 573 542
AleI CACnn'nnGTG
Cuts at: 0 3179 3354
Size: 3179 175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 3354
Size: 162 3192
AlwNI CAG_nnn'CTG
Cuts at: 0 1153 1405 3354
Size: 1153 252 1949
Fragments arranged by size:
1949 1153 252
ApaLI G'TGCA_C
Cuts at: 0 109 3354
Size: 109 3245
ApoI r'AATT_y
Cuts at: 0 1835 2451 2485 3354
Size: 1835 616 34 869
Fragments arranged by size:
1835 869 616 34
BanII G_rGCy'C
Cuts at: 0 2970 3354
Size: 2970 384
BbsI GAAGACnn'nnnn_
Cuts at: 0 73 3354
Size: 73 3281
BbvCI CC'TCA_GC
Cuts at: 0 1620 3354
Size: 1620 1734
BciVI GTATCCnnnnn_n'
Cuts at: 0 1857 2581 3354
Size: 1857 724 773
Fragments arranged by size:
1857 773 724
BclI T'GATC_A
Cuts at: 0 2694 3252 3354
Size: 2694 558 102
Fragments arranged by size:
2694 558 102
BglII A'GATC_T
Cuts at: 0 125 2258 2646 3354
Size: 125 2133 388 708
Fragments arranged by size:
2133 708 388 125
BlpI GC'TnA_GC
Cuts at: 0 2111 3354
Size: 2111 1243
Bme1580I G_kGCm'C
Cuts at: 0 113 120 1766 3354
Size: 113 7 1646 1588
Fragments arranged by size:
1646 1588 113 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 3354
Size: 921 2433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 3354
Size: 1121 2233
Bpu10I CC'TnA_GC
Cuts at: 0 944 1620 3354
Size: 944 676 1734
Fragments arranged by size:
1734 944 676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 2247 3354
Size: 385 1862 1107
Fragments arranged by size:
1862 1107 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 2641 3354
Size: 520 2121 713
Fragments arranged by size:
2121 713 520
BsaAI yAC'GTr
Cuts at: 0 198 3354
Size: 198 3156
BsaBI GATnn'nnATC
Cuts at: 0 2995 3354
Size: 2995 359
BsaHI Gr'CG_yC
Cuts at: 0 966 1051 3354
Size: 966 85 2303
Fragments arranged by size:
2303 966 85
BsaWI w'CCGG_w
Cuts at: 0 353 821 2040 3354
Size: 353 468 1219 1314
Fragments arranged by size:
1314 1219 468 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2193 2223 3354
Size: 2193 30 1131
Fragments arranged by size:
2193 1131 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1292 2142 3354
Size: 1292 850 1212
Fragments arranged by size:
1292 1212 850
BseYI C'CCAG_C
Cuts at: 0 1961 3354
Size: 1961 1393
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2045 3354
Size: 2045 1309
BsiHKAI G_wGCw'C
Cuts at: 0 113 1917 2970 3354
Size: 113 1804 1053 384
Fragments arranged by size:
1804 1053 384 113
BsmI GAATG_Cn'
Cuts at: 0 151 623 3354
Size: 151 472 2731
Fragments arranged by size:
2731 472 151
Bsp1286I G_dGCh'C
Cuts at: 0 113 120 1766 1917 2970 3354
Size: 113 7 1646 151 1053 384
Fragments arranged by size:
1646 1053 384 151 113 7
BspEI T'CCGG_A
Cuts at: 0 353 821 3354
Size: 353 468 2533
Fragments arranged by size:
2533 468 353
BspHI T'CATG_A
Cuts at: 0 2498 3255 3354
Size: 2498 757 99
Fragments arranged by size:
2498 757 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 722 1047 1065 3044 3354
Size: 722 325 18 1979 310
Fragments arranged by size:
1979 722 325 310 18
BsrBI CCG'CTC
Cuts at: 0 2115 3354
Size: 2115 1239
BsrDI GCAATG_nn'
Cuts at: 0 507 879 2958 3354
Size: 507 372 2079 396
Fragments arranged by size:
2079 507 396 372
BsrGI T'GTAC_A
Cuts at: 0 2884 3354
Size: 2884 470
BssSI C'ACGA_G
Cuts at: 0 3332 3354
Size: 3332 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1405 2725 2960 3354
Size: 1405 1320 235 394
Fragments arranged by size:
1405 1320 394 235
BstYI r'GATC_y
Cuts at: 0 125 2258 2646 3354
Size: 125 2133 388 708
Fragments arranged by size:
2133 708 388 125
BstZ17I GTA'TAC
Cuts at: 0 932 3354
Size: 932 2422
BtsI GCAGTG_nn'
Cuts at: 0 689 2383 3354
Size: 689 1694 971
Fragments arranged by size:
1694 971 689
DraI TTT'AAA
Cuts at: 0 2550 2712 3354
Size: 2550 162 642
Fragments arranged by size:
2550 642 162
DraIII CAC_nnn'GTG
Cuts at: 0 1921 3354
Size: 1921 1433
EarI CTCTTCn'nnn_
Cuts at: 0 420 739 971 1250 1415 2180 2185 2665
Size: 420 319 232 279 165 765 5 480
Cuts at: 2665 3354
Size: 689
Fragments arranged by size:
765 689 480 420 319 279 232 165
5
EcoICRI GAG'CTC
Cuts at: 0 2968 3354
Size: 2968 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 368 686 1121 1274 1547 1940 2158
Size: 142 226 318 435 153 273 393 218
Cuts at: 2158 3278 3354
Size: 1120 76
Fragments arranged by size:
1120 435 393 318 273 226 218 153
142 76
EcoO109I rG'GnC_Cy
Cuts at: 0 173 3354
Size: 173 3181
EcoRI G'AATT_C
Cuts at: 0 1835 3354
Size: 1835 1519
EcoRV GAT'ATC
Cuts at: 0 3270 3354
Size: 3270 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 856 2632 3354
Size: 856 1776 722
Fragments arranged by size:
1776 856 722
FspI TGC'GCA
Cuts at: 0 871 3354
Size: 871 2483
HaeII r_GCGC'y
Cuts at: 0 2145 3354
Size: 2145 1209
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1115 1282 1314 3354
Size: 1083 32 167 32 2040
Fragments arranged by size:
2040 1083 167 32 32
HincII GTy'rAC
Cuts at: 0 1370 1751 3354
Size: 1370 381 1603
Fragments arranged by size:
1603 1370 381
HpaI GTT'AAC
Cuts at: 0 1370 3354
Size: 1370 1984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 679 1381 1750 2104 2236 3354
Size: 679 702 369 354 132 1118
Fragments arranged by size:
1118 702 679 369 354 132
MslI CAynn'nnrTG
Cuts at: 0 571 2468 2702 3179 3354
Size: 571 1897 234 477 175
Fragments arranged by size:
1897 571 477 234 175
MspA1I CmG'CkG
Cuts at: 0 396 3354
Size: 396 2958
NdeI CA'TA_TG
Cuts at: 0 2030 3354
Size: 2030 1324
NspI r_CATG'y
Cuts at: 0 2243 2728 2816 3354
Size: 2243 485 88 538
Fragments arranged by size:
2243 538 485 88
PciI A'CATG_T
Cuts at: 0 2239 2812 3354
Size: 2239 573 542
Fragments arranged by size:
2239 573 542
PflMI CCAn_nnn'nTGG
Cuts at: 0 1831 1987 3354
Size: 1831 156 1367
Fragments arranged by size:
1831 1367 156
PfoI T'CCnGG_A
Cuts at: 0 681 3354
Size: 681 2673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 3354
Size: 20 32 3302
Fragments arranged by size:
3302 32 20
PsiI TTA'TAA
Cuts at: 0 209 601 3354
Size: 209 392 2753
Fragments arranged by size:
2753 392 209
PstI C_TGCA'G
Cuts at: 0 2721 2956 3354
Size: 2721 235 398
Fragments arranged by size:
2721 398 235
SacI G_AGCT'C
Cuts at: 0 2970 3354
Size: 2970 384
SapI GCTCTTCn'nnn_
Cuts at: 0 1250 1415 3354
Size: 1250 165 1939
Fragments arranged by size:
1939 1250 165
SfcI C'TryA_G
Cuts at: 0 1064 1700 2717 2795 2952 3354
Size: 1064 636 1017 78 157 402
Fragments arranged by size:
1064 1017 636 402 157 78
SmlI C'TyrA_G
Cuts at: 0 364 2262 3354
Size: 364 1898 1092
Fragments arranged by size:
1898 1092 364
SspI AAT'ATT
Cuts at: 0 2372 3354
Size: 2372 982
StuI AGG'CCT
Cuts at: 0 2842 3354
Size: 2842 512
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1191 2028 3354
Size: 1191 837 1326
Fragments arranged by size:
1326 1191 837
TatI w'GTAC_w
Cuts at: 0 180 1279 2799 2884 3354
Size: 180 1099 1520 85 470
Fragments arranged by size:
1520 1099 470 180 85
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 265 499 3354
Size: 265 234 2855
Fragments arranged by size:
2855 265 234
XmnI GAAnn'nnTTC
Cuts at: 0 1025 2615 3354
Size: 1025 1590 739
Fragments arranged by size:
1590 1025 739
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaLI ApoI BanII BbsI BbvCI BciVI BclI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssSI BstAPI BstYI BstZ17I BtsI DraI DraIII
EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI MmeI MslI MspA1I NdeI
NspI PciI PflMI PfoI PpiI PsiI PstI SacI
SapI SfcI SmlI SspI StuI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AflII AgeI AhdI AloI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BfrBI BglI BmgBI BmtI BplI BsiEI BsiWI
BsmBI BsrFI BssHII BstBI BstEII BstXI Bsu36I BtgI
BtgZI ClaI CspCI DrdI EaeI EagI EciI EcoNI
FseI FspAI HindIII KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpuMI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StyI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI